海河流域夏季降水与北半球环流异常的相关

使用1981—2013年6—8月的月平均海河流域站点降水资料以及NCEP/NCAR再分析资料,采用SVD方法分析了北半球高度场环流异常与海河流域夏季降水的关系。结果表明夏季海河流域的一致型降水异常主要受热带地区高度场异常的影响,而北半球中高纬的高度异常分布对海河流域东南部降水异常有重要作用。热带及中高纬地区的高度场异常与副热带西风急流和流域上空的水汽输送有一定的对应关系,急流强度和位置的变化以及水汽辐合异常造成海河流域降水异常,尤其中高纬高度异常的这种对应关系更加明显,并且两个地区的高度异常分布对应的海河流域异常水汽输送有不同来源。     

基于 ArcG IS 和 Python 集成开发可达性分析工具的研究

为降低可达性度量的实现难度,弥补现有GIS软件没有集成可达性分析模型或方法的不足,在详尽分析有关模型或方法与GIS集成研究现状的基础上,提出以改进潜能模型作度量模型,按GIS内部集成方式,使用面向ArcGIS 10的Python脚本编程技术集成开发可达性分析工具的设想,对可达性潜能模型分析原理、工具开发环境配置、使用数据的预处理要求、工具的具体设计流程和实现方法等作了详细叙述。最后以评价某市小学教育资源空间布局合理性为例,阐述工具在实际应用中应采取的使用方法和流程,实例评价结果验证工具开发的有效性。     

Distinct groundwater recharge sources and geochemical evolution of two adjacent sub-basins in the lower Shule River Basin,northwest China

Based on analysis of groundwater hydrogeochemical and isotopic data, this study aims to identify the recharge sources and understand geochemical evolution of groundwater along the downstream section of the Shule River, northwest China, including two sub-basins. Groundwater samples from the Tashi sub-basin show markedly depleted stable isotopes compared to those in the Guazhou sub-basin. This difference suggests that groundwater in the Tashi sub-basin mainly originates from meltwater in the Qilian Mountains, while the groundwater in the Guazhou sub-basin may be recharged by seepage of the Shule River water. During the groundwater flow process in the Tashi sub-basin, minerals within the aquifer material (e.g., halite, calcite, dolomite, gypsum) dissolve in groundwater. Mineral dissolution leads to strongly linear relationships between Na⁺ and Cl^? and between Mg²⁺+ Ca²⁺ and SO₄ ^2??+?HCO₃ ^?, with stoichiometry ratios of approximately 1:1 in both cases. The ion-exchange reaction plays a dominant role in hydrogeochemical evolution of groundwater in the Guazhou sub-basin and causes a good linear relationship between (Mg²⁺+ Ca²⁺)–(SO₄ ^2??+?HCO₃ ^?) and (Na⁺+ K⁺)–Cl^? with a slope of ?0.89 and also results in positive chloroalkaline indices CAI 1 and CAI 2. The scientific results have implications for groundwater management in the downstream section of Shule River. As an important irrigation district in Hexi Corridor, groundwater in the Guazhou sub-basin should be used sustainably and rationally because its recharge source is not as abundant as expected. It is recommended that the surface water should be used efficiently and routinely, while groundwater exploitation should be limited as much as possible.     

Effect of Groundwater Fluctuations on Pore Pressures and Earth Pressures on Coastal Excavation Retaining Walls

Pore water and earth pressures acting on retaining structures are investigated using an efficient coastal double-layered excavation model to determine offshore excavation responses to groundwater fluctuations outside foundation pits. Total pore water pressure includes excess pore water pressure (due to groundwater fluctuations) and steady pore water pressure (due to steady seepage) determined using one-dimensional consolidation theory of double-layered soil and one-dimensional steady-state flow theory, respectively. Rankine's active and passive earth pressures are obtained from pore water pressure. This method is applicable to arbitrary groundwater fluctuation conditions. How physical parameters affect pore water pressure is numerically investigated using examples, demonstrating the method's practicality for calculating pore water and earth pressures.     

深海细菌太平洋火色杆菌中α-淀粉酶基因amy608的克隆表达及其酶学性质研究

从来自深海沉积物的太平洋火色杆菌(Flammeovirga pacifica)的基因组中发现了一个全长为1 875 bp的α-淀粉酶基因amy608.该基因在数据库中找不到具有同源性的序列,而所编码的Amy608蛋白与已注册蛋白的氨基酸序列相似性最高仅为56%,但具有α-淀粉酶水解活性所必需的保守基序DXEXD.进化树分析表明其属于糖苷水解酶13(GH13)家族第二亚家族.构建了p ColdΙ-amy608表达载体,在大肠杆菌中进行重组Amy608蛋白的异源表达,并采用镍离子亲和层析柱对重组蛋白进行了纯化.酶学性质分析表明,重组酶Amy608的最适作用温度为40℃,在40℃保温4 d后,仍保留70%以上的酶活,显示出良好的中温热稳定性;最适p H值为7.0,p H值范围在6~9时仍保留60%以上的酶活力,表明该酶具有较宽的p H值作用范围.Ca2+、Na+、K+对α-淀粉酶Amy608有激活作用,尤其是Ca2+可使酶活显著提高40%.薄层色谱分析结果显示该酶水解可溶性淀粉的最终产物以葡萄糖为主,表明该酶是一个糖化型淀粉水解内切酶.这些结果表明Amy608为GH13家族第二亚家族中一个新型的α-淀粉酶.