Salinivibrio sp.YH4胞外丝氨酸蛋白酶EYHS耐盐性及生物信息学分析

摘要：目的 探讨菌株Salinivibrio sp.YH4分泌的丝氨酸蛋白酶EYHS的耐盐性及结构特征。方法 明胶底物酶谱法分析EYHS的耐盐性。应用生物信息学手段对EYHS及6种耐盐的S8家族丝氨酸蛋白酶结构特征进行分析。结果 EYHS在4 mol/L的NaCl溶液中仍具有活性,属于耐盐蛋白酶。EYHS及6种S8家族丝氨酸蛋白酶分子表面的loop区等无规则卷曲所占比例较高,α-螺旋与β-片层则主要位于酶分子内部。EYHS分子表面酸性氨基酸含量较高,且具有弱疏水内核。多序列比对发现蛋白酶的催化三联体两侧存在高度保守的基序和保守的极性氨基酸及芳香族氨基酸,并存在多个保守的Gly与Ala。同源模建和表面电荷分布显示,α螺旋和β片层围成了蛋白酶的催化腔,EYHS活性中心包含由Asp32、His65与Ser215组成的催化三联体,且催化位点区域表面静电势为负。结论 上述结构特征可能有助于耐盐丝氨酸蛋白酶EYHS在高盐环境下维持其稳定性和适度柔性,并有助于其催化功能的发挥,为深入研究耐盐丝氨酸蛋白酶的高盐环境适应性提供了一定的理论依据。     

潮汐形变资料中地震前兆信息的识别与提取

将小波变换的多分辨率分析引入潮汐形变资料处理中，对近年来云南地区的3次强震前的潮汐形变观测资料进行了处理与分析，发现在地震前2～5个月，震中附近的形变台站都接收到一个频段相同的异常信号，且它们之间是相关的，相关系数达80％以上，由此可以确定信号是由同一个源发出来的。出现在多个台站的相同频段的异常信号很可能就是我们想要得到的地震前兆信息。     

Theory of stability, and regulation and control of ecological system in oasis

Starting with analysis on the evolving course of oasis and the characteristics and evolution of transitional zone between oasis and desert, in consideration of ecological elements including plant stomata resistance, area covered by vegetation, and physical elements including albedo of vegetation and bare soil, atmosphere temperature, and humidity, under the condition of the balance among net radiation flux, latent heat flux, and sensible heat flux, the following are calculated: temperatures of vegetation and bare soil in different conditions, as well as the evapotranspiration rate of ecosystem. Analysis on evapotranspiration rate indicates that it depends on both the climate of environment and the physiological and ecological conditions of plants. On certain conditions, the evapotranspiration rate of transitional zone between oasis and desert (i.e. area covered by vegetation less than 20%), in some parameter domains, appears in bifurcation or multiequilibrium state. Meanwhile, in such area, ecosystem is extremely unstable. Any minor change to the balance will cause either increase or reduction of area covered by vegetation in ecosystem, on the basis of discussion on the emergency of these phenomena. This paper is attempting to propose an effective way of destruction and rebuilt ecosystem in transitional zone. The way is to control the evaporation of plant through selecting anti-drought country plant with big stomata resistance, and modify the roughness of the underlying surface in ecosystem by establishing rational interspace structure of plant community, so as to put the degenerative ecosystem into the natural succession track. This primary theory is being verified through observation and analysis on historical data.     

Multiperiodic semiregular variable stars in the ASAS data base: A pilot study

Based on a search for multi‐periodic variability among the semi‐regular red variable stars in the database of the All Sky Automated Survey (ASAS), a sample of 72 typical examples is presented. Their period analysis was performed using the Discrete Fourier Transform. In 41 stars we identified two significant periods each, simultaneously present, while the remaining 31 cases revealed even three such periods per star. They occur in a range roughly between 50 and 3000 days. Inter‐relationships between these periods were analyzed using the “double period diagram” which compares adjacent periods, and the so‐called “Petersen diagram”, the period ratio vs. the shorter period. In both diagrams we could identify six sequences of accumulation of the period values. For five of these sequences (containing 97 % of all data points) we found an almost perfect coincidence with those of previous studies which were based on very different samples of semiregular red variables. Therefore, existence and locations of these sequences in the diagrams seem to be universal features, which appear in any data set of semi‐regularly variable red giants of the AGB; we conclude that they are caused by different pulsation modes as the typical and consistent properties of similar stellar AGB configurations. Stellar pulsations can be considered as the principal cause of the observed periodic variability of these stars, and not binary, rotation of a spotted surface or other possible reasons suggested in the literature. (© 2014 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)     

无线射频识别双向认证协议及性能分析

针对姚华桢等人提出的射频识别认证协议易遭受读写器ID揭露,中间人攻击和假冒攻击等安全脆弱性,提出一种基于NTRU公钥密码算法的改进双向认证协议,并用GNY逻辑进行了形式化分析.协议不仅具有一次一密的特点,而且在实现双向认证的同时也完成了会话密钥的交换,并将主要的计算放在读写器上,有效降低了服务器的压力;解决了当前协议中存在的位置跟踪,前向安全性等隐私泄漏问题.分析结果表明,该协议不仅能够可抵抗重传,假冒,拒绝服务等多种恶意攻击,而且具有计算复杂度低,通信量小,效率高等特点,适合在低成本标签实现.     

