EFFECTS OF FISH OIL,DHA OIL AND LECITIHIN IN MICROPARTICULATE DIETS ON STRESS TOLERANCE OF LARVAL GILTHEAD SEABREAM（SPARUS AURATA）

The effects of natural fish oil,DHA oil and soybean lecithin in microparticulate diets on stress tolerance of larval gilthead seabream(Sparus aurata)were investigated after 15 days feeding trials.The tolerance of larval gilthead seabream to various stress factors such as exposure to air(lack of dissolved oxygen),changes in water temperature(low)and salinity(high) were determined.This study showed that microparticulate diet with natural fish oil and soybean lecithin was the most effective for in-creasing the tolerance of larval gilthead seabream to various stresses,and that microparticulate diet with natural fish oil and palmitic acid(16:0)was more effective than microparticulate diet with DHA oil and soybean lecithin.     

Episodic orogeny deduced from coeval sedimentary sequences in the foreland basin and its implication for uplift process of Longmen Mountain, China

Longmen Mountain located at the boundary between the Sichuan Basin and Tibetan Plateau,representing the steepest gradient of any edges of the plateau.Three endmember models of uplift process and mechanism have been proposed,including crustal thickening,crustal flow,and crustal isostatic rebound.Here we use coeval sedimentary sequences in the foreland basin to restraint uplift process and mechanism in the Longmen Mountain.The more than 10,000 m thick Late TriassicQuaternary strata filled in this foreland basin and can be divided into six megasequences that are distinguished as two distinct types.The first type is the wedge-shaped megasequences which are sedimentary response of strong active thrust loading events,characterized by a high rate of subsidence and sediment accumulation,coarsening-upward succession and a dual-sourced sediment supply.This type includes Late Triassic,Late Jurassic to Early Cretaceous and Late Cretaceous to Paleogene megasequences.The second type is the tabular megasequences,characterized by the low rate of subsidence and sediment accumulation,finingupward succession,and a single-sourced sediment supply,which is sedimentary response of isostatic rebound and erosion unloading.This type includes the Early to Middle Jurassic,Middle Cretaceous and Neogene to Quaternary megasequences.Basing on sedimentary,active tectonic,geomorphic evidence,we infer that the direction has been reversed from SSWdirected sinistral strike-slip to NNE-directed dextral strike-slip during 40-3.6 Ma,and since 3.6 Ma,the Longmen Mountain thrust belt belong to times of isostatic rebound and erosional unloading with NNEdirected dextral strike-slip.This suggests that crustal isostatic rebound is a primary driver for uplift and topography of the present Longmen Mountain.The Wenchuan(Ms8.0) earthquake,which ruptured a large thrust fault with NNE-directed dextral strikeslip along the range front,is an active manifestation of this crustal isostatic rebound process with dextral strike-slipping and shortening.This process may be the cause for the Wenchuan Earthquake and the apparent paradox of high relief,little shortening,the relative dearth of historical seismicity in the region.     

CT-1地震计传递函数测试仪的设计

将计算机控制技术应用到甚宽频带地震计的传递函数测试中。讨论了基本的测试方法和原理，阐述了传递函数测试系统的软、硬件设计，给出了测试系统的应用实例。该测试系统具有正弦波自动扫频、阶跃信号法和脉冲信号法等标定方法，满足了甚宽频带地震计的传递函数测试在观测频带、动态范围和测试精度等方面的要求。     

Identification and characterization of endosymbiosis-related immune genes in deep-sea mussels Gigantidas platifrons

Deep-sea mussels of the subfamily Bathymodiolinae are common and numerically dominant species widely distributed in cold seeps and hydrothermal vents.During long-time evolution,deep-sea mussels have evolved to be well adapted to the local environment of cold seeps and hydrothermal vents by various ways,especially by establishing endosymbiosis with chemotrophic bacteria.However,biological processes underlying the establishment and maintenance of symbiosis between host mussels and symbionts are largely unclear.In the present study,Gigantidasplatifrons genes possibly involved in the symbio sis with methane oxidation symbionts were identified and characterized by Lipopoly saccharide(LPS) pull-down and in situ hybridization.Five immune related proteins including Toll-like receptor 2(TLR2),integrin,vacuolar sorting protein(VSP),matrix metalloproteinase 1(MMP1),and leucine-rich repeat(LRR-1) were identified by LPS pull-down assay.These five proteins were all conserved in either molecular sequences or functional domains and known to be key molecules in host immune recognition,phagocyto sis,and ly so some-mediated digestion.Furthermore,in situ hybridization of LRR-1,TLR2 and VSP genes was conducted to investigate their expression patterns in gill tissues of G.platifrons.Consequently,LRR-1,TLR2,and VSP genes were found expressed exclusively in the bacteriocytes of G.platifrons.Therefore,it was suggested that TLR2,integrin,VSP,MMP1,and LRR-1 might be crucial molecules in the symbiosis between G.platifrons and methane oxidation bacteria by participating in symbio sis-related immune processes.     

