基于GIS的水污染源数据监理与查错系统

城市生活废水、工业污水已经成为城市市政管理的重点内容,查清城市排水管网和水污染源的情况,建立专业的排水管网地理信息系统将有利于城市发展。一直以来,数据质量是GIS建设的关键,通过一个基于GIS的可视化数据监理和查错系统,阐述系统的设计方案、监理流程和功能特点。结合广州市水污染源调查项目,对监理系统在实际工程中的重点、难点应用作了说明。     

基于卫星地面站天线状态预测的任务再规划

集成运行控制系统(Integrated Operation and Control System,IOCS)是极轨气象卫星地面应用系统的重要系统之一,其主要作用之一是规划多卫星地面站接收任务,确保全球资料的完整获取。当卫星地面站某个天线出现异常或不可用时,若仍按原有的任务规划进行数据接收,将直接造成全球资料的不完整,因此需要研究卫星地面站天线状态的预测和相应的任务再规划技术,从而及时发现和处理卫星地面站的天线异常、降低连续多轨数据接收失败风险,最终达到提高极轨气象卫星数据接收成功率的目的。文章设计了基于统计分析的卫星地面站天线状态预测方法,并研究了对后续系统运行改变最小、易实现和结果可复核的任务再规划方法。     

内马铁路一期工程局部不规则二维场地地震反应分析

基于内马铁路一期工程地质资料,利用ABAQUS有限元软件建立某局部不规则二维场地有限元计算模型,利用Python语言进行二次开发,编制二维等效线性化计算程序。开展50年超越概率63%、10%和2%水准下的土层地震反应分析计算,对不规则地形不同位置处的地震动峰值加速度和频谱特性进行对比分析。研究结果表明,不规则地形对地震动特性的影响显著。本文研究结果对内马铁路一期工程地震动参数确定具有指导意义,同时可为跨越不规则地形工程结构抗震设计提供参考。     

Establishment and characterization of a testicular Sertoli cell line from olive flounder <Emphasis Type="Italic">Paralichthys olivaceus</Emphasis>

The culture of Sertoli cells has become an indispensable resource in studying spermatogenesis. A new Sertoli cell line (POSC) that consisted predominantly of fibroblast-like cells was derived from the testis of the olive flounder Paralichthys olivaceus and sub-cultured for 48 passages. Analysis of the mtDNA COI gene partial sequence confirmed that the cell line was from P. olivaceus. Cells were optimally maintained at 25°C in DMEM/F12 medium supplemented with fetal bovine serum, basic fibroblast growth factor, and epidermal growth factor. The growth curve of POSC showed a typical “S” shape. Chromosome analysis revealed that the cell line possessed the normal P. olivaceus diploid karyotype of 2n=48t. POSC expressed dmrt1 but not vasa, which was detected using RT-PCR and sequencing. Immunocytochemistry revealed that the cells exhibited the testicular Sertoli cell marker FasL. Therefore, POSC appeared to consist of testicular Sertoli cells. Bright fluorescent signals were observed after the cells were transfected with pEGFP-N3 plasmid, with the transfection efficiency reaching 10%. This research not only offers an ideal model for further gene expression and regulation studies on P. olivaceus, but also serves as valuable material in studying fish spermatogenesis, Sertoli cell-germ cell interactions, and the mechanism of growth and development of testis.     

广西凤凰山灰岩矿矿山地质环境影响评估及主要治理恢复措施

在了解矿山基本概况和查清矿山地质环境条件的基础上,结合矿山实际情况,从地质灾害、含水层、地形地貌景观和土地资源等方面进行了矿山地质环境影响现状和预测评估。现状评估表明,该矿山主要发育滑坡和危岩,地质灾害中等发育,对土地资源和地貌景观影响较严重;预测评估表明,该矿山引发和遭受的地质灾害主要为崩塌和滑坡,对地下水影响较轻,土地资源和地貌景观影响严重。根据评估结果,提出了植被恢复工程、挡护工程、排水工程等矿山地质环境治理恢复措施,对消除或减弱矿山地质灾害,全面恢复矿山生态环境具有一定指导意义。     

