海马齿浮床对富营养化海湾营养盐削减的模型评价：以东山湾八尺门养殖海域为例

Many coastal seas are severely eutrophic and required to reduce nutrient concentrations to meet a certain water quality standard. We proposed a method for nutrient removal by planting Sesuvium portulacastrum at the water surface using the floating beds in the aquaculture area of the Dongshan Bay as an example, which is an important net-cage culture base in China and where dissolved inorganic nitrogen(DIN) and dissolved inorganic phosphate(DIP) reach 0.75 mg/L and 0.097 mg/L, respectively far exceeding China's Grade IV water quality standards.Numerical simulations were taken using the ecological model, field observations and field plantation experimental results to assess the environmental restoration effects of planting S. portulacastrum at some certain spatial scales. Our field experiments suggested that the herbs can absorb 377 g/m~2 nitrogen and 22.9 g/m~2 phosphorus in eight months with an inserting density of ～60 shoot/m~2. The numerical experiments show that the greater the plantation area is, the more nutrient removal. Plantation in ～12% of the study area could lower nutrients to the required Grade II standards, i.e., 0.2 mg/LDIN≤0.3 mg/L and 0.015 mg/LDIP≤0.03 mg/L.Here the phytoremediation method and results provide helpful references for environmental restoration in other eutrophic seas.     

周期性的或伴有贯穿流的西边界流跨隙流动的迟滞变异过程

使用1.5层准地转约化重力模式研究了周期性的或伴有贯穿流的西边界流跨隙流动的迟滞变异过程。当西边界流变化的周期比罗斯贝波在缺口处调整的时间尺度大得多时,在雷诺数增加和减少过程的霍夫分叉点都发生延迟,从而产生新的雷诺数迟滞区间;并且西边界流流态转变的临界值变化显著;且周期强迫越短,雷诺数迟滞区间越大。当西边界流变化的周期与罗斯贝波在缺口处调整的时间尺度相当时,西边界流在缺口的流态呈无迟滞的周期性变化,且西边界流入侵西海盆的程度随周期减少而变小。此外,当贯穿流的流量大于西边界流的一半时,会显著影响西边界流在缺口处的迟滞变异过程;西边界流向西入侵程度和流态转变发生的临界雷诺值均发生变化,且贯穿流流量越大变化越大。     

胶州湾沉积物中石油降解菌的降解能力及多样性研究

2013年青岛输油管道爆炸,大量石油污染了附近海岸。课题组采集了污染的沉积物样品,以原油为唯一碳源和能源,富集了四个石油降解菌群。生物多样性和群落分析表明,Luteibacter、Parvibaculum 和属于食烷菌科的一个属是降解菌群的主要优势菌,都属于变形菌门。从石油降解菌群中分离筛选,获得了9株具有不同16S rRNA基因序列的降解菌,分别属于8个属。重量法测定降解菌的石油降解率,其中5株的石油降解率大于30%。GC-MS分析结果表明,石油降解菌多倾向于降解烷烃,对多环芳烃的降解能力较差,其中5株细菌的烷烃降解率较大,仅1株菌D2对多环芳烃的降解率较大,其降解率在34.9%到77.5%。通过对高通量数据的分析,表明食烷菌属是菌群A和菌群E的主要降解菌群,其中筛选获得的菌株E4可能是菌群E的一株优势降解菌。本研究所筛选菌株证明了其石油降解潜力,为油污染海滩生物修复提供了菌株资源。     

Isolation, distribution and evaluation of cytotoxic and antioxidant activity of cultivable actinobacteria from the Oman Sea sediments

Screening bioactive natural products from bacteria is a determinative step in the drug discovery programs. The present study aim to isolate actinobacteria from the Oman Sea sediments for determining the effects of different culture media and treatments on the yield of the isolation process, and measure the DPPH radical scavenging and Artemia cytotoxic activity of culture extracts of the actinobacterial isolates. A total of 290 actinobacterial isolates were collected from 14 sediment samples. Heat treatment(40.68%) and M_4 medium(29.31%) exhibited the maximum isolation rates of actinobacteria. Streptomyces isolates were dominantly distributed in all of the investigated stations according to 16 S rRNA gene sequencing. The distribution pattern of Streptomyces followed a depth-dependent frequency trend, whereas the members of rare genera including Micromonospora, Nocardia Actinoplanes, Nocardiopsis, Saccharopolyspora and Crossiella were distributed in deeper stations. Approximately,25% of the examined isolates could scavenge 90% of 10~(–4) mol/L DPPH solutions at 1 250 μg/mL final concentration of their ethyl acetate culture extracts. Furthermore, the most potent extracts could scavenge DPPH radicals with IC50 ranges from 356.8 to 566.4 μg/mL. Brine shrimp cytotoxicity tests showed that 38.88% of the examined culture extracts exhibited LC_(50) lower than 1 000 μg/mL against the Artemia cells. Moreover, the most potent culture extracts exhibited LC_(50) range from 335.4 to 534.4 μg/mL. Phylogenetic analysis by 16 S rRNA gene sequence revealed that the OS 005, OS 263 and OS 157 closely related to Streptomyces djakartensis, Streptomyces olivaceus and Nocardiopsis dassonvillei respectively. These results suggested the widespread distribution of the antioxidant and cytotoxic producing actinobacteria in the Oman Sea sediments, which could be considered as promising candidates for the discovery of microbial bioactive compounds.     

