Isolation and characterization of 49 polymorphic microsatellite loci for Decapterus maruadsi using SLAF-seq,and cross-amplification to related species

Decapterus maruadsi is a commercially important species in China, but has been heavily exploited in some areas. There is a growing need to develop microsatellites promoting its genetic research for the adequate management of this fishery resources. The recently developed specific-locus amplified fragment sequencing (SLAF-seq) is an efficient and high-resolution method for genome-wide microsatellite markers discovery. In this study, 28 905 microsatellites (mono- to hexa-nucleotide repeats) were identified using SLAF-seq technology, of which di-nucleotide was the most frequent (13 590, 47.02%), followed by mono-nucleotide (8 138, 28.15%), tri-nucleotide (5 727, 19.81%), tetra-nucleotide (1 104, 3.82%), pentanucleotide (234, 0.81%), and hexa-nucleotide (112, 0.39%). One hundred and thirty-two microsatellite loci (di- and tri-nucleotide) were randomly selected for amplification and polymorphism, of which 49 were highly polymorphic and well-resolved. The average number of alleles per locus was 13.63, ranging from 4 to 25, and allele sizes varied between 110 bp and 309 bp. The observed heterozygosity ( H_o ) and expected heterozygosity ( H_e ) ranged from 0.233 to 1.000 and from 0.374 to 0.959, with mean values of 0.738 and 0.836, respectively. The polymorphism information content (PIC) ranged from 0.341 to 0.941 (mean=0.806). However, 12 loci deviated from Hardy-Weinberg equilibrium. Furthermore, transferability tests were also successful in validating the utility of the developed markers in five phylogenetically related species of family Carangidae. A total of 48 microsatellite markers were successfully cross-amplified in Decapterus macarellus, Decapterus macrosoma, Decapterus kurroides, Trachurus japonicus, and Selaroides leptolepis. The present microsatellites provided the first known set of microsatellite DNA markers for D. maruadsi, D. macarellus, D. kurroides, and D. macrosoma, and would be useful for further population genetic and molecular phylogeny studies as well as help with the fisheries management formulation and implementation of the understudied species.

     

A strip adjustment procedure to mitigate the impact of inaccurate mounting parameters in parallel lidar strips

Lidar (laser scanning) technology has been proven as a prominent technique for the acquisition of high-density and accurate topographic information. Because of systematic errors in the lidar measurements (drifts in the position and orientation information and biases in the mirror angles and ranges) and/or in the parameters relating the system components (mounting parameters), adjacent lidar strips may exhibit discrepancies. Although position and orientation drifts can have a more significant impact, these errors and their impact do not come as a surprise if the quality of the GPS/INS integration process is carefully examined. Therefore, the mounting errors are singled out in this work. The ideal solution for improving the compatibility of neighbouring strips in the presence of errors in the mounting parameters is the implementation of a rigorous calibration procedure. However, such a calibration requires the original observations, which may not be usually available. In this paper, a strip adjustment procedure to improve the compatibility between parallel lidar strips with moderate flight dynamics (for example, acquired by a fixed-wing aircraft) over an area with moderately varying elevation is proposed. The proposed method is similar to the photogrammetric block adjustment of independent models. Instead of point features, planar patches and linear features, which are represented by sets of non-conjugate points, are used for the strip adjustment. The feasibility and the performance of the proposed procedure together with its impact on subsequent activities are illustrated using experimental results from real data.     

柄海鞘对铅的生物富集作用

应用半静态的双箱式生物富集动力学模型,在室内研究了柄海鞘对重金属 Pb2+ 的生物富集作用,通过非线性拟合得到了相应的生物富集动力学参数.结果表明:柄海鞘对金属 Pb2+ 具有一定的富集作用,对 Pb2+ 的富集吸收速率常数 k1 和富集系数 BCF均随外部水体中 Pb2+ 浓度的增大而减小.富集平衡时,海鞘体内的铅含? Camax)与外部水体中 Pb2+ 的浓度基本呈正相关.柄海鞘不同器官中的 Pb 浓度不同,依次为:生殖腺>消化腺≈其他部分>外背囊.排出阶段 Pb 在柄海鞘体内的生物学半衰期(B1/2)为 10.05～36.76d.     

