港口靠船桩工作性状计算的双参数法

港口靠船桩是承受重复性荷载或多循环荷载的一种横向承载桩。双参数法能较好地描述靠船桩 (单桩、群桩 )的桩土工作性状。用双参数法进行计算时 ,所给的地面条件必须由重复荷载或多循环荷载作用下实测出来。根据现场试桩资料 ,标定桩土参数 ,即k =mx1/n的指数l/n、桩土相对柔度系数α、桩土综合刚度EI等值 ,可以用来设计试桩附近局部地区同类条件下的其它长桩。对于没有进行试桩的某些工程 ,可以联合运用双参数法和p -y曲线法进行桩的分析     

假单胞菌(Pseudomonas sp.cn4902)磷酸甘油磷酸酯酶基因克隆与鉴定

采用随机克隆、功能筛选、逐次排除和同源比较的基因克隆新策略进行克隆假单胞菌(Pseudomonas sp．cn4902)磷酸甘油磷酸酯酶基因的研究。结果表明，该结构基因长819bp，与铜绿假单胞菌的磷酸甘油磷酸酯酶基因的核苷酸一致性达61．5％，氨基酸同源性为56．2％。该基因已输入GenBank数据库，收录号AF348165。将该基因转化大肠杆菌，受体菌在含NaCl 1．0mol／L的培养基中甘油含量升高2．9倍，最终菌浓度提高3．6倍。可见这是一个与生物耐盐性相关的主基因，以其转化、培育耐盐农作物的前景十分光明。     

A Compact Explicit Difference Scheme of High Accuracy for Extended Boussinesq Equations

Presented here is a compact explicit difference scheme of high accuracy for solving the extended Boussinesq equations.For time discretization,a three-stage explicit Runge-Kutta method with TVD property is used at predicting stage,a cubic spline function is adopted at correcting stage,which made the time discretization accuracy up to fourth order;For spatial discretization,a three-point explicit compact difference scheme with arbitrary order accuracy is employed.The extended Boussinesq equations derived by Beji and Nadaoka are solved by the proposed scheme.The numerical results agree well with the experimental data.At the same time,the comparisons of the two numerical results between the present scheme and low accuracy difference method are made,which further show the necessity of using high accuracy scheme to solve the extended Boussinesq equations.As a valid sample,the wave propagation on the rectangular step is formulated by the present scheme,the modelled results are in better agreement with the experimental data than those of Kittitanasuan.     

病毒膜蛋白及其表达和纯化技术的研究进展

病毒囊膜是包膜病毒除核衣壳外的另一主要成分。它主要由多种蛋白质构成，称为膜蛋白，膜蛋白以单聚体，二聚体，三聚体，甚至更多的聚合体的形式存在，具有中和抗体，与靶细胞受体结合，凝集红细胞，诱导细胞融合等多种功能，膜蛋白可采用将其基因整合入载体，并在昆虫细胞，哺乳细胞或细菌中获得体外表达，膜蛋白可在去污剂的作用下形成蛋白质－脂－去污剂的复合物而被溶解，其中去污剂的临界微团浓度，亲水物－亲脂物平衡值，荷电、紫外穿透性等是选择合适去污剂的主要参考因子，溶解后的膜蛋白多利用聚丙烯酰胺凝胶电泳、凝胶过滤层析，离子交换层析，亲和层析，高效液相层析等技术并结合梯度离心；分级沉淀等进行分离、纯化。     

Sea surface temperature variations in the southwestern South China Sea over the past 160 ka

Sea surface temperatures (SSTs) in the southwestern South China Sea have been reconstructed for the past 160 ka using the Uk37 paleothermometer from the core MD01-2392. The temperature differences between glacial times (MISs 6 and 2) and interglacial times (MISs 5.5 and 1) are 2.2~2.5 ℃. Younger Dryas event during the last deglaciation was documented in both the planktonic foraminiferal δ18O and SST records. After MIS 5.5, SSTs displayed a progressive cooling from 28.6 to 24.5 ℃, culminating at the LGM. During this gradual cooling period, warm events such as MISs 5.3, 5.1 and 3 were also clearly documented. By comparison of SST between the study core and Core 17954, a pattern of low or no meridional SST gradients during the interglacial periods and high meridional SST gradients during the glacial periods was exhibited. This pattern indicates the much stronger East Asian winter monsoon at the glacial than at the interglacial periods. Spectral analysis gives two prominent cycles: 41 and 23 ka, with the former more pronounced, suggesting that SSTs in the southern SCS varied in concert with high-latitude processes through the connection of East Asian winter monsoon.     

