塞曼石墨炉原子吸收法直接测定海水中的铜

本文选择酒石酸消除海水基体对铜信号的干扰,用塞曼石墨炉原子吸收法直接测定海水中的铜。从Cu的原子化曲线可知,加入酒石酸后,背景值减小,Cu原子吸收信号增强,且出现时间提前,表明酒石酸是有效的海水基体改进剂。用P.E.Z3030光谱仪测得双蒸水及海水中的元素Cu的特征质量(m。)分别为12.5pg及12.7pg;检测限分别为0.18μg/L和0.19μg/L,表明基体效应已消除,符合海洋监测要求。可用纯Cu标准溶液作校正曲线,以国家海洋局的两个标准海水样品中的Cu评定本法的准确度,相对偏差为0及-4.6%,与经典的经富集后的FAAS法的结果相比,相对偏差在3.0—6.5％范围内。用不同类型的光谱仪测定了30个海水样品,回收率在89—104％范围内。本法操作简便、快速。     

中国对虾新病害—肠结节病的观察

1991年7～8月浙江省宁波、舟山地区一些中国对虾Penaeruschinensis的养虾场中爆发了一种肠结节新病害,危害十分严重。本文报导该病发生与危害的情况以及症状、病原、组织病理观察的结果。     

东太平洋柱状沉积物的古气候和古环境记录

太平洋深海盆地的远洋沉积物在物质组成和来源上远较大陆边缘简单.由于远离大陆,又有海沟与周边大陆分隔,太平洋深海沉积物中通常不包含由河流水系搬运而来的悬浮物,因此从深海沉积物中提取古气候、古环境信息可以避免诸多地质因素相互叠加和干扰[1].深海远洋沉积物中的主要组分是风成陆源碎屑(包括火山碎屑)和来自上层海水的生源组分(降落到洋底的生物壳体)以及由海解作用形成的自生矿物[2],其中陆源碎屑的相对含量、粒度及矿物成分可以反映大气环流的强度及物源区的气候环境[1],生源组分的组成、相对含量和丰度以及种属含量变化则与表层海水的生产力和溶解作用有关.     

细菌、病毒与浮游植物相互关系及其对海洋地球化学循环的作用

本文综述了近年来国内外在海洋地球化学循环，特别是微生物环研究方面的发展现状，探讨了浮游植物，细菌，病毒相互之间的关系及其对海洋地球化学循环的影响。     

中国对虾丝状细菌的扫描电镜研究

本文应用扫描电镜研究了中国对虾丝状细菌的结构,附着方式及其对对虾的危害和与环境的关系。     

High Precision Geoid Models HKGEOID-2000 for Hong Kong and SZGEOID-2000 for Shenzhen, China

High precision geoid models HKGEOID-2000 for Hong Kong and SZGEOID-2000 for Shenzhen, China, have been developed with a hybrid approach of so-called sequential processing, using high precision GPS/leveling data, land and sea gravity anomalies, and digital terrain models. These two local geoid models have the same 1-km resolution. The estimated accuracy (external accuracy) is better than 1.7 cm for HKGEOID-2000 and 1.4 cm for SZGEOID-2000. Some common areas are covered by HKGEOID-2000 and SZGEOID-2000. So these two geoid models, along with high quality GPS/leveling data collected on the overlapping areas, can be used to detect the systematic bias between HKGEOID-2000 and SZGEOID-2000, as well as the difference between Hong Kong Principal Datum and 1956 yellow sea height datum of China, yielding RMS errors of 1.011 m and 1,003 m, respectively. Moreover, HKGEOID-2000, along with GPS ellipsoidal heights, is employed to determine the errors of the “orthometric heights” from purely trigonometric heighting, yielding an RMS error of 0.102 m. The combination of SZGEOID-2000 and GPS ellipsoidal heights has been used to replace the traditional spirit leveling and mapping, called GPS mapping.     

卫星遥感在湿地研究中的应用

近20年来, 由于人类活动加剧造成湿地严重丧失,湿地资源面临巨大压力, 遥感技术在湿地研究、动态监测等方面扮演着一个重要的角色.在对比国内外相关研究基础上从湿地监测的遥感影像数据源,遥感信息的信息识别的过程和方法,遥感影像在湿地研究中的应用3个方面对湿地遥感应用研究进行了回顾和评述.     

Rapid detection of virulent protease secreted by Vibrio anguillarum by dot enzyme-linked immunosorbent assay

Dot enzyme-linked immunosorbent assay （dot-ELISA）, indirect ELISA and Westem blot were performed to detect the virulent protease secreted by Vibrio anguillarum which was isolated from the diseased left-eyed flounder, Paralichthys olivaceous. Sensitivity results showed that dot-ELISA is a more sensitive, rapid and simple technique for the protease detection. The minimal detectable amount of protease is about 7 pg in the dot-ELISA test, while 7.8 ng in the indirect ELISA and 6.25 ng in the Westem blot respectively. Protease could be detected 2 h after incubation of V. anguillarum in the 2216E liquid medium but enzyme activity was very low at that period. From 6 to 12 h, the amount and enzyme activity of protease increased markedly and reached maximum at stationary phase. Analysis of serum samples periodically collected from the infected flounders showed that after 2 h of infection by V. anguillarum, the pathogenic bacteria could be detected in the blood of the infected flounders but no protease was found. It was 5-6 h after infection that the protease was detected in blood and then the amount increased as infection advanced. Quantitative detection of protease either incubation in the medium or from the blood of infected flounders could be accomplished in virtue of positive controls of quantificational protease standards （＂marker＂） so that the alterations ofprotease secretion both in vitro and in vivo could be understood generally. In addition, the indirect ELISA and dot-ELISA were also performed to detect V. anguillarum cells. Results indicated that the sensitivity of indirect ELISA to bacteria cells is higher than that of the dot-ELISA, and that the minimal detectable amount is approximately 10^4 cell/mL in the indirect ELISA, while 10^5 cell/mL in the dot-ELISA.     

南海中部海区次表层NO2^——N的最大值

本文根据1983年9月至1985年1月南海中部海区综合调查所获得的NO_2~--N及有关参数的观测资料,分析了该海区NO_2~--N的分布变化特征及其与环境因子的关系。结果表明,调查海区NO_2~--N含量的变化范围在0～0.54μmol/L之间,其中小于0.05μmol/L的测定值约占测定总数的82.1%,而大于0.05μmol/L测定值基本上出现在50～150m层。文中还对该海区次表层NO_2~--N最大值形成的机理作了初步探讨,指出密度跃层的终年存在、铵的氧化和浮游植物的代谢过程是调查海区次表层NO_2~--N最大值形成的主要因素。     

全新世长江水下三角洲朵体及其发育特征

本文根据长江水下三角洲地区的五个钻水及３０００ｋｍ浅地震剖面资料，运用生物地层学和地震地层学方法分析研究，获得了水下三角洲分期和三角洲朵体的分布及发育等地质信息，首次提出，长江水下三角洲沉积体由六期朵体参错迭置而成，详细阐述了水下三角洲复合体的结构特征；并结合古海岩线，孢粉气候地层，测年资料等，探讨了其发育过程及形成时代，为预测长江下水三角洲的发民菜，提供了新的基础地质依据。