Microbial responses using denaturing gradient gel electrophoresis to oil and chemical dispersant in enclosed ecosystems

Microbial responses to the addition of oil with or without a chemical dispersant were examined in mesocosm and microcosm experiments by using denaturing gradient gel electrophoresis of bacterial ribosomal DNA and direct cell counting. When a water-soluble fraction of oil was added to seawater, increases in cell density were observed in the first 24h, followed by a decrease in abundance and a change in bacterial species composition. After addition of an oil-dispersant mixture, increases in cell density and changes in community structure coincided, and the amount of bacteria remained high. These phenomena also occurred in response to addition of only dispersant. Our results suggest that the chemical dispersant may be used as a nutrient source by some bacterial groups and may directly or indirectly prevent the growth of other bacterial groups.     

Improvement of pollutant drift forecast system applied to the Prestige oil spills in Galicia Coast (NW of Spain): development of an operational system

An integrated system named METEOMOHID, developed by MeteoGalicia in the first stage of the Prestige accident in November 2002 was used successfully in an operational form to support decision making and assist in recovering tasks. Afterwards, METEOMOHID has been enhanced with the aim of developing an operational oceanography system to be used in the NW of the Iberian Peninsula. The METEOMOHID system includes local area hydrodynamic coastal ocean modelling (MOHID), real time atmospheric forcing from a local meteorological model (ARPS). Using the available data from the Prestige crisis, a set of simulations were designed in order to reproduce the oil spill drift. The implementation of a detailed vertical resolution in the model has allowed obtaining a detailed surface dynamic, improving our knowledge of the behaviour of tarballs into the water column. Thus, the wind-driven Eckman drift, the direct dragging of the wind were detached, and the possible existence of subsurface oil was assessed. In addition, the present work evaluates the effects of introducing climatologic large scale currents in the METEOMOHID system.     

Biomarkers of exposure and effect in the Brazilian flounder Paralichthys orbignyanus (Teleostei: Paralichthyidae) from the Patos Lagoon estuary (Southern Brazil)

Biomarkers of exposure (liver metallothionein-like proteins content and catalase and glutathione S-transferase activities) and effect (liver lipoperoxidation and blood cell DNA damage) of contaminants were analyzed in the Brazilian flounder Paralichthys orbignyanus from the Patos Lagoon estuary (Southern Brazil). Flounders were collected for a year in two sites: "Coroa do Boi" (polluted site) and "Saco do Justino" (non-polluted site). Results indicated that micronucleated cells frequency was the best biomarker to distinguish flounders from the two sites. Taken together, data from DNA damage analyses (micronucleus test and comet assay) indicated that flounders from the non-polluted site efficiently repaired the DNA breaks, contrary to those from the polluted site, which probably had their DNA repair system inhibited or exhausted. Furthermore, data from enzyme activities (catalase and GST) and lipid peroxidation indicated that flounders from the polluted site were under oxidative stress in summer and autumn.     

Use of biliary PAH metabolites as a biomarker of pollution in fish from the Baltic Sea

During field campaigns of the BEEP project (Biological Effects of Environmental Pollution in Marine Coastal Ecosystems) in 2001-2002, metabolites of polycyclic aromatic hydrocarbons (PAHs) were determined in bile samples from three fish species, flounder (Platichthys flesus), perch (Perca fluviatilis) and eelpout (Zoarces viviparus), from four separate areas in the Baltic Sea. Two determination methods were applied: fixed wavelength fluorescence (FF) for pyrene-type metabolites and high-performance liquid chromatography with fluorescence detection (HPLC). There was a good correlation between the FF method and 1-OH pyrene determined by HPLC. Normalisation of the FF data for absorbance at 380 nm or bile protein concentrations greatly increased variance in one third and decreased it in two thirds of the cases and resulted in a loss of significant differences (protein normalisation) between the sampling stations, but normalisation of the HPLC data had little effect on the results. The biliary PAH metabolite content was usually higher in males than in females. In perch and eelpout the biliary PAH contents were at similar levels, whereas in flounder the levels were lower. The sampling areas arranged in decreasing order of biliary PAH contents were: Wismar Bay > Gulf of Gdansk > Lithuanian coast > Kvadofj?rden (reference area). It is concluded that FF with un-normalised data is a reliable and simple method for monitoring purposes and only one sex of a selected species should be used.     

Entanglement of New Zealand fur seals in man-made debris at Kaikoura, New Zealand

New Zealand fur seals in the Kaikoura region breed near a town with expanding tourist and fishing industries and commonly come ashore entangled in nets and plastic debris. However, the rate at which entanglement occurs was previously unknown. A decade of Department of Conservation seal callout data was analysed to determine the level of entanglement in the region and the most common debris type. Monitoring of adult female fur seals released from entanglement provided information on the potential for serious wounds to heal and survivorship of released individuals. Entanglement rates of pinnipeds in Kaikoura are some of the highest reported world-wide (average range: 0.6-2.8%) with green trawl net (42%), and plastic strapping tape (31%) together contributing the most to debris types. Nearly half of the reported entangled seals are successfully released (43%) and post-release monitoring shows that with appropriate intervention the chance of an individual surviving even with a significant entanglement wound is high. Our study demonstrates that while entanglement in the region is high, a successful intervention protocol may help reduce the potential for entanglement-related mortality in the region.     

