Essential cellular modules for the proliferation of the primitive cell

Bacteria appeared early in the evolution of cellular life on planet Earth, and therefore the universally essential genes or biological pathways found across bacterial domains may represent fundamental genetic or cellular systems used in early life. The essential genes and the minimal gene set required to support bacterial life have recently been experimentally and computationally identified. It is, however,still hard to estimate the ancient genes present in primitive cells compared to the essential genes in contemporary bacteria, because we do not know how ancestral primitive cells lived and proliferated, and therefore cannot directly evaluate the essentiality of the genes in ancestral primitive cells. The cell wall is normally essential for bacterial proliferation and cellular division of walled bacterial cells is normally highly controlled by the essential FtsZ cell division machinery. But, bacteria are capable of reverting to their cell wall deficient ancestral form, called the "L-form". Unlike "normal" cells, L-forms divide by a simple physical mechanism based on the effects of membrane dynamics, suggesting a mode of primitive proliferation before the appearance of the cell wall. In this review, we summarize the experimental and computational investigations of minimal gene sets and discuss the minimal cellular modules required to support the proliferation of primitive cells, based on L-form proliferation.     

Fusulinoidean faunal succession of a Paleo–Tethyan oceanic seamount in the Changning–Menglian Belt, West Yunnan, Southwest China: An overview

Abstract   Fusulinoidean faunal succession from Paleo–Tethyan seamount-type carbonates of the Yutangzhai section in the Central zone of the Changning–Menglian Belt of West Yunnan, Southwest China, is presented for the first time. The Changning–Menglian Belt is one of the orogenic belts that represent the closed main Paleo–Tethys in East Asia. The Yutangzhai section is represented by basalts and overlying carbonates, about 1100 m thick. It exhibits a continuous faunal succession composed of 17 fusulinoidean assemblages ranging from the Serpukhovian (late Mississippian/late Early Carboniferous) to Midian/Capitanian (late Middle Permian/late Guadalupian). No significant faunal break can be recognized in this section. The generic and some specific composition of the Yutangzhai assemblages indicates that the faunal succession is similar to those observed in Tethyan and Panthalassan areas and is of tropical Tethyan type although their generic diversity is definitely lower than those of Paleo–Tethyan shelves, such as South China, Indochina, and Central Asia. Throughout the Yutangzhai section, the carbonate rocks are essentially massive, very pure in composition, and devoid of terrigenous siliciclastic inputs. These lithologic characters are identical to those observed in accreted shallow-marine carbonate successions of seamount origin in Permian and Jurassic accretionary complexes of Japan, for example the Akiyoshi Limestone. This evidence further demonstrates the seamount origin of the basalt–limestone succession in the Central zone of the Changning–Menglian Belt from the viewpoint of lithofacies. In middle Mississippian (middle Early Carboniferous) time, oceanic submarine volcanism that was probably related to hot spot activities formed a number of seamounts and oceanic plateaus. It was active not only in the Panthalassa, but also in the Paleo–Tethys.     

A numerical investigation of eddy-induced chlorophyll bloom in the southeastern tropical Indian Ocean during Indian Ocean Dipole—2006

An eddy-resolving coupled physical–biological model is used to study the effect of cyclonic eddy in enhancing offshore chlorophyll-a (Chl-a) bloom in the southeastern tropical Indian Ocean during boreal summer–fall 2006. The results demonstrate that the offshore Chl-a blooms are markedly coincident with the high eddy kinetic energy. Moreover, the vertical variations in Chl-a, nitrate, temperature, and mixed-layer depth (MLD) strongly imply that the cyclonic eddies induce surface Chl-a bloom through the injection of nutrient-rich water into the upper layer. Interestingly, we found that the surface bloom only occurs when the deep Chl-a maximum is located within the MLD. On the other hand, the response of subsurface Chl-a to the eddy pumping is remarkable, although it is hardly observable at the surface.     

