Morphology of Shatsky Rise oceanic plateau from high resolution bathymetry

Newly collected, high resolution multi-beam sonar data are combined with previous bathymetry data to produce an improved bathymetric map of Shatsky Rise oceanic plateau. Bathymetry data show that two massifs within Shatsky Rise are immense central volcanoes with gentle flank slopes declining from a central summit. Tamu Massif is a slightly elongated, dome-like volcanic edifice; Ori Massif is square shaped and smaller in area. Several down-to-basin normal faults are observed on the western flank of the massifs but they do not parallel the magnetic lineations, indicating that these faults are probably not related to spreading ridge faulting. Moreover, the faults are observed only on one side of the massifs, which is contrary to expectations from a mechanism of differential subsidence around the massif center. Multi-beam data show many small secondary cones with different shapes and sizes that are widely-distributed on Shatsky Rise massifs, which imply small late-stage magma sources scattered across the surface of the volcanoes in the form of lava flows or explosive volcanism. Erosional channels occur on the flanks of Shatsky Rise volcanoes due to mass wasting and display evidence of down-slope sediment movement. These channels are likely formed by sediments spalling off the edges of summit sediment cap.     

A field comparison of two methods for the determination of copper complexation: Bacterial bioassay and fixed-potential amperometry

Complexation of copper added to seawater was determined by bacterial bioassay and fixedpotential amperometry. Consistent results were obtained by these two fundamentally different methods. The results of this study support the validity of both techniques and the field applicability of fixed-potential amperometry.The intercomparison studies were performed on samples collected at the N.Y.C. sewage sludge dumpsite and in relatively unpolluted coastal waters. In this limited study, the calculated free cupric ion concentrations at ambient total copper concentrations were similar at both sites.     

Photoadaptation of carboxylating enzymes and photosynthesis during a spring bloom

Properties of the light saturation curve of photosynthesis and ribulose-1,5-bisphosphate carboxylase (RuBPC) activity are shown to change qualitatively in a natural population of marine phytoplankton during a spring bloom. Evidence is presented to show that these changes constitute photoadapative responses to increasing irradiance. As irradiance increased during the bloom, both the level of light-saturated photosynthesis (P_m) and the initial slope of the light saturation curve (α = photosynthetic efficiency) increased whether those parameters were normalized to chlorophyll a concentration (P_m^b, α^b) or to cell numbers (P_m^c, α^c). The magnitudes of these changes were such that I_k (= P_m/α, the photoadaptation parameter) did not change, but I_m, the light intensity at which photosynthesis becomes saturated, increased. RuBPC activity, both chlorophyll a (RuBPC^b) and cell number normalized (RuBPC^c), also increased during the bloom. We suggest that these adaptations were achieved by simultaneously increasing the number of photosynthetic units, proportionately decreasing the photosynthetic unit size, and increasing both the concentrations of the enzymes of the dark reactions and possibly also of photosynthetic electron transport components.We also observed diminished levels of photoinhibition in the high light adapted cells late in the bloom and have suggested that this was a consequence of the same suite of physiological changes.In situ carbon fixation per cell increased during the bloom whereas no change occurred in this parameter when normalized to chlorophyll a concentration. Although these photoadaptive responses thus permitted carbon to be fixed in situ more rapidly per cell, at a constant efficiency with respect to investment of energy in the photosynthetic apparatus, they did not result in a change in growth rate. Based on consideratios of the role of time scale in physiological adaptation, however, it is suggested that the observed alterations in photosynthesis with increasing irradiance might permit a cell to more rapidly fill an energy quota for division, possibly an advantage in a mixing environment in which energy is patchily distributed, both spatially and temporalyy.Phosphoenolpyruvate carboxylase activity when normalized to chlorophyll a (PEPC^b) did not change during the bloom while chlorophyll a normalized dark carbon fixation decreased sharply and was quantitatively small compared to PEPC^b. On this basis and considering that RuBPC^b increased during the bloom, it is suggested that, although PEPC may be involved in dark carbon fixation, its most important quantitative role is probably an indirect one in light dependent photosynthesis.We have also considered the relevance of laboratory results on photoadaptation to interpretations of field studies and have suggested that batch culture studies must be treated with caution but that turbidistat and semi-continuous methods provide reasonable simulations of natural conditions.     