苏皖下扬子区晚白垩世以来的构造-热历史：浦口组砂岩磷灰石裂变径迹证据

对苏皖下扬子区上白垩统浦口组（K2P）的三个砂岩样品进行了磷灰石裂变径迹（AFT）研究。结果显示：2个样品的AFT合并年龄为（88．8±4．4）Ma（径迹长度为（12．0±0．3）μm）和（82．1±6．8）Ma（径迹长度为（14．4±0．3）μm）与浦口组沉积年龄相近,说明它们沉降的幅度达到但没有超过AFT部分退火区间,1个样品的AFT合并年龄（117．3±5．9）Ma（径迹长度为（13．3±0．3）μm）大于浦口组沉积年龄,代表物源区抬升、剥露的冷却年龄。根据热历史模拟结果,识别出黄桥事件（110～90Ma）、仪征事件（70～60Ma）和三垛事件（35～22Ma）三期重要的构造事件,并将下扬子区晚白垩世以来的盆地演化划分为四个阶段：110～70Ma断坳复合型伸展盆地、70～35Ma拉张断陷盆地、35～22Ma挤压抬升阶段和22Ma至今坳陷盆地。     

Authigenic iron oxide formation in the estuarine mixing zone of the Yangtze River

Estuaries are elementary geochemical fronts where river water and seawater mix. Within this mixing zone, iron and other non-conservative elements can undergo complex reactions to form new solid phases. In order to understand authigenic iron oxide formation in the Yangtze River Estuary, two onsite water-mixing sets of experiments were conducted, one by mixing variable amounts of unfiltered Yangtze River water with filtered East China Sea water of different salinity (set 1), the other by mixing variable amounts of filtered Yangtze River water with filtered East China Sea water of different salinity (set 2). In set 2, the minerals newly formed in the course of mixing were investigated by means of a scanning electron microscope fitted with an energy-dispersive X-ray analytical system. It was found that ferrihydrite and lepidocrocite were formed in these mixing experiments, coexisting in nearly equal amounts. These iron oxides appear as aggregated particles with a large grain-size range of several microns to more than 100 μm. The electrolytic properties of seawater played an important role in the formation of these authigenic iron oxides. Kaolinite and organic aggregates were also found in the experimentally mixed pre-filtered waters. Amounts of newly formed suspended matter (set 2) were one to three orders of magnitude lower than those of total suspended matter (TSM) (set 1). This implies that newly formed minerals represent only a very small proportion of TSM in the estuarine mixing zone of the Yangtze River.     

2009年冬、夏季南海北部超微型浮游生物的分布特征及其环境相关分析

2009年2月(冬季)和8月(夏季)在南海北部海域(nSCS)采用流式细胞术对聚球藻、原绿球藻、超微型光合真核生物3类超微型光合浮游生物和异养浮游细菌的丰度和碳生物量的时空分布特征进行了研究,并分析了其与环境因子之间的关系。结果表明,夏季聚球藻和原绿球藻的平均丰度高于冬季,超微型光合真核生物和异养浮游细菌的丰度反之,为冬季高于夏季。聚球藻、超微型光合真核生物和异养浮游细菌在富营养的近岸陆架海域丰度较高,而原绿球藻高丰度则出现在陆坡开阔海域。在垂直分布上,聚球藻主要分布在跃层以上,跃层以下丰度迅速降低;原绿球藻高丰度主要出现在真光层底部;超微型光合真核生物在水层中的高值同样出现在真光层底部,且与Pico级份叶绿素a浓度分布一致;异养浮游细菌在水体中的分布与聚球藻类似。这些分布格局的差异,取决于环境条件的变化和4类超微型浮游生物生态生理适应性的差异。在超微型光合浮游生物群落中,各类群碳生物量的贡献因季节和海域类型的不同而发生变化:聚球藻在夏季近岸陆架区占超微型光合浮游生物总碳生物量的41%,原绿球藻在陆坡开阔海成为主要贡献者(50%),超微型光合真核生物碳生物量以冬季为高(在近岸陆架区占比68%)。冬、夏季异养浮游细菌碳生物量均高于超微型光合浮游生物碳生物量。     

