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41.
Does heavy oil pollution induce bacterial diseases in Japanese flounder Paralichthys olivaceus? 总被引:1,自引:1,他引:0
Jun-Young Song Kei Nakayama Yasunori Murakami Sung-Ju Jung Myung-Joo Oh Satoru Matsuoka Hidemasa Kawakami Shin-Ichi Kitamura 《Marine pollution bulletin》2008,57(6-12):889
As basic research for the effect of heavy oil on the fish immune system, in this study, the number of leukocyte was counted in Japanese flounder Paralichthys olivaceus, after exposure to heavy oil at a concentration of 30 g/8 L for 3 days. To compare the numbers of bacteria in the skin mucus between oil-exposed and control fish, viable bacteria were enumerated by counting colony forming unit (CFU). Compared with 5.79 ± 1.88 × 107 leukocytes/mL in the controls, the exposed fish demonstrated higher counts, averaging 1.45 ± 0.45 × 108 cells/mL. The bacterial numbers of control fish were 4.27 ± 3.68 × 104 CFU/g, whereas they were 4.58 ± 1.63 × 105 CFU/g in the exposed fish. The results suggest that immune suppression of the fish occurred due to heavy oil stressor, and bacteria could invade in the mucus, resulting in the increasing leukocyte number to prevent infectious disease. 相似文献
42.
Toxicogenomic analysis of immune system-related genes in Japanese flounder (Paralichthys olivaceus) exposed to heavy oil 总被引:1,自引:1,他引:0
Kei Nakayama Shin-Ichi Kitamura Yasunori Murakami Jun-Young Song Sung-Ju Jung Myung-Joo Oh Hisato Iwata Shinsuke Tanabe 《Marine pollution bulletin》2008,57(6-12):445
Heavy oil contamination is one of the most important environmental issues. Toxicities of polycyclic aromatic hydrocarbons (PAHs), including immune toxicities, are well characterized, however, the immune toxic effects of heavy oil, as a complex mixture of PAHs, have not been investigated. In the present study, we selected Japanese flounder (Paralichthys olivaceus) as a model organism, and observed alteration of immune function by the exposure to heavy oil. To analyze the expression profiles of immune system-related genes, we selected 309 cDNAs from our flounder EST library, and spotted them on a glass slide. Using this cDNA array, alteration of gene expression profiles was analyzed in the kidneys of flounders exposed to heavy oil. Six Japanese flounders (mean body weight: 197 g) were acclimated to laboratory conditions at 19–20 °C. Three fish were exposed to heavy oil C (bunker C) at a concentration of 3.8 g/L for 3 days, and the others were kept in seawater without heavy oil and used as the control. After the exposure period, the fish were transferred into control seawater and maintained for 4 days, and then they were dissected and their kidneys were removed. Total RNA was extracted from the kidney samples to use in gene expression analyses. The microarray detected alteration of immune system-related genes in the kidneys of heavy oil-exposed flounders, including down-regulation of immunoglobulin light chain, CD45, major histocompatibility complex class II antigens and macrophage colony-stimulating factor precursor, and up-regulation of interleukin-8 and lysozyme. These results suggest that pathogen resistance may be weakened in heavy oil-exposed fish, causing a subsequent bacterial infection, and then proinflammatory genes may be induced as a defensive response against the infection. Additionally, we found candidate genes for use as biomarkers of heavy oil exposure, such as N-myc downstream regulated gene 1 and heat shock cognate 71 kDa proteins. 相似文献
43.
Yuki Sugahara Masahumi Kawaguchi Tatsuya Itoyama Daisuke Kurokawa Yasuhiko Tosa Shin-Ichi Kitamura Itsuki C. Handoh Kei Nakayama Yasunori Murakami 《Marine pollution bulletin》2014
Spills of heavy oil (HO) have an adverse effect on marine life. We have demonstrated previously that exposure to HO by fertilized eggs of the pufferfish (Takifugu rubripes) induces neural disruption and behavioral abnormality in early-hatched larvae. Here, two kinds of polycyclic aromatic hydrocarbons, pyrene and phenanthrene, were selected to examine their toxic effects on larval behavior of another pufferfish species (T. niphobles). Larvae exposed to pyrene or phenanthrene exhibited no abnormalities in morphology. However, those exposed to pyrene but not phenanthrene swam in an uncoordinated manner, although their swimming distance and speed were normal. The optic tectum, a part of the midbrain, of pyrene-exposed larvae did not grow to full size. Thus, these findings are indicated that pyrene might be a contributor to the behavioral and neuro-developmental toxicity, although there is no indication that it is the only compound participating in the toxicity of the heavy oil mixture. 相似文献
44.