The capacity of marine plankton, macrophytes and particulate matter to adsorb Cu in the presence of Mg

A complexometric titration technique was employed to measure the total capacity of a variety of marine organisms to adsorb Cu²⁺. Measured adsorption capacities were 0.22 meq g⁻¹ for phytoplankton, 0.3–1.0 meq g⁻¹ for macrophytes, 1.0–2.5 meq g⁻¹ for zooplankton and 0.3 meq g⁻¹ for suspended particulate matter. The capacity of these materials to adsorb Cu²⁺ was reduced significantly in the presence of Mg²⁺ at seawater concentrations. Competition between Mg^t2+ and Cu²⁺ for adsorption sites at pH 6 is described by an average conditional equilibrium constant of 10^3.7. This constant is such that very little Cu²⁺ may be adsorbed onto particulates and marine phytoplankton in the presence of Mg²⁺. Further, primary productivity data and estimates of the detrital carbon sedimentation in Long Island Sound suggest that the flux of particulate carbon is insufficient to remove significant amounts of Cu from the water column to sediments by adsorption mechanisms.     

Spatio-temporal variability of small copepods (especially Oithona plumifera) in shallow nearshore waters off the south coast of South Africa

Although small copepods are one of the main dietary sources for many commercially important fish, their role in the pelagic trophic dynamics has traditionally been underestimated due to the methodology commonly used in plankton sampling. Temporal variation in abundance of adults and nauplii of small copepods (particularly Oithona plumifera) in nearshore waters on the south coast of South Africa was investigated fortnightly over 14 months at site (km) and location (100 m) scales. Sampling was within <500 m of the shore, where depth was ca. 10 m, using vertical hauls of an 80-μm mesh plankton net from 1 m above the seabed to the surface. Twenty-seven adult copepod taxa were recorded, but Oithona spp. was consistently the most abundant. Taxon richness was 7–19 on each sampling occasion. There was strong temporal variation (Oithona varied between 0 and 2300 m⁻³), but much of this was short-term variability (e.g. between consecutive sampling sessions), with no seasonality or other long-term discernable patterns. There were periods of consistently low numbers, but very high numbers often followed samples with low abundances. Nor was there spatial structure at the location scale, though numbers differed between sites. Despite considerable variability at the location scale within sites, Kenton consistently showed higher densities than High Rocks. Separate analyses, with Bonferroni adjustment, showed that this difference was significant on eight out of 21 occasions for Oithona, six for other pelagic copepods and three for nauplii. This suggests that hydrodynamics favour aggregation of plankton at Kenton. A high degree of short-term variability, with a tendency for aggregation of small zooplankton at certain sites has implications for both pelagic processes and food-web links between the benthic and pelagic environments.     

