分光光度法测定海洋河弧菌510脂多糖

本文研究了直接测定海洋河弧菌 (vibrio fluvailis) 5 10脂多糖的分光光度法。利用 1,9-二甲基亚甲蓝 (DMB) ,在适当条件下同脂多糖发生染色反应 ,在 5 5 0 nm波长下 ,反应体系的吸光值同脂多糖含量呈线性相关。相关系数为 0 .9993,最低检出限为 10 μg/m L,相对标准偏差为 1.6 2 ,平均回收率为 (10 5± 2 .18) %。     

A combined numerical tidal model for the Hangzhou Bay and Qiantang River

-In order to avoid prescribing open boundary condition on the upstream side of the Hangzhou Bay, in numerical simulation of the tides and residual currents of the Bay, a 1-D model for the Qiantang River is connected to the 2-D model for the Hangzhou Bay. The harmonic constants of diurnal constituent [ (K1 O1)/2],semidiurnal constituent (M2) and shallow water constituent (M4) are obtained. The results produced by the combined model are in better agreement with the observed ones than those produced solely by the original 2-D model. The combined model gives much more reliable results for tide-induced residual water level and current.     

海洋湍流的测量及相关分析

对海洋湍流速度脉动的一维概率密度作了测量，并把测量结果与高 斯分布进行了比较。比较的结果表明，一个接近于高斯分布而另一个却差别较 大。计算到直至五阶湍流相关函数并与网格湍流进行了比较，结果都表明小时 间间隔中测量结果与网格湍流的结果近似一致，而大时间间隔中却相差很大。 以上结论说明了较长的测量时间的必要性。     

鲢鱼(Hypophthalmichthys molitrix)摄食强度和节律的实验研究

报道用室内实验方法对鲢鱼摄食强度和摄食节律的研究结果,探讨了影响它们的因子。提出鲢鱼在不同水温、不同花粉密度及不同鱼规格下的摄食量的统计分析。指出:食粒密度和水温与摄食量正相关,而鱼规格与摄食量负相关;食粒密度对摄食量影响最大(P<0.01),其次为水温;水温和食粒密度在影响摄食量的过程中可能有一定的交互作用。鲢鱼摄食强度在室内条件下也具明显的昼夜节律。在食粒规格较小、密度较低且溶氧充足条件下,鲢鱼夜晚非但不停食且出现摄食率次高峰。在本实验条件下,鲢鱼的日摄食节律与水温和光照无关,可能受饵料可得性和自身的生物钟节律等制约。     

On the long-term joint distribution of characteristic wave height and period and its application

By analysing the scatter diagrams of characteristic the wave height H and the period T on the basis of instrumental data from various ocean wave stations, we found that the conditional expectation and standard deviation of wave period for a given wave height can be better predicted by using the equations of normal linear regression rather than by those based on the log- normal law. The latter was implied in Ochi' s bivariate log-normal model(Ochi. 1978) for the long-term joint distribution of H and T. With the expectation and standard deviation predicted by the normal linear regression equations and applying proper types of distribution, we have obtained the conditional distribution of T for given H. Then combining this conditional P(T / H) with long-term marginal distribution of the wave height P(H) we establish a new parameterized model for the long-term joint distribution P(H,T). As an example of the application of the new model we give a method for estimating wave period associated with an extreme w     

山东近海牙鲆(Paralichthys olivaceus)自然和养殖群体10个微卫星基因座位的遗传多态性分析

采用微卫星遗传标记技术对山东近海牙鲆自然群体和养殖群体的遗传多样性进行了研究。实验所用牙鲆于2003年5月分别采自山东近海和青岛胶南养鱼场，各20尾，取全血或肌肉组织，以酚-仿抽提方法提取基因组DNA，利用筛选获得的10对微卫星引物进行PCR扩增，反应产物经8％非变性聚丙烯酰胺凝胶电泳分离、EB显色，用ImageMasterl D Elite(Version 3．01)软件分析电泳结果，并计算了相应的遗传学参数。结果表明，在自然和养殖群体中，10个基因座位的等位基因平均数(α)分别为6．7和6．1，每个基因座位有效等位基因数(αe)分别为1．8—6．8和2．5—6．7，群体平均杂合度(H)分别为0．8120和0．7310；两个群体间的遗传相似性系数、遗传距离和基因分化系数为0．8558、0．1557和0．0558；自然群体内每个座位上的多态信息含量(PIC)为0．59—0．84、个体识别率(DP)为0．54—0．86、非父排除率(PPE)为0．41—0．72，其累积个体识别率和非父排除率均达到0．9999，表明所选座位属中高识别力的遗传标记，可以将它们应用于今后牙鲆雌核发育群体的遗传变异分析以及进一步的遗传育种的研究中。     