Pore-water geochemistry in methane-seep sediments of the Makran accretionary wedge off Pakistan: Possible link to subsurface methane hydrate

Cold seeps are pervasive along the continental margin worldwide,and are recognized as hotspots for elemental cycling pathway on Earth.In this study,analyses of pore water geochemical compositions of one-400 cm piston core(S3) and the application of a mass balance model are conducted to assess methane-associated biogeochemical reactions and uncover the relationship of methane in shallow sediment with gas hydrate reservoir at the Makran accretionary wedge off Pakistan.The results revealed that approximately 77% of sulfate is consumed by the predominant biogeochemical process of anaerobic oxidation of methane.However,the estimated sulfate-methane interface depth is-400 cm below sea floor with the methane diffusive flux of 0.039 mol/(m~2·a),suggesting the activity of methane seepage.Based on the δ~(13)C_(DIC) mass balance model combined with the contribution proportion of different dissolved inorganic carbon sources,this study calculated the δ~(13)C of the exogenous methane to be-57.9‰,indicating that the exogenous methane may be a mixture source,including thermogenic and biogenic methane.The study of pore water geochemistry at Makran accretionary wedge off Pakistan may have considerable implications for understanding the specific details on the dynamics of methane in cold seeps and provide important evidence for the potential occurrence of subsurface gas hydrate in this area.     

p-y曲线对成层土体中大直径单桩的适用性研究

API规范推荐的p-y曲线是由均质土体得到的,并未考虑土层间相互作用,Georgiadis基于柔性桩提出了等效深度法修正p-y曲线,把均质土p-y曲线延伸到了成层土体中。为了研究p-y曲线和等效深度法对于大直径单桩在成层土体中的适用性,取4种典型地质条件:成层砂土、砂土-黏土-砂土、成层黏土和黏土-砂土-黏土,通过L-PILE软件计算了6 m直径单桩基础的p-y曲线、桩顶水平荷载-位移曲线、桩身位移和弯矩。并与ABAQUS建立的单桩基础三维有限元模型计算结果进行比较。结果表明等效深度法对于成层砂土影响不大;对于成层黏土影响较大;对于中间为软弱土层的成层土体,在荷载较大时影响显著,等效深度法计算结果更加接近FEM结果。在成层土体中,p-y曲线应用于大直径单桩对于砂土高估了初始刚度,低估了极限抗力;对于黏土则低估了初始刚度和极限抗力。     

Extension of high-order harmonics and generation of an isolated attosecond pulse in the chirped laser field

This paper theoretically investigates the high-order harmonic generation cutoff extension using intense few-cycle linearly chirped laser pulses. It shows that the cutoff of the harmonic can be extended remarkably by optimising the chirping parameters. The time-frequency characteristics of high-order harmonics with different chirping parameters are analysed by means of wavelet transform of the dipole acceleration. It also gives out the classical three-step model pictures of electron. By superposing a properly selected range of the harmonic spectrum, it obtains an isolated 65as pulse.     

1987—2018年西双版纳橡胶林时空变化及其线状特征

基于1987—2018年西双版纳30 m分辨率橡胶林数据集,利用叠置、缓冲区等GIS空间分析方法,对比分析了过去32年橡胶林沿道路、河流和边境线的空间变化特征与区域差异。结果表明：1) 1987—2018年,西双版纳橡胶种植面积在道路、河流沿线5 km以内分别增加了1 874.6和1 484.6 km²,在边境地区(国境线20km缓冲带)增加了1 393.1 km²。2)从道路沿线看,受橡胶种植可达性和交通运输影响,过去32年道路5 km缓冲带内的橡胶林平均占比超过九成。3)从河流沿线看,因灌溉和水源等需求,过去32年河流沿线5 km缓冲带内橡胶林平均占比69.7%。值得注意的是,受天然橡胶价格持续低迷、城镇化与生态环境保护等影响,2010年河流沿线橡胶林种植面积开始下降,沿线2 km内表现尤为显著。4)从边境地区看,西双版纳橡胶林种植呈明显趋边性特征,跨境种植频繁。过去32年边境沿线地区橡胶林平均占比55.6%,且由1987年的35.4%上升到2018年的66.2%,其中,在中缅边境的扩张面积和速率高于中老边境。