凡纳滨对虾咸淡水养殖系统内细菌群落组成的PCR-DGGE分析

采用PCR(polym erase chain reaction)-DGGE(denaturing grad ient gel electrophoresis)及传统的微生物培养方法对凡纳滨对虾Litopenaeus vannam ei咸淡水养殖系统内各种环境的细菌群落组成进行了比较研究。传统的微生物培养计数表明,从海湾水、养殖池水到对虾粪样,细菌和弧菌的数量表现出依次增加的趋势,粪样及肠壁中弧菌的数量高出外界水环境1—4个数量级。相对于外界水环境,粪样中有很高的芽孢杆菌孢子含量,但是肠壁定植细菌中不存在芽孢杆菌(孢子)。PCR-DGGE及聚类分析结果表明,从海湾沿岸、养殖池、对虾粪便到对虾肠壁,细菌群落的多样性由高到低,无论是在哪种环境,群落的优势种都十分明显,且只有2—4种。来自同一环境各样品间的细菌群落组成非常相似,来自不同环境的样品,其细菌群落组成差别较大。聚类图上各簇的排列顺序反映了样品在取样空间分布上的毗邻次序和它们的相似程度。     

清淤输泥的新方法新设备

为解决中低滩涂沉积的深厚软粘土层的输送难题,提出了一种清淤输泥的新方法,研制了能远距离输送高浓度、大粘度、低含水量的淤泥和软粘土的土方施工新设备:包括进料口、推送器、表面膜发生器、脉冲切割器、定位系统、智能控制系统、输料管道、管道浮子等。该设备具有能耗低,工效高,效益好;结构简单,故障少,运行维护方便;定位准确性高;自动化程度高等特点。可广泛应用于水利、环保、建筑工程中的淤泥和软粘土输送。     

浙江省海塘塘顶高程的确定

介绍了浙江省海塘塘顶高程确定时所涉及的波浪爬高，越浪量等的计量，同时分析了1997年后浙江省海塘塘顶高程确定的方法，由已建海塘的塘顶高程分析可见，在背水坡和塘顶有保护措施的情况下，采用允许最大越波流量来控制海塘塘顶高程，可适当降低塘身高度。     

Preliminary Study on Oil Oxidizing Degradation of Hydrocarbon

The present note describes the microbial degradation of crude oil and hydrocarbons, and discusses the microbial purification of the sea. The study shows that, (1) The ability, of Gram-negative bacteria to degrade crude oil is higher than Gram-positive bacteria. Degradation by Flavobacterium spp. and Xanthomonas spp. is higher than others; (2) The ability of dif-     

金属离子对锯缘青蟹NAGase活力的影响

本文研究了几种金属离子对锯缘青蟹（Scylla serrata）N-乙酰-β-D-氨基葡萄糖苷酶（NAGase）活力的影响．其结果表明：Li^＋、Na^＋和K^＋对酶活力没有明显影响，Mg^2＋、Ca^2＋和Ba^2＋对该酶均有激活作用，激活程度依次为Ca^2＋〉Ba^2＋〉Mg^2＋．Al^3＋、Fe^3＋、Cd^2＋、Pb^2＋和Hg^2＋对该酶具有一定的抑制作用，2．0μmol／dm^3的Hg^2＋可以使酶活力完全丧失．Co^2＋对酶的效应是先激活后抑制，Mn^2＋对酶仅有轻微的激活作用．Cd^2＋和Fe^2＋对锯缘青蟹NAGase的抑制作用都呈竞争性-反竞争性混合Ⅱ型效应，Cd^2＋的抑制常数Ki和KiS分别为23．9、5．0mmool／dm^3，Fe^3＋的抑制常数Ki和KiS分剐为395．5、135．6μmol／dm^3．Ki〉KIS说明酶一底物络合物（ES）与抑制剂的亲和力比游离酶（E）与抑制剂的亲和力大．