Pyrene induces a reduction in midbrain size and abnormal swimming behavior in early-hatched pufferfish larvae

Spills of heavy oil (HO) have an adverse effect on marine life. We have demonstrated previously that exposure to HO by fertilized eggs of the pufferfish (Takifugu rubripes) induces neural disruption and behavioral abnormality in early-hatched larvae. Here, two kinds of polycyclic aromatic hydrocarbons, pyrene and phenanthrene, were selected to examine their toxic effects on larval behavior of another pufferfish species (T. niphobles). Larvae exposed to pyrene or phenanthrene exhibited no abnormalities in morphology. However, those exposed to pyrene but not phenanthrene swam in an uncoordinated manner, although their swimming distance and speed were normal. The optic tectum, a part of the midbrain, of pyrene-exposed larvae did not grow to full size. Thus, these findings are indicated that pyrene might be a contributor to the behavioral and neuro-developmental toxicity, although there is no indication that it is the only compound participating in the toxicity of the heavy oil mixture.     

Multi-scale modeling of CO2 dispersion leaked from seafloor off the Japanese coast

A numerical simulation was conducted to predict the change of pCO₂ in the ocean caused by CO₂ leaked from an underground aquifer, in which CO₂ is purposefully stored. The target space of the present model was the ocean above the seafloor. The behavior of CO₂ bubbles, their dissolution, and the advection-diffusion of dissolved CO₂ were numerically simulated. Here, two cases for the leakage rate were studied: an extreme case, 94,600 t/y, which assumed that a large fault accidentally connects the CO₂ reservoir and the seafloor; and a reasonable case, 3800 t/y, based on the seepage rate of an existing EOR site. In the extreme case, the calculated increase in ΔpCO₂ experienced by floating organisms was less than 300 ppm, while that for immobile organisms directly over the fault surface periodically exceeded 1000 ppm, if momentarily. In the reasonable case, the calculated ΔpCO₂ and pH were within the range of natural fluctuation.     

Wind- and density-driven circulation in a bay on the Sanriku ria coast,Japan: study of Shizugawa Bay facing the Pacific Ocean

Flow fields in Shizugawa Bay on the Sanriku ria coast, which faces the Pacific Ocean, were investigated using hydrographic observations for the purpose of understanding oceanographic conditions and the process of water exchanges in the bay after the 2011 earthquake off the Pacific coast of Tohoku. In spring to summer, density-driven surface outflow is part of estuarine circulation and is induced by a pressure gradient force under larger longitudinal gradients in density along with lower salinity water in the innermost part of the bay, regardless of wind forcing. In winter to summer, another density-driven current with a thermal structure is induced by a pressure gradient force under the smaller longitudinal density gradients in calm wind conditions. Particularly in winter, Tsugaru Warm Current water can be transported in the surface layer inside the bay. Wind-driven bay-scale circulation with downwind and upwind currents in the surface and deeper layers, respectively, is induced by strong longitudinal wind forcing under the smaller longitudinal density gradients, irrespective of season. Particularly in fall to spring, this circulation can cause the intrusions of oceanic water associated with Oyashio water and Tsugaru Warm Current water in the deeper layer. These results suggest that wind- and density-driven currents can produce the active exchange of water from inside and outside the bay throughout the year.     

Seasonal transition of hydrological processes in a slow‐moving landslide in a snowy region

A comprehensive understanding of seasonal hydrological dynamics is required to describe the influence of pore‐water pressure on the stability of landslides in snowy regions. This study reports on the results of continuous meteorological and hydrological observations over 2 years on a landslide body comprising Neogene sedimentary rocks in northern Japan, where a thick (3–5 m) seasonal snowpack covers the land surface. Monitoring of the volumetric water content in shallow unsaturated zones (<0.8 m depth) and pore‐water pressure in saturated bedrock at depths of 2.0 and 5.2 m revealed clear seasonality in hydrological responses to rainfall and meltwater supply. During snow‐free periods, both the shallow soil moisture and deep pore‐water pressure responded rapidly to intense rainwater infiltration. In contrast, during snowmelt, the deep pore pressure fluctuated in accordance with the daily cycle of meltwater input, without notable changes in shallow moisture conditions. During occasional foehn events that cause intense snow melting in midwinter, meltwater flows preferentially through the layered snowpack, converging to produce a localized water supply at the ground surface. This episodically triggers a significant rise in pore‐water pressure. The seasonal differences in hydrological responses were characterized by a set of newly proposed indices for the magnitude and quickness of increases in the pressure head near the sliding surface. Under snow‐covered conditions, the magnitude of the pressure increase tends to be suppressed, probably owing to a reduction in infiltration caused by a seasonal decrease in the permeability of surface soils, and effective pore‐water drainage through the highly conductive colluvial layer. Deep groundwater flow within bedrock remained in a steady upwelling state, enhanced by increasing moisture in shallow soils under snow cover, reflecting the convergence of subsurface water from surrounding hillslopes.     