This paper attempts to show analytically that the energy-input spectra of damped SDOF systems and undamped MDOF systems excited by an earthquake motion can be predicted by smoothing the Fourier amplitude spectrum of the base acceleration. The spectral window for smoothing in the frequency domain for a damped SDOF system is identical with the probability density function of the time-variant or instantaneous vibration frequency resulting from non-linear hysteresis. The spectral window for an undamped MDOF system is identical with the set of squared participation factors associated with vibration modes. It was found that the increase in damping factor and the increase in participation of higher modes provide wider spectral windows, resulting in more flattened or unaltered energy-input spectra due to enhanced smoothing effects. 相似文献
45.
Mutsuo Inoue Yusuke Nakano Yoko Kiyomoto Hisaki Kofuji Yasunori Hamajima Masayoshi Yamamoto 《Journal of Oceanography》2010,66(3):425-428
A total of 21 surface water samples were collected on the east side of the East China Sea (ECS) (3 sites) and at the Tsushima
Strait (1 site), and 226Ra and 228Ra activities were measured using low-background γ-spectrometry. The 228Ra/226Ra ratios among the samples exhibited notable seasonal variation (228Ra/226Ra = 0.2–2.6) accompanying changes of salinity (31.7–34.7). Seasonal water circulation within the ECS is hypothesized to cause
the change by altering the mixing ratio of 228Ra-rich continental shelf water and 228Ra-poor Kuroshio water. 相似文献
46.
Fisheries co-management in the Shiretoko World Natural Heritage area was expanded to ecosystem-based management, in which the fisheries sector plays an essential role in management. A marine management plan was drawn up to define the management objectives, strategies to maintain major species, and methods for ecosystem monitoring. A network of coordinating organizations from a wide range of sectors was established to integrate policy measures. Experience from this case could inform ecosystem-based management in other countries where large numbers of small-scale fishers take a wide range of species under a fisheries co-management regime. 相似文献
47.
Yasunori Nejoh 《Astrophysics and Space Science》1996,235(2):245-253
The stationary ion-acoustic double layer is investigated in a plasma with an electron beam. The condition of the existence sensitively depends on the parameters such as the electron beam temperature, the ion temperature, the beam density and the effect of the trapped electrons. The properties of the double layer are also depicted. It turns out that the electron beam velocity is relatively small. This investigation predicts new findings of the ion-acoustic double layers in a plasma with an electron beam. 相似文献
48.
Kazuo Kawatate Tsunehiro Miita Yasunori Ouchi Shinjiro Mizuno 《Progress in Oceanography》1988,21(3-4)
On the basis of field records, we describe failures of current meter moorings deployed in the Tsushima Strait from 1983 to 1987. In order to avoid future failures of current meter moorings, we should communicate frequently with the fishermen's union and deploy the moorings with reserve flotation and acoustic release. 相似文献
49.
Yasunori Mahara Takuma Hasegawa Kimio Miyakawa Tomoko Ohta 《Applied Geochemistry》2008,23(12):3305-3320
Tunnel excavation at Äspö Island, Sweden, has caused severe groundwater disturbance, gradually extending deeper into the tunnel as present-day Baltic seawater intrudes through fractures connecting to the surface. However, the paleo-hydrogeochemical conditions have remained in the deep highly saline waters that have avoided mixing. A correlation has been observed between dissolved 4He concentration and Cl− ion concentration, measured every two years from 1995 to 2001 at Äspö. Groundwater mixing conditions can be examined by the correlations between 1/Cl, 36Cl/Cl, and 3H concentrations. Subsurface production is responsible for the majority of the 36Cl and excess dissolved 4He of interstitial groundwater in fractures. The secular equilibrium ratio of 36Cl/Cl in rock was theoretically estimated to be (5.05 ± 0.82) × 10−14 based on the neutron flux intensity, a value comparable to the measured 36Cl/Cl ratio in rock and groundwater. The degassing crustal 4He flux was estimated to be 2.9 × 10−8 1.3 × 10−6 (ccSTP/cm2a) using the HTO diffusion coefficient for the Äspö diorite. The 4He accumulation rate ranges from 6.8 × 10−10 (for the in situ accumulation rate) to 7.0 × 10−9 (ccSTP/(gwater · a) considering both 4He in situ production and the degassing flux, assuming 4He is accumulated constantly in groundwater. By comparing the subsurface 36Cl increase with 4He concentrations in groundwater, the 4He accumulation rate was determined from data for groundwater arriving at the secular equilibrium of 36Cl/Cl. The 4He accumulation rate was found to be (1.83 ± 0.72) × 10−8 ccSTP/(gwater · a) without determining the magnitude of degassing 4He flux. 相似文献
50.