青藏高原季风期降水的日变化

利用1998年夏季GAME－TIBET IOT期间的探空、降水和雷达资料分析了季风期降水和CAPE、LCL热力参量的日变化及其之间的关系。降水的日变化很明显，最大的降水和CAPE的最大值出现在同一时间段。6km和8.5km高度内的大气层结在大部分时间是不稳定的。0400－0800时间内6km以下9km以上的稳定层结阻碍了对流系统的发展，降水的日变化与这些热力参数的日变化有关。同时，利用三维云模式模拟了降水的日变化和水汽及温度对降水的影响，云模式再现了降水和回波强度的最大和最小值，晚上低层的高湿度是影响降水的重要因素。     

Regionality and seasonality of submesoscale and mesoscale turbulence in the North Pacific Ocean

The kinetic energy (KE) seasonality has been revealed by satellite altimeters in many oceanic regions. Question about the mechanisms that trigger this seasonality is still challenging. We address this question through the comparison of two numerical simulations. The first one, with a 1/10° horizontal grid spacing, 54 vertical levels, represents dynamics of physical scales larger than 50 km. The second one, with a 1/30° grid spacing, 100 vertical levels, takes into account the dynamics of physical scales down to 16 km. Comparison clearly emphasizes in the whole North Pacific Ocean, not only a significant KE increase by a factor up to three, but also the emergence of seasonal variability when the scale range 16–50 km (called submesoscales in this study) is taken into account. But the mechanisms explaining these KE changes display strong regional contrasts. In high KE regions, such the Kuroshio Extension and the western and eastern subtropics, frontal mixed-layer instabilities appear to be the main mechanism for the emergence of submesoscales in winter. Subsequent inverse kinetic energy cascade leads to the KE seasonality of larger scales. In other regions, in particular in subarctic regions, results suggest that the KE seasonality is principally produced by larger-scale instabilities with typical scales of 100 km and not so much by smaller-scale mixed-layer instabilities. Using arguments from geostrophic turbulence, the submesoscale impact in these regions is assumed to strengthen mesoscale eddies that become more coherent and not quickly dissipated, leading to a KE increase.     

Vertical vibration isolator having piecewise‐constant restoring force

We present a vertical vibration isolator having a piecewise‐constant restoring force, which belongs to a class of passive and nonlinear vibration isolators. In vertical vibration isolation, direct use of low‐stiffness elements leads to unacceptably large deformations due to self‐weight. To overcome the difficulty, we apply a combination of constant‐force springs, each of which sustains a constant load regardless of its stretch. By arranging the constant‐force springs, so that the isolator has a piecewise‐constant restoring force, we alleviate the problem of the excessive deformation caused by self‐weight, provide stability at the static equilibrium state along with the self‐centering capability, and realize a large stroke while keeping the mechanism simple and compact. Further, we attempt to limit the response acceleration within a tolerance regardless of the frequency spectrum and the magnitude of earthquake ground motions. We demonstrate the effectiveness of the present isolator through shaking table tests and numerical simulations. Copyright © 2009 John Wiley & Sons, Ltd.     

Nature of accretion related to Paleo-Tethys subduction recorded in northern Thailand: Constraints from mélange kinematics and illite crystallinity

We reconstructed the accretion process related to Paleo-Tethys subduction recorded in northern Thailand, based on mélange and thrust structures, and metamorphic temperatures derived from illite crystallinity data. Mélange formation was characterized by hydrofracturing and cataclastic deformation, with mud injection under semi-lithified conditions followed by shear deformation and pressure solution. Illite crystallinity data suggest metamorphic temperatures below 250 °C during mélange formation. The combined structural and metamorphic data indicate that during mélange formation, the accretionary complex related to Paleo-Tethys subduction developed at shallow levels within an accretionary prism. Asymmetric shear fabrics in mélange indicate top-to-south shear. After correction for rotation associated with collision between the Indian and Eurasian continents, the trend of the Paleo-Tethys subduction zone is estimated to have been N80 °E. We conclude that the Paleo-Tethys was subducted northward beneath the Indochina Block from the Permian to Triassic.     

Carbon isotope chemostratigraphy of a Precambrian/Cambrian boundary section in the Three Gorge area, South China: Prominent global-scale isotope excursions just before the Cambrian Explosion

