油罐区砖砌防火堤的抗震验算

介绍了油罐区砖砌防火堤的抗震验算基本原理和方法,并按照验算方法实现其通用化计算。防火堤主要受到4种荷载作用,即自重惯性力及其引起的弯矩、堤内静液压力及其引起的弯矩、堤内动液压力及其引起的弯矩、自重引起的垂直力。按不同烈度要求计算得到4种荷载作用校核堤的截面抗力和地基强度。通过两个算例检验该方法在抗震鉴定中的应用。两个防火堤典型断面的截面强度、抗滑动及抗倾覆稳定性、地基强度验算结果分别符合抗震设防7度和8度要求。防火堤作为备用体系,其抗震验算程序的通用性具有实用意义。     

Expression of the Phycoerythrin Gene of Gracilaria lemaneiformis (Rhodophyta) in E. coli and Evaluation of the Bioactivity of Recombinant PE

Phycoerythrin (PE) is one of the most important proteins involved in light capturing during photosynthesis in red algae. Its potential biological activities had gained wide concerns. In the present study, tumor cytotoxic and hydroxyl radical assay were preformed to detect the bioactivity of recombinant PE. Recombinant plasmids pGEX-PE and pBGL were transformed into E.coli BL21 to make two recombinant strains BEX (pGEX-PE) and BGL (pBGL). PE expressing in BEX (pGEX-PE) was validated by SDS-PAGE and Western blotting analysis. SDS-PAGE analysis indicated that the PE-GST fusion protein was mostly inclusion bod- ies. Specific expression of PE was confirmed by Western blotting analysis. The recombinant E. coli BEX (pGEX-PE) cells were col- lected and sonicated. The supernatants were reserved for the tumor cytotoxic experiments. The result of tumor cytotoxic assay indi- cated that the supernatants containing PE had the activity of inhibiting the growth of Hela cells and with the increase of protein con- centration, the inhibiting rate increased from 37.31% to 63.26%, which showed significant difference from the control. Hydroxyl radical scavenging effect was tested with supernatants of BEX (pGEX-PE) and BGL (pBGL) cell lysates treated with sonication and heating. For the sonication samples, the scavenging rates of the supernatants of BEX (pGEX-PE) and BGL (pBGL) cell lysates were significantly higher than the negative control BL21(pGEX-4T) (P<0.02), and the scavenging rates increased slowly following the increase of the protein content. For the heating samples, except for the 0.2 mg mL-1 BGL (pBGL) products, the scavenging effects of the supernatants of BEX (pGEX-PE) and BGL (pBGL) cell lysates were stronger than that of negative control BL21(pGEX-4T). However, the effect intensity was not positively correlated with the increase of the protein concentration. Though a partially de- creased hydroxyl radical scavenging activity was led by heating, the biological activity was still retained and conspicuous. This re- search showed that phycoerythrin protein expressing in E. coli has the potential medical and sanitarian value.     

基于GPS/CDMA集成的移动定位与服务技术

随着空间信息技术的发展,移动定位服务需要更高的定位精度。人们的活动范围越来越不确定,这给移动通信带来了无限商机和挑战,在此领域,日本处于世界领先地位,国内主要有Nokia、Ericsson、Motorola等。提出了一种基于GPS／CDMA集成的移动定位与服务技术,并分析了其中关键技术和实施的可行性。     

我们是怎样预报松潘7.2级地震的——纪念松潘7.2级地震30周年

回顾了1976年8月16日松潘7.2级地震中期、短期与临震预报,以及震后趋势判断的过程,简述了当年预报所依据的各类前兆异常。     

冀南綦村斑状石英二长岩角闪石环带结构及其地质意义

为阐释岩浆两阶段演化过程及其与铁成矿的关系,对邯邢铁矿区斑状石英二长岩中角闪石斑晶环带结构进行了成因矿物学研究.通过角闪石温度计、压力计、氧逸度计和湿度计的计算,发现岩浆在14km深处岩浆房内经过多次岩浆混合过程,其后演化的岩浆侵位到1.9 km的深度.岩浆房的温度为935～964℃,氧逸度为△NNO+0.27～△NN...     

