Expression of putative zinc-finger protein lcn61 gene in lymphocystis disease virus China （LCDV-cn） genome

An open reading frame (lcn61) of lymphocystis disease virus China (LCDV-cn), probably responsible for encoding putative zinc-finger proteins was amplified and inserted into pET24a (+) vector. Then it expressed in E. coli BL21 (DE3), and His-tag fusion protein of high yield was obtained. It was found that the fusion protein existed in E. coli mainly as inclusion bodies. The bioinformatics analysis indicates that LCN61 is C2H2 type zinc-finger protein containing four C2H2 zinc-finger motifs. This work provides a theory for functional research of lcn61 gene. Supported by High Technology Research and Development Program of China (863 Program, No. 2006AA100309)     

Recognition of Significant Surface Soil Geochemical Anomalies Via Weighted 3D Shortest-Distance Field of Subsurface Orebodies: A Case Study in the Hongtoushan Copper Mine,NE China

Natural Resources Research - Quantitative prediction of concealed mineralization is always confronted with difficulties in comprehensive analysis between 2D and 3D data and between qualitative and...     

关于辽宁地区b值方法定量预测的研究

本文利用地震学分析预报方法程式指南和“八五”攻关成果中提出的地震b值定量预测方法,对辽宁及邻区1969年以来发生的8次地震震例作了回顾性检验研究。在此基础上,根据震前b值的变化特征,总结出了可能适用于辽宁地区半年左右尺度的b值预测方法的异常羊别指标及预报规则。     

中国及邻区现代构造应力场的数值模拟

我国及邻区的现代构造应力场作为一平面应力问题用有限单元法进行了计算。将本区地壳看成是一不均匀的弹性板,根据各地区杨氏模量E、泊松比v和地壳厚度T的不同,组成12种材料区。全区被分成288个三角形单元。考虑了五种应力和位移边界条件,这些边界条件分别反映了印度洋板块、太平洋板块及菲律宾海板块对我国及邻区施加应力的相对大小。将计算得到的最大剪应力值与强震分布进行对比,将最大主压应力方向与震源机制解的最大主压应力方向进行对比,选择一种和实际符合得最好的边界条件作为可取的模式。结果表明:来自印度洋板块的作用力最大,大约是来自太平洋板块和菲律宾海板块作用力的两倍。     

Models of travel demand with endogenous preference change and heterogeneous agents

In the literatures of regional science, urban economics, and urban development planning, a working assumption is that individuals respond to incentives and regulations, given their preferences. Models for planning and policy analyses are used to consider what might occur if the incentives or regulations were different. In these models, however, preferences are usually assumed to be given and stable, and agents are usually assumed to be homogeneous. This paper focuses on the implications of making preferences in models of policy implementation endogenously determined and time varying heterogeneous agents. We consider first the recent literature on intertemporal choice and preference change, which cuts across many disciplines, and more briefly the literature on norm-regarding behavior. We then elaborate a simple model of transportation demand—from a static to a dynamic orientation, from fixed and exogenously given preferences of strictly self-regarding agents to endogenously determined and policy-induced preferences of heterogeneous agents—and illustrate its characteristics with simple numerical examples.     

基于便携式X射线荧光光谱法的深海沉积物现场成分快速检测及适用性评估

远洋粘土、硅藻软泥、铁锰结核及结壳是常见的深海沉积成因物质类型,在开展深海地质调查工作时及时获得这些物质的成分信息,对于提升海上地质调查效率和认知能力十分重要。通过便携式X射线荧光光谱法（pXRF）对菲律宾海深水区域60个样品进行分析,并结合实验室测试结果,评估pXRF能测出的24种元素含量数据的稳定性、准确性及相关性,探讨pXRF在海上地质调查工作中对于深海沉积物现场成分快速检测的适用性。通过综合对比研究发现,Ca、Cu、Fe、K、P、Pb、Sr、Zn、Zr九种元素稳定性、准确性和相关性较好,可以直接用于定性乃至定量研究工作。Al、Ba、Mn、Mo、Ni、Rb、Si、Ti、Th、V十种元素的三项指标参数等级略低,可用于含量高低判定和趋势分析等定性研究工作。Bi、Cs、Mg、Sb、Sc五种元素的测试效果较差,本法不建议使用。取样量充足且分布均匀、湿样烘干至恒量后研磨过筛、封装时充分压实平整、测试时间增长、选择合适的标准物质校正和检验、重点和异常样品增加测试次数等措施的执行有利于获得高精度的pXRF海上测试数据。未来对更多类型和数量深海样品开展pXRF现场分析,将有利于建立更精准的测试方法流程,达到现场快速揭示样品成分特征、确定底质类型及圈定矿化异常等目的,为海上关键决策的部署和实施提供参考和借鉴。     

