In order to thoroughly investigate the diversity of glacier microorganisms, four DNA extraction methods with different lysis patterns
were tested and two screened methods (the Bosshard-Bano method and the Zhou method) were optimized for the most effective
form of the filter membrane (cut vs. uncut), the DNA extraction method, and the precipitation method. The two optimized
methods were then compared with the commercial Mo-Bio DNA extraction kit, and the results showed that the kit was generally
suitable for extraction of microorganism DNA from glacier surface snow. Procedurally, it was found that a modified Bosshard-
Bano method (i.e., cutting the filter membrane into pieces, using a specific lysis pattern [lysozyme (5 mg/mL)-protease K
(1 mg/mL)-CTAB (1%)-SDS (1%)], performing the extraction only once by chloroform-isoamyl alcohol (24:1), and conducting
DNA precipitation by pure ethanol) was also an effective and less expensive method for extraction of microorganism DNA from
glacier surface snow. 相似文献
Land use change is one of the important aspects of global change[1-3]. The Bohai Rim is a hot spot of economic development in China, where land use changes remarkably. Analyzing land use change in this area is significant to the research of global change and regional sustainable land use. Although there has been much work undertaken on regional land use change in China, it was mainly on urban land use change[4-11], and few researches integrated remote sensing and GIS method. Based on data f… 相似文献