Three-dimensional distributions of sewage markers in Tokyo Bay water--fluorescent whitening agents (FWAs)

Three-dimensional distributions of fluorescent whitening agents (FWAs: more specifically, DSBP and DAS1), which are sewage-derived water-soluble markers, were observed in Tokyo Bay water through multi-layer sampling of water at 20 locations. In summer, FWAs predominated in the surface layers, with trace but significant concentration of FWAs in bottom water due to stratification of seawater. In winter, on the other hand, FWAs were extensively mixed into the bottom layers because of the vertical mixing of seawater. In the surface layer, FWA concentrations and the DSBP/DAS1 ratio (the concentration ratio of DSBP to DAS1) were lower in summer than in winter, suggesting more efficient photodegradation of FWAs in euphotic zones during the summer due to stronger solar radiation. Horizontally, FWAs were widely distributed over the surface layer of Tokyo Bay. Surface water with DSBP concentrations above 50ng/L, corresponding to <200 times dilution of sewage effluent, was found to have spread up to 10km from the coastline. In addition, an offshore decline in FWA concentrations was observed, showing a half-distance of 10-20km. The decrease was caused by dilution by seawater of fresh water containing FWAs. The eastern part of the bay was different with respect to surface layers, with higher concentrations seen in northeastern parts. Furthermore, dispersion of combined sewer overflow (CSO)-derived water mass was observed in Tokyo Bay after heavy rain.     

Survey on germination and species composition of dinoflagellates from ballast tanks and recent sediments in ports on the South Coast of Finland, North-Eastern Baltic Sea

Cyst beds in ships and ports in Finland have previously been unstudied. Therefore, sediments from ships' ballast water tanks and four Finnish ports were sampled for dinoflagellate cysts and other phytoplankton. Untreated sediments were incubated at 10 degrees C and 20 degrees C in the local 6 psu salinity for 1, 4 and 7 days, and vegetative cells were examined with light and scanning electron microscope. Sediments were inhabited by various dinoflagellates, diatoms, chlorophytes, cyanophytes and small flagellates. Germinated dinoflagellates were found in 90% of ballast tanks and in all ports. Gymnodiniales spp. and Heterocapsa rotundata formed a major proportion of the proliferating dinoflagellate cells. One species, Peridinium quinquecorne, not previously reported from the Baltic Sea, was identified with SEM. The study emphasises that ships are potential transport vehicles for dinoflagellate cysts even in the low salinity Finnish waters, and small-sized dinoflagellates should be focused upon in ballast water studies.     

Advantages of doubly polished thin sections for the study of microfossils in volcanic rock

Doubly polished thin sections, originally prepared for fluid inclusion studies, present great advantages in the study of microfossils in volcanic rocks. Better visibility and light conditions, variation in thickness of the thin sections and the possibility to combine fluid inclusion studies with microfossil studies lead to a wide range of advantages over ordinary thin sections. This includes the study of morphology, internal microstructures, colonies, association with the substrate that microfossils are attached to and geological and environmental context in which the microfossil once lived. When meeting the criteria of microfossil recognition the advantages of doubly polished thin sections are substantial and can be crucial in distinguishing between biogenic microfossils and abiotically formed abiomorphs.     

A model test system with a dynamic load device for geotechnical engineering in cold regions

A model test system with a dynamic load device for geotechnical engineering in cold regions is presented. This system consists of a model test tank, a refrigeration device and temperature controller, a dynamic load device, together with sensors and data loggers for detecting stress, deformation, and temperature changes. The system can accommodate soil blocks up to 3 m in length, 2.5 m in width, and 1 m in height. The lowest temperature provided by the refrigeration device is -20 °C. The maximum load provided by the dynamic load device is 100 kN and the vibration frequency of the dynamic load can range from 0.1 to 10 Hz. A number of waveforms, such as sine waves, rectangular waves, triangle waves, and other user-defined waves can be generated by the dynamic load device controller.     

Snow water equivalent over Eurasia in the next 50 years projected by aggregated CMIP3 models

Based on remote sensing snow water equivalent (SWE) data, the simulated SWE in 20C3M experiments from 14 models attending the third phase of the Coupled Models for Inter-comparison Project (CMIP3) was first evaluated by computing the different percentage, spatial correlation coefficient, and standard deviation of biases during 1979–2000. Then, the diagnosed ten models that performed better simulation in Eurasian SWE were aggregated by arithmetic mean to project the changes of Eurasian SWE in 2002–2060. Results show that SWE will decrease significantly for Eurasia as a whole in the next 50 years. Spatially, significant decreasing trends dominate Eurasia except for significant increase in the northeastern part. Seasonally, decreasing proportion will be greatest in summer indicating that snow cover in warmer seasons is more sensitive to climate warming. However, absolute decreasing trends are not the greatest in winter, but in spring. This is caused by the greater magnitude of negative trends, but smaller positive trends in spring than in winter. The changing characteristics of increasing in eastern Eurasia and decreasing in western Eurasia and over the Qinghai-Tibetan Plateau favor the viewpoint that there will be more rainfall in North China and less in the middle and lower reaches of the Yangtze River in summer. Additionally, the decreasing rate and extent with significant decreasing trends under SRES A2 are greater than those under SRES B1, indicating that the emission of greenhouse gases (GHG) will speed up the decreasing rate of snow cover both temporally and spatially. It is crucial to control the discharge of GHG emissions for mitigating the disappearance of snow cover over Eurasia.