The hydrogen peroxide impact on larval settlement and metamorphosis of abalone <Emphasis Type="Italic">Haliotis diversicolor supertexta</Emphasis>

Abalone Haliotis diversicolor supertexta is an important economic mollusk. The settlement and metamorphosis are two critical stages during its development period, which has direct influence on abalone survival and production. The influence of reactive oxygen species （hydrogen peroxide） on abalone embryo and juvenile development were examined in this study. Larvae of Haliotis diversicolor supertexta were induced to settlement and metamorphose by exposure to seawater supplemented with hydrogen peroxide. They had the best performance at 800 μmol/L. The concentration of 1 000 μmol/L or higher was toxic to the larvae, as the larvae could settle down only at benthic diatom plates without complete metamorphosis. In addition, H2O2 adding time was critical to the larval performance. 24h after two-day post-fertilization was proved to be the optimal adding time. In this paper, two action mechanisms of hydrogen peroxide are discussed： （1） hydrogen peroxide has direct toxicity to ciliated cells, thus cause apoptosis; （2） hydrogen peroxide, as a product from catecholamines＇ autoxidation process in vivo, can reverse this process to produce neuro-transmitters to induce abalone metamorphosis.     

Molecular phylogenetic analysis of attached Ulvaceae species and free-floating <Emphasis Type="Italic">Enteromorpha</Emphasis> from Qingdao coasts in 2007

Based on the sequence data of the nuclear ribosomal DNA internal transcribed spacer (ITS) 1, 5.8 S, and ITS 2, the molecular phylogeny was analyzed on Ulvaceae species collected from Qingdao coasts in summer of 2007, including 15 attached Ulva and Enteromorpha samples from 10 locations and 10 free-floating Enteromorpha samples from seven locations. The result supported the monophyly of all free-floating Enteromorpha samples, implying the unialgal composition of the free-floating Enteromorpha, and the attached Ulvaceae species from Qingdao coasts were grouped into other five clades, suggesting that they were not the biogeographic origin of the free-floating Enteromorpha in that season. Supported by NSFC (40506030); the Innovative Key Project of the Chinese Academy of Sciences (KZCX2-YW-209); Science & Technology Project of Qingdao City (06-2-2-12-JCH)     

Accessory mineral U–Th–Pb ages and <Superscript>40</Superscript>Ar/<Superscript>39</Superscript>Ar eruption chronology,and their bearing on rhyolitic magma evolution in the Pleistocene Coso volcanic field,California

We determined Ar/Ar eruption ages of eight extrusions from the Pleistocene Coso volcanic field, a long-lived series of small volume rhyolitic domes in eastern California. Combined with ion-microprobe dating of crystal ages of zircon and allanite from these lavas and from granophyre geothermal well cuttings, we were able to track the range of magma-production rates over the past 650 ka at Coso. In ≤230 ka rhyolites we find no evidence of protracted magma residence or recycled zircon (or allanite) from Pleistocene predecessors. A significant subset of zircon in the ~85 ka rhyolites yielded ages between ~100 and 200 Ma, requiring that generation of at least some rhyolites involves material from Mesozoic basement. Similar zircon xenocrysts are found in an ~200 ka granophyre. The new age constraints imply that magma evolution at Coso can occur rapidly as demonstrated by significant changes in rhyolite composition over short time intervals (≤10’s to 100’s ka). In conjunction with radioisotopic age constraints from other young silicic volcanic fields, dating of Coso rhyolites highlights the fact that at least some (and often the more voluminous) rhyolites are produced relatively rapidly, but that many small-volume rhyolites likely represent separation from long-lived mushy magma bodies.     

Dynamics and responses of vegetation to climatic variations in Ziya-Daqing basins,China

Examining the direct and indirect effects of climatic factors on vegetation growth is critical to understand the complex linkage between climate change and vegetation dynamics. Based on the Moderate Resolution Imaging Spectroradiometer(MODIS) Normalized Difference Vegetation Index(NDVI) data and meteorological data(temperature and precipitation) from 2001 to 2012, the trend of vegetation dynamics were examined in the Ziya-Daqing basins, China. The path analysis was used to obtain the information on the relationships among climatic factors and their effects on vegetation growth. It was found that the trends of growing season NDVI were insignificant in most plain dry land, while the upward trends were significant in forest, grass and dry land in Taihang Mountains. According to the path analysis, in 23% of the basins the inter-annual NDVI variation was dominated by the direct effect of precipitation, in 5% by the direct effects of precipitation and temperature, and in less than 1% by the direct effect of temperature or indirect effects of these two climatic factors. It indicated that precipitation significantly affected the vegetation growth in the whole basins, and this effect was not regulated by temperature. Precipitation increase(especially in July, August and September) was favorable to greenness enhancement. Summer temperature rising showed negative effect on plant productivity enhancement, but temperature rise in April was beneficial for the vegetation growth. When April temperature increases by 1℃, the onset date of greenness for natural vegetation will be 2 days in advance. There was a lag-time effect of precipitation or temperature on monthly NDVI for all land use types except grass.