缀锦蛤亚科(Tapetinae)贝类线粒体DNA序列的系统学分析

采用线粒体16S rRNA和COI序列扩增和测序方法,对隶属于缀锦蛤亚科5属7种动物进行了系统学分析.经Clustal x多重比对和PAuP 4.10 软件分析,得到种间序列的遗传距离并构建了邻接(NJ)系统树.实验数据显示,缀锦蛤亚科为遗传连续的同源类群,其中的浅蛤属(Gomphina)、缀锦蛤属(Tapes)和蛤仔属(Ruditapes)亲缘关系较近,结果与Fischer-Piette等(1971)的缀锦蛤亚科的分类方案基本一致.另外,菲律宾蛤仔R.philippinarum 和杂色蛤仔 R.variegata是蛤仔属在印度洋和太平洋海区的一个组群,尽管两种贝类有明显的重叠分布区和相近的贝壳形态,但二者间的16S rRNA 和COI序列遗传距离均达到了物种间差别.结果支持庄启谦(2001)有关菲律宾蛤仔与杂色蛤仔的形态分类及分布区划分的观点.     

Discussion of “Wave setup and setdown generated by obliquely incident waves” by T.-W. Hsu et al., Coastal Engineering, 53, 865–877, 2006

In the recent paper by Tai-Wen Hsu, John R.-C. Hsu, Wen-Kai Weng, Swun-Kwang Wang, and Shan-Hwei Ou (Coastal Engineering, 53, 865–877, 2006), the authors derived theoretical formulations for calculating the wave setup and setdown induced by obliquely incident waves on a beach. The derivation of an expression for setdown contains errors which would lead to an imbalance in longshore momentum flux outside the surfzone. We correct their derivation and give results in terms of the radiation stress concept in a general case including an oblique wave incidence. We also point out that the correct form of wave setdown is important to describe the zero-net force in the momentum balance outside the surfzone.     

A modified analytical method for the shipboard determination of nanomolar concentrations of orthophosphate in seawater

A method for the determination of nanomolar concentrations of orthophosphate in oligotrophic seawater developed by Liang et al. (2007) has been modified to make it fully feasible for shipboard application and for faster sample throughput with minimized sample volume. The technique is based on the flow injection method with solid phase extraction on a Sep-Pak C18 cartridge and colorimetric detector. The Schlieren effect was minimized by rinsing the cartridge sequentially with 5 mL water and 2 mL 95% ethanol solution. With three micro pumps in parallel, savings of up to 80% in amount of reagents and 25% volume of seawater samples could be achieved in comparison to the previous method. Variation of stopped flow time and sample loading time gave 3 different standard curves, which corresponded to 3 linear ranges within 3.4 and 515 nM. The modified method permits the analysis of samples over a wide range of concentrations, and has been successfully applied to shipboard determination of trace orthophosphate in more than 200 seawater samples during a one-month cruise in the South China Sea. For seawater at concentrations of 20.6, 82.5, 206.2 nM orthophosphate, the relative standard deviations (RSD) (n = 6), determined daily for 6 days on board ship were 4.45%, 4.73% and 6.75%, respectively. Five seawater samples collected in the Station SEATS (South East Asia Time Series Station at 18°N, 116°E) were analyzed using the present method both on board and in a land-based laboratory, as well as with the magnesium hydroxide-induced coprecipitation (MAGIC) method, and showed no significant difference according to the statistical t-test.     

加蓬G4-188区块钻井技术实践认识

加蓬G4-188区块是一个新的海外勘探区块,在钻井过程中遇到了井壁垮塌、气侵、井斜控制困难等诸多难题,影响了钻井施工的速度。针对这些难题,实施了解决G4-188区块钻井施工难点的各项技术措施,为G4-188区块后续钻井施工提供了有益的技术支持。     

正理庄高89-1区块CO2混相驱参数优化数值模拟研究

为了确保高89-1区块CO2混相驱项目的顺利实施,依据该区块的精细油藏描述成果,在优化适于CO2混相驱模型基础上,建立了符合油藏地质特点的三维地质模型。在此基础上对开发指标进行计算,对五个参数（井网、开发方式、压力水平、注入量和采油速度）进行优化,并提出了推荐方案,预测采收率可达23.10%,比弹性开采方式提高15.16%。     

