Molecular Cloning,Expression and Characterization of Peroxisome Proliferators-Activated Receptors Gamma in the Sea Urchin(Strongylocentrotus intermedius)

Peroxisome proliferators-activated receptor gamma(PPARγ) plays important regulatory roles in adipocyte differentiation. In this study, we cloned the full-length sequence of the PPARγ gene and analyzed its expression profile in different developmental stages and tissues of Strongylocentrotus intermedius. The full-length cDNA of PPARγ contains 2286 base pairs(bp) with a putative open reading frame of 1755 bp, and the gene encodes encoding a polypeptide of 584 amino acid residues. The predicted molecular mass of the protein is 67.27 kDa, and its theoretical isoelectric point(pI) is 10.07. The protein contains conserved motifs, including an RRM(RNA recognition motif) domain. PPARγ expression with the highest level was observed in the gonad, and the lowest was observed in the tube feet of S. intermedius. Time-course expression measurements at different developmental stages showed that the highest expression level of PPARγ is in the eggs and its weakest expression level is in the 32-cells stage. Knock-down of PPARγ by specific siRNA revealed that UCP2 expression is significantly decreased in the gonads and intestines 48 h post-transfection, indicating that the UCP2 is a downstream target gene of PPARγ.This finding suggests that PPARγ and UCP2 have positive regulatory effects in regulating adipocyte differentiation. Changes in fatty acid levels in the gonads before and after PPARγ interference were assessed, and decreased C18:2(trans, n-6) and C20:3(n-6) levels were observed 48 h after siRNA transfection. The results showed the function of PPARγ in fatty acid anabolism, The data are helpful to improve the current understanding of the fatty acid synthesis pathways and regulatory mechanisms in S. intermedius. They also provide an experimental basis for improving fatty acid synthesis in sea urchins, which is important for cultivating sea urchins with high nutritional value.     

Late Cambrian to Early Silurian Granitic Rocks of the Gemuri Area, Central Qiangtang, North Tibet: New Constraints on the Tectonic Evolution of the Northern Margin of Gondwana

The Paleozoic tectonic framework and paleo–plate configuration of the northern margin of Gondwana remain controversial. The South Qiangtang terrane is located along the northern margin of Gondwana and records key processes in the formation and evolution of this supercontinent. Here, we present new field, petrological, zircon U-Pb geochronological, and Lu-Hf isotopic data for granitic rocks of the Gemuri pluton, all of which provide new insights into the evolution of the northern margin of Gondwana. Zircon U-Pb dating of the Gemuri pluton yielded three concordant ages of 488.5 ± 2.1, 479.9 ± 8.9, and 438.5 ± 3.5 Ma. Combining these ages with the results of previous research indicates that the South Qiangtang terrane records two magmatic episodes at 502–471 and 453–439 Ma. These two episodes are associated with enriched zircon Hf isotopic compositions(εHf(t) =-10.1 to-3.9 and-16.6 to-6.5, respectively), suggesting the granites were formed by the partial melting of Paleoproterozoic–Mesoproterozoic metasedimentary rocks(Two–stage Hf model ages(TCDM) = 2094–1704 and 2466–1827 Ma, respectively). Combining these data with the presence of linearly distributed, contemporaneous Paleozoic igneous rocks along the northern margin of Gondwana, we suggest that all of these rocks were formed in an active continental margin setting. This manifests that the two magmatic episodes within the Gemuri area were associated with southward subduction in the Proto-(Paleo-) Tethys Ocean.     

Spatial Variation of Dissolved Organic Carbon in Soils of Riparian Wetlands and Responses to Hydro-geomorphologic Changes in Sanjiang Plain,China

Spatial variation of dissolved organic carbon(DOC) in soils of riparian wetlands and responses to hydro-geomorphologic changes in the Sanjiang Plain were analyzed through in situ collecting soil samples in the Naoli River and the Bielahong River. The results showed that the average contents of DOC for soil layer of 0–100 cm were 730.6 mg/kg, 250.9 mg/kg, 423.0 mg/kg and 333.1 mg/kg respectively from riverbed to river terrace along the transverse directions of the Naoli watershed. The content of the soil DOC was the highest in the riverbed, lower in the high floodplain and much lower in the river terrace, and it was the lowest in the low floodplain. The difference in the content and vertical distribution of DOC between the riverbed and the three riparian wetlands was significant, while it was not significant among the low floodplain, the high floodplain and the river terrace. The variability of soil DOC was related to the hydrological connectivity between different landscape position of the riparian wetlands and the adjacent stream. Extremely significant correlations were observed between DOC and total organic carbon(TOC), total iron(TFe), ferrous iron(Fe(II)) whose correlation coefficients were 0.819, –0.544 and –0.709 in riparian wetlands of the Naoli River. With the increase of wetland destruction, soil p H increased and soil DOC content changed. The correlation coefficients between soil DOC and TOC, TFe, Fe(II) also changed into 0.759, –0.686 and –0.575 respectively in the Bielahong River. Under the impact of drainage ditches, the correlations between soil DOC and TFe, Fe(II) were not obvious, while the soil p H was weakly alkaline and was negatively correlated with soil DOC in the previous high floodplain. It indicates that riparian hydro-geomorphology is the main factor that could well explain this spatial variability of soil DOC, and the agricultural environmental hydraulic works like ditching also must be considered.     

