基于点云数据的改进四叉树空间索引研究

随着测绘行业的不断发展,三维激光扫描技术已经成为地理信息产业中不可或缺的重要组成部分^[1],然而点云庞大的数据量给处理带来了一定的困难。本文针对运用车载移动测量系统所获取的海量点云数据,采用了一种基于编码改进四叉树索引的点云数据组织处理方法。结果证明该方法提高了创建索引的速度,减少了树的深度以及数据的冗余量,并基于此编码进行最邻近查找,具有可行性和有效性。     

海上孤立混凝土构筑物的爆破拆除

在海洋工程中,采用爆破手段拆除海上孤立混凝土构筑物或清理孤立大块石是一种快速、有效的方法。文中详细介绍了爆破方案的确定、爆破孔网参数的设计、爆破药量的计算以及安全防护措施,对于类似工程的实施具有重要的参考价值。     

6种海洋致病性弧菌36kDa外膜蛋白特性分析

研究本着筛选和制备弧菌共同外膜蛋白亚单位疫苗,用十二烷基肌氨酸钠抽提并结合超速离心的方法提取了鳗弧菌(Vibrio anguillarum)、鱼肠道弧菌(V.ichthyoenteri)、溶藻弧菌(V.alginolyticus)、副溶血弧菌(V.parahaemolyticus)、哈维氏弧菌(V.harveyi)和创伤弧菌(V.vulnificus)6种海洋致病性弧菌的主要外膜蛋白, SDS-PAGE图谱比较发现这6种弧菌都存在36 kDa的外膜蛋白条带.通过电泳洗脱分离纯化这6种弧菌的36 kDa外膜蛋白,并制备了鳗弧菌菌株SJ060621的36 kDa外膜蛋白多抗.ELISA和Western-blot印迹结果显示,仅鳗弧菌SJ060621、S010610-1和哈维氏弧菌的36 kDa外膜蛋白存在免疫交叉反应;双向电泳分析了鳗弧菌SJ060621、S010610-1、哈维氏弧菌和溶藻弧菌的36 kDa外膜蛋白,2-DE图谱显示出鳗弧菌SJ060621和S010610-1均由3种等电点相近的蛋白组成,存在很高的同源性,2株鳗弧菌与溶藻弧菌和哈维氏弧菌在蛋白质组成数量和等电点上都存在较大的差异,结果说明通过主要外膜蛋白的分子量和免疫特性研究,可为筛选不同弧菌共同外膜蛋白的亚单位疫苗提供有利参考.     

牙鲆淋巴囊肿病毒靶器官上病毒结合蛋白的鉴定

利用抗牙鲆淋巴囊肿病毒单克隆抗体的间接免疫荧光检测技术,在患淋巴囊肿病牙鲆的表皮、鳃、胃、肠及肾中发现淋巴囊肿病毒的感染,以此确定为病毒感染的主要靶器官。用NP-40细胞裂解液分别抽提靶器官的总蛋白,利用病毒铺覆蛋白印迹技术(VOPBA)对靶器官上淋巴囊肿病毒结合蛋白进行鉴定,结果显示在胃、肠及肾的组织蛋白中均有1个分子量为27.8 kDa的蛋白与淋巴囊肿病毒特异结合,在鳃和表皮的组织蛋白中分别存在分子量为37.6 kDa和56.0 kDa的蛋白与淋巴囊肿病毒特异结合。推测在淋巴囊肿病毒靶器官上的这3种病毒结合蛋白有可能是病毒的受体蛋白。     

GNSS地面测试环境中关于影响站址选择因素的考虑

卫星导航产品在投入正式运行之前需要在地面上对其进行测试和校准,因此需要在地面建立模拟天上GPS卫星星座的测试环境,并通过修建伪卫星发射塔来发射类GPS卫星信号。测试环境中关于伪卫星发射塔站址选择问题是一个值得关注的硬件建设环节,它直接影响了系统的运行性能,本文通过对五个影响站址选择的因素进行论述,详细地说明了它们各自的影响特征,给出了相应的结论。     

2000国家大地坐标系椭球参数与GRS 80和WGS 84的比较

根据2000国家大地坐标系(CGCS 2000)的定义及其所定义的4个基本椭球常数,推导CGCS 2000椭球的主要几何和物理参数,比较这些参数与GRS 80和WGS 84椭球相应参数之间的差异,给出CGCS 2000椭球与GRS 80及WGS 84椭球定义的正常重力值的差异,并分析在CGCS 2000及WGS 84系下同一点坐标的差异.研究表明:CGCS 2000椭球上的正常重力值与GRS 80,WGS 84椭球上的正常重力值的差值分别约为-143.54×10-8m/s2和0.02×10-8m/s2.同一点在CGCS 2000与GRS 80和WGS 84下经度相同,纬度的最大差值分别为8.26×10-11"(相当于2.5×10-6 mm)和3.6×10-6"(相当于0.11 mm).     

