基于羟基自由基高级氧化快速杀灭锥状斯氏藻孢囊的研究

随着船舶压载水的转运,我国面临严重的外来入侵生物风险,其中部分赤潮藻形成的孢囊可成为赤潮的"种源",严重危害近岸海洋环境,因而快速有效地杀灭赤潮藻孢囊至关重要。本文利用大气压强电离放电高效生成的羟基自由基（·OH）,对典型赤潮藻锥状斯氏藻孢囊进行杀灭研究。采用萌发实验、SYTOX Green荧光染色法确定·OH杀灭锥状斯氏藻孢囊的阈值和时间,扫描电子显微镜观察孢囊的形态变化。结果表明,·OH杀灭孢囊的CT阈值为0.49 mg·min/L,时间为10 s,·OH氧化降解孢囊体内叶绿素,破坏DNA,抑制萌发,具有其他方法无法比拟的优势。因此,·OH快速杀灭赤潮藻孢囊的新方法,对防控外来入侵生物引发的海洋赤潮灾害具有重要的作用。     

龙永煤田危机矿山资源接替的几种勘探模式

通过龙永煤田现有煤矿状况调查分析发现：许多矿井已步入“花甲之年”，接近矿井服务年限。为了保障我省能源安全，应当采用有效勘探方法，方能使它们“返老还童”。研究发现危机矿山延长服务年限的几种勘探模式是：其一为扩大（延伸）勘探模式；其二为小型井田（块段）勘探模式；其三为资料分析研究模式。经探采对比发现，采用这三种勘探模式后，产生了巨大的经济效益，使一大批老矿井青春再度焕发。     

钻井液技术展望

介绍了水基钻井液,油基钻井液和合成基钻井液的特点及发展趋势。     

陕西柞水下梁子类卡林型金矿床特征与成因探讨

下梁子金矿床为叠加改造式层控类卡林型金矿床,集沉积地层、岩浆活动、脆韧性剪切构造活动三位一体;地层岩性含硫铁、含钙质为有利含矿岩系,为成矿准备了物质基础;岩浆作用导致围岩的夕卡岩化-角岩化,使岩石成分重组,利于成矿物质的活动;成矿以发育含矿热液的脆韧性递进剪切变形构造控制为关键,直接控制了工业矿体的分布、矿化强度与就位.研究中确定了碰撞型花岗岩与成矿的直接关系;具体划分了矿石类型,矿化期与矿化阶段,金的赋存状态等.     

锇粉中杂质元素分析方法研究

利用ＩＣＰ＿ＭＳ技术建立了锇粉中痕量杂质元素的分析方法。在浓ＨＮＯ３介质中,锇以ＯｓＯ４形式挥发除去,分离锇基体后,可直接测定的痕量元素达５０余种。取样量０．１ｇ,测定限为０．２～２４６ｎｇ／ｇ,可实现质量分数ｗ（Ｏｓ）为９９．９９９９％锇粉中杂质元素测定。结合ＩＣＰ＿ＡＥＳ测定Ｋ、Ｎａ、Ｃａ、Ｍｇ、Ａｌ、Ｆｅ、Ｓｉ和Ｐ,可实现锇粉原料及产品的纯度分析。     

缢蛏天然抗性相关巨噬蛋白2(Sc-Nramp2)基因克隆及其在副溶血弧菌刺激下的表达分析

克隆得到缢蛏天然抗性相关巨噬蛋白2（Sc-Nramp2）基因的cDNA全长序列3 681 bp,该基因的开放阅读框有1 776 bp,编码591个氨基酸,预测分子量为65.86 kDa;其结构具有Slc11蛋白家族的典型特征,包括有10个典型的跨膜结构和2个糖基化位点。Sc-Nramp2基因3'-UTR有2个类似于脊椎动物Nramp2中铁反应控制蛋白结合位点;同源性分析表明,Sc-Nramp2和太平洋牡蛎Nramp2-like的同源性最高为71.6%。实时荧光定量PCR结果表明,Sc-Nramp2基因在闭壳肌、外套膜、肝胰腺、斧足、水管和鳃6个组织中均有表达,其中肝胰腺中的表达量最高,其次是鳃,与其他组织均有极显著性差异（P<0.01）;注射副溶血弧菌后,肝胰腺中Sc-Nramp2基因的表达量较对照组显著上调（P<0.01）,且表达量呈现先上升后下降的趋势,在12 h时达到最大表达量,推测Sc-Nramp2基因参与了缢蛏非特异性免疫应答反应。     

