鲤JAK2基因分子克隆及组织表达分析

采用同源克隆策略和RACE-PCR技术,克隆得到可能的鲤(Cyprinus carpio)两面神激酶2(JAK2)基因的c DNA全长序列,包括3 378 bp的开放阅读框,732 bp的5'-非编码区,529 bp的3'-非编码区,总长度达4 639bp。开放阅读框可编码1 125个氨基酸,推测分子质量和理论等电点分别为129.33 ku和6.99。同源性分析显示,克隆的鲤鱼基因与斑马鱼(Danio rerio)JAK2同源性最高,其氨基酸序列同一性和相似度分别达89%和95%。结构域预测表明,所编码氨基酸序列包含FERM、SH2及两个酪氨酸激酶结构域,这4个结构域也保守地存在于其他脊椎动物的JAK分子中。高级结构预测显示,鲤JAK2主要包括α-螺旋(α-helix)、β-折叠(β-sheet)和连接环(loop)等3类结构元件。脊椎动物JAK分子系统进化树显示,JAK1、JAK2、JAK3和TYK2等4类JAK分子分别聚类,鲤JAK2处于JAK2支系中,与所有其他的鱼类JAK2聚为一大支,并与两栖类和哺乳类组成的另一大支构成姊妹群,表明它们具有共同的祖先基因,为直系同源关系。实时荧光定量PCR检测结果表明,鲤JAK2在皮肤中表达量最高,其次是肠、血液和脑,而在肌肉、鳃、头肾、心、肝、脾中表达量较低;鲤JAK3在脾中表达量最高,在其他组织中表达量均很低,甚至未检出。     

滇东南老君山矿集区北部长田W(Sn)矿床成矿时代：来自白钨矿和萤石 Sm- Nd同位素定年的制约

滇东南是我国重要的钨锡多金属成矿区,老君山矿集区是其重要组成部分,围绕燕山期老君山复式花岗岩体,分布有都龙、南秧田、新寨大型-超大型Sn-W多金属矿床及多个中小型矿床(点)。该区地质演化复杂,Sn、W多金属可能存在多期成矿作用,包括燕山期、印支期和加里东期。由于目前该区发现的Sn-W矿化多分布于老君山岩体南、东侧,而岩体北部Sn-W成矿作用的研究相对较薄弱,特别是成矿年代学方面,多数矿床依然缺少精确同位素年代学数据,制约了该区Sn-W多金属成矿作用的认识和地质勘探的深入。长田是老君山岩体北缘代表性W (Sn)多金属矿床,区内加里东期和燕山期花岗岩均有分布,由于缺少年代学数据和地质地球化学研究,W(Sn)成矿作用的认识尚缺少实际地质地球化学依据,难以建立合理的成矿模式,制约了区内地质勘探的深入和突破。基于此,本文通过对长田W (Sn)矿床中白钨矿及共生萤石的Sm-Nd同位素定年,分别获得97 Ma和79 Ma两个年代学数据,显示该矿床钨矿化主成矿期应为燕山晚期,与老君山花岗岩活动时限一致,表明长田钨成矿作用与燕山期老君山花岗岩的侵入活动关系密切,而与加里东期岩浆活动关系不大。该成果丰富了...     

对陡坡陡崖引发地质灾害的潜在影响范围进行建模分析

陡坡陡崖作为一种特殊的地形地貌,是造成滑坡、泥石流等各种地质灾害的重要因素之一。由陡坡陡崖引发的地质灾害会破坏人造地物,对人类的生命财产安全造成严重危害。本文利用DEM数据提取了浙江省温州市的陡坡陡崖,对温州市陡坡陡崖的影响范围进行系统研究,构建了温州市陡坡陡崖的潜在滑坡方向模型,并确定其对人造地物（如道路、房屋）的影响。本文主要研究内容有以下两个：①提取陡坡陡崖并对其潜在滑坡方向进行建模;②根据影响范围提取受影响的人造地物并建立基础数据库。结果表明,温州市的陡坡陡崖数量多,分布范围广且集中,其引发的地质灾害会对人造地物造成严重的潜在影响。本文构建的温州市基础数据库可以描述陡坡陡崖潜在影响的房屋和道路的位置,确定灾害重点防治区域,为温州市的灾害防治工作提供服务。     