Effect of macropores on soil freezing and thawing with infiltration

An understanding of heat transport and water flow in unsaturated soils experiencing freezing and thawing is important when considering hydrological and thermal processes in cold regions. Macropores, such as cracks, roots, and animal holes, provide efficient conduits for enhanced infiltration, resulting in a unique distribution of water content. However, the effects of macropores on soil freezing and thawing with infiltration have not been well studied. A one‐directional soil‐column freezing and thawing experiment was conducted using unsaturated sandy and silt loams with different sizes and numbers of macropores. During freezing, macropores were found to retard the formation of the frozen layer, depending on their size and number. During thawing, water flowed through macropores in the frozen layer and reached the underlying unfrozen soil. However, infiltrated water sometimes refroze in a macropore. The ice started to form at near inner wall of the macropore, grew to the centre, and blocked flow through the macropore. The blockage ice in the macropore could not melt until the frozen layer disappeared. Improving a soil freezing model to consider these macropore effects is required. Copyright © 2016 John Wiley & Sons, Ltd.     

A field experiment of walkaway distributed acoustic sensing vertical seismic profile in a deep and deviated onshore well in Japan using a fibre optic cable deployed inside coiled tubing

A two-dimensional walkaway vertical seismic profiling survey using distributed acoustic sensing was conducted at an onshore site in Japan. The maximum depth and the deviation of the observation well were more than 4,000 m and 81 degrees, respectively. Among the several methods for installing fibre optic cables, we adopted the inside coiled tubing method, in which coiled tubing containing a fibre optic cable is deployed. The signal-to-noise ratio of the raw shot gather was low, possibly due to poor coupling between the fibre optic cable and the subsurface formation resulting from the fibre optic cable deployment method and the existence of considerable tubewave noise. Nevertheless, direct P-wave arrivals, P–P reflections and P–S converted waves exhibited acceptable signal-to-noise ratios after careful optimization of gauge length for distributed acoustic sensing optical processing and the application of carefully parameterized tubewave noise suppression. One of the challenges in current distributed acoustic sensing vertical seismic profile data processing is the separation of P- and S-waves using only one-component measurements. Hence, we applied moveout correction using two-dimensional ray tracing. This process effectively highlights only reflected P-waves, which are used in subsequent subsurface imaging. Comparison with synthetic well seismograms and two-dimensional surface seismic data confirms that the final imaging result has a sufficiently high quality for subsurface monitoring. We acquired distributed acoustic sensing vertical seismic profile data under both flowing conditions and closed conditions, in which the well was shut off and no fluid flow was allowed. The two imaging results are comparable and suggest the possibility of subsurface imaging and time-lapse monitoring using data acquired under flowing conditions. The results of this study suggest that, by adopting the inside coiled tubing method without drilling a new observation well, more affordable distributed acoustic sensing vertical seismic profile monitoring can be achieved in fields such as CO₂ capture and storage and unconventional shale projects, where monitoring costs have to be minimized.     

Evaluating metrics derived from Landsat 8 OLI imagery to map crop cover

Developing techniques are required to generate agricultural land cover maps to monitor agricultural fields. Landsat 8 Operational Land Imager (OLI) offers reflectance data over the visible to shortwave-infrared range. OLI offers several advantages, such as adequate spatial and spectral resolution, and 16 day repeat coverage, furthermore, spectral indices derived from Landsat 8 OLI possess great potential for evaluating the status of vegetation. Additionally, classification algorithms are essential for generating accurate maps. Recently, multi-Grained Cascade Forest, which is also called deep forest, was proposed, and it was shown to give highly competitive performance for classification. However, the ability of this algorithm to generate crop maps with satellite data had not yet been evaluated. In this study, the reflectance at 7 bands and 57 spectral indices calculated from Landsat 8 OLI data were evaluated for its potential for crop type identification.     

