Processes Causing the Temporal Changes in Si/N Ratios of Nutrient Consumptions and Export Flux during the Spring Diatom Bloom

Two processes are generally explained as causes of temporal changes in the stoichiometric silicon/nitrogen (Si/N) ratios of sinking particles and of nutrient consumption in the surface water during the spring diatom bloom: (1) physiological changes of diatom under the stress of photosynthesis of diatom and (2) differences of regeneration between silicon and nitrogen. We investigated which process plays an important role in these changes using a one-dimensional ecosystem model that explicitly represents diatom and the other non-silicious phytoplankton. The model was applied to station A7 (41°30′ N, 145°30′ E) in the western North Pacific, where diatom regularly blooms in spring. Model simulations show that the Si/N ratios of the flux exported by the sinking particles at 100 m depth and of nutrient consumptions in the upper 100 m surface water have their maxima at the end of the spring diatom bloom, the values and timings of which are significantly different from each other. Analyses of the model results show that the differences of regeneration between silicon and nitrogen mainly cause the temporal changes of the Si/N ratios. On the other hand, the physiological changes of diatoms under stress can hardly cause these temporal changes, because the effect of the change in the diatom's uptake ratio of silicon to nitrogen is cancelled by that in its sinking rate.     

A Zonal Pathway for NADW in the South Atlantic

Several large deployments of neutrally buoyant floats took place within the Antarctic Intermediate (AAIW), North Atlantic Deep Water (NADW), and the Antarctic Bottom Water (AABW) of the South Atlantic in the 1990s and a number of hydrographic sections were occupied as well. Here we use the spatially and temporally averaged velocities measured by these floats, combined with the hydrographic section data and various estimates of regional current transports from moored current meter arrays, to determine the circulation of the three major subthermocline water masses in a zonal strip across the South Atlantic between the latitudes of 19°S and 30°S. We concentrate on this region because the historical literature suggests that it is where the Deep Western Boundary Current containing NADW bifurcates. In support of this notion, we find that a net of about 5 Sv. of the 15–20 Sv that crosses 19°S does continue zonally eastward at least as far as the Mid-Atlantic Ridge. Once across the ridge it takes a circuit to the north along the ridge flanks before returning to the south in the eastern half of the Angola Basin. The data suggest that the NADW then continues on into the Indian Ocean. This scheme is discussed in the context of distributions of dissolved oxygen, silicate and salinity. In spite of the many float-years of data that were collected in the region a surprising result is that their impact on the computed solutions is quite modest. Although the focus is on the NADW we also discuss the circulation for the AAIW and AABW layers.     

The fate of dumped sediments monitored by a high-resolution multibeam echosounder system,Weser Estuary,German Bight

With the development of high-resolution multibeam echosounder systems (MBES) for surveying shallow-water areas a new tool is available to monitor rapid changes in seabed morphology as, e.g., caused by the dumping of dredge spoil in coastal waters. In this study, four data sets of repeated bathymetric surveys with a MBES were processed and analyzed. The data were collected in a 1.94-km² dumping site in the outer Weser Estuary (German Bight). Between June and December 1998, 2.6 million m³ of dredged sediment were deposited there. The bathymetric maps generated in the course of this study reveal features such as subaqueous dunes, scour holes, and mounds of dumped dredge spoil. The mean water depth decreased by about 1 m during the dumping period. Furthermore, difference grids showing changes in sediment volume allowed a calculation of the sediment budget for the monitored area. After a time period of only 5 months, 0.5 million m³ of the originally dumped 2.6 million m³ of dredge spoil had already been removed from the dumping site.     

病毒膜蛋白及其表达和纯化技术的研究进展

病毒囊膜是包膜病毒除核衣壳外的另一主要成分。它主要由多种蛋白质构成，称为膜蛋白，膜蛋白以单聚体，二聚体，三聚体，甚至更多的聚合体的形式存在，具有中和抗体，与靶细胞受体结合，凝集红细胞，诱导细胞融合等多种功能，膜蛋白可采用将其基因整合入载体，并在昆虫细胞，哺乳细胞或细菌中获得体外表达，膜蛋白可在去污剂的作用下形成蛋白质－脂－去污剂的复合物而被溶解，其中去污剂的临界微团浓度，亲水物－亲脂物平衡值，荷电、紫外穿透性等是选择合适去污剂的主要参考因子，溶解后的膜蛋白多利用聚丙烯酰胺凝胶电泳、凝胶过滤层析，离子交换层析，亲和层析，高效液相层析等技术并结合梯度离心；分级沉淀等进行分离、纯化。     

