南海沉积物细菌胞外蛋白酶在家族水平上的多样性

海洋产蛋白酶细菌及其分泌的胞外蛋白酶在降解有机氮以推动海洋氮循环进行方面发挥着重要作用,但目前对这二者多样性的认识非常有限。本研究自南海沉积物中筛选得到90株产蛋白酶细菌,并通过N-端氨基酸序列,分析了其胞外蛋白酶在家族水平上的多样性。16S rRNA基因序列分析的结果表明,筛选的产蛋白酶细菌均属于Gammaproteobacteria纲,且大多数属于Alteromonadales目和Vibrionales目的不同属。对其中14株菌株的14个胞外蛋白酶的N-端氨基酸序列分析表明,所有这些蛋白酶属于金属蛋白酶的M4家族或丝氨酸蛋白酶的S8家族。本研究提供了海洋沉积物产蛋白酶细菌在类群及其胞外蛋白酶在类型上的新细节,这将有助于全面了解微生物酶促降解海洋沉积物有机氮的过程和机理。     

Lateral dynamic response of a partially embedded pile subjected to combined loads in saturated soil

In this article, an analytical solution is proposed to investigate the lateral dynamic response of a pile which is partially embedded in saturated soil layer and subjected to combined lateral and vertical loads. The saturated soil is described by Biot’s poroelastic theory and the resistance of soil is derived by potential function method. The governing equation of the pile is solved by coupling soil resistance and continuity conditions between the pile and the soil. The dynamic impedances of the pile are then obtained through transfer matrix method. To verify the validity of the proposed procedure, the present solution is compared with available solution for an idealized case. Finally, a parametric study is performed to investigate the effects of various parameters on the stiffness and damping properties of the pile-soil system. It is found that permeability of the soil and vertical load has significant effects on the dynamic response of the pile.     

Analytical Theory for One-Dimensional Consolidation of Soil Induced by Time-Dependent Pumping and Loading

Analytical solutions are derived for one-dimensional consolidation induced by time-dependent pumping and loading, in which both the effects of different pumping types and loading conditions are considered, i.e., step pumping, cyclic pumping, constant loading, and step loading. Based on the solutions obtained, some diagrams are prepared and the relevant consolidation behavior of soil is discussed in detail. It has been shown that the consolidation behavior is greatly influenced by pumping velocity, loading pressure, pumping and loading type.     

Geophysical investigations of crust-scale structural model of the Qiongdongnan Basin, Northern South China Sea

Rifting of the Qiongdongnan Basin was initiated in the Cenozoic above a pre-Cenozoic basement, which was overprinted by extensional tectonics and soon after the basin became part of the rifted passive continental margin of the South China Sea. We have integrated available grids of sedimentary horizons, wells, seismic reflection data, and the observed gravity field into the first crust-scale structural model of the Qiongdongnan Basin. Many characteristics of this model reflect the tectonostratigraphic history of the basin. The structure and isopach maps of the basin allow us to reconstruct the history of the basin comprising: (a) The sediments of central depression are about 10 km thicker than on the northern and southern sides; (b) The sediments in the western part of the basin are about 6 km thicker than that in the eastern part; (c) a dominant structural trend of gradually shifting depocentres from the Paleogene sequence (45–23.3 Ma) to the Neogene to Quaternary sequence (23.3 Ma–present) towards the west or southwest. The present-day configuration of the basin reveals that the Cenozoic sediments are thinner towards the east. By integrating several reflection seismic profiles, interval velocity and performing gravity modeling, we model the sub-sedimentary basement of the Qiongdongnan Basin. There are about 2–4 km thick high-velocity bodies horizontal extended for a about 40–70 km in the lower crust (v > 7.0 km/s) and most probably these are underplated to the lower stretched continental crust during the final rifting and early spreading phase. The crystalline continental crust spans from the weakly stretched domains (about 25 km thick) near the continental shelf to the extremely thinned domains (<2.8 km) in the central depression, representing the continental margin rifting process in the Qiongdongnan Basin. Our crust-scale structural model shows that the thinnest crystalline crust (<3 km) is found in the Changchang Sag located in the east of the basin, and the relatively thinner crystalline crust (<3.5 km) is in the Ledong Lingshui Sag in the west of the basin. The distribution of crustal extension factor β show that β in central depression is higher (>7.0), while that on northern and southern sides is lower (<3.0). This model can illuminate future numerical simulations, including the reconstruction of the evolutionary processes from the rifted basin to the passive margin and the evolution of the thermal field of the basin.     

Effect of Groundwater Fluctuations on Pore Pressures and Earth Pressures on Coastal Excavation Retaining Walls

Pore water and earth pressures acting on retaining structures are investigated using an efficient coastal double-layered excavation model to determine offshore excavation responses to groundwater fluctuations outside foundation pits. Total pore water pressure includes excess pore water pressure (due to groundwater fluctuations) and steady pore water pressure (due to steady seepage) determined using one-dimensional consolidation theory of double-layered soil and one-dimensional steady-state flow theory, respectively. Rankine's active and passive earth pressures are obtained from pore water pressure. This method is applicable to arbitrary groundwater fluctuation conditions. How physical parameters affect pore water pressure is numerically investigated using examples, demonstrating the method's practicality for calculating pore water and earth pressures.     

Dynamic Response Analysis and Vibration Control for A Fixed-Bottom Offshore Wind Turbine Subjected to Multiple External Excitations

For the offshore wind turbines installed in earthquake areas, their operation is affected by seismic loads in addition to wind and wave loads. Therefore, it is necessary to study the dynamic responses and vibration control of the wind turbines. In previous studies, the structural responses of offshore wind turbines are usually investigated in the parked case, while the blade rotation effect is usually not considered. The evaluation on the structural responses may be inaccurate under this conditi...     

Oceanic internal solitary waves at the Indonesian submarine wreckage site

<正>On 21 April 2021 local time(20 April UTC), the Indonesian Navy submarine(KRI Nanggala-402) sank near the Lombok Strait, ～100 km north of the Bali Island(see magenta star in Fig. 1a),with 53 crew members dead. On the basis of Moderate Resolution Imaging Spectroradiometer(MODIS) satellite images(Jackson, 2007),     

空心块体水沙动力及泥沙淤积特性研究

空心块体具有良好的阻水和促淤功能,近年来被广泛用于生态修复工程。本文结合水槽试验及Flow-3D数值模拟,分析了开敞型和半封闭型空心块体的阻水效应和泥沙淤积特性。结果表明：空心块体的开孔率对内部水流流速、紊动强度起主导作用,开孔率较小的半开敞型空心块体减速、制紊效果更强;开敞型和半封闭型空心块体近底层悬沙浓度分别增大56%和75%,两者均有利于促进泥沙在块体内部淤积,近底层水流紊动越强,泥沙淤积形态差异越大;空心块体所营造的低流速、泥沙微淤、内外连通的水沙环境是大型底栖生物的生境需求,半封闭型空心块体内部的低紊动水流结构更有利于大型底栖生物的栖息、繁衍。     

石榴石橄榄岩的熔融实验及其地质意义

在20×10~9Pa和25×10~9Pa条件下,对石榴石橄榄岩进行的14次高温高压熔融实验研究表明,在1600℃时石榴石橄榄岩开始熔融(初熔),熔体中的SiO_2>70％,Na_2O+K_2O>8％,类似于A型花岗岩成分。本实验为地幔花岗岩的成因提供了证据。