Assessment of phytoplankton class abundance using fluorescence excitation-emission matrix by parallel factor analysis and nonnegative least squares

The feasibility of using fluorescence excitation-emission matrix (EEM) along with parallel factor analysis (PARAFAC) and nonnegative least squares (NNLS) method for the differentiation of phytoplankton taxonomic groups was investigated. Forty-one phytoplankton species belonging to 28 genera of five divisions were studied. First, the PARAFAC model was applied to EEMs, and 15 fluorescence components were generated. Second, 15 fluorescence components were found to have a strong discriminating capability based on Bayesian discriminant analysis (BDA). Third, all spectra of the fluorescence component compositions for the 41 phytoplankton species were spectrographically sorted into 61 reference spectra using hierarchical cluster analysis (HCA), and then, the reference spectra were used to establish a database. Finally, the phytoplankton taxonomic groups was differentiated by the reference spectra database using the NNLS method. The five phytoplankton groups were differentiated with the correct discrimination ratios (CDRs) of 100% for single-species samples at the division level. The CDRs for the mixtures were above 91% for the dominant phytoplankton species and above 73% for the subdominant phytoplankton species. Sixteen of the 85 field samples collected from the Changjiang River estuary were analyzed by both HPLC-CHEMTAX and the fluorometric technique developed. The results of both methods reveal that Bacillariophyta was the dominant algal group in these 16 samples and that the subdominant algal groups comprised Dinophyta, Chlorophyta and Cryptophyta. The differentiation results by the fluorometric technique were in good agreement with those from HPLC-CHEMTAX. The results indicate that the fluorometric technique could differentiate algal taxonomic groups accurately at the division level.     

Study on deformation law of circular foundation under combined loading

The foundations of some ocean engineering structures are built to withstand not only the vertical gravity load V, but also the horizontal load H induced by sea waves and current. The horizontal load includes the concentrated force load, the moment load M, and the torque load T termed also as combined loading. It is of academic and engineering significance to study the deformation law of submarine seabed due to combined loading. On the basis of the three-dimensional elastic mechanics solution of circular foundation, numerical methods are used to analyze the deformation law of submarine soil under circular foundation with six degrees of freedom. The finite element analysis results give the elastic deformation law of soil in three dimensional spaces, modify the theoretical elasticity solution, and presents nonlinear soil deformation mechanism under the circular foundation with six degrees of freedom.     

Uptake and recovery of soil nitrogen by bryophytes and vascular plants in an alpine meadow

Due to their particular physiology and life history traits, bryophytes are critical in regulating biogeochemical cycles and functions in alpine ecosystem. Hence, it is crucial to investigate their nutrient utilization strategies in comparison with vascular plants and understand their responses to the variation of growing season caused by climate change. Firstly, this study testified whether or not bryophytes can absorb nitrogen(N) directly from soil through spiking three chemical forms of 15N stable isotope tracer. Secondly, with stronger ability of carbohydrates assimilation and photosynthesis, it is supposed that N utilization efficiency of vascular plants is significantly higher than that of bryophytes. However, the recovery of soil N by bryophytes can still compete with vascular plants due to their greater phytomass. Thirdly, resource acquisition may be varied from the change of growing season, during which N pulse can be manipulated with 15N tracer addition at different time. Both of bryophytes and vascular plants contain more N in a longer growing season, and prefer inorganic over organic N. Bryophytes assimilate more NH4+ than NO3– and amino acid, which can be indicated from the greater shoot excess 15N of bryophytes. However, vascular plants prefer to absorb NO3– for their developed root systems and vascular tissue. Concerning the uptake of three forms N by bryophytes, there is significant difference between two manipulated lengths of growing season. Furthermore, the capacity of bryophytes to tolerate N-pollution may be lower than currently appreciated, which indicates the effect of climate change on asynchronous variation of soil N pools with plant requirements.     

Properties and organic activation of ocean clay in the East Pacific

In order to make use of ocean clay reasonably and excavate its potential applications the properties of ocean clay in Chinese investigating area of the East Pacific have been tested with the chemical analysis, XRFS, ICP/MAS, granulometry and SSA, etc. The results show that the clay sediments of this area are rich in clay minerals, fine granularity, bigger SSA, bigger water absorbability and lower ion changing ability etc. To improve the adaptability of ocean clay and macromolecule materials, using the dry milling method with silane, aluminate, stearic acid coupling agents etc the properties of ocean clay are improved. The effect has been evaluated by testing the water absorbing of the modified ocean clay and their dispersing in paraffin liquid. The result is that KH550 is better than any other active reagents, and the best activation technics is that 1.5% (volume) of KH550 quantity, 20min of milling time. The DTA, XRD, SEM and IR analyses and SSA testing show that the organic activation can not change the mineral structure but can densify the structure of clay, and activation mechanism is chemical covered mainly and chemical combined secondly.     