准噶尔盆地玛湖凹陷风城组页岩自生长英质矿物的成因机理及其储层改造意义

玛湖凹陷二叠系风城组是近几年准噶尔盆地页岩油接替的主攻领域,但其页岩成岩改造强烈,原始岩性归属尚不明确。X射线粉晶衍射测得风城组页岩黏土矿物含量普遍较低（<10%）,长英质矿物含量较高（60%~80%）。薄片分析发现风城组长英质页岩存在三种类型：① 粉砂级（4~63 μm）长石和石英碎屑含量丰富,为典型的粉砂岩;② 燧石条带和团块发育,为富硅页岩;③ 长英质基质发育,呈非碎屑状,且火山尘及火山玻璃少见,其矿物组成和形态与典型的黏土岩、粉砂岩及沉凝灰岩明显不同。背散射图像和高精度扫描电镜测得该长英质基质由碎屑和自生石英、钾长石和钠长石共同组成,可见黏土矿物、碎屑钾长石和钠长石向自生石英转化,碎屑钾长石向自生钠长石转化。通过调研全球中新生代碱湖沉积中自生硅酸盐矿物组合特征和成岩演化规律,探讨了二叠系风城组“贫”黏土矿物和“富”自生长英质矿物的原因,并提出风城组发育一类“改造长英质页岩”,主要由原始黏土和长石碎屑矿物和火山物质经过多期成岩改造而成。早期高碱（pH>9）沉积和成岩环境增加了SiO2溶解度和元素铝的活性,导致黏土矿物、泥级—细粉砂级长英质碎屑、火山灰等在入湖后发生“溶解、转化”,形成次稳定的硅酸盐矿物,如沸石和含镁蒙皂石,在经历漫长埋藏成岩后进一步转化为更为稳定的石英、钾长石和钠长石。碱湖页岩的成岩改造过程消耗了黏土矿物和火山灰,极大增加了页岩脆性,同时伴生了大量基质溶孔和晶间孔,是一类优质的页岩储层。     

基于夜光遥感的城市空间发展格局研究——以海口市为例

利用DMSP/OLP和NPP/VIIRS夜光遥感影像对海口近30年城市空间发展进行了研究,结果表明:海口城市化扩展速度在2008—2012年处于高峰期,扩展方向驱动力为西海岸、江东片区,当前多为内涵式填充发展.结合社会经济数据分析,发现固定资产投资额为促使建成区扩展的主要动力之一,该研究对海南省的城市规划建设具有重要的指导意义.     

跨平台高性能地图渲染技术研究

随着终端设备及操作系统多样化的发展,对地图的渲染性能提出了更高的要求.传统的地图渲染引擎,依赖于相应操作系统图形库,存在不能跨操作系统、渲染性能低、渲染风格不一致等问题.针对上述问题,本文提出一种跨平台的高性能地图渲染技术,采用蒙板绘制技术、纹理字典文本绘制技术,基于OpenGL(open graphics library)图形库,实现了在不同的硬件平台、不同的操作系统下,统一的高性能地图渲染引擎,保持了在不同平台下一致的地图渲染风格,性能比通用地图(MapBox)渲染引擎性能提高了1.75倍,矢量切片渲染性能为MapBox的80倍.     

台风“苏力”灾害风险与防御行为效益评估

利用2013年台风“苏力”的监测资料、台风灾情资料、2000年后福建省台风灾害数据库资料和台风“苏力”灾害防御行为效益评估网络问卷调查资料,采用相似分析法的上下限区间估算法,预评估台风“苏力”造成的受灾人口和直接经济损失,并利用台风灾害风险区划方法,对台风“苏力”进行灾害风险区划。结果表明：台风“苏力”预评估结果与实际灾情相符,台风“苏力”灾害风险分布与实际灾情分布大部分一致,风险等级高的县市,实际灾情重,高风险区的大部县市直接经济损失均为1000万元以上。应用台风灾害防御行为效益评估三级指标体系,通过调查统计分析可知,指标体系中的各级各项指数均能较好地反映和评估政府主导、部门联动和公众参与的防御行为效益,政府主导在各类减灾行为中作用最大。     

