牙鲆淋巴囊肿病毒靶器官上病毒结合蛋白的鉴定

利用抗牙鲆淋巴囊肿病毒单克隆抗体的间接免疫荧光检测技术,在患淋巴囊肿病牙鲆的表皮、鳃、胃、肠及肾中发现淋巴囊肿病毒的感染,以此确定为病毒感染的主要靶器官。用NP-40细胞裂解液分别抽提靶器官的总蛋白,利用病毒铺覆蛋白印迹技术(VOPBA)对靶器官上淋巴囊肿病毒结合蛋白进行鉴定,结果显示在胃、肠及肾的组织蛋白中均有1个分子量为27.8 kDa的蛋白与淋巴囊肿病毒特异结合,在鳃和表皮的组织蛋白中分别存在分子量为37.6 kDa和56.0 kDa的蛋白与淋巴囊肿病毒特异结合。推测在淋巴囊肿病毒靶器官上的这3种病毒结合蛋白有可能是病毒的受体蛋白。     

山东近海溶解态无机营养盐的分布特征研究

根据2006年8月、12月和2007年4月、10月山东近海海区4个航次海水中溶解无机营养盐等的调查资料,分析了该海域5种溶解无机营养盐(包括PO4-P,NO3-N,NO2-N,NH4-N,SiO3-Si)的平面分布和时空变化特征.结果表明,5种营养盐在胶州湾内出现高值,平面分布整体上呈现沿岸浓度高、外海浓度低的趋势;根据绝对限制和相对限制法则,春季和夏季P为限制因子,秋冬季营养盐结构发生变化,由缺P向缺Si转变;SiO3-Si浓度随季节变化逐渐增加,其他营养盐浓度在春季和夏季较低,秋季达到最大值.秋冬季溶解无机营养盐周日变化受潮汐影响明显.     

基于AM/FM/GIS的燃气管线信息系统的设计与实现

针对燃气管线建设和维护的实际需求,综合应用AM／FM／GIS技术建立燃气管线系统,以行政区划和地形等基础数据为背景,实现了燃气管线信息的可视化管理,并对系统的架构、功能以及适用性进行了讨论,为管线的建设和维护提供了高效的技术支持手段。应用结果表明AM／FM／GIS技术对燃气行业信息化建设具有重要的促进作用。     

基于GPS、GIS的道路养路费查询系统设计与开发

针对目前车辆养路费缴费方式存在的问题,提出建立基于GPS、GIS集成的道路养路费查询系统。构建基于数据服务、功能应用、系统支撑平台的三层结构体系,论述系统的主要功能,分析空间数据库、属性数据库建设中的关键技术,并给出相应的解决方案。     

基于遥感技术的大安市土地利用现状分析

随着人类活动对环境的影响,人口、资源与环境问题日益突出,由此引起的土地利用变化尤其引起普遍关注。随着遥感平台的多样化和图像分辨率的提高,遥感技术已成为土地利用现状调查研究中不可或缺的手段。遥感技术以其宏观性、实时性、周期性及综合性等特点,为快速、客观、准确地获取土地利用现状信息提供了可能。通过采用2007年大安市SPOT 5遥感影像,对大安市的土地利用类型进行解译,最终得到各类土地的分类数据,以此为基础开展土地利用现状分析。     

村镇空间结构特征分析

以平原村镇香河县、山地村镇密云县和水乡村镇昆山市为研究区, 分析了村镇空间结构的特征。建立基于网格的村镇选址及空间范围模型, 采用GIS空间分析和建模技术实现; 此外, 空间范围模型根据泰森多边形模型的思想, 提出用土地效益的概念代替距离来确定村镇的空间范围。由于考虑因素更全面, 更具体, 这两个模型可以更好地为村镇的选址与空间范围的确定提供参考。此研究为村镇规划工作提供帮助。     

基于数字高程模型不规则三角网的土方计算方法的实现

本文介绍一种基于数字高程模型(DEM)不规则三角网的土方量计算方法,其利用地形碎部点快速、方便的构建三维实体模型,计算土方精度高.     

测绘工程专业人力资源现状与毕业生需求研究

随着国民经济的快速发展,测绘行业得到了迅速发展壮大.江苏省是测绘人力资源大省,同时是测绘教育大省.通过抽样调查方法对江苏省测绘工程专业人力资源现状与毕业生需求情况进行了详细研究.研究得出江苏省测绘行业人才队伍不断壮大,未来需求量不断增加,有利于毕业生就业.     

Spatial variations of dimethylsulfide and dimethylsulfoniopropionate in the surface microlayer and in the subsurface waters of the South China Sea during springtime

Spatial variations in dimethylsulfide (DMS) and dimethylsulfoniopropionate (DMSP) were surveyed in the surface microlayer and in the subsurface waters of the low productivity South China Sea in May 2005. Overall, average subsurface water concentrations of DMS and DMSP of dissolved (DMSPd) and particulate (DMSPp) fractions were 1.74 (1.00-2.50), 3.92 (2.21-6.54) and 6.06 (3.40-8.68) nM, respectively. No enrichment in DMS and DMSPp was observed in the microlayer. In contrast, the microlayer showed a DMSPd enrichment, with an average enrichment factor (EF, defined as the ratio of the microlayer concentration to subsurface water concentration) of 1.40. In the study area, none of the sulfur components were correlated with chlorophyll a. An important finding in this study was that DMS, DMSP and chlorophyll a concentrations in the surface microlayer were respectively correlated with those in the subsurface water, suggesting a close linkage between these two water bodies. The ratios of DMS:Chl-a and DMSPp:Chl-a showed a gradually increasing trend from North to South. This might be due to changes in the proportion of DMSP producers in the phytoplankton community with the increased surface seawater temperature. A clear diurnal variation in the DMS and DMSP concentrations was observed at an anchor station with the highest concentrations appearing during the day and the lowest concentrations during the night. The higher DMS and DMSP concentrations during daytime might be attributed to the light-induced increase in both algal synthesis and exudation of DMSP and biological production of DMS. The mean flux of DMS from the investigated area to the atmosphere was estimated to be 2.06 micromo lm(-2)d(-1). This low DMS emission flux, together with the low DMS surface concentrations was attributed to the low productivity in this sea.     

In vivo neutralization assays by monoclonal antibodies against white spot syndrome virus in crayfish(Cambarus proclarkii)

The neutralizing activities of eight monoclonal antibodies （MAbs） against white spot syndrome virus （WSSV） （2D2, 2B2,1D2, 1D5, 1C2, 4A1, 6A4 and 6B4） were analyzed by in vivo experiments. Gills from WSSV-infected shrimp were homogenized and ten-fold serially diluted by PBS, and then incubated with MAbs （hybridoma culture supernatant）, respectively. The mixture of WSSV and MAbs were injected into crayfish （Procambarus clarkii）. After challenge, the death rates of crayfish were counted to determine the neutralizing activities of MAbs. At the same time, the mixture of myeloma culture supernatant and WSSV or PBS was served as positive or negative control, respectively. The results showed that, at each virus dilution, the mean time to death of the crayfish injected with MAb-treated virus was significantly longer than that in the positive control, though they all showed 100% mortality within 25 d, and meanwhile, few crayfish died in the negative control. Among the eight MAbs, 2D2, 2B2, 1D2 and 1D5, especially the former two, delayed the mortality significantly, and 1 C2, 4A1 and 6A4 delayed the mortality as well but not so efficiently, while MAb 6IM was efficient only when the virus concentration increased. The results indicated that the anti-WSSV MAbs can neutralize WSSV in different virus dilutions.