南极菲尔德斯半岛潮间带沉积物细菌的多样性及分离菌产酶测定

摘要:南极菲尔德斯半岛具有多种不同特征的生态地理微环境,如长城科考站、企鹅岛、生物湾与黄金湾流域、半岛南部、碧玉滩等。这些区域因水文地质、动植物分布、人类活动程度等不同而具有明显的生态地理差异。本研究从这些微环境海岸潮间带采集了7份代表性沉积物样品,采用16S rRNA基因高通量测序方法,对其细菌类群的多样性以及环境理化因子的影响进行了比较分析;同时采用常规可培养鉴定方法,对样品的分离菌株产酶状况进行了初步测定。结果表明：整体上看,所有样品的细菌类群主要分布在45门、104纲、442属当中,表现出比较高的多样性。其中,优势菌门Proteobacteria主要分布在半岛南部、碧玉滩和生物湾潮间带沉积物物中,优势菌门Bacteroidetes主要分布在企鹅岛两侧和黄金湾潮间带沉积物,长城站潮间带沉积物的优势门为Firmicutes。相似生态微环境的潮间带沉积物具有不同的优势菌门和纲;而不同生态微环境潮间带沉积物却具相同的优势菌门和纲。有机氮（TON）、NH4+-N、NO3--N、PO43--P、NO2--N含量与企鹅岛两侧、生物湾、黄金湾潮间带沉积物的菌群多样性具有相关性,其中TON对生物湾样品的影响最大,而有机碳（TOC）对所有样品的影响较小。分离菌株的产酶实验表明：在企鹅岛、生物湾、黄金湾等动物频繁出没的潮间带样品中,蕴藏着一批产淀粉酶、酪蛋白酶、脂肪酶、七叶苷酶、过氧化氢酶能力较强菌株,这为今后进一步的应用开发提供了宝贵的低温产酶菌株来源。 关键词: 菲尔德斯半岛; 潮间带沉积物; 细菌类群多样性; 产酶菌株     

Historical Sediment Record of ~(137)Cs, δ-HCH,and δ~(13)C Reflects the Impact of Land Use on Soil Erosion

This paper reports the concentrations of 137 Cs, hexachlorocyclohexane(HCH), dichlorodiphenyltrichloroethanes(DDT) and its main degradation products, δ13C, and organic carbon in pond sediments(0-210 cm, sectioned by 2-20 cm interval) and surface soils(the 0-3 cm horizon) collected in 2010 from Chenjia catchment, which is located in Yanting county in the hilly central Sichuan of China. α-, δ-, and γ-HCH, DDT, and DDD were not detected throughout the sediment profile. Trace concentrations of δ-HCH(0.89-29.31 ng g-1) and p,p′-DDE(1.85-6.02 ng g-1) were detected only in top 40 cm sediment. The 137 Cs fallout peak in 1963(corresponding to the 55-60 cm depth), the sedimentary signature left by the last year of HCH use in 1989(an additional indicator at 20-25 cm), and the obvious original channel bed prior to the construction of the pond in 1956 were used as temporal markers to estimate changes in average sedimentation rate between different periods due to changes in land use. Continuous, marked decrease in average sedimentation rate(i.e., 3.79, 1.35 and 1.07 cm year-1 in 1956-1963, 1963-1989, and 1989-2010, respectively)over time was observed, probably due to the reforestation, abandoning of steep sloping farmland for afforestation and natural re-vegetation(implementation of the Grain for Green Program), and the conversion of part of gently sloping farmland terraces to orchard land since the 1980 s, especially since the 1990 s. This was corroborated by the observed decrease(more negative) in δ13C of sediment towards the surface, which indicates increased relative contribution of eroded soil particles coming from slopes with increased tree cover in sediment source area. Combined use of 137 Cs, δ-HCH, and δ13C record in sediments has been demonstrated to be a powerful approach to reconstruction of response in sedimentation rate to historical land use changes.     

渤海海峡亚海鲂属鱼类一新记录种——云纹亚海鲂

2012年12月3日,在渤海海峡的蓬莱近海浅水海域捕获雄性成体鱼1尾,该鱼体长34 cm,体高18cm,体长为体高的1.9倍,体宽4.5 cm,头长12.5 cm,体长为头长的2.7倍,尾柄长4.5 cm,体光滑无鳞。管状侧线前半部弧形,后半部平直,行于体中央。棘状骨板沿背鳍基底两侧各12块,臀鳍基底两侧各8块,每一骨板中央具1棘,向后;胸部6块,自第3骨板开始分左右两行;腹部7块,自第2骨板开始分左右两行,胸腹部骨板棘小,形成锯齿缘。背鳍1个,鳍棘部与鳍条部之间有一深缺刻;鳍棘细长,棘间鳍膜延长成丝状,最长超过尾鳍末端。据其形态特征和测量值,鉴定为云纹亚海鲂(Zenopsis nebulosa Temminck et Schlegel,1847),为该海域首次记录。     

莺歌海盆地前新生代基底特征

结合盆缘露头、盆地基底钻井及重磁震资料,综合研究莺歌海盆地前新生代基底特征。基于盆地西北缘Song Da带18个剖面点地层序列、166件岩石样品密度和2800套磁化率测量结果,建立密度--磁化率交汇图版,约束盆内地震剖面和重磁异常解释,通过海陆结合的方法填制出盆地基底地质图。其前新生界由前震旦系、寒武系-上三叠统下部、上三叠统上部-白垩系三个构造层构成,它们沿红河断裂呈北西向分布,中间老,为前震旦纪中-高级变质岩;两侧新,向西为中生代沉积岩,向东为古生代浅变质岩、灰岩。     

