Screening of extraction methods for glycoproteins from jellyfish (Rhopilema esculentum) oral-arms by high performance liquid chromatography

In order to select an optimum extraction method for the target glycoprotein (TGP) from jellyfish (Rhopilema esculentum) oral-arms, a high performance liquid chromatography (HPLC)-assay for the determination of the TGP was developed. Purified target glycoprotein was taken as a standard glycoprotein. The results showed that the calibration curves for peak area plotted against concentration for TGP were linear (r = 0.9984, y = 4.5895x+47.601) over concentrations ranging from 50 to 400 mgL-1. The mean extraction recovery was 97.84% (CV2.60%). The fractions containing TGP were isolated from jellyfish (R. esculentum) oral-arms by four extraction methods: 1) water extraction (WE), 2) phosphate buffer solution (PBS) extraction (PE), 3) ultrasound-assisted water extraction (UA-WE), 4) ultrasound-assisted PBS extraction (UA-PE). The lyophilized extract was dissolved in Milli-Q water and analyzed directly on a short TSK-GEL G4000PWXL (7.8 mm×300 mm) column. Our results indicated that the UA-PE method was the optimum extraction method selected by HPLC.     

七带石斑鱼胚体和卵黄囊期仔鱼的发育

本实验对七带石斑鱼胚体、卵黄囊期仔鱼形态发育的过程进行观察,研究其发育特征.结果表明:(1)七带石斑鱼受精卵在水温会(27.77±1.05)℃,盐度32条件下,胚胎发育从胚体形成至孵出仔鱼历时约22h 10min.(2)仔鱼卵黄囊在孵化2d后消失,油球消失于孵化后第6 d,卵黄囊依照先卵黄、后油球的顺序被吸收.吸收速度为先快后慢.(3)初孵仔鱼在孵化后一天时间内生长迅速.之后速度减缓.(4)仔鱼的营养可概括为内源性营养一混合营养一外源性营养阶段.     

Extending self-organizing maps for supervised classification of remotely sensed data

An extended self-organizing map for supervised classification is proposed in this paper. Unlike other traditional SOMs, the model has an input layer, a Kohonen layer, and an output layer. The number of neurons in the input layer depends on the dimensionality of input patterns. The number of neurons in the output layer equals the number of the desired classes. The number of neurons in the Kohonen layer may be a few to several thousands, which depends on the complexity of classification problems and the classification precision. Each training sample is expressed by a pair of vectors ： an input vector and a class codebook vector. When a training sample is input into the model, Kohonen＇s competitive learning rule is applied to selecting the winning neuron from the Kohouen layer and the weight coefficients connecting all the neurons in the input layer with both the winning neuron and its neighbors in the Kohonen layer are modified to be closer to the input vector, and those connecting all the neurons around the winning neuron within a certain diameter in the Kohonen layer with all the neurons in the output layer are adjusted to be closer to the class codebook vector. If the number of training sam- ples is sufficiently large and the learning epochs iterate enough times, the model will be able to serve as a supervised classifier. The model has been tentatively applied to the supervised classification of multispectral remotely sensed data. The author compared the performances of the extended SOM and BPN in remotely sensed data classification. The investigation manifests that the extended SOM is feasible for supervised classification.     

Numerical analysis of the effect of natural microcracks on the supercritical CO2 fracturing crack network of shale rock based on bonded particle models

The problem of predicting the geometric structure of induced fractures is highly complex and significant in the fracturing stimulation of rock reservoirs. In the traditional continuous fracturing models, the mechanical properties of reservoir rock are input as macroscopic quantities. These models neglect the microcracks and discontinuous characteristics of rock, which are important factors influencing the geometric structure of the induced fractures. In this paper, we simulate supercritical CO₂ fracturing based on the bonded particle model to investigate the effect of original natural microcracks on the induced‐fracture network distribution. The microcracks are simulated explicitly as broken bonds that form and coalesce into macroscopic fractures in the supercritical CO₂ fracturing process. A calculation method for the distribution uniformity index (DUI) is proposed. The influence of the total number and DUI of initial microcracks on the mechanical properties of the rock sample is studied. The DUI of the induced fractures of supercritical CO₂ fracturing and hydraulic fracturing for different DUIs of initial microcracks are compared, holding other conditions constant. The sensitivity of the DUI of the induced fractures to that of initial natural microcracks under different horizontal stress ratios is also probed. The numerical results indicate that the distribution of induced fractures of supercritical CO₂ fracturing is more uniform than that of common hydraulic fracturing when the horizontal stress ratio is small.     

