Expression of the Phycoerythrin Gene of Gracilaria lemaneiformis (Rhodophyta) in E. coli and Evaluation of the Bioactivity of Recombinant PE

Phycoerythrin (PE) is one of the most important proteins involved in light capturing during photosynthesis in red algae. Its potential biological activities had gained wide concerns. In the present study, tumor cytotoxic and hydroxyl radical assay were preformed to detect the bioactivity of recombinant PE. Recombinant plasmids pGEX-PE and pBGL were transformed into E.coli BL21 to make two recombinant strains BEX (pGEX-PE) and BGL (pBGL). PE expressing in BEX (pGEX-PE) was validated by SDS-PAGE and Western blotting analysis. SDS-PAGE analysis indicated that the PE-GST fusion protein was mostly inclusion bod- ies. Specific expression of PE was confirmed by Western blotting analysis. The recombinant E. coli BEX (pGEX-PE) cells were col- lected and sonicated. The supernatants were reserved for the tumor cytotoxic experiments. The result of tumor cytotoxic assay indi- cated that the supernatants containing PE had the activity of inhibiting the growth of Hela cells and with the increase of protein con- centration, the inhibiting rate increased from 37.31% to 63.26%, which showed significant difference from the control. Hydroxyl radical scavenging effect was tested with supernatants of BEX (pGEX-PE) and BGL (pBGL) cell lysates treated with sonication and heating. For the sonication samples, the scavenging rates of the supernatants of BEX (pGEX-PE) and BGL (pBGL) cell lysates were significantly higher than the negative control BL21(pGEX-4T) (P<0.02), and the scavenging rates increased slowly following the increase of the protein content. For the heating samples, except for the 0.2 mg mL-1 BGL (pBGL) products, the scavenging effects of the supernatants of BEX (pGEX-PE) and BGL (pBGL) cell lysates were stronger than that of negative control BL21(pGEX-4T). However, the effect intensity was not positively correlated with the increase of the protein concentration. Though a partially de- creased hydroxyl radical scavenging activity was led by heating, the biological activity was still retained and conspicuous. This re- search showed that phycoerythrin protein expressing in E. coli has the potential medical and sanitarian value.     

高速远程崩滑动力学的研究现状及发展趋势

高速远程崩滑动力学机理的研究,一直是国内外工程地质与山地灾害领域崩塌、滑坡灾害研究的前沿热点问题。对这一研究领域,最近几十年来所取得的研究成果、研究现状及发展趋势进行了述评。厘定了高速远程崩滑的定义;详细地阐述了国内外众多典型重大高速远程崩滑灾害事件的几何学、运动学特征;概述了迄今为止所提出的高速远程崩滑形成的各种动力学机理;分析与讨论了高速远程崩滑动力学机理研究的发展趋势与动向;提出了今后进一步研究的总体思路、目标与应重点解决的关键问题。     

不同鲜重秋茄胚轴形态及其对幼苗生长的影响

为了探讨不同鲜重秋茄胚轴的表型性状和营养成分含量差异,及其对幼苗生长的影响,确定浙江省秋茄胚轴质量标准,提高造林质量,本研究采集4.0～5.0g、5.0～6.0g、6.0～7.0g、7.0～8.0g、8.0～9.0g、9.0～10.0g共6组不同鲜重的秋茄胚轴,测定其表型性状、营养成分含量和幼苗生长性状。结果表明,胚轴长度、横径、顶径随着胚轴鲜重的增加而显著增加。不同鲜重等级胚轴间的氮(N)、磷(P)、钾(K)和有机碳百分含量差异不显著,但随着秋茄胚轴鲜重增加,淀粉百分含量显著减小。单根胚轴的营养成分总含量随胚轴鲜重的增加而显著增加。胚轴的C:N、N:P和C:P值在鲜重7.0～8.0g等级时均显著高于其他鲜重等级。幼苗生长高、基径、叶片数及生物量随胚轴鲜重的增加而显著增加,但在5.0～6.0g、6.0～7.0g和7.0～8.0g等级间幼苗茎干重、叶干重、净生物量和总生物量差异不显著。胚轴表型性状与幼苗生长指标之间的相关性均呈显著水平(P<0.05),除胚轴P总含量与生长高之间的相关性不显著外, N、K、Na、有机碳、淀粉总含量与幼苗生长指标之间均呈显著正相关关系(P<0.0...     

湘中南二叠系孤峰组硅质岩的成因属性及其地球动力学指示意义

华南二叠系底部的硅质岩及同一层位对应于华南重要的地幔强活动期和成矿期，有非常重要的动力学指示意义。研究表明，孤峰组剖面的岩性可划分为三段：泥页岩段、厚层状硅质岩段和薄层状硅质岩段。沿剖面自下而上，Al2O3、K2O和TiO2含量逐渐降低，MnO、TFe等组分逐渐升高；稀土元素显示出一定的铈负异常，∑REE偏低，且向上∑REE逐渐减少而HREE／LREE逐渐增高；一些热水沉积诊断性微量元素如As、Sb、Hg等也显示出一定程度的异常。这些特征均表明硅质岩的成岩过程中包含有正常沉积和热水沉积的共同作用，且自下而上热水沉积作用逐渐增强的趋势。这种成岩作用的演化特征，代表了下扬子地台拉张裂陷盆地对晚古生代伸展背景的沉积响应，对于探讨裂谷盆地阶段性发育的动力学特征有一定的指示意义。     

吉林省近代火山的特征及成因讨论

吉林省近代火山分布广泛,数量较多,有近10个火山群,数百座火山锥,均是新近纪中新世至全新世火山活动的产物。火山岩石为碱性（含钾高）玄武岩及其相同成分的火山碎屑岩,并含幔源包体。喷发类型从溢流式至猛烈式,其次尚有一种特殊类型——侵出式。火山成因受板内大陆深大断裂的控制,并与大洋板块自中生代以来向西俯冲的运动影响有关。     

长江口斜压诊断模式三维流场数值模拟

建立了σ坐标系下长江口斜压诊断模式三维流场数学模型,采用k-kl二方程紊流闭合模型求解垂向涡粘系数,计算域内恒定、非均匀盐度场反映了计算域密度斜压效应。验证结果表明,斜压诊断模式的模拟成果较切合实际。模型成功复演了长江口水域由往复流向旋转流过渡的流场特性;潮波从四条汊道传入后在分汊口附近相遇形成多个会潮点,其位置随着径流的大小、潮汐的强弱、汊道的形态等因素而变化。     

珠江口盆地恩平凹陷油气充注期次及时间

应用储层流体包裹技术研究了恩平凹陷油气的成藏规律.结果表明,该区油气主要有两期充注,第一期充注时间较早(20 Ma),以油为主,原油主要来自深部文昌组中深湖相烃源岩,大量油气见于浅层储层;第二期充注时间较晚(5 Ma以后),以天然气为主,天然气主要源自文昌组烃源岩,油气主要分布在深层储集层中.研究认为第一期油气充注与盖层形成时间匹配不好,可能是浅层勘探失利的主要原因之一,因此勘探方向应以深层储层(文昌组)为主,寻找深层早期构造更为重要,是下一步勘探的重要领域.