Establishment and characterization of a testicular Sertoli cell line from olive flounder <Emphasis Type="Italic">Paralichthys olivaceus</Emphasis>

The culture of Sertoli cells has become an indispensable resource in studying spermatogenesis. A new Sertoli cell line (POSC) that consisted predominantly of fibroblast-like cells was derived from the testis of the olive flounder Paralichthys olivaceus and sub-cultured for 48 passages. Analysis of the mtDNA COI gene partial sequence confirmed that the cell line was from P. olivaceus. Cells were optimally maintained at 25°C in DMEM/F12 medium supplemented with fetal bovine serum, basic fibroblast growth factor, and epidermal growth factor. The growth curve of POSC showed a typical “S” shape. Chromosome analysis revealed that the cell line possessed the normal P. olivaceus diploid karyotype of 2n=48t. POSC expressed dmrt1 but not vasa, which was detected using RT-PCR and sequencing. Immunocytochemistry revealed that the cells exhibited the testicular Sertoli cell marker FasL. Therefore, POSC appeared to consist of testicular Sertoli cells. Bright fluorescent signals were observed after the cells were transfected with pEGFP-N3 plasmid, with the transfection efficiency reaching 10%. This research not only offers an ideal model for further gene expression and regulation studies on P. olivaceus, but also serves as valuable material in studying fish spermatogenesis, Sertoli cell-germ cell interactions, and the mechanism of growth and development of testis.     

Observed characteristics of atmospheric ducts over the South China Sea in autumn

The observed characteristics of lower atmospheric ducts over the South China Sea(SCS) were analyzed based on Global Position Systerm(GPS) radiosonde data collected four times daily during autumn open cruises from 2006 to 2012.Duct occurrence,thickness,and strength over the SCS were about 40%,150-m thick,and 8 M units,respectively,which were larger than during the summer monsoon period.Most ducts occurred at heights 1 500 m and these ducts easily trap electromagnetic wave clusters with wavelengths 2 m.Diurnal variation of the SCS ducts appeared evident.They occurred more often at midnight at higher altitudes(about 1 100 m),with a thickest layer of about 145 m and less frequently during the evening at lower altitudes(about 800 m),with a thinnest layer of about 125 m.Moreover,ducts during the daytime at a mean height of about 900 m,with the greatest strength of about 10 M units.Furthermore,all duct variables observed over the SCS in autumn decreased from north to south.These findings are useful not only in the design of radar and communication systems,but also for evaluating possible effects of anomalous propagation on meteorological radar and military applications.     

Application of incomplete similarity theory to the estimation of the mean velocity of debris flows

The mean velocity of debris flow is one of the most important parameters in the design of mitigation structures and in quantitative risk analysis. This study develops a model to predict the mean debris flow velocity observed in the field by applying the incomplete similarity argument. An equation for estimating the Darcy-Weisbach resistance coefficient for debris flows with a volumetric sediment concentration larger than 0.19 is accordingly derived using 128 sets of observation data from nine Chinese gullies, in which both the effect of the volumetric sediment concentration and channel slope on resistance are considered. The derived equation is then verified and compared against five previously published equations by using 61 sets of published observation data from six gullies located in four countries. The applications of the proposed equation are discussed, and the improvements made using the proposed equation are clearly very significant when compared with the previously published equations.     

Stability study on the northern batter of MBC Open Pit using Plaxis 3D

Cracks appeared on the northern batter at Maddingley Brown Coal Open Pit Mine, Victoria, Australia, on 8 November 2013 and a 2-day rainfall event happened 5 days later. This study models the stability of the northern batter considering the effect of the rainfall event and an emergency buttress using finite element method (FEM) encoded in Plaxis 3D. It is found that the batter tended to lead to block sliding after overburden removal. The observed vertical crack would be a combined action of the overburden removal and groundwater flow. The simulated location of cracks agrees well with the actual location, and the simulated heave of the coal seam is in good agreement with the experience in Victoria brown coal open pit mining. The rainfall accelerated the development of the cracks. With the construction of the emergency buttress, the batter became stable that is in good agreement with the monitored data.     