OBJECTIVE CLASSIFICATION OF THE TRACKS OF TROPICAL STORMS IN THE BAY OF BENGAL

An objective analysis of tropical cyclone tracks is performed, with which the tracks of 131 tropical storms (TSs) in 1972-2011 are separated into three types that move west-, north- and northwestward, denoted as Types A, B and C, respectively. Type A (21 TSs and 16% of total) has the origin in the southwestern Bay of Bengal, with the TS in a unimodal distribution as its seasonal feature, occurring mainly in autumn; 18 of the 21 TSs (taking up 90%) land mostly on the western Bay coast (west of 85°E); 5% of Type-A TSs attains the wind speed of >42.7 to 48.9 m/s. Type A has little or no effect on Tibet. Type B (74 TSs, 56.6% of the total) has its preferable origin in the central Bay of Bengal, with the TS in a bimodal distribution as its seasonal pattern. This type denotes the travel in the north in spring, with the landfall of 67 of the 74 TSs (accounting for 91%) mainly on the middle coast of the Bay (85° to 95°E), and 19% of the TSs reaching the wind velocity of >42.7 to 48.9 m/s, which exert great effect on Tibet and it is this TS track that gives strong precipitation on its way through this region. Type C (36 TSs, 27.5% of the total) has its main origin in the southern part of the bay, and these TSs are formed largely in autumn, moving in the northwest direction, and 23 of the 36 TSs (64%) land mostly on the western Bay coast, lasting for a longer time, with almost no impact upon Tibet.     

THE LIMITATION OF CLOUD-BASE MASS FLUX IN CUMULUS PARAMETERIZATION AND ITS APPLICATION IN A HIGH-RESOLUTION MODEL

A large area of unrealized precipitation is produced with the standard convective parameterization scheme in a high-resolution model, while subgrid-scale convection that cannot be explicitly resolved is omitted without convective parameterization. A modified version of the convection scheme with limited mass flux at cloud base is introduced into a south-China regional high-resolution model to alleviate these problems. A strong convection case and a weak convection case are selected to analyze the influence of limited cloud-base mass flux on precipitation forecast. The sensitivity of different limitation on mass flux at cloud base is also discussed. It is found that using instability energy closure for Simplified Arakawa- Schubert Scheme will produce better precipitation forecast than the primary closure based on quasi-equilibrium assumption. The influence of the convection scheme is dependent on the upper limit of mass flux at cloud base. The total rain amount is not so sensitive to the limitation of mass flux in the strong convection case as in the weak one. From the comparison of two different methods for limiting the cloud-base mass flux, it is found that shutting down the cumulus parameterization scheme completely when the cloud-base mass flux exceeds a given limitation is more suitable for the forecast of precipitation.     

A DIAGNOSTIC ANALYSIS OF AIR TEMPERATURE ANOMALY MODE OVER CHINA IN 2009/2010 WINTER BASED ON GENERALIZED EQUILIBRIUM FEEDBACK ASSESSMENT (GEFA) METHOD

By using the observed monthly mean data over 160 stations of China and NCAR/NCEP reanalysis data, the generalized equilibrium feedback assessment(GEFA) method, combined with the methods of EOF analysis, correlation and composite analysis, is used to explore the influence of different SST modes on a wintertime air temperature pattern in which it is cold in the northeast and warm in the southwest in China. The results show that the 2009/2010 winter air temperature oscillation mode between the northern and southern part of China is closely related to the corresponding sea surface temperature anomalies(SSTA) and its associated atmospheric circulation anomalies. Exhibiting warming in Northeast China and cooling in Southwest China, the mode is significantly forced by the El Nio mode and the North Atlantic SSTA mode, which have three poles. Under the influence of SSTA modes, the surface northerly flow transported cold air to North and Northeast China, resulting in low temperatures in the regions. Meanwhile, the mid-latitude westerlies intensify and the polar cold air stays in high latitudes and cannot affect the Southwest China, resulting in the warming there.