Emplacement processes of proto-arc basalt in the Izu–Bonin–Mariana arc system

Ascertaining the emplacement mechanism of oceanic basaltic lavas is important in understanding how ocean floor topography is produced and oceanic plates evolve, particularly during the early stages of crustal development of a supra-subduction zone. A detailed study of the volcanic stratigraphy at International Ocean Discovery Program (IODP) Site U1438 in the Amami Sankaku Basin, west of the Kyushu–Palau Ridge, has revealed the development of lava accretion and ridge topography on the Philippine Sea plate at about 49 Ma. Igneous basement rocks penetrated at Site U1438 are the uppermost 150 m of ~6 km-thick oceanic crust, and comprise, in a downhole direction, sheet flows (12.6 m), lobate sheet flows (61.3 m), pillow lavas (50.7 m), and thin sheet flows (25.3 m). The lowermost sheet flows are intercalated with layers of limestone and epiclastic tuff. Lithofacies analysis reveals that the lowermost sheet flows, limestone, and tuff formed on an axial rise, the pillow lavas were emplaced on a ridge slope, and the lobate sheet flows formed off ridge on an abyssal plain. The lithofacies of the basement basalt corresponds to the upper portions of fast-spreading oceanic crust, suggesting that subduction initiation was associated with intermediate to fast rates of seafloor spreading. The surface sheet flows are olivine–clinopyroxene-phyric basalt and differ from the lower basalt flows that contain phenocrysts of olivine and plagioclase, with or without clinopyroxene. The depleted chrome-spinel composition and olivine–clinopyroxene phenocryst assemblage in the surface sheet flows suggests a slight contribution of water for magma generation not present for the lower basalt flows. Considering the lithological difference between the backarc and forearc oceanic crust in the Izu–Bonin–Mariana arc, with sheet flow dominant in the former, seafloor spreading occurred faster in the later stage of subduction initiation.     

Comparison of stable isotopes,ratios of Cl/Cl and I/I in brine and deep groundwater from the Pacific coastal region and the eastern margin of the Japan Sea

Fifty-three samples, including brines associated with oil and natural gas reservoirs and groundwater samples from deep boreholes, were collected from the Pacific and Japan Sea coastal regions in Japan. The ¹²⁹I/¹²⁷I and ³⁶Cl/Cl ratios, and stable isotopes (δD and δ¹⁸O) are compared to investigate differences related to the geotectonic settings of the two regions. The δD and δ¹⁸O data indicate that brine and groundwater from the Pacific coastal region reflect mixing of meteoric water with connate seawater in the pores of sedimentary rocks. On the other hand, brine and groundwater from the Japan Sea coastal region have been hydrothermally altered. In particular, brines associated with petroleum accumulations at Niigata and Akita showed the same isotopic characteristics as fluids found in the Kuroko deposits of the Green Tuff region in northeastern Japan. There is little difference in the ³⁶Cl/Cl ratios in brine and groundwater from the Pacific and Japan Sea coasts. Most brine and some deep groundwater, except those from the Pleistocene Kazusa Group, have already reached the average secular equilibrium ratio of 9.9 ± 2.7 × 10⁻¹⁵ for their mudstone and sandstone reservoirs. There was no correlation between the ³⁶Cl/Cl ratios and differences in geotectonic setting between the Pacific and the Japan Sea coast. The molar I/Br ratio suggests that the I in all of water samples was of biogenic origin. The average ¹²⁹I/¹²⁷I ratio was 290 ± 130 × 10⁻¹⁵ to 294 ± 105 × 10⁻¹⁵ in both regions, showing no relationship to the different geotectonic settings. The uncontaminated brine and groundwater samples are likely to have retained the original ¹²⁹I/¹²⁷I ratios of marine I released from the old organic matter stored in sedimentary rock.