Daily salinity fluctuation alleviates salt stress on seedlings of the mangrove Bruguiera gymnorhiza

Salinity is a vital factor that regulates leaf photosynthesis and growth of mangroves, and it frequently undergoes large seasonal and daily fluctuations creating a range of environments – oligohaline to hyperhaline. Here, we examined the hypotheses that mangroves benefit opportunistically from low salinity resulting from daily fluctuations and as such, mangroves under daily fluctuating salinity (FS) grow better than those under constant salinity (CS) conditions. We compared growth, salt accumulation, gas exchange, and chlorophyll fluorescence of leaves of mangrove Bruguiera gymnorhiza seedlings growing in freshwater (FW), CS (15 practical salinity units, PSU), and daily FS (0–30 PSU, average of 4.8 PSU) conditions. The traits of FS-treated leaves were measured in seedlings under 15 PSU. FS-treated seedlings had greater leaf biomass than those in other treatment groups. Moreover, leaf photosynthetic rate, capacity to regulate photoelectron uptake/transfer, and leaf succulence were significantly higher in FS than in CS treatment. However, leaf water-use efficiency showed the opposite trend. In addition to higher concentrations of Na⁺ and Cl⁻, FS-treated leaves accumulated more Ca²⁺ and K⁺. We concluded that daily FS can enhance water absorption, photosynthesis, and growth of leaves, as well as alter plant biomass allocation patterns, thereby positively affecting B. gymnorhiza. Mangroves that experience daily FS may increase their adaptability by reducing salt build-up and water deficits when their roots are temporally subjected to low salinity or FW and by absorbing sufficient amounts of Na⁺ and Cl⁻ for osmotic adjustment when their roots are subsequently exposed to saline water.     

Individual and combined effects of land use/cover and climate change on Wolf Bay watershed streamflow in southern Alabama

Land use/cover (LULC) and climate change are two main factors affecting watershed hydrology. In this paper, individual and combined impacts of LULC and climate change on hydrologic processes were analysed applying the model Soil and Water Assessment Tool in a coastal Alabama watershed in USA. Temporally and spatially downscaled Global Circulation Model outputs predict a slight increase in precipitation in the study area, which is also projected to experience substantial urban growth in the future. Changes in flow frequency and volume in the 2030s (2016–2040) compared to a baseline period (1984–2008) at daily, monthly and annual time scales were explored. A redistribution of daily streamflow is projected when either climate or LULC change was considered. High flows are predicted to increase, while low flows are expected to decrease. Combined change effect results in a more noticeable and uneven distribution of daily streamflow. Monthly average streamflow and surface runoff are projected to increase in spring and winter, but especially in fall. LULC change does not have a significant effect on monthly average streamflow, but the change affects partitioning of streamflow, causing higher surface runoff and lower baseflow. The combined effect leads to a dramatic increase in monthly average streamflow with a stronger increasing trend in surface runoff and decreasing trend in baseflow. Copyright © 2013 John Wiley & Sons, Ltd.     

全球地面降水月值历史数据集研制

全球降水历史数据是开展气候、水循环等研究的基础。收集整理全球12个数据源降水历史月值资料,通过站号、站名甄别不同数据源中相同台站,对344个通过相关系数、一致率、均值t检验、方差F检验的台站多源资料进行拼接,尽可能多地融合各套数据产品优势,最终形成全球降水历史月值数据集(CMA Global PrecipitationV1.0, CGP)。数据集重点解决当前国际数据产品在东亚地区站点稀少、同时应用多套数据应用门槛较高等问题。数据集收录3.1万个台站共计1.87×107组月降水记录, 4152个台站序列长度达百年。与美国大气海洋局(NOAA)的全球降水数据集(GHCN-M V2.0)对比,CGP新增1万个站点、0.5×107组有效观测记录和1030条百年序列,其中141条百年序列通过多源整合技术获取。CGP的站点和数据量优势主要体现在东亚、东欧、西伯利亚等站点稀疏地区。基于CGP分析的全球降水时空特征与国际同类产品的结果较一致。新增的数据虽然没有改变全球降水分布的总体特征,但对区域性的百年降水变化检测有一定影响。基于CGP的全球降水百年序列结果显示,20世纪前半叶全球降水量偏小,近20年是1900年以来全球降水量最大的时期,各纬度带、各个国家或地区的降水长期变化趋势呈现显著的差异。      