Experimental,Numerical and Simplified Theoretical Model Study for Internal Solitary Wave Load on FPSO with Emphasis on Scale Effect

Scale effect of ISWs loads on Floating Production Storage and Offloading(FPSO) is studied in this paper. The application conditions of KdV, eKdV and MCC ISWs theories are used in the numerical method. The depthaveraged velocities induced by ISWs are used for the velocity-inlet boundary. Three scale ratio numerical models λ=1, 20 and 300 were selected, which the scale ratio is the size ratio of numerical models to the experimental model.The comparisons between the numerical and former experimental results are performed to verify the feasibility of numerical method. The comparisons between the numerical and simplified theoretical results are performed to discuss the applicability of the simplified theoretical model summarized from the load experiments. Firstly, the numerical results of λ=1 numerical model showed a good agreement with former experimental and simplified theoretical results. It is feasible to simulate the ISWs loads on FPSO by the numerical method. Secondly, the comparisons between the results of three scale ratio numerical models and experimental results indicated that the scale ratios have more significant influence on the experimental horizontal forces than the vertical forces. The scale effect of horizontal forces mainly results from the different viscosity effects associated with the model’s dimension.Finally, through the comparisons between the numerical and simplified theoretical results for three scale ratio models, the simplified theoretical model of the pressure difference and friction forces exerted by ISWs on FPSO is applied for large-scale or full-scale FPSO.     

基于高通量测序方法分析荣成月湖潮间带趋磁细菌多样性

荣成月湖是一个典型天然潟湖,潮间带沉积物中存在多种形态趋磁细菌。通过Roche454高通量测序平台,对沉积物样品(B_S)和磁收样品(B_M)进行16SrRNA基因高通量测序,认识荣成潮间带沉积物的细菌群落结构,并了解趋磁细菌多样性及系统进化地位。研究结果表明沉积物样品中主要的细菌类群为δ-变形菌纲,占总细菌数的26.4%,其次是γ-变形菌纲和α-变形菌纲;而磁收样品中细菌多样性和种类明显降低,以α-变形菌纲占绝对优势,相对比例达72.6%。在磁收样品和沉积物样品分别发现了1 612条和186条reads与趋磁细菌相关,分别占细菌总数的5.76%和0.85%,磁收样品中趋磁细菌数是沉积物样品的6.8倍。对两个样品中获得的趋磁细菌序列进行系统进化分析,发现这些序列多数属于变形菌门的α-变形菌纲,以趋磁球菌占绝对优势,少数属于δ-变形菌纲,与多细胞趋磁原核生物亲缘关系最近。海洋趋磁螺菌属仅在B_M样品中检出,趋磁弧菌属在B_M样品优势度高于B_S样品,而多细胞趋磁原核生物和趋磁螺菌属在B_S样品中优势度更高。通过分析样品间的差异OTUs,认为荣成潮间带沉积物中可能存在大量未知的趋磁细菌新类群。研究结果为下一步培养和开发趋磁细菌这一功能菌群,发现趋磁细菌新类群及趋磁细菌生态功能提供了基础资料。     

漠阳江入海口海岸线分维及其机制分析

分维数是反映海岸线复杂程度的客观依据。结合国内外海岸线分形研究进展,本文以漠阳江入海口附近海域为研究区,基于Landsat 8 OLI遥感影像对研究区域提取瞬时水边线,并利用潮汐数据进行校正得到海岸线;利用Fractal Fox2.0计算获得该海岸线的分维数为1.071;通过对研究区的分形机制分析,认为漠阳江附近的断裂构造对海岸起宏观控制作用,从而对海岸线的分维值也起了决定作用。根据断裂体系模型计算得到西侧海岸线的理论分维值为1.089,而对数螺线模型计算得到东侧海岸线的分维值为1.171;实际分维值相对理论值偏小,说明海岸带的沉积与侵蚀影响了分维值。此外,根据本区海岸侵蚀原因的分析,对分维机制进行了探究,初步论证的分维值大小可为海岸的冲淤变化研究提供参考。     

西南大西洋乌拉圭近海双船拖网捕捞调查

为促进我国海洋渔业"走出去",进一步发展远洋捕捞业,文章调查西南大西洋乌拉圭近海双船拖网捕捞的渔具和渔法等,并提出生产建议。研究结果表明:双船拖网的渔具包括渔船、网具、纲索和属具,网身采用加目和减目编缝的方式缝合而成;渔法包括放网准备、放网、曳网、起网和网具养护等环节;针对渔场、渔期和捕捞对象,应把握捕捞产量、成本和航次的关系,并结合主捕对象的生活习性,合理规划生产。     

A method for the extraction of partition lines from long and narrow patches that account for structural features

The extraction of partition lines for long and narrow patches (LN patches) is an important yet difficult problem in the generalization of thematic data. When current methods are used to process polygons with irregular shapes or complex branch convergence zones, the extracted line structural features tend to be inaccurate and topologically erroneous. In this article, we propose an improved partition lines extraction algorithm of constrained Delaunay triangulation to counter these issues. The proposed method aims to maintain consistency between the extracted line structure characteristics and the actual object structure, especially for complex branch convergence zones. First, we describe three types of aggregation patterns (Type A, B, and C aggregation zones) that occur in partition line extractions for LN patches of complex branch convergence zones using Delaunay triangulation. Then, a partition line extraction algorithm that accounts for the direction between the edges of triangles and the distance of nodes in aggregation zones is proposed. Finally, we test our method for a dataset relating to Guizhou Province, China. Compared with the current method that uses quantitative indicators and visualization, the results indicate that our method not only has applicability for simple situations but also is superior for preserving structural features of complex branch convergence zones.