Tetraploid induced by physical and chemical methods in Jinjiang oyster(Crassostrea rivularis)

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双斑东方鲀人工繁殖及育苗技术

本文报道了福建省水产研究所 2 0 0 1年双斑东方人工繁殖及育苗过程与结果 .在春季当海水水温达到 1 6℃以上时 ,用DOM、LRH A3或HCG进行催产 ,混和或单一使用 ,剂量视亲鱼性腺成熟度而定 .受精卵在水温 1 8.0～ 2 2 .4℃、盐度 2 2 .1～2 8.6条件下 ,经 1 1 7～ 1 56h孵化 .用 2 0 5万尾开口仔鱼培育出平均全长为 3 7mm ,平均体重为 1 .3 g的鱼苗 52 .3 5万尾 ,成活率 2 5.5%.此外 ,对亲鱼的暂养、催产、受精卵的孵化、仔鱼的前期培育和后期培育等关键环节进行了探讨 .     

不同年度收存的褶皱臂尾轮虫休眠卵的孵化及滞育胚胎“激活”效果的研究

比较了 1 985— 1 998年间由同一褶皱臂尾轮虫 (Brachionusplicatilis)种群在实验室内历年产生并收存的休眠卵的孵化效果 ,并初步探讨了胚胎“激活”处理对孵化的影响。结果表明 ,休眠卵的收存年度或时间对其在一定孵育期内的总孵化率 (THR)和日孵化率 (DHR)有极显著影响 (x2 检验 ,p<0 .0 0 1 ) ,但THR和DHR与储存时间间均无显著的相关性 ,而是呈极大波动。同时发现“激活”作用具有促使孵化高峰提前到来、增强孵化同步性的双重功效 ,从而可明显缩短孵育时间 ,提高孵化速率。但“激活”前后多数卵样的总孵化率无显著变化 (p >0 .0 5 )。详细讨论了可能导致孵化率波动的多种因素 ,例如遗传变异、培养历史、储存及孵化的环境条件等。     

福建海岛潮间带软体动物群落分布特征

福建海岛潮间带软体动物共鉴定248种，隶属于5纲78科161属，应用群落系数，等级聚类分析和极坐标排序研究软体动物落分布，结果表明，软体动物群落可分为3种类型，即河口型群落，近岸港湾群落和开阔海域群落，群落分布与海岛所处地理位置及其生境类型密切相关，盐度和水动力是重要的影响因子。     

大鹏湾潮流数值计算

应用分步杂交方法建立了大鹏湾边界潮流数值模型，并对其进行了模拟计算，重现了该区域潮波及潮流的分布规律，并根据实测资料对其进行了验证，从而可系统地了解这一海区的潮波系统及潮流场等海洋要素的分布情况。     

海上不同高度风速换算关系的研究

本文用海上石油平台上观测的风梯度资料,计算了在不同风况下海面摩擦速度和粗糙高度。结果表明,海面摩擦速度u_*与海上10m高度处风速u_(10)的关系为u_*=0.055u_(10)-0.058。在4—5级风时,海面平均摩擦速度为50cm/s,粗糙度为0.022m,它相当于陆上的平均粗糙高度。在风速为2—3级时,二者与海上通常采用的数据接近。文中最后给出了上述两种风况下海上不同高度的换算系数。     

厦门海洋生物入侵的危害及管理对策

本文系统地分析了厦门海洋生物入侵的成因、现状、危害及治理方法,认为互花米草和海洋动物沙筛贝是主要的入侵物种,其严重的生态、经济和社会危害不可低估.同时明确防止海洋生物入侵的总体目标,进而提出强化管理的系列对策.     