Carbon isotope chemostratigraphy has been used for worldwide correlation of Precambrian/Cambrian (Pc/C) boundary sections, and has elucidated significant change of the carbon cycle during the rapid diversification of skeletal metazoa (i.e. the Cambrian Explosion). Nevertheless, the standard δ¹³C curve of the Early Cambrian has been poorly established mainly due to the lack of a continuous stratigraphic record. Here we report high-resolution δ¹³C chemostratigraphy of a drill core sample across the Pc/C boundary in the Three Gorge area, South China. This section extends from an uppermost Ediacaran dolostone (Dengying Fm.), through a lowermost Early Cambrian muddy limestone (Yanjiahe Fm.) to a middle Early Cambrian calcareous black shale (Shuijingtuo Fm.). As a result, we have identified two positive and two negative isotope excursions within this interval. Near the Pc/C boundary, the δ¹³C_carb increases moderately from 0 to + 2‰ (positive excursion 1: P1), and then drops dramatically down to − 7‰ (negative excursion 1: N1). Subsequently, the δ¹³C_carb increases continuously up to about + 5‰ at the upper part of the Nemakit–Daldynian stage. After this positive excursion, δ¹³C_carb sharply decreases down to about − 9‰ (N2) just below the basal Tommotian unconformity. These continuous patterns of the δ¹³C shift are irrespective of lithotype, suggesting a primary origin of the record. Moreover, the obtained δ¹³C profile, except for the sharp excursion N2, is comparable to records of other sections within and outside of the Yangtze Platform. Hence, we conclude that the general feature of our δ¹³C profile best represents the global change in seawater chemistry. The minimum δ¹³C of the N1 (− 7‰) is slightly lower than carbon input from the mantle, thus implying an enhanced flux of ¹³C-depleted carbon just across the Pc/C boundary. Hence, the ocean at that time probably became anoxic, which may have affected the survival of sessile or benthic Ediacaran biota. The subsequent δ¹³C rise up to + 5‰ (P2) indicates an increase of primary productivity or an enhanced rate of organic carbon burial, which should have resulted in lowering pCO₂ and following global cooling. This scenario accounts for the cause of the global-scale sea-level fall at the base of the Tommotian stage. The subsequent, very short-term, and exceptionally low δ¹³C (− 9‰) in N2 could have been associated with the release of methane from gas hydrates due to the sea-level fall. The inferred dramatic environmental changes (i.e., ocean anoxia, increasing productivity, global cooling and subsequent sea-level fall with methane release) appear to coincide with or occur just before the Cambrian Explosion. This may indicate synchronism between the environmental changes and rapid diversification of skeletal metazoa.     

Timescale of material circulation in subduction zone: U–Pb zircon and K–Ar phengite double‐dating of the Sanbagawa metamorphic complex in the Ikeda district,central Shikoku,southwest Japan

We have estimated the timescale of material circulation in the Sanbagawa subduction zone based on U–Pb zircon and K–Ar phengite dating in the Ikeda district, central Shikoku. The Minawa and Koboke units are major constituents of the high‐P Sanbagawa metamorphic complex in Shikoku, southwest Japan. For the Minawa unit, ages of 92–81 Ma for the trench‐fill sediments, are indicated, whereas the age of ductile deformation and metamorphism of garnet and chlorite zones are 74–72 Ma and 65 Ma, respectively. Our results and occurrence of c. 150 Ma Besshi‐type deposits formed at mid‐ocean ridge suggest that the 60‐Myr‐old Izanagi Plate was subducted beneath the Eurasian Plate at c. 90 Ma, and this observation is consistent with recent plate reconstructions. For the Koboke unit, the depositional ages of the trench‐fill sediments and the dates for the termination of ductile deformation and metamorphism are estimated at c. 76–74 and 64–62 Ma, respectively. In the Ikeda district, the depositional ages generally become younger towards lower structural levels in the Sanbagawa metamorphic complex. Our results of U–Pb and K–Ar dating show that the circulation of material from the deposition of the Minawa and Koboke units at the trench through an active high‐P metamorphic domain to the final exhumation from the domain occurred continuously throughout c. 30 Myr (from c. 90 to 60 Ma).     

Contamination by organochlorine compounds in sturgeons from Caspian Sea during 2001 and 2002

Organochlorine compounds (OCs) were determined in the five species of sturgeons collected from coastal waters of Caspian Sea in Kazakhstan, Azerbaijan, Turkmenistan and Iran during 2001 and 2002 to understand their status of contamination and accumulation features. Among OCs examined, concentrations of DDTs (DDT and its metabolites) were predominant in all the sturgeon samples with concentrations ranging from 73 to 31,000 ng/g on lipid weight basis, followed by PCBs, CHLs, HCHs, HCB, dieldrin, TCPMOH, and heptachlor epoxide in order. The concentrations of OCs in beluga (Huso huso) were the highest among all the five species. When comparing residue levels among same species, OC residues were highest in sturgeons from Azerbaijan and Kazakhstan and lowest in Turkmenistan. However, the concentrations of HCHs, dieldrin, and heptachlor epoxide in sturgeons showed less geographical variability.