GF-1垂直干旱指数的土壤湿度空间格局分析——以秭归县为例

针对中低分辨率的遥感图像在表征空间异质性较大地区的土壤湿度空间格局存在较大误差问题,该文探讨了基于高分辨率影像在小尺度上分析土壤湿度空间分布及变异规律的可行性。首先利用高分一号WFV数据构建垂直干旱指数来反映秭归县土壤湿度干湿状况,然后进一步分析了该县土壤湿度在水平及垂直方向上的空间格局和分异规律。实验结果表明,野外同步土壤表层水含量测试数据同垂直干旱指数两者表现出较好的相关程度。     

沁水煤田15号煤中氟的赋存状态及其沉积环境分析

氟是煤中有害元素之一,燃煤型氟排放造成的污染已经对人类的健康安全构成威胁。研究煤中氟赋存状态及其沉积环境,对掌握高氟煤分布规律及控制燃煤造成的氟排放具有重要意义。通过对沁水煤田内6座煤矿15号煤样品的分析发现:煤中氟含量与灰分显著相关,与黄铁矿硫、有机硫无相关关系,表明煤中氟主要以无机物形式赋存,且与含硫矿物无关;与钾、镁、硅、铝等元素含量具有显著的相关关系,表明氟有可能的主要赋存形式为金云母、氟金云母等矿物,以及以吸附存在于高岭石、勃姆石、伊蒙混层等黏土矿物中;与钙、磷元素含量无显著相关性,表明氟不以氟磷灰石、含氟羟基磷灰石等矿物大量赋存。此外,通过对沁水煤田15号煤的沉积环境分析发现,弱还原环境、较低盐度、偏酸性、较强的水动力条件有利于该区域煤中氟的富集。     

内蒙古呼包鄂地区近56年来大气环境容量变化特征分析

为探讨内蒙古呼包鄂地区大气对污染物承载能力的变化,科学评价气候变化对大气环境的影响,利用1961—2016年呼包鄂地区8个国家气象站降水量、风速、云量资料,分析了该地区气象条件的变化特征及大气环境容量的时空分布特征,并对大气环境容量与气象要素之间的相关性进行了分析。结果显示:呼包鄂地区大气环境容量1月的最小、4月的最大,春季的>秋季的>夏季的>冬季的,年平均大气环境容量为62.4 t/d/km^2;月、季、年平均大气环境容量呈波动减小趋势,特别是20世纪70年代末期之后减小趋势更为显著;有明显的空间分布特征,由城区向郊区逐渐增大,城郊差异冬季最为明显,夏季差异最小;大气环境容量与降水相关性差,与风速和日最大混合层高度呈显著的正相关关系,特别是与风速相关性更显著,与小风日数呈显著的负相关关系。     

大兴安岭地区古利库金(银)矿床成因探讨

古利库金(银)矿床的形成与早白垩世中晚期火山喷发活动及其所形成的火山穹隆构造有关,矿体定位于火山活动波及范围内,平均成矿温度220℃,平均成矿压力135×105Pa,成矿深度约500～600m,属冰长石-绢云母型浅成低温热液矿床,与国内外同类型矿床具有可比性.     

居住迁移对居民健康的影响研究进展述评

进入城市时代以来,居住环境与居民健康的联系愈发密切,尤其在“流动性"增强的大背景下,居住迁移对居民健康的影响问题受到广泛关注。论文结合知识图谱分析和文献研究法,系统评述居住迁移影响居民健康问题的最新进展。研究发现：① 近15 a来,该领域的文献数量逐年增多,关注度持续上升;文献空间分布主要集中在发达国家,但不同地区的研究内容存在显著差异。② 主要研究对象由“同质性群体”向“异质性群体”转变,从“一般性群体”向“特殊群体”转变;研究方法由数理统计向混合研究、综合应用转变。③ 研究视角经历“人口迁移—个体健康”“居住迁移—环境—个体健康”与“迁居轨迹—环境演变—个体健康发展”等3个演变阶段。④ 近期研究重点有：迁居行为与儿童健康,迁居时空特征与身心健康,住房生涯与身心健康,邻里环境、累积剥夺与健康发展等。结合以上分析,提出如下建议：注重“整合性”分析;关注城市内部迁居行为和多次迁居轨迹对居民健康的影响;扩大迁居引发的“环境”因素(如居住隔离、社会分异、职住空间错位等)研究;更多关注迁居对二代流动人口儿童健康的影响,侧重对非正规居住空间如“城中村”等的研究。