In vivo neutralization assays by monoclonal antibodies against white spot syndrome virus in crayfish(Cambarus proclarkii)

The neutralizing activities of eight monoclonal antibodies （MAbs） against white spot syndrome virus （WSSV） （2D2, 2B2,1D2, 1D5, 1C2, 4A1, 6A4 and 6B4） were analyzed by in vivo experiments. Gills from WSSV-infected shrimp were homogenized and ten-fold serially diluted by PBS, and then incubated with MAbs （hybridoma culture supernatant）, respectively. The mixture of WSSV and MAbs were injected into crayfish （Procambarus clarkii）. After challenge, the death rates of crayfish were counted to determine the neutralizing activities of MAbs. At the same time, the mixture of myeloma culture supernatant and WSSV or PBS was served as positive or negative control, respectively. The results showed that, at each virus dilution, the mean time to death of the crayfish injected with MAb-treated virus was significantly longer than that in the positive control, though they all showed 100% mortality within 25 d, and meanwhile, few crayfish died in the negative control. Among the eight MAbs, 2D2, 2B2, 1D2 and 1D5, especially the former two, delayed the mortality significantly, and 1 C2, 4A1 and 6A4 delayed the mortality as well but not so efficiently, while MAb 6IM was efficient only when the virus concentration increased. The results indicated that the anti-WSSV MAbs can neutralize WSSV in different virus dilutions.     

Molecular identification of scallop planktonic larvae using species-specific microsatellites

The identification of scallop larvae is essential to understand the population structure and community dynamics and to assess the potential environmental impacts caused by scallop larvae released or escaped. However, the larvae identification by morphological characteristics is notoriously difficult, mainly due to the small size （usually being less than 150 μm） and vague morphological characteristics among different scallop species. A simple and accurate molecular method was developed to identify four economically farmed scallop species, the Zhikong scallop Chlamys farreri, the noble scallop C. nobilis, the bay scallop Argopecten irradians and the Yesso scallop Mizuhopecten yessoensis. The tests used the high degree of species-specific microsatellite markers, which was specified by transferability analyses, assessed by reference individuals and evaluated by BLAST searches. The sensitivity test indicated that the species-specific microsatellites were sensitive enough for the detection of 1% -2% larvae in mixed plankton samples. Larvae collected from scallop hatcheries and their effluents and from the artificially controlled crosses were well identified to the species/hybrid level. The results demonstrated that the one-step PCR-based assay was technically simple, inexpensive and robust in identification analyses, and also less sensitive to initial quality of template DNA extracted from the ethanol-preserved samples for several years.     

海南岛西北部莲花山周缘海相地层的区内对比及意义

根据岩性、产状和地貌等特征,对海南岛西北部莲花山周缘海岸出露的前全新世海相地层5个典型剖面进行区内对比,以恢复该套地层的产出序列,并推测海相地层的分布范围。结合海相地层的沉积时代和莲花山周缘海岸的地貌特征,探讨了该海岸带的地貌演化过程:中更新世以前,有河流自东南向西北经德义岭、莲花山入海,海相地层沉积区属于滨岸环境,是该海岸带当时受海水进退作用的主要区域,接受着波浪从北面、河流从南面带来的沉积物;中更新世至今,受雷琼地区火山活动的影响,河流被隆起的火山锥阻挡而截流或改道,海相地层沉积区则被抬升成为陆地,并在全新世海岸侵蚀作用下,形成了现今宽广的海蚀平台,海岸后退平均速率约为10 cm/a。     

南海南部断裂的分形研究

分形几何作为定量描述自然界中复杂非线性现象的强有力工具,将其应用于南海南部断裂体系的研究中,通过数盒子法计算了断裂分布的分维值。研究结果表明这种方法是可行的,断裂分布的分维值能有效地描述断裂的空间分布特征。南海南部断裂体系在标度区间25～250km内具有很好的统计自相似性,全部断裂的分维值为1．6601,北东向断裂的为1．3875,北西向断裂的为1．2693。根据断裂分维等值线,沿西南海盆扩张轴,两侧断裂呈对称展布;北东向的与北西向的在空间上有一定的互补性,这反映了两组断裂在发育过程中的相互制约性。结合南海的构造演化及南海南部油气盆地的分布,初步探讨了断裂分形特征与南海的构造演化及断裂分形特征与油气盆地分布之间的关系。