铼－锇同位素体系在基性—超基性岩中应用现状的综述

综述了该同位素体系晚侏罗世以来，尤其是现代地幔岩石的研究成表明，地幔在晚侏罗世以来在１８７Ｏｓ／１８６Ｏｓ比值方面显示出非均一性，其比值从０．９０～１．２６，但此类岩石的１８７Ｏｓ／１８６Ｏｓ比值分别与各个不同研究者所确定的地幔演化线相一致；来自较古老正常地幔或贫化地幔的岩石，在锇同位素方面体现为１８７Ｏｓ／１８６Ｏｓ初始比值接近或低于Ｒｅ－Ｏｓ同位素体系地幔演化线值。在此类岩石中，Ｒｅ－Ｏｓ同位素体系与Ｓｍ－Ｎｄ同位素体系之间存在着三种可能的关系：①正相关关系；②负相关关系；③无相关关系。据认为，第一种关系是由羽状地幔端元和富集亲石元素而且贫铼之次大陆型岩石圈地幔端元混合而成；第二种关系则是由羽状地幔端元与地壳物质混合而成；第三种关系则是锇同位素成分相同但钕同位素成分明显不同的两端元物质混合而成。在第三方面，有古老俯冲大洋壳物质参与之基性－超基性岩石具有高于地幔演化线的初始１８７Ｏｓ／１８６Ｏｓ比值。具有此种锇同位素成分特征的岩石包括大洋岛弧玄武岩（ＯＩＢ）、洋中脊玄武岩（ＭＯＲＢ）以及榴辉岩。①     

关于成矿系列的结构

成矿系列是一个自然系统,它由若干矿床成因类型组成。各矿床类型间的时空结构关系表现为各类型间的“共生性”、“过渡性”和“重叠性”;其物质结构关系表现为“互补性”.本文按系统论观点,分析了上述结构,提出长江中下游铁铜成矿系列的新模式,并指出研究成矿系列的结构对预测矿产的重要意义.     

一种基于GIS的矿产资源潜力评价的自动制图模型

在基于GIS技术的矿产资源评价工作中,矿产资源潜力评价的自动制图模型通常用来统计综合多源地学信息以便自动圈定成矿远景靶区。在本文中,笔者以人工智能研究领域中的一种不确定推理模型——确定性理论为基础,提出了一种新的矿产资源潜力评价的自动制图模型——合成有矿可信度模型。该模型可以根据研究区各种成矿有利和不利证据的空间分布图,统计生成对应于每一种证据的有矿可信度栅格图,然后,按照特定的有矿可信度合成规则,将所有的有矿可信度栅格图统计综合生成合成有矿可信度栅格图。以该图为依据,可以把研究区内合成有矿可信度相对较高的成矿远景区圈定出来。也可以生成研究区合成有矿可信度等值线图。应用该模型预测了新疆北部多拉纳萨依—阿舍勒地区的多金属成矿远景,并将预测结果与证据加权模型预测结果进行了比较,两种模型的预测结果基本相似,证明了该模型的有效性和实用性。     

The Coupled Re—Os and Pt—Os Systematics：A Theoretical Consideration

The ^190Pt-^186Os system should be a unique tracer for mantle processes be-cause both Pt and Os are concentrated in the mantle.The ^190Pt-^186Os system will also be a good supplement to the ^187Re-^187Os system for dating and tracing mantle pro-cesses because the ^190Pt-^186Os system is not so easily contaminated by crustal materials as the ^187Re-^187Os system.In turn,the application of the ^187Re-^187Os system to Pt-enriched materials uncontaminated by crustal materials will indirectly refine the half life of ^190Pt.The ^190Pt is refined.In the coupled ^187Re-^187Os and ^190Pt-^186Os sys-tematics,an ^186Os*/^190Pt-^187Os*/^187Re Concordia diagram similar to the ^206Pb*/^238U-^208Pb*/^232Th Concordia can be constructed.In such a Concordia diagram,a date will be obtained so long as the ^190Pt-^186Os system remains closed even if the ^187Re-^187Os sys -tem is contaminated by crustal materials.In addition ,for the coupled ^190Pt-^186Os and ^187Re-^187Os systematics,the mantle processes and the interactions between the mantle and the crust will be described by two ratios:^186Os/^188Os and ^187Os/^188Os .The coupled ^187Re-^187Os and ^190Pt-^186Os systematics will be a powerful tool in the investigation of the geodynamic history of the Earth because the ^187Re-^187Os system is sensitive to the interactions between the mantle and the crust,while the ^190Pt-^186Os system is a good tracer for mantle processes.     

谈谈航片扫描中亮点和暗点的设置

介绍了扫描仪在测绘(地图)生产中实际操作的重点,分析了选取影像中最亮点与最暗点的重要性,阐述了其原理及在实际工作中的应用。     

刚性桩复合地基垫层对桩土应力比影响分析

从极限平衡理论角度对刚性桩复合地基中垫层作用机理进行分析,提出桩顶垫层厚度、垫层材料内摩擦角等参数对桩土应力分担的控制公式,推导出在已知表面应力情况下考虑桩土共同作用的桩土沉降计算公式,在此基础上提出一种桩土应力比的迭代计算方法。编制程序实现对算例的计算,总结分析计算结果并与实测值进行对比验证。