Synthetic information prediction system for crisis mine based on GIS

Reserves of some kinds of the crisis mines will be lack now or from now on, because of lacking seriously reserves of mineral resources and the crisis of exploring bases in support. So that it is urgent to predict, appraise, development and utilize the replaceable resources of the crisis mines. The mineral resources prediction software system of synthetic information is intelligent GIS which is used to quantitative prediction of large-scale synthetic information mineral target. It takes the geological body and the mineral resource body as a unit. And it analyzes the ore deposit genesis and metallotect, knows the spatial distribution laws of the ore deposit and ore body, and establish the prospecting model based on the concept of establishing the three-dimensional space of a mine. This paper will primarily discuss some important problems as follows: the secondary development of various kinds of data(including geology, geophysical prospecting, geochemical prospecting and remote sensing, etc); process synthetically and establish the synthetic information interpretative map base; correspond prospecting model with synthetic information of ore deposit; divided into statistical units of metallogenic information synthetic anomalies based on the synthetic information anomalies of ore control, then research the metallogenic information variable of unit synthetically and make quantitative prediction according to choose the quantitative prediction math model which is suitable to the demands of large-scale precision; at last, finish the target area optimization of ore deposit (body).     

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A sub-surface eddy at inertial current layer in the Canada Basin,Arctic Ocean

An Arctic Ocean eddy in sub-surface layer is analyzed in this paper by use of temperature,salinity and current profiles data obtained at an ice camp in the Canada Basin during the second Chinese Arctic Expedition in summer of 2003.In the vertical temperature section,the eddy shows itself as an isolated cold water block at depth of 60 m with a minimum temperature of-1.5℃,about 0.5℃ colder than the ambient water.Isopycnals in the eddy form a pattern of convex,which indicates the eddy is anticyclonic.Although maximum velocity near 0.4 m s-1 occurs in the current records observed synchronously,the current pattern is far away from a typical eddy.By further analysis,inertial frequency oscillations with amplitudes comparable with the eddy velocity are found in the sub-surface layer currents.After filter the inertial current and mean current,an axisymmetric current pattern of an eddy with maximum velocity radius of 5 km is obtained.The analysis of the T-S characteristics of the eddy core water and its ambient waters supports the conclusion that the eddy was formed on the Chukchi Shelf and migrated northeastward into the northern Canada Basin.     

Design ofVibrio 16S rRNA gene specific primers and their application in the analysis of seawaterVibrio community

The pathogenic species of genus Vibrio cause vibriosis, one of the most prevalent diseases of maricultured animals and seafood consumers. Monitoring their kinetics in the chain of seafood production, processing and consumption is of great importance for food and mariculture safety. In order to enrich Vibrio-representing 16S ribosomal RNA gene （rDNA） fragments and identify these bacteria further real-timely and synchronously among bacterial flora in the chain, a pair of primers that selectively amplify Vibrio 16S rDNA fragments were designed with their specificities and coverage testified in the analysis of seawater Vibrio community. The specificities and coverage of two primers, VF169 and VR744, were determined theoretically among bacterial 16S rDNAs available in GenBank by using BLAST program and practically by amplifying Vibrio 16S rDNA fragments from seawater DNA. More than 88.3% of sequences in GenBank, which showed identical matches with VR744, belong to Vibrio genus. A total of 33 clones were randomly selected and sequenced. All of the sequences showed their highest similarities to and clustered around those of diverse known Vibrio species. The primers designed are capable of retrieving a wide range of Vibrio 16S rDNA fragments specifically among bacterial flora in seawater, the most important natural environment of seafood cultivation.     

Geographical impacts on social networks from perspectives of space and place: an empirical study using mobile phone data

Space and place are two fundamental concepts in geography. Geographical factors have long been known as drivers of many aspects of people’s social networks. But whether and how space and place affect social networks differently are still unclear. The widespread use of location-aware devices provides a novel source for distinguishing the mechanisms of their impacts on social networks. Using mobile phone data, this paper explores the effects of space and place on social networks. From the perspective of space, we confirm the distance decay effect in social networks, based on a comparison between synthetic social ties generated by a null model and actual social ties derived from real-world data. From the perspective of place, we introduce several measures to evaluate interactions between individuals and inspect the trio relationship including distance, spatio-temporal co-occurrence, and social ties. We found that people’s interaction is a more important factor than spatial proximity, indicating that the spatial factor has a stronger impact on social networks in place compared to that in space. Furthermore, we verify the hypothesis that interactions play an important role in strengthening friendships.     

高空间分辨率遥感影像最优分割结果自动确定方法EI北大核心CSCD

针对现有方法普遍存在不能充分顾及遥感影像多波段光谱信息,以及忽视遥感影像中地理要素的多尺度特性等问题,提出一种自动确定高空间分辨率遥感影像最优分割结果的非监督评价方法。该方法基于信息熵生成光谱信息离散度,利用光谱信息离散度构建能表达分割对象内部光谱均质性指标和分割对象与其相邻分割对象间光谱异质性指标。基于构建的光谱均质性和光谱异质性指标,采用“粗估计+精确定”的策略,逐步得到一个多级优化后的影像最优分割结果。本文在3个不同下垫面影像区域进行试验。结果表明,该方法能有效地实现自动确定高空间分辨率遥感影像最优分割结果,与现有方法相比,本文方法确定出的影像最优分割结果质量更高,与参考分割结果更加贴近。