潮汐分析和预报的准调和分潮方法 Ⅱ.短期观测的分析

目前,调和方法仍然是潮汐的分析和预报中的一种主要的基本方法,其关键在于求得尽可能准确的调和常数。在湖汐分析中,经常遇到的是对于中、长期(半个月以上)记录的处理,对于这样的资料,已有许多良好的分析方法可以采用。但是,有时我们也会遇到需要处理的短期观测资料,特别对于潮流,由于对它进行观测比水位观测要困难,常常不能获得较长期的记录,而需要从短期记录中分析出调和常数来。处理短期记录的方法也有不少,其中较为常用的是英国海军部的几种方法。这些方法只能处理一次或二次周日观测记录,当观测天数较多时,只能分组进行计算得到几组调和常数,然后再求平均值由于各组的天文条件的差別,调和常数的准确度常常有相当大的差异,而计算平均值时却没有加权,这就影响了结果的可靠性。本文建议的方法将可以直接分析任意多次周日观测记录,因而简化了计算并能得到更准确的结果。     

Photoinduced toxicity single and binary mixtures of four polycyclic aromatic hydrocarbons to the marine diatom Skeletonema costatum

Diatom Skeletonema costatum Cleve is one of the main predominant phytoplankton species in the Changjiang Estuary in China. In order to provide some basic information for future assessment of the potential risk on phytoplankton communities in this estuary caused by polycyclic aromatic hydrocarbons(PAHs), this alga was selected as a representative to investigate the photoinduced toxicity of PAHs, in single and mixture. Four PAHs including three-ring phenanthrene and anthracene, four-ring fluoranthene and pyrene were tested in the laboratory. The single toxicity of each PAH on this microalga was compared with and without the simulated solar UV radiation. The results showed that this microalga was sensitive to PAH's photoinduced toxicity. Ratios of the 72 h median effect concentration obtained for fluorescent and UV light tests were about 8.4 for phenanthrene, 13.0 for anthracene, 6.5 for fluoranthene, and 5.7 for pyrene, indicating that UV light enhanced the PAH toxicity to this alga significantly. Under the fluorescent radiation (lacking UV), the dose-response curves based on chemical concentrations revealed that the order of toxic strength was fluoranthene greater than pyrene greater than anthracene greater than phenanthrene; while under the UV radiation (476 μW/cm2 for UVA, 6.5 μW/cm2 for UVB) it became fluoranthene approximately equaling anthracene greater than pyrene greater than phenanthrene, indicating that the UV light also changed its relative toxicity to this alga. The photoinduced toxicity of PAHs to the marine diatom S. costatum might be a synergistic effect of photosensitization reactions (e.g., generation of single-state oxygen) and photomodification (photooxidation and/or photolysis).The combined effects of six binary mixtures on the marine diatom S. costatum were investigated using the additive-index method. Four binary-mixtures (phenanthrene plus anthracene; phenanthrene plus pyrene; anthracene plus fluoranthene; anthracene plus pyrene) were found to be synergistic joint action mode, while two binary-mixtures (phenanthrene plus fluoranthene; fluoranthene plus pyrene) displayed antagonist, revealing a complex pattern of possible interactions. The combined action mode of PAHs might be related to various factors such as the mixture compounds, mixture ratios and test conditions, etc.     

软粘土中插入式大直径薄壁圆筒结构的一种极限状态分析模型

根据插入式大直径薄壁圆筒结构的工作特点建立了其合理的破坏机制，以此为基础建立了受横向荷载作用的插入式大直径薄壁圆筒结构的上限法极限分析模型，从中可以得到作用于插入式大直径薄壁圆筒结构上的横向极限荷载近似的上限解；并根据上限法模型计算结果，提出了计算受横向荷载作用的插入式大圆筒结构侧壁极限抗力的简化修正模型，利用此修正模型，基于极限平衡法，可以求解作用于插入式大直径薄壁圆筒结构上的横向极限荷载。     

Application of LICOM to the numerical study of the water exchangebetween the South China Sea and its adjacent oceans

1 IntroductionThe South China Sea (SCS) is the largestmarginal sea in the western Pacific (see Fig. 1). It con-nects with the SCS through the Taiwan Strait, with thePacific through the Luzon Strait, with the Sulu Seathrough the Mindoro and Balabac Straits and with theJava Sea and Andaman Sea through the Sunda Shelf(For convenience, here we refer to the section at 1.5°N,Fig. 2). It is shown that the seasonal SCS circulation ismostly affected by the summer/winter monsoon, andthe no…