Seasonal variations of alkenones and U37 in the Chesapeake Bay water column

Alkenone unsaturation indices (U^K₃₇ and U^K′₃₇) have long been used as proxies for surface water temperature in the open ocean. Recent studies have suggested that in other marine environments, variables other than temperature may affect both the production of alkenones and the values of the indices. Here, we present the results of a reconnaissance field study in which alkenones were extracted from particulate matter filtered from the water column in Chesapeake Bay during 2000 and 2001. A multivariate analysis shows a strong positive correlation between U^K₃₇ (and U^K′₃₇) values and temperature, and a significant negative correlation between U^K₃₇ (and U^K′₃₇) values and nitrate concentrations. However, temperature and nitrate concentrations also co-vary significantly. The temperature vs. U^K₃₇ relationships (U^K₃₇=0.018 (T)−0.162, R²=0.84, U^K′₃₇=0.013 (T)−0.04, R²=0.80) have lower slopes than the open-ocean equations of Prahl et al. [1988. Further evaluation of long-chain alkenones as indicators of paleoceanographic conditions. Geochimica et Cosmochimica Acta 52, 2303–2310] and Müller et al. [1998. Calibration of the alkenone paleotemperature index U^K′₃₇ based on core-tops from the eastern South Atlantic and the global ocean (60°N–60°S). Geochimica et Cosmochimica Acta 62, 1757–1772], but are similar to the relationships found in controlled studies with elevated nutrient levels and higher nitrate:phosphate (N:P) ratios. This implies that high nutrient levels in Chesapeake Bay have either lowered the U^K₃₇ vs. temperature slope, or nutrient levels are the main controller of the U^K₃₇ index. In addition, particularly high abundances (>5% of total C₃₇ alkenones) of the tetra-unsaturated ketone, C_37:4, were found when water temperatures reached 25 °C or higher, thus posing further questions about the controls on alkenone production as well as the biochemical roles of alkenones.     

A new form of generalized Boussinesq equations for varying water depth

A new set of equations of motion for wave propagation in water with varying depth is derived in this study. The equations expressed by the velocity potentials and the wave surface elevations include first-order non-linearity of waves and have the same dispersion characteristic to the extended Boussinesq equations. Compared to the extended Boussinesq equations, the equations have only two unknown scalars and do not contain spatial derivatives with an order higher than 2. The wave equations are solved by a finite element method. Fourth-order predictor–corrector method is applied in the time integration and a damping layer is applied at the open boundary for absorbing the outgoing waves. The model is applied to several examples of wave propagation in variable water depth. The computational results are compared with experimental data and other numerical results available in literature. The comparison demonstrates that the new form of the equations is capable of calculating wave transformation from relative deep water to shallow water.     

Photoinduced toxicity single and binary mixtures of four polycyclic aromatic hydrocarbons to the marine diatom Skeletonema costatum

Diatom Skeletonema costatum Cleve is one of the main predominant phytoplankton species in the Changjiang Estuary in China. In order to provide some basic information for future assessment of the potential risk on phytoplankton communities in this estuary caused by polycyclic aromatic hydrocarbons(PAHs), this alga was selected as a representative to investigate the photoinduced toxicity of PAHs, in single and mixture. Four PAHs including three-ring phenanthrene and anthracene, four-ring fluoranthene and pyrene were tested in the laboratory. The single toxicity of each PAH on this microalga was compared with and without the simulated solar UV radiation. The results showed that this microalga was sensitive to PAH's photoinduced toxicity. Ratios of the 72 h median effect concentration obtained for fluorescent and UV light tests were about 8.4 for phenanthrene, 13.0 for anthracene, 6.5 for fluoranthene, and 5.7 for pyrene, indicating that UV light enhanced the PAH toxicity to this alga significantly. Under the fluorescent radiation (lacking UV), the dose-response curves based on chemical concentrations revealed that the order of toxic strength was fluoranthene greater than pyrene greater than anthracene greater than phenanthrene; while under the UV radiation (476 μW/cm2 for UVA, 6.5 μW/cm2 for UVB) it became fluoranthene approximately equaling anthracene greater than pyrene greater than phenanthrene, indicating that the UV light also changed its relative toxicity to this alga. The photoinduced toxicity of PAHs to the marine diatom S. costatum might be a synergistic effect of photosensitization reactions (e.g., generation of single-state oxygen) and photomodification (photooxidation and/or photolysis).The combined effects of six binary mixtures on the marine diatom S. costatum were investigated using the additive-index method. Four binary-mixtures (phenanthrene plus anthracene; phenanthrene plus pyrene; anthracene plus fluoranthene; anthracene plus pyrene) were found to be synergistic joint action mode, while two binary-mixtures (phenanthrene plus fluoranthene; fluoranthene plus pyrene) displayed antagonist, revealing a complex pattern of possible interactions. The combined action mode of PAHs might be related to various factors such as the mixture compounds, mixture ratios and test conditions, etc.