Improved form of wind wave frequency spectrum

The lower frequency part of the theoretical wind wave spectrum proposed by the authors (Wen et al. , 1988a, b,c) has been improved and the form of spectrum is appreciably simplified. In addition to the field data collected in the Bohai Sea region and used in the previous papers, those obtained in the Huanghai Sea, the East China Sea and the South China Sea have been employed so that the improved spectra can be verified on a more extensive observational basis. Computed results agree with the observations well. Further comparisons have been made between the proposed spectra and the JONSWAP spectrum. Though the two types of spectrum are close to each other in form, the former shows, as a whole, better agreement with the observation than the latter. By introducing an improved relation between the peak-ness factor and significant wave steepness, the spectrum contains only significant wave height and period as parameters. For spectra given in this form, the computed peak frequencies coincide approximately wit     

涠洲岛珊瑚礁生态系中浮游动植物与环境因子关系的初步探讨

本文根据1990年4月、10月间的理化分析数据，结合2002年9月涠洲岛珊瑚礁生态环境的调查状况，首次从珊瑚礁生态系的角度初步探讨该岛区浮游动植物与环境因子的关系。结果表明：该岛区浮游动植物的分布具有明显的区域性和季节性，既体现了珊瑚礁生态系具有较高的初级生产力特征，也体现了浮游动植物具有显著的季节交替现象。相关分析显示，水温、盐度、N，P，Si营养盐对浮游植物的种类组成及数量变动均有明显影响，但对浮游动物的影响不大；N是该生态系中浮游植物的限制因子，P限制状况只有在珊瑚礁生长带较深的海域出现，Si呈相对富足状态。     

美国的海洋科技计划

进入21世纪之际,沿海国家纷纷调整和改组本国的海洋管理机构和科研机构,制定新时期的国家海洋发展计划,为加速海洋经济发展争夺科技优势。从本期开始,本刊将陆续刊登一些国家的海洋科技计划,供读者参考。     

Application of the first internal transcribed spacer(ITS-1) of ribosomal DNA as a molecular marker to population analysis in farrer''s scallop Chlamys farreri

Sequence variation of the first internal transcribed spacer of ribosomal DNA （ ITS - 1 ） was examined and its application to the study of genetic variation was explored in four populations of farter＇ s scallop Chlamys farreri. ITS - 1 fragments, with a length of about 300 bp,of 78 individuals collected from Dalian, Qingdao, Yantai in China and Korea respectively were amplified via PCR, cloned and sequenced. Intra-genomic variation was examined by sequencing several clones of single individuals. Alignment and polymorphism analysis detected 44 haplotypes and 50 polymorphic sites which consist of 30 substitutions and 20 indels, indicating a high level of polymorphisms. Sequence analysis also showed a very low level of intra-individual variation. All these features validated the feasibility of application of ITS - 1 fragment to population analysis. Polymorphism analysis showed that the Korea sample has the richest genetic variation, followed by Yantai and Qingdao samples. AMOVA （analysis of molecular variance） showed that the majority （96.26%） of genetic variation was distributed within populations and 3.74% resulted from among populations, but with P 〈 0.05 （ = 0.042）, indicating that the populations in this study have significant divergence. This output was basically concordant with the result arising from RAPD data and different from that from mitochondrial 16S rDNA sequence data. Discussion on this inconsistency was made accordingly.     

钦州湾内湾贝类养殖海区水环境特征及营养状况初探

根据 1 998年 1 0月和 1 999年 5月的调查资料 ,阐述了钦州湾内湾水环境特征及 N/P的变化 ,以 DIN、DIP、COD和 Chl- a为富营养化指标 ,用营养状态质量指数 (NQI )分析探讨了该湾的营养状态 ,并从富营养角度讨论了该湾的污染程度     

红树植物白骨壤叶片衰老过程中叶肉细胞Ca2+水平的变化

用焦锑酸钙沉淀的电镜细胞化学方法，研究了白骨壤叶片衰老过程中叶肉细胞Ca^2 水平的变化．结果表明，在白骨壤幼叶和成熟叶叶肉细胞中，焦锑酸钙沉淀颗粒大量出现在液池和细胞间隙中，细胞壁中也可见少量沉淀，而细胞基质中则看不到焦锑酸钙沉淀．在衰老叶中，细胞基质和细胞膜上焦锑酸钙沉淀增加，而液泡和细胞间隙中的锑酸钙沉淀则显著减少，并且叶绿体外膜部分破损，结构破坏，核膜与液泡膜内部结构模糊，叶绿素含量、净光合速率、气扎手率和蒸腾速率显著下降．Ca^2 的区域性分布的变化与植物叶片衰老密切相关。     

对虾封闭式工厂化养殖水环境要素的变化特征及其相互关系

根据2002年8月7～8日对虾工厂化养成中后期A、B代表池的实测数据，分析讨论了该时期养殖水体中环境要素的变化特征及其相互关系．结果表明：研究水体水温、盐度和溶解氧含量适宜．日变化幅度不大．但pH值略为偏低，而COD值明显偏高．与该时期投饵量大，残饵及排泄物明显增加有关．但对A池来说，生物作用的影响占主导地位，而对B池而言．则以化学过程影响为主．     

Genetic relationship between Neobenedenia girellae and N. melleni inferred from 28S rRNA sequences

The fragments of 350 bp in 28S rRNA from the closely related monogenea of trematoda, Neobenedenia girellae and N. melleni are obtained by polymerase chain reaction (PCR) amplified using a couple of special primers and then sequenced. The results show that the comparison of 28S rRNA sequences, with only a base varying in 337 bp accounting for 0.3% genetic difference, from the relative species N. girellae and N. melleni parasitized on the different fishes in different farms displays that they possess a very high genetic similarity of 99.7%, higher than that of 99.41% for the single species N. melleni sampled in different areas, and the intraspecific divergence of N.melleni is 0.59%. Meanwhile, the interspecific differences between the two Neobenedenia and three Benedenia (i.e., B. lutjani, B. rohdei and B. seriolae) range from 2.08% tol 1.73%. In addition, UPGMA and MP molecular phylogenetic trees are constructed and proved to be consistent with each other. Though the morphological characteristics and the results of genetic diversity for the two Neobenedenia show a high similarity, whether they belong to a single species or not are still undefined, and the more genes of them should be further investigated, in combination with the systematical and detailed morphological study.     

杰氏风暴潮预报方法在珠海及粤西海域的应用

本文通过杰氏风暴潮预报方法对历史上给珠海和粤西海域造成较大影响的台风增水事件进行后报试验,并通过统计分析方法对原有的预报公式进行订正,总结出适合珠海及粤西海域的风暴潮预报公式.利用5a的时间对珠海及粤西海域的台风增水进行试报,结果表明预报准确率分别达95.0%和87.6%,尤其对一些增水较大、影响较严重的台风增水预报相对更加准确,预报准确率均达90.0%以上.这说明订正后的公式对珠海及粤西海域的风暴潮预报能得到较好的预报效果.     

浅谈振动沉管灌注桩施工工艺

本文介绍了沉管灌注桩施工时出现的问题以及防治措施,以满足施工要求及市场需求。