提高机采水平的配套技术及应用

随着濮城油田的持续性高速开发,油田综合含水逐渐上升,油水井井况进一步恶化,作为油田生产主体的机械采油井躺井频繁、管杆泵使用寿命缩短。近几年中原油田采油二厂濮城油田年平均躺井数达560井次之多,导致机械采油井维护费用增加,管杆泵投入增大,严重制约着油田的生产和经营。通过加缓蚀剂在油管内壁形成保护油膜,起到润滑作用;应用特殊抽油杆防腐止磨;应用尼龙扶正器对抽油杆体扶正,避免了钢体与钢体的硬磨;应用旋转井口通过地面人力转动改变油管与抽油杆的偏磨面;配合合理生产参数;各种配套技术的合理应用,使濮城油田躺井减少,避免了频繁作业,增加长寿井,管杆泵投入节约了19.3%。     

JSCORS RTK GPS技术在土方测量中的应用

本文介绍了当今城市GPS应用的发展热点CORS系统的优势,并结合实例介绍了JSCORS RTK技术在某土方测量项目中的应用情况、作业流程,分析了测量时应该注意的问题。     

Comparison of the effects of short-term UVB radiation exposure on phytoplankton photosynthesis in the temperate Changjiang and subtropical Zhujiang estuaries of China

We examined the effect of solar ultraviolet radiation B (UVB, 280–315 nm) on photosynthesis of natural phytoplankton assemblages in the temperate Changjiang River Estuary (CRE) in the East China Sea (ECS), and the subtropical Zhujiang River Estuary (ZRE) in the South China Sea (SCS) from August 2002 to April 2003. The short-term effect of UVB was assessed by exposing samples in quartz tubes/bottles to solar radiation under three treatments: (1) natural sunlight (NS) with UVB (NS-UVB); (2) photosynthetically active radiation (PAR, NS cut off UVB); and (3) NS with additional artificial UVB (NS + A-UVB). Solar UVB apparently inhibited phytoplankton photosynthesis rates. In the temperate CRE-ECS, solar UVB reduced surface phytoplankton photosynthesis by about 28% in August and February, while in the subtropical ZRE-SCS the inhibition was only 22% in September and October. In the CRE-ECS, phytoplankton in the stratified water column displayed stronger UVB inhibition when deeper water samples were exposed to surface UVB. Phytoplankton in the mixed water column did not show strong UVB inhibition, while light shift exposure of deeper phytoplankton in the same water column to surface light produced similar results, indicating that mixing moderates UVB effects. In the ZRE-SCS, surface phytoplankton showed greater photoinhibition in January (sunny). However, in April (cloudy), phytoplankton showed little UVB inhibition. Incubation for a short time without UVB showed a large increase in Chl a at two stations in the ZRE-SCS, but a large decrease at the other station in the presence of UVB. In contrast, in the CRE-ECS, a similar incubation experiment without UVB showed a decrease in Chl a, and small UVB inhibition of Chl a at two stations. Nutrient conditions might have played a role in the difference of UVB inhibition between the two regions as the ZRESCS had relatively high concentrations of all nutrients while PO₄ was only 0.21 μM at one of the CRE-ECS stations. The results suggest that phytoplankton in temperate waters would be more responsive to variation of UVB than ones in subtropical waters.     

Seasonal and interannual variability of oceanic carbon cycling in the western and central tropical-subtropical pacific: A physical-biogeochemical modeling study

The oceanic carbon cycle in the tropical-subtropical Pacific is strongly affected by various physical processes with different temporal and spatial scales, yet the mechanisms that regulate air-sea CO₂ flux are not fully understood due to the paucity of both measurement and modeling. Using a 3-D physical-biogeochemical model, we simulate the partial pressure of CO₂ in surface water (pCO_2sea) and air-sea CO₂ flux in the tropical and subtropical regions from 1990 to 2004. The model reproduces well the observed spatial differences in physical and biogeochemical processes, such as: (1) relatively higher sea surface temperature (SST), and lower dissolved inorganic carbon (DIC) and pCO_2sea in the western than in the central tropical-subtropical Pacific, and (2) predominantly seasonal and interannual variations in the subtropical and tropical Pacific, respectively. Our model results suggest a non-negligible contribution of the wind variability to that of the air-sea CO₂ flux in the central tropical Pacific, but the modeled contribution of 7% is much less than that from a previous modeling study (30%; McKinley et al., 2004). While DIC increases in the entire region SST increases in the subtropical and western tropical Pacific but decreases in the central tropical Pacific from 1990 to 2004. As a result, the interannual pCO_2sea variability is different in different regions. The pCO_2sea temporal variation is found to be primarily controlled by SST and DIC, although the role of salinity and total alkalinity, both of which also control pCO_2sea, need to be elucidated by long-term observations and eddy-permitting models for better estimation of the interannual variability of air-sea CO₂ flux.