农业技术扩散中的农户采用行为研究:国外进展与国内趋势

农户是我国农区的微观经济单元,是农业技术采用的行为主体。以农户为对象的农业技术扩散采用行为的研究对于诱导农户行为、提高农业技术的采用效率、促进农区发展具有重要的理论和应用价值。从研究视角、研究方法、研究内容3个方面对国内外相关研究成果进行较为系统的梳理,总结评述最新进展和特点,并在此基础上对农户技术采用行为的研究趋势进行展望,希冀有助于推进国内对农户技术采用行为的深入研究,进而为促进农业技术扩散与推广、提高农业科技成果转化率提供参考。     

根据漂流浮标资料对黑潮15m层流路及流轴特征的分析

利用1979-02—2012-03共33a的水帆位于15m层的Argos漂流浮标资料,绘制黑潮流系15m层的多年年平均和月平均流场,运用特征线方法计算得到黑潮流轴,定义黑潮流动路径的边界为流速大小20~30cm/s的过渡性区域。结果显示:黑潮多年年平均流路大致是一个以(13°30′N,142°00′E)为圆心、2 235km为半径的直角弧段,其在吕宋海峡、台湾东北、九州西南及伊豆海岭附近海区发生气旋式弯曲前先进行反气旋式弯曲调整,弯曲处出现的路径开口主要是支流的并入或分支的流出;黑潮流轴整体性偏向黑潮左边界,其中在吕宋岛东北至台湾以东海域最为显著,在本州岛以南海域次之,而在东海段基本居中;黑潮流路上的流速在总体上由南向北呈增大趋势,但并非沿流路持续性逐渐增加,而是呈现出较平直流段的大流速区和弯曲调整流段的低流速区相互交错的状况,其中四国岛以南至伊豆诸岛以西流段的流速为最大。多年月平均流场显示,2月,5月,8月和11月这4个月份是黑潮流路和流轴发生变化的重要转折期,而1月,4月,7月和10月这4个月份则是各季节的代表月份。其中,冬季月份的黑潮流路和流轴最为曲折,向边缘海发生显著入侵;夏季月份的黑潮流路和流轴最为平直,左侧伴随有北向流动;春、秋两季的过渡性特征则比较明显。     

Modeling of spatial distributions of farmland density and its temporal change using geographically weighted regression model

This study used spatial autoregression（SAR） model and geographically weighted regression（GWR） model to model the spatial patterns of farmland density and its temporal change in Gucheng County,Hubei Province,China in 1999 and 2009,and discussed the difference between global and local spatial autocorrelations in terms of spatial heterogeneity and non-stationarity. Results showed that strong spatial positive correlations existed in the spatial distributions of farmland density,its temporal change and the driving factors,and the coefficients of spatial autocorrelations decreased as the spatial lag distance increased. SAR models revealed the global spatial relations between dependent and independent variables,while the GWR model showed the spatially varying fitting degree and local weighting coefficients of driving factors and farmland indices（i.e.,farmland density and temporal change）. The GWR model has smooth process when constructing the farmland spatial model. The coefficients of GWR model can show the accurate influence degrees of different driving factors on the farmland at different geographical locations. The performance indices of GWR model showed that GWR model produced more accurate simulation results than other models at different times,and the improvement precision of GWR model was obvious. The global and local farmland models used in this study showed different characteristics in the spatial distributions of farmland indices at different scales,which may provide the theoretical basis for farmland protection from the influence of different driving factors.     

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氧化多壁碳纳米管对水中钠离子的吸附研究

本文研究了强酸(体积比为3︰1的硫酸与硝酸)氧化的多壁碳纳米管对水中钠离子的吸附特性,考察了温度、初始浓度、吸附时间对钠离子吸附的影响。实验结果表明:多壁碳纳米管对钠离子的吸附能力随温度升高而下降;其吸附过程符合Langmuir吸附等温模型,但是在钠离子浓度较高时多壁碳纳米管的吸附特性更符合Freundlich等温模型;在温度为274K条件下,多壁碳纳米管对钠离子的吸附能约为-5.53KJ/mol即-57.3meV;化学反应焓变约为-9.31KJ/mol,表明混酸氧化的多壁碳纳米管对钠离子的吸附过程基本为物理吸附。     

Surface rainfall and cloud budgets associated with mei-yu torrential rainfall over eastern China during June 2011

Surface rainfall and cloud budgets associated with three heavy rainfall events that occurred over eastern China during the mei-yu season in June 2011 were analyzed using 2D cumulus ensemble model simulation data.Model domain mean rainfall showed three peaks in response to three prescribed ascending motion maxima,primarily through the mean moisture convergence during the torrential rainfall period.Prescribed ascending motion throughout the troposphere produced strong convective rainfall during the first （9 June） and third （17-18 June） rainfall events,whereas strong prescribed ascending motion in the mid and upper troposphere and weak subsidence near the surface generated equally important stratiform and convective rainfall during the second rainfall event （14 June）.The analysis of surface rainfall budgets reveals that convective rainfall was associated with atmospheric drying during the first event and moisture convergence during the third event.Both stratiform and convective rainfall responded primarily to moisture convergence during the second event.An analysis of grid data shows that the first and third mean rainfall maxima had smaller horizontal scales of the precipitation system than the second.