仿鱼尾潜器推进系统的水动力分析

以开发适用于小型潜器的仿生操纵与推进系统为研究背景 ,对金枪鱼的月牙形尾鳍进行水动力分析。文中将金枪鱼的尾鳍处理为在做横移和摇摆的耦合运动的同时 ,以某一匀速向前运动的月牙形刚性尾翼。计算中应用了双曲面元和压力库塔条件 ,利用面元法计算分析该三维尾翼的非定常水动力性能。探讨了前进速度、横荡和摇首的幅度、频率及其相位差对推进性能的影响     

GPS测高技术在无验潮水深测量中的应用

应用双频GPS动态后处理高精度定位技术，建立了一套完整的GPS无验潮海洋深度测量作业模式，通过海上试验与传统作业模式作了数值分析比较，结果表明，该作业模式不仅无需验潮，而且能够有效消除传统作业模式中船只动态吃水和涌浪等因素对测量成果的影响，显著提高水深测量成果的精度。     

北太平洋宽吻海豚正常生理指标数值的算法

连续5年对青岛海豚表演馆中北太平洋宽吻海豚(Tursiops gille Dall)进行现场实验和行为学研究，获取了大量的生理指标数据，运用不同的分析和计算方法对所得数据进行了筛选、剔除及处理，确立了海豚各生理指标的正常闽值和范围确定的方法，从而为准确了解海豚机体的生理状况、疾病诊断及治疗奠定了基础。     

凡纳滨对虾咸淡水养殖系统内细菌群落组成的PCR-DGGE分析

采用PCR(polym erase chain reaction)-DGGE(denaturing grad ient gel electrophoresis)及传统的微生物培养方法对凡纳滨对虾Litopenaeus vannam ei咸淡水养殖系统内各种环境的细菌群落组成进行了比较研究。传统的微生物培养计数表明,从海湾水、养殖池水到对虾粪样,细菌和弧菌的数量表现出依次增加的趋势,粪样及肠壁中弧菌的数量高出外界水环境1—4个数量级。相对于外界水环境,粪样中有很高的芽孢杆菌孢子含量,但是肠壁定植细菌中不存在芽孢杆菌(孢子)。PCR-DGGE及聚类分析结果表明,从海湾沿岸、养殖池、对虾粪便到对虾肠壁,细菌群落的多样性由高到低,无论是在哪种环境,群落的优势种都十分明显,且只有2—4种。来自同一环境各样品间的细菌群落组成非常相似,来自不同环境的样品,其细菌群落组成差别较大。聚类图上各簇的排列顺序反映了样品在取样空间分布上的毗邻次序和它们的相似程度。     

胶州湾异养细菌及大肠菌群的分布及对陆源污染的指示

2002年2月—2004年11月对胶州湾异养细菌和大肠菌群进行长期调查发现,胶州湾异养细菌丰度在105—106cells/ml之间,其中河口区域和近岸区域数量较高,其它区域相对较低。从季节上来看,夏季、秋季细菌丰度较高(分别为7.4×105、7.2×105cells/ml),冬季和春季丰度较低(分别为4.1×105cells/ml、5.9×105cells/ml),且在一年内呈现出一定的规律性波动。从季节尺度上看异养细菌丰度与温度呈现很好的正相关性(R=0.54)。对大肠菌群的研究表明,河口区域的大肠菌群数量显著高于其它区域,并且河口区域大肠菌群常年处于严重超标状态(最高为1.1×106cells/L,超过国家前三类水质标准的100倍)。根据大肠菌群的数量状况可以将胶州湾分为三个区域,即严重污染区、中度污染区和轻度污染区。该区域划分与通过营养盐对胶州湾区域的划分(富营养区、中度营养区、贫营养区)结果一致。     

台湾的弧陆碰撞研究及其地层沉积构造研究年表

对台湾及其邻近地区典型板块构造弧陆碰撞运动的研究历史沿革与代表性观点进行了较为全面的介绍;将1977年颜沧波有关台湾及其邻近地区典型板块构造弧陆碰撞运动研究的观点与30年后黄奇瑜教授的研究成果进行了对比性介绍;同时介绍了反映台湾及其邻近地区典型板块构造弧陆碰撞运动的地层沉积构造综合研究年表。