Sulfur Isotope Geochemistry of the Xincheng Gold Deposit, Jiaodong: Constraints on the Material Source and Ore-forming Environment

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A Study of the Molecular Cloud S64 with Multiple Lines of CO Isotopes

We report on a study of the molecular cloud S64 with observations at millimeter wavelengths of multiple molecular lines of CO isotopes.A weak outflow is found,and its physical parameters are estimated.The departure of the core of S64 from the S64 HII region indicates that there are still other star formation activities in that region.     

Characteristics and variation law of wind-blown sand delivered to the Ningxia-Inner Mongolia reach of the Yellow River under a changing environment

Wind-blown sand is one of the key factors affecting the evolution of sediment transport,erosion,and deposition in rivers crossing desert areas.However,the differences and complex variations in the spatial and temporal distribution of the underlying surface conditions are seldom considered in research on the river inflow of wind-blown sand over a long time period.The Yellow River contains a large amount of sediment.The Ningxia-Inner Mongolia reach of the Yellow River was selected as the research ...     

Influence of fines content on the anti-frost properties of coarse-grained soil

This paper aims to determine the optimal fines content of coarse-grained soil required to simultaneously achieve weaker frost susceptibility and better bearing capacity. We studied the frost susceptibility and strength properties of coarse-grained soil by means of frost heaving tests and static triaxial tests, and the results are as follows:(1) the freezing temperature of coarse-grained soil decreased gradually and then leveled off with incremental increases in the percent content of fines; (2) the fines content proved to be an important factor influencing the frost heave susceptibility and strength properties of coarse-grained soil. With incremental increases in the percent content of fines, the frost heave ratio increased gradually and the cohesion function of fines effectively enhanced the shear strength of coarse-grained soil before freeze-thaw, but the frost susceptibility of fines weakened the shear strength of coarse-grained soil after freeze-thaw; (3) with increasing numbers of freeze-thaw cycles,the shear strength of coarse-grained soil decreased and then stabilized after the ninth freeze-thaw cycle, and therefore the mechanical indexes of the ninth freeze-thaw cycle are recommended for the engineering design values; and (4) considering frost susceptibility and strength properties as a whole, the optimal fines content of 5% is recommended for railway subgrade coarse-grained soil fillings in frozen regions.     

Monsoon Change in East Asia in the 21st Century: Results of RegCM3

The authors investigate monsoon change in East Asia in the 21st century under the Special Report on Emissions Scenarios (SRES) A1B scenario using the results of a regional climate model, RegCM3, with a high horizontal resolution. First, the authors evaluate the model’s performance compared with NCEP-NCAR reanalysis data, showing that the model can reliably reproduce the basic climatology of both winter and summer monsoons over East Asia. Next, it is found that the winter monsoon in East Asia would slightly weaken in the 21st century with spatial differences. Over northern East China, anomalous southerly winds would dominate in the mid-and late-21st century because the zonal land-sea thermal contrast is expected to become smaller, due to a stronger warming trend over land than over ocean. However, the intensity of the summer monsoon in East Asia shows a statistically significant upward trend over this century because the zonal land-sea thermal contrast between East Asia and the western North Pacific would become larger, which, in turn, would lead to larger sea level pressure gradients throughout East Asia and extending to the adjacent ocean.     

A 2D well-balanced,coupled model of water flow,sediment transport,and bed evolution based on unstructured grids with efficient variable storage strategy

In recent decades,a few Godunov-type,finite volume two-dimensional(2D)unstructured grid,coupled flow,and sediment models(GF2DUCM)have been developed for flows over erodible beds.These kinds of models are generally analyzed as a Vertex Model(VM)that define topography at the cell vertex,which can lead to the non-conservation of mass regarding flow,sediment,and bed evolution.Here,a full cellcantered variable storage method(Central Model or CM)is applied as the solution of the GF2DUCM.In this method,terrain elevation is defined at the cell centroids;this accurately describes the physical relations between the water depth and topography deformation.This approach can fully eliminate calculation errors in topography deformation at local cells caused by the interpolation of topography deformation at the cell vertex,and reduced uncertainty in the computation of the GF2DUCM.The model performance is systematically tested using a series of laboratory experiments,which demonstrate the mass conservation feature and high accuracy in reproducing hydrodynamic and morphological processes.     

A Quantitative Evaluation of Shale Gas Content in Different Occurrence States of the Longmaxi Formation: A New Insight from Well JY-A in the Fuling Shale Gas Field,Sichuan Basin

Comprehensive quantitative evaluation of shale gas content and the controlling factors in different occurrence states is of great significance for accurately assessing gas-bearing capacity and providing effective well-production strategies. A total of 122 core samples from well JY-A in the Fuling shale gas field were studied to reveal the characteristics of S_1 l shale,15 of which were selected to further predict the shale gas content in different occurrence states, which are dependent on geological factors in the thermal evolution process. Geological parameters were researched by a number of laboratory programs, and the factors influential in controlling shale gas content were extracted by both PCA and GRA methods and prediction models were confirmed by the BE method using SPSS software. Results reveal that the adsorbed gas content is mainly controlled by TOC, Ro, SSA, PD and pyrite content, and the free gas content is mainly controlled by S_2, quartz content, gas saturation and formation pressure for S_1 l in well JY-A. Three methods, including the on-site gas desorption method, the empirical formula method, and the multiple regression analysis method were used in combination to evaluate the shale gas capacity of well JY-A, all of which show that the overall shale gas content of well JY-A is in the range of 2.0–5.0 m~3/t and that the free gas ratio is about 50%, lower than that of well JY-1. Cause analysis further confirms the tectonics and preservation conditions of S_1 l in the geological processes, especially the influence of eastern boundary faults on well JY-A, as the fundamental reasons for the differences in shale gas enrichment in the Jiaoshiba area.