南方公益性页岩气调查研究进展

2019—2021年期间,由中国地质调查局油气资源调查中心牵头组织实施的“南方页岩气地质调查工程”,以实现“新区、新层系、新类型、新认识”四新领域油气调查战略发现和突破,推动创建页岩气勘查开发新格局为目标,按照总体部署、分步实施、点上突破、面上评价原则,重点开展了南方复杂构造区页岩气地质调查与评价工作。圈定并优选了一批页岩气远景区和有利区,部署实施了二维地震、参数井和压裂试气工程,实现了长江上游新区新层系页岩气重大突破和发现,完成垭紫罗裂陷槽、皖江、川西南等重点地区页岩气资源潜力评价。这些成果大大提振了南方复杂构造区页岩气油气资源勘查的信心,推动了油气地质调查与科技创新的深度融合,形成了页岩气成藏理论新认识和调查评价新技术,发挥了公益性油气资源调查的引领和带动作用。     

Application of saddlepoint approximation in reliability analysis of dynamic systems

The application of the saddlepoint approximation to reliability analysis of dynamic systems is investigated. The failure event in reliability problems is formulated as the exceedance of a single performance variable over a prescribed threshold level. The saddlepoint approximation technique provides a choice to estimate the cumulative distribution function (CDF) of the performance variable. The failure probability is obtained as the value of the complement CDF at a specif ied threshold. The method requires computing the saddlepoint from a simple algebraic equation that depends on the cumulant generating function (CGF) of the performance variable. A method for calculating the saddlepoint using random samples of the performance variable is presented. The applicable region of the saddlepoint approximation is discussed in detail. A 10-story shear building model with white noise excitation illustrates the accuracy and effi ciency of the proposed methodology.     

Lateral continuity of basement seismic reflections in 15 Ma ultrafast-spreading crust at ODP Site 1256

The Ocean Drilling Program (ODP) initiated drilling at Site 1256D in the Guatemala Basin, about 1,000 km off the East Pacific Rise to penetrate plutonic rocks, anticipated to be relatively shallow in this region, formed at an ultra-fast spreading rate. IODP Expedition E312 successfully drilled into gabbros at ~1,150 m in basement. Multi-channel seismic traces show weak laterally coherent sub-basement reflections at borehole depths. Synthetic reflectivity seismograms were computed using a Ricker wavelet and impedance profiles from borehole sonic logs. These seismograms show significant sub-basement amplitude peaks. A zero-offset vertical seismic profile, shot on E312, was processed to investigate the authenticity of these reflections and their relationship to borehole geology. A dual scheme of the median filtering and F–K dip filtering was used. Tests with synthetic seismograms indicate the approach is effective at reasonable SNR levels. Downgoing energy is clearly identified but negligible upgoing energy is visible over random noise. These results indicate that lava flows and igneous contacts in upper ocean crust have significant topography on lateral scales less than the Fresnel Zone (~300 m) due to igneous and tectonic processes.     

Microbial community dynamics and biodegradation of polycyclic aromatic hydrocarbons in polluted marine sediments in Hong Kong

Dynamics of microbial community and biodegradation of polycyclic aromatic hydrocarbons (PAHs) in polluted marine sediments, artificially spiked with a mixture of PAHs (fluorene, phenanthrene, fluoranthene and pyrene), were examined for a period of 60 days. Microbial communities were characterised by bacterial counts, ester-linked fatty acid methyl ester (EL-FAME) analysis and denaturing gradient gel electrophoresis (DGGE). A noted reduction in species diversity occurred only in the high PAH level treatment at onset. Both EL-FAME and DGGE demonstrated a marked shift in microbial community, in all the PAH level treatments, afterwards, with increases in the number of fatty acid degraders, the relative abundance of fatty acid biomarkers for gram-negative bacteria and a decrease in species diversity. The shift was also accompanied by the significant decrease in PAH concentrations. By the end of the experiment, diversity indices, based on both approaches, recovered when PAH concentrations declined to their background levels, except in the high PAH level treatment.