Tectonic Units and Their Fundamental Characteristics on the Northern Margin of the Alxa Block

The northern margin of the Alxa block is the junction of a tectonic units. Four first-order tectonic units are distinguished: 1. the Yagan structural zone characteristic of an immature island arc; 2. the Zhusileng-Hangwula structural zone, which was a passive continental margin in the Early Palaeozoic and was transformed into an active continental margin in the Late Palaeozoic;3. the Shalazha structural zone characteristic of a mature island arc; 4. the Nuru-Langshan structural zone, which was a Proterozoic orogenic belt and later evolved into an extensional transtional crust in the Palaeozoic. The above-mentioned tectonic units differ remarkably in sedimentary formations, magmatic rock associations, metamorphism and geochemistry and are bounded by faults between one another.     

生物生态防治虾病研究

本文探索虾病防治新途径,采用低值海藻江蓠和对虾混养的生态学方法,江蓠吸收水中二氧化碳进行光合作用,产生大量氧气;同时吸收有机物的分解物 NH_3—N,加速有机物分解。减少 H_2S 积累;从而消除水中紧迫因子威胁,大大减少池水自污及病害,起到抑菌、抑虫、净化水质作用,提高成活率,试验圹对虾成活率达95.1%,对照圹56.6%。试验结果表明,虾病防治效果显著,经济效益明显。     

准噶尔盆地古隆起的分布与基本特征

准噶尔盆地外侧三大边界动力体系的作用及基底结构的差异性导致准噶尔盆地在不同地质时期发育不同性质的隆起构造。石炭纪晚期东北侧的造山作用形成了盆地北部隆起,晚二叠世北部隆起分异为陆梁隆起与乌伦古坳陷;早二叠世西北侧的造山作用形成了车排子隆起,随后的冲断作用形成了克拉玛依——夏子街断裂带。燕山期的压扭作用形成了沉积盖层中的车排子——莫索湾隆起。新生代北天山向北的冲断作用导致准噶尔盆地南部地区急剧挠曲沉降,盆地向南掀斜,隆起向北迁移。据基底的性质、形成时间、活动方式、地质结构与保存状态等特点将准噶尔盆地的隆起划分为继承型、间断型、掀斜型与冲断带型等4种基本类型。     

Geochemical characteristics of trace elements and tectonic setting of sandstones from the Upper Ordovician Pingliang Formation,southwestern margin of Ordos Basin: A case study of the Duanjiaxia profile,Longxian County

Due to the lack of reliable sedimentary provenance data,the geological setting and its provenance of the southwestern margin of Ordos Basin in Late Ordovician is uncertain. In this paper,the sandstone of Upper Ordovician Pingliang Formation in Duanjiaxia profile of Longxian area is selected for a systematic study. Using the methods including the comprehensive analysis on the measured geological profile,analysis of microscopic thin section and trace elements,the following results are obtained: (1)the thin section analysis shows that the sandstone is well sorted but poorly rounded. Clastic grains are dominated by quartz,while the rock fragments are mainly composed of polycrystalline quartz,carbonate sand debris,and a little matrix,which means that the turbidite sandstone has been reworked by obvious traction of deep-water;(2)Rare Earth Element(REE)distribution model shows that the sandstone is rich in LREE and is short of HREE and has the negative Eu-anomaly,indicating that the provenance may derive from the intermediate and acid rock in the upper crust;(3)the provenance discrimination diagrams show that the sandstone of the Pingliang Formation is a mixture of sedimentary rock and granite,while the magmatic component is dominated by intermediate and acid rock;(4)Geochemical data of the sandstone suggests that the geological setting significantly belong to continental island arc and passive continental settings. The study area in Longxian during the Late Ordovician period was located in the back arc basin. At that time,the southwestern margin of the North China Craton with the full trench-arc-basin system was changed from the passive continental margin into the active continental margin.