散乱数据插值的HASM方法

本文运用高精度曲面建模(HASM)方法,研究了空间散乱数据插值算法,并以陕西咸阳彬县大佛寺煤矿区的实测数据为案例,使用交叉统计检验方法比较分析HASM方法与地理信息系统(GIS)领域中常用传统插值方法的空间插值结果。结果表明,HASM方法具有较高的精度。本文还分析了传统常用插值方法的一些统计结果,得出一些结论,这些结论对于使用常用传统GIS空间插值方法的研究者有很好的参考作用。     

尼泊尔Ms8.1地震构造背景及对周边地区地震活动趋势的影响

分析印度板块与欧亚板块俯冲带、尼泊尔Ms8.1地震震中区地震构造及地震发震构造,讨论主震对余震触发及余震的时空强特征和地震对周边地区地震活动趋势的影响。初步分析认为,主边界断裂为本次地震的发震构造,属于低角度逆冲断层地震;余震分布范围与震源破裂面积和方式基本一致,具有向东迁移的时空特征;西藏定日Ms5.9、聂拉木Ms5.3地震不属于尼泊尔地震余震,是应力扰动的结果;沿俯冲带向东至喜马拉雅东构造结是大震发生的危险地段。     

Analog simulation of urban construction land supply and demand in Chang-Zhu-Tan Urban Agglomeration based on land intensive use

Journal of Geographical Sciences - Urban land intensive use is an important indicator in harmonizing the relationship between land supply and demand. The system dynamics (SD) can be used to...     

基于DGT和化学提取法研究浙西北地质高背景区土壤镉生物有效性

地质高背景区相较于人类活动引起的土壤镉污染影响范围更广,在区域尺度上对生态系统和人类健康构成危害。土壤镉生物有效性是决定其生物可利用性、生物毒性的关键因素,因此探寻可行的土壤镉生物有效性评价方法对污染农用地安全利用和风险管控具有重要的理论和实际意义。DGT技术、单一提取法、连续提取法和土壤溶液法常用于测定土壤有效镉,但已有研究成果主要基于同种土地利用类型土壤的室内盆栽实验,难以代表自然污染土壤中的复杂情况。为探明各土壤重金属有效态提取技术对地质高背景区不同土地利用类型土壤Cd生物有效性评估效果,本文以浙江西北部土壤Cd高地质背景区水田土壤-水稻籽实和旱地土壤-小白菜样品为研究对象,实验应用DGT技术、单一提取法（0.01mol/L氯化钙提取）、连续提取法（七步连续提取）和土壤溶液法评价土壤中镉生物有效性。结果显示：①研究区水田和旱地土壤Cd平均含量分别为1.07mg/kg和0.73mg/kg,显著高于浙江和全国土壤平均水平,Cd的异常富集主要与浙西北地区广泛分布的黑色岩系有关。②相较于碳酸盐岩区,黑色岩系区土壤中Cd的生物有效组分占比较高,水田和旱地土壤Cd的活动系数（MF）高达59.9%和51.8%,Cd易在土壤-作物系统中发生迁移富集;③植物体内镉含量Cd-P与不同方法测定的有效镉含量均呈显著正相关,但Cd-P与DGT技术测定的有效镉含量相关性优于其他三种方法,水田土壤测得的有效Cd与水稻籽实相关关系：$ {{C}}_{\text{soln}} $>CDGT>$ {{C}}_{{\text{CaCl}}_{\text{2}}} $>$ {{C}}_{{\text{F}}_{\text{1}}\text{+}{\text{F}}_{\text{2}}\text{+}{\text{F}}_{\text{3}}} $,旱地土壤测得的有效Cd与小白菜相关关系：CDGT >$ {{C}}_{{\text{CaCl}}_{\text{2}}} $>$ {{C}}_{{\text{F}}_{\text{1}}\text{+}{\text{F}}_{\text{2}}\text{+}{\text{F}}_{\text{3}}} $>$ {{C}}_{\text{soln}} $。综合比较不同土壤有效Cd测定方法的优缺点,DGT技术可以模拟植物体对Cd的动态吸收过程,更能准确地反映土壤Cd生物有效性,预测作物Cd含量水平,这与已有研究成果一致。此外,本文研究成果表明DGT技术评价土壤Cd生物有效性,不仅适用于人为污染区,也可应用于地质高背景区。