南水北调西线工程区地质图三维可视化表达及其意义

传统地质图由于采用正射投影的方法表达地质现象,只能表达地质现象在二维欧氏空间的定位,很难表达地质现象的三维空间特征。结合数字高程模型,在地理信息系统的支持下制作的南水北调西线工程区的三维可视化地质图,可更好地判读地质体的空间信息,便于分析地质体的空间展布规律。     

高速远程崩滑动力学的研究现状及发展趋势

高速远程崩滑动力学机理的研究,一直是国内外工程地质与山地灾害领域崩塌、滑坡灾害研究的前沿热点问题。对这一研究领域,最近几十年来所取得的研究成果、研究现状及发展趋势进行了述评。厘定了高速远程崩滑的定义;详细地阐述了国内外众多典型重大高速远程崩滑灾害事件的几何学、运动学特征;概述了迄今为止所提出的高速远程崩滑形成的各种动力学机理;分析与讨论了高速远程崩滑动力学机理研究的发展趋势与动向;提出了今后进一步研究的总体思路、目标与应重点解决的关键问题。     

不同鲜重秋茄胚轴形态及其对幼苗生长的影响

为了探讨不同鲜重秋茄胚轴的表型性状和营养成分含量差异,及其对幼苗生长的影响,确定浙江省秋茄胚轴质量标准,提高造林质量,本研究采集4.0～5.0g、5.0～6.0g、6.0～7.0g、7.0～8.0g、8.0～9.0g、9.0～10.0g共6组不同鲜重的秋茄胚轴,测定其表型性状、营养成分含量和幼苗生长性状。结果表明,胚轴长度、横径、顶径随着胚轴鲜重的增加而显著增加。不同鲜重等级胚轴间的氮(N)、磷(P)、钾(K)和有机碳百分含量差异不显著,但随着秋茄胚轴鲜重增加,淀粉百分含量显著减小。单根胚轴的营养成分总含量随胚轴鲜重的增加而显著增加。胚轴的C:N、N:P和C:P值在鲜重7.0～8.0g等级时均显著高于其他鲜重等级。幼苗生长高、基径、叶片数及生物量随胚轴鲜重的增加而显著增加,但在5.0～6.0g、6.0～7.0g和7.0～8.0g等级间幼苗茎干重、叶干重、净生物量和总生物量差异不显著。胚轴表型性状与幼苗生长指标之间的相关性均呈显著水平(P<0.05),除胚轴P总含量与生长高之间的相关性不显著外, N、K、Na、有机碳、淀粉总含量与幼苗生长指标之间均呈显著正相关关系(P<0.0...     

The Impact of Climatic and Environmental Factors on n-Alkanes Indices in Southwestern Tibetan Plateau

n-Alkanes are widely used in paleoenvironmental reconstructions.However,our understanding of changes in the distribution of n-alkanes with climatic and environmental factors remains unclear in arid/semi-arid regions.We sampled 26 surface sediments from three climatic zones across the southwestern Tibetan Plateau to evaluate the sensitivity of chain length distributions of n-alkanes to climatic and environmental parameters.Our observations demonstrate that average chain length (ACL), proportion of aquatic macrophyte (Paq),carbon preference index (CPI) and ratio of the contents of nC27 and nC31 (nC27/nC31) are all sensitive to hydroclimatic conditions.In contrast to commonly-adopted assumptions,the correlations between these indices and hydrological parameters are not always good,which indicates that the interpretation of n-alkane indices is special on the southwestern Tibetan Plateau.These might be related to the vegetation characteristics and seasonality of biological activity,and need to be considered in paleoclimatic reconstruction.The impact of seasonal precipitation on n-alkanes indices was also evaluated.