一株H_5N_1亚型禽流感病毒株NA基因的克隆与序列分析

用RT-PCR方法从1个H5N1亚型禽流感病毒分离株A/Chicken/Guangdong/DH/1997扩增NA基因cDNA片段,将其克隆至pMD18-T载体,获得重组质粒pMD-NA,并对其核苷酸序列进行测定和分析。结果表明,该毒株的NA基因长度为1350bp,编码449个氨基酸,与其它H5N1亚型AIV分离株的核苷酸序列同源性为97.0%～99.4%,氨基酸序列同源性为97.7%～99.1%,提示禽流感病毒NA基因保守性较高。NA基因氨基酸序列的聚类分析表明该毒株与来自香港的A/Pheasant/HK/FY155/01和A/Ch/HK/FY150/01两个分离株处于同一进化枝,亲缘关系较近。     

The comparison between allozyme and RAPD makers for the population genetic structure analysis of scallop Chlamys farreri

1INTRODUCTION Since the first use in human genetic research in1966,allozyme has become the most popular genetic marker for population genetics studies for almost all groups of animals and plants(Lewontin and Hubby,1966;Murphy et al.,1996).In the past seve…     

冰芯记录中末次间冰期-冰期旋回气候突变事件的研究进展

冰芯记录中的气候变化是古气候研究中的重要组成部分. 极地、中低纬度和热带地区的冰芯记录表明, 在冰期间冰期旋回大尺度气候变化背景下, 全球经历了一系列数百年至千年时间尺度的快速气候突变事件, 诸如末次间冰期的干冷事件、末次冰期的DansgaardOeschger事件、 Heinrich事件和Younger Dryas事件等, 虽然这些穿插在冰阶中的暖湿气候事件、间冰阶中的干冷气候事件的成因、机制和影响范围还存在明显的不确定性. 主要介绍不同区域冰芯记录中末次间冰期冰期旋回这些气候突变事件发生的时间、过程和机制等的研究进展.     

Direct Observations of Atmospheric Transport and Stratosphere-Troposphere Exchange from High-Precision Carbon Dioxide and Carbon Monoxide Profile Measurements

The balloon-borne Aircore campaign was conducted in Inner Mongolia,China,on June 13 and 14 2018,which detected carbon dioxide(CO_2) and carbon monoxide(CO) profiles from surface to 24 km,showing strong positive and negative correlations between 8 km and 10 km on 13 and 14 June,respectively.Backward trajectories,meteorological analyses,and CO_2 horizontal distributions were combined to interpret this phenomenon.The results indicated that the source region experienced a stratospheric intrusion and exhibited a large horizontal CO_2 gradient;namely,lower CO concentrations corresponded to higher CO_2 concentrations and vice versa.The laminar structure with multiple origins resulted in the highly negative correlation between CO_2 and CO in the upper troposphere on 14 June.The contribution of stratospheric air mass to the upper troposphere and that of tropospheric air mass to the lower stratosphere were 26.7% and24.3%,respectively,based on a mass balance approach.Another interesting phenomenon is that CO_2 and CO concentrations increased substantially at approximately 8 km on 13 June.An analysis based on the backward trajectory implied that the air mass possibly came from anthropogenic sources.The slope of CO_2/CO representing the anthropogenic sources was 87.3 ppm ppm~(-1).In addition,the CO_2 profile showed that there was a large CO_2 gradient of 4 ppm km-1 within the boundary layer on 13 June,and this gradient disappeared on 14 June.     

GF-1垂直干旱指数的土壤湿度空间格局分析——以秭归县为例

针对中低分辨率的遥感图像在表征空间异质性较大地区的土壤湿度空间格局存在较大误差问题,该文探讨了基于高分辨率影像在小尺度上分析土壤湿度空间分布及变异规律的可行性。首先利用高分一号WFV数据构建垂直干旱指数来反映秭归县土壤湿度干湿状况,然后进一步分析了该县土壤湿度在水平及垂直方向上的空间格局和分异规律。实验结果表明,野外同步土壤表层水含量测试数据同垂直干旱指数两者表现出较好的相关程度。