Plastic particles in coastal pelagic ecosystems of the Northeast Pacific ocean

The purpose of this study was to examine the distribution, abundance and characteristics of plastic particles in plankton samples collected routinely in Northeast Pacific ecosystems, and to contribute to the development of ideas for future research into the occurrence and impact of small plastic debris in marine pelagic ecosystems. Plastic debris particles were assessed from zooplankton samples collected as part of the National Oceanic and Atmospheric Administration's (NOAA) ongoing ecosystem surveys during two research cruises in the Southeast Bering Sea in the spring and fall of 2006 and four research cruises off the U.S. west coast (primarily off southern California) in spring, summer and fall of 2006, and in January of 2007. Nets with 0.505 mm mesh were used to collect surface samples during all cruises, and sub-surface samples during the four cruises off the west coast. The 595 plankton samples processed indicate that plastic particles are widely distributed in surface waters. The proportion of surface samples from each cruise that contained particles of plastic ranged from 8.75 to 84.0%, whereas particles were recorded in sub-surface samples from only one cruise (in 28.2% of the January 2007 samples). Spatial and temporal variability was apparent in the abundance and distribution of the plastic particles and mean standardized quantities varied among cruises with ranges of 0.004-0.19 particles/m3, and 0.014-0.209 mg dry mass/m3. Off southern California, quantities for the winter cruise were significantly higher, and for the spring cruise significantly lower than for the summer and fall surveys (surface data). Differences between surface particle concentrations and mass for the Bering Sea and California coast surveys were significant for pair-wise comparisons of the spring but not the fall cruises. The particles were assigned to three plastic product types: product fragments, fishing net and line fibers, and industrial pellets; and five size categories: <1 mm, 1-2.5 mm, >2.5-5 mm, >5-10 mm, and >10 mm. Product fragments accounted for the majority of the particles, and most were less than 2.5 mm in size. The ubiquity of such particles in the survey areas and predominance of sizes <2.5 mm implies persistence in these pelagic ecosystems as a result of continuous breakdown from larger plastic debris fragments, and widespread distribution by ocean currents. Detailed investigations of the trophic ecology of individual zooplankton species, and their encounter rates with various size ranges of plastic particles in the marine pelagic environment, are required in order to understand the potential for ingestion of such debris particles by these organisms. Ongoing plankton sampling programs by marine research institutes in large marine ecosystems are good potential sources of data for continued assessment of the abundance, distribution and potential impact of small plastic debris in productive coastal pelagic zones.     

Advances in the fatigue assessment of wire ropes

This paper presents the findings from an in-depth analysis of the (axial) stiffness data recorded during tension–tension fatigue tests on wire ropes, particularly in relation to how changes in stiffness during testing relate to changes in rope strength. A linear relationship between stiffness and strength is shown to exist and a methodology presented for quantifying residual strength with applied cycles. New lower bound fatigue lines for six-strand rope and spiral strand are presented which are based on a 10% loss of strength. These new lines have the advantage of having been established using a common discard criterion for wire ropes.     

Quantum yield for the photochemical production of dissolved inorganic carbon in seawater

The direct photooxidation of coloured dissolved organic matter (CDOM) to dissolved inorganic carbon (DIC) may provide a significant sink for organic carbon in the ocean. To calculate the rate of this reaction on a global scale, it is essential to know its quantum yield, or photochemical efficiency. We have determined quantum yield spectra, φ(λ), (moles DIC/mole photons absorbed) for 14 samples of seawater from environments ranging from a turbid, eutrophic bay to the Gulf Stream. The spectra vary among locations, but can be represented quite well by three pooled spectra for zones defined by location and salinity: inshore φ(λ)=e^{−(6.66+0.0285(λ−290))}; coastal φ(λ)=e^{−(6.36+0.0140(λ−290))}; and open ocean φ(λ)=e^{−(5.53+0.00914(λ−290))}. Production efficiency increases offshore, which suggests that the most highly absorbing and quickly faded terrestrial chromophores are not those directly responsible for DIC photoproduction.     

Measurement of manganese, zinc and cadmium complexation in seawater using Chelex ion exchange equilibria

Equilibria between Chelex 100* and manganese, zinc and cadmium ions were used to determine the complexation of these trace metals in 36‰ Gulf Stream seawater at 25°C and pH 8.2. The method utilized radiotracers (⁵⁴Mn, ⁶⁵Zn, and ¹⁰⁹Cd) to quantify trace metal adsorption from trace metal-amended seawater and from seawater containing a series of ethylenediaminetetracetate (EDTA)—metal ion buffers. Results were consistent with Chelex adsorption of both trace metal ions and trace metal—EDTA chelates. Equilibrium models fitted to the data were used to establish conditional stability constants for Chelex adsorption of manganese, zinc and cadmium ions and for adsorption of EDTA-chelates. These models also yielded ratios of free metal ions to total dissolved trace metal concentrations in seawater: 10^−0.1 for manganese, 10^−0.2 for zinc, and 10^−1.5 for cadmium. Independent measurements with a cadmium ion-selective electrode also yielded a free: total cadmium ratio of 10^−1.5.