耳鲍(Haliotis asinina)核糖体小亚基(18S rRNA)编码基因的克隆与序列分析

采用分子生物学的方法,对南海不同海区的两个地理群体耳鲍(Haliotis asinina)18S rRNA基因全长进行了克隆和序列分析,并将耳鲍18S rRNA基因的序列与NCBI数据库中已收录鲍的18SrRNA基因进行了比较。结果发现,南海耳鲍核糖体18S rRNA基因与耳鲍H.asinina isolate H11核糖体18S rRNA基因序列的相同率高达98%;同一地理群体内耳鲍核糖体18S rRNA基因序列完全一致;不同地理群体间耳鲍核糖体18S rRNA基因在碱基组成上的相似率为99%,仅在某些位点处发生了碱基替换,即腺嘌呤(T)被鸟嘌呤(G)替换;同时,将这两个不同群体中耳鲍的18S rRNA基因与泰国耳鲍18S rRNA基因序列进行比较分析发现,它们之间也只是发生了碱基替换。     

刀额新对虾染色体核型及细胞核DNA含量

以刀额新对虾胚胎、无节幼体、卵巢、精巢等为材料 ,空气干燥法制备染色体 ,并初步进行核型分析。结果表明 ,刀额新对虾的染色体数目为 2n=78,n =39,核型为 2n=78=40M 1 0SM 1 4ST 1 4T。以刀额新对虾血淋巴、卵巢、肌肉、鳃为材料 ,以鸡血细胞为DNA标准 (2 50pg/ 2c) ,使用PartecCCAⅡ型流式细胞仪测定了刀额新对虾细胞的基因组DNA含量 ,其值为鸡血细胞的 1 75倍 ,绝对含量为 4 375pg/ 2c。     

光生物反应器中光衰减特征与螺旋藻生长动力学研究

分析了光照强度在光生物反应器中的分布特征，结果表明，当光波长及光传播的路径确定时，光生物反应器中光衰减特征主要受培养物生物量浓度的影响，由回归的模型对实验数据的拟合可分析光衰减特征与培养物生物量浓度的相关性，为光生物反应器中平均光照强度的确定奠定基础。在光生物反应器中，当营养底物和环境温度不是螺旋藻生长限制因子时，通过平均光照强度对螺旋藻比生长速率的影响分析，结果表明，在实验条件下，螺旋藻比生长速率与平均光照强度的动力学模型可用Aiba光生长抑制方程描述，光亲和系数Ks为238.29umol/(m^2.s)，光抑制系数Ki为0．00493s.m^2/umol，光生物反应器中螺旋藻生长的饱和光照强度出现在190－272umol/(m^2.s)的范围内。     

Long-term Sensor Drift Found in Recovered Argo Profiling Floats

We recovered three Argo profiling floats after 2 to 2.5 years of operation, and recalibrated their temperature, conductivity, and pressure sensors. The results demonstrate that these floats exhibited a significant drift in salinity of −0.0074 to −0.0125, primarily due to the conductivity sensor drift. Combined with the recalibration result for another previously recovered float, the indication is that the negative salinity drift increases nearly in proportion to the operating period of floats. The increasing rate is −0.0041 (±0.0015) year⁻¹, which yields a salinity drift of −0.016 (±0.006) for the expected float lifetime of four years. The present result suggests that reducing the float surfacing time would improve the accuracy of the salinity measurements.     

鱿鱼精核蛋白提取物的保健功能研究

以鱿鱼精巢组织中提取的核蛋白为主要成分制成精核蛋白提取物 ,利用功能学评价实验方法对提取物进行抗疲劳、抗氧化以及免疫调节实验。结果表明 :( 1 )该提取物能明显延长小鼠爬杆和负重游泳时间 ,减少小鼠运动时肝糖元的消耗 ,降低运动后血乳酸的水平 ;( 2 )该提取物能降低血中过氧化脂质降解产物丙二醛 ( MDA)含量、增强血中超氧化物歧化酶( SOD)活力 ;提高小鼠 NK细胞活性、巨噬细胞吞噬百分比和吞噬指数及细胞免疫水平。因而 ,该提取物有抗疲劳、抗氧化以及免疫调节作用     

基于EMD与神经网络的机械故障诊断技术

经验模式分解 (EMD)是分析非线性、非平稳信号的有力工具 ,它将信号分解为突出了原信号的不同时间尺度的局部特征信息的内在模函数 (IMF)分量。本文通过将各 IMF分量输入到 BP网络中进行训练学习和故障诊断 ,比直接输入原信号可以提高 BP网络对故障诊断的准确率 ,而且减少了训练时间。     

Simple determination of dimethylsulfide (DMS) and dimethylsulfoniopropionate (DMSP) using solid-phase microextraction and gas chromatography-mass spectrometry

Solid-phase microextraction (SPME) is a simple, sensitive and less destructive method for the determination of dimethylsulfide (DMS) in seawater. Combined with detection by gas chromatography-mass spectrometry (GC-MS), the method had sufficient sensitivity (minimum detectable concentration of DMS was 0.05 nM), and practical levels of reproducibility (relative standard deviation ≤7%) and linearity (r ² > 0.995) over a wide concentration range (0.5 to 910 nM). The protocol developed was applied to a Sagami Bay water sample to determine concentrations of DMS and DMSP, and in situ DMSP-lyase activity.     

Eddy Shedding from the Kuroshio Bend at Luzon Strait

TOPEX/POSEDIENT-ERS satellite altimeter data along with the mean state from the Parallel Ocean Climate Model result have been used to investigate the variation of Kuroshio intrusion and eddy shedding at Luzon Strait during 1992–2001. The Kuroshio penetrates into the South China Sea and forms a bend. The Kuroshio bend varies with time, periodically shedding anticyclonic eddies. Criteria of eddy shedding are identified: 1) When the shedding event occurs, there are usually two centers of high Sea Surface Height (SSH) together with negative geostrophic vorticity in the Kuroshio Bend (KB) area. 2) Between the two centers of high SSH there usually exists positive geostrophic vorticity. These criteria have been used to determine the eddy shedding times and locations. The most frequent eddy shedding intervals are 70, 80 and 90 days. In both the winter and summer monsoon period, the most frequent locations are 119.5°E and 120°E, which means that the seasonal variation of eddy shedding location is unclear.     

Evidence for sinistral strike-slip deformation in The Solomon Island arc

During the SOPACMAPS 2 crusie carried out by IFREMER (Institut Français de Recherche pour l'Exploitation de la Mer) and ORSTOM (Institut Français de Recherche Scientifique pour le dévelopement en Coopération) on theR/V L'Atalante, in the Central Solomon Arc area, multibeam bathymetric and imagery data and single-channel seismic reflection profiles were collected from an area of about 3500 km², to evaluate regional tectonics. Structural data geophysical profiles interpretation provide evidence for left-lateral transtensional tectonics on the southern edge of the Central Solomon Trough. This transtensional deformation is represented by faulting, block tilting, and rhombohedral deformation. The regional geology and the analysis of the sedimentary cover allow us to demonstrate that this tectonic occurred in two different phases during Oligocene to Miocene and Pliocene to Pleistocene times.     

高效液相色谱法测量青海湖浮游植物色素浓度

2003年8月，我们利用高效液相色谱法(HPLC)对浮游植物色素进行分类定量分析。结果表明青海湖的叶绿素类以叶绿素a和b为主，叶绿素a浓度最高；光合作用类胡萝卜素种类极少，浓度很低；光保护色素种类较多，浓度较高，其中以Zea，Diadino，β．β-Car和Viola为主。