Cloning and phylogenetic analysis of a fatty acid elongase gene from Nannochloropsis oculata CS179

Nannochloropsis oculata CS179, a unicellular marine microalga, is rich in long-chain polyunsaturated fatty acids (LCPUFAs). Elongase and desaturase play a key role in the biosynthesis of PUFAs. A new elongase gene, which encodes 322 amino acids, was identified via RT-PCR and 5′ and 3′ RACE. The sequence of the elongase gene was blast-searched in the NCBI GenBank and showed a similarity to those of the cryptosporidium. But the NJ-tree revealed that the N. oculata CS179 elongase clustered with those of the microalgae Phaeodactylum tricornutum, Ostreococcus tauri and Thalassiosira pseudonana.     

深基坑开挖与支护的有限元模拟

采用弹塑性有限单元法对超大基坑的开挖与支护进行数值模拟,据此分析土体的位移、主应力分布、施工过程中支护结构受力,并比较基坑顶部水平位移的计算值与实测值,两者非常接近,计算结果较为理想,可为工程设计和现场施工提供理论参考和指导.     

海洋毒素大田软海绵酸完全抗原的制备与分析

为了建立大田软海绵酸(Okadaic acid,0A)的免疫学检测方法,利用活泼酯法将OA分子中的羧基与栽体蛋白上的氨基偶联,人工制备OA的免疫抗原(OA-IgG)和检测抗原(OA-BSA),经电泳、动物实验及ELISA法进行了鉴定,完全抗原的偶联成功,为利用杂交瘤技术制备OA的单克隆抗体和海产品OA免疫学检测方法奠定了良好的基础.     

轻型感应耦合数据传输温盐链系统

介绍了轻型感应耦合数据传输温盐链系统功能、组成和设计过程.该系统用于测量不同深度处温盐的动态变化,具有体积小、重量轻、成本低、布放方便、自成系统的特点,可以应用于水质监测浮标,表面漂流浮标等多种小型平台,测量数据可以服务于水产养殖、环境保护和生态治理、气象和海洋监测预报、军事监测等多个领域.     

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机载LiDAR地形特征信息快速提取

为更有效地获取地形特征信息,提出一种机载LiDAR地形特征信息快速提取算法。首先,通过构建二次曲面拟合模型,建立实测LiDAR地形数据与拟合曲面的几何规则;然后,采用LM算法迭代参数寻优,获得最优化结果下的地形拟合参数,计算拟合时间及拟合精度;最后,以地形拟合模型为基础,进行地形特征信息的快速提取。通过机载LiDAR实测数据验证,当最优搜索半径为2 m时,地形曲面的拟合时间仅为0.02 s,RMSE仅为5.09 cm。该算法保证了地形特征信息提取效率和精度,能够有效满足机载LiDAR科学研究和工程应用的技术需求。     

Photosynthetic response of <Emphasis Type="Italic">Scirpus validus</Emphasis> and <Emphasis Type="Italic">Typha orientalis</Emphasis> to elevated temperatures in Dianchi Lake,Southwestern China

Understanding the effects of simulated warming on photosynthetic performance of aquatic plants may provide strong supports for predicting future dynamics of wetland ecosystems in the context of climate change. The plateau wetlands located in Yunnan province are highly sensitive to climate warming due to their high altitude and cold temperature. Here, we conducted a temperaturecontrolled experiment using two temperature manipulations (ambient temperature as the control and 2°C higher than ambient temperature as the warmed treatment) to determine the photosynthetic characteristics of two lakeside dominant species (Scirpus validus Vahl and Typha orientalis C. Presl.) in Dianchi Lake. Net photosynthetic rate (P_n), stomatal conductance (G_s), intercellular CO₂ concentration (C_i), and transpiration rate of S. validus that grew under warmed treatment were all significantly higher than those under the control. G_s and C_i of T. orientalis showed similar patterns as S. validus did. For the response curves of P_n to photosynthetic active radiation (Pn-PAR) and intercellular CO₂ concentration (P_n-C_i), S. validus had higher P_n values under elevated temperatures than the control, while P_n-PAR and P_n-C_i curves of T. orientalis did not separate clearly under two temperature scenarios. Both S. validus and T. orientalis had higher maximum net photosynthetic rate, light saturation point, dark respiration rate, the maximum rate of RuBP carboxylation (V_cmax), maximum electron transport rate driving RuBP regeneration (J_max), the ratio of V_cmax to J_max, triosephosphate utilization, and 1, 5-bishosphate carboxylase ribulose content under warmed treatment than those under the control. This study provides a preliminary step for predicting the future primary production and vegetation dynamics of plateau wetlands in Yunnan province.     

柱撑蛭石吸附去除废水中重金属离子的实验研究

分别利用有机柱化剂十二烷基磺酸钠(SDS)和无机柱化剂聚羟基铝(HA)对蛭石进行柱撑制得十二烷基磺酸钠柱撑蛭石(SDS-V erm icu lite)和聚羟基铝柱撑蛭石(HA-V erm icu lite),并通过XRD、红外光谱、ZETA电位等表征手段对柱撑蛭石进行表征,同时针对柱撑蛭石对Cu2 ,Cd2 ,C r3 3种重金属离子的吸附进行研究,结果表明:吸附去除率受反应时间、重金属离子的初始浓度、pH值等因素的影响,经柱撑后的蛭石对重金属离子吸附的吸附性能比蛭石原矿要强。柱撑蛭石吸附3种重金属离子的动力学吸附过程可用E lov icb方程和双常数方程进行较好的拟合。     

新疆哈密黄山—镜儿泉镍铜成矿系统的地质构造背景

新疆哈密黄山—镜儿泉镍铜成矿系统的地质构造背景处于塔里木板块与准噶尔板块接合部位、塔里木板块东北边缘 ,与镍铜矿化有关的基性、超基性岩分布于混杂堆积之中 ,混杂堆积中的梧桐窝子群基性火山岩主要属洋壳型岛弧火山岩 ,部分属洋壳火山岩 ,吐—哈陆块是准噶尔洋上的陆块 ,黄山—镜儿泉基性—超基性岩并非是它裂开的产物。     

新矿物—彭志忠石的发现及研究

彭志忠石产于湖南安化白钨矿区,是一种富含镁、锡、铝的复杂氧化物。矿物为浅黄褐色、浅黄色,少量无色,晶体呈六方板状。晶体化学式为:(Mg,Zn,Fe,Al)_4(Sn,Fe)_2(Al,□)_(10)O_(22)(OH)_2,空间群P_3ml,a=5.692(5)A,c=13.78(2)A,V=386.7A~3,Z=1。D=4.22(3)g/cm~3,H≥8,一轴正晶,ω=1.802(2),ε=1.814(2)。