结合投影变换和相位相关的海冰运动监测

为提高对渤海海冰旋转和平移运动的监测精度,提出一种基于投影变换的相位相关跟踪方法。选取连续8景静止水色成像仪(GOCI)图像序列,根据特征图像窗口的投影变换构造辅助函数,通过寻求函数最优解得到旋转角度集合,选择修正相关系数确定最佳旋转角度,同时根据相位信息实现海冰样本间亚像素级别的平移跟踪,消除传统相位相关法中因忽略图像相频特性所造成的匹配误差。实验结果表明,以手动测量的旋转角度为基准,该方法和传统相位相关法的旋转监测均方根误差的最大值/平均值分别为0.59/0.50与1.41/0.94,跟踪速度提高了50.6%;海冰平移运动的速度矢量与辽东湾的现场实测数据及历史资料记录数据基本一致。该方法对渤海海冰旋转和平移运动具有较好的跟踪效果。     

中国沿海多鳞四指马鲅(Eleutheronema rhadinum)与四指马鲅(E. tridactylum)形态与遗传位点差异分析

多鳞四指马鲅(Eleutheronema rhadinum)与四指马鲅(Eleutheronema tridactylum)是四指马鲅属(Eleutheronema)中的名贵经济鱼类,具有良好的养殖开发前景。本文对中国沿海多鳞四指马鲅与四指马鲅群体的26个形态特征值进行了多变量比较分析,提取了7个主成分,累计贡献率达到91.112%,前三个主成分贡献率分别为28.759%、21.467%、15.469%。多鳞四指马鲅与四指马鲅形态差异主要体现在头背部、尾柄部、背腹轴及头尾轴的特征。利用4个形态学参数建立了2群体判别公式,综合判别准确率高达100%,可作为多鳞四指马鲅与四指马鲅物种资源的初步判定方法。另外,利用简化基因组测序开发出多鳞四指马鲅与四指马鲅SNP标记69207个、In Del标记12884个,丰富了四指马鲅属鱼类遗传结构研究数据。本研究为进一步开展四指马鲅数属物种资源评估与保护、选育、功能基因研究提供基础资料。     

Establishment and characterization of a testicular Sertoli cell line from olive flounder <Emphasis Type="Italic">Paralichthys olivaceus</Emphasis>

The culture of Sertoli cells has become an indispensable resource in studying spermatogenesis. A new Sertoli cell line (POSC) that consisted predominantly of fibroblast-like cells was derived from the testis of the olive flounder Paralichthys olivaceus and sub-cultured for 48 passages. Analysis of the mtDNA COI gene partial sequence confirmed that the cell line was from P. olivaceus. Cells were optimally maintained at 25°C in DMEM/F12 medium supplemented with fetal bovine serum, basic fibroblast growth factor, and epidermal growth factor. The growth curve of POSC showed a typical “S” shape. Chromosome analysis revealed that the cell line possessed the normal P. olivaceus diploid karyotype of 2n=48t. POSC expressed dmrt1 but not vasa, which was detected using RT-PCR and sequencing. Immunocytochemistry revealed that the cells exhibited the testicular Sertoli cell marker FasL. Therefore, POSC appeared to consist of testicular Sertoli cells. Bright fluorescent signals were observed after the cells were transfected with pEGFP-N3 plasmid, with the transfection efficiency reaching 10%. This research not only offers an ideal model for further gene expression and regulation studies on P. olivaceus, but also serves as valuable material in studying fish spermatogenesis, Sertoli cell-germ cell interactions, and the mechanism of growth and development of testis.     

Observed characteristics of atmospheric ducts over the South China Sea in autumn

The observed characteristics of lower atmospheric ducts over the South China Sea(SCS) were analyzed based on Global Position Systerm(GPS) radiosonde data collected four times daily during autumn open cruises from 2006 to 2012.Duct occurrence,thickness,and strength over the SCS were about 40%,150-m thick,and 8 M units,respectively,which were larger than during the summer monsoon period.Most ducts occurred at heights 1 500 m and these ducts easily trap electromagnetic wave clusters with wavelengths 2 m.Diurnal variation of the SCS ducts appeared evident.They occurred more often at midnight at higher altitudes(about 1 100 m),with a thickest layer of about 145 m and less frequently during the evening at lower altitudes(about 800 m),with a thinnest layer of about 125 m.Moreover,ducts during the daytime at a mean height of about 900 m,with the greatest strength of about 10 M units.Furthermore,all duct variables observed over the SCS in autumn decreased from north to south.These findings are useful not only in the design of radar and communication systems,but also for evaluating possible effects of anomalous propagation on meteorological radar and military applications.