鳗弧菌(Vibrio anguillarum)M3菌株生长条件及其对蛋白酶产量的影响

采用体外测定细菌浓度、胞外产物(ECP)蛋白含量和蛋白酶活力的方法，进行了鳗弧菌M3菌株在2216E培养基中的培养条件研究。结果表明，该菌株用固体培养基培养至24h左右，可得到较高的菌体浓度、ECP蛋白含量和蛋白酶活力。采用响应面分析方法设计实验，用SAS统计软件分析数据，得到NaCl浓度、pH值和温度对菌体生长及蛋白酶产量影响的回归模型。在2216E培养基的基础上，添加不同氮源、碳源物质以及不同浓度蛋白胨进行生长研究。结果表明，胰大豆蛋白胨能促进菌体生长及ECP蛋白分泌；NH4Cl与酪蛋白水解物可抑制蛋白酶的产生；牙鲆肌肉匀浆对菌体、ECP蛋白产量和蛋白酶产生有不同程度的促进作用；培养基中蛋白胨浓度为4％时菌体量与ECP蛋白含量达最高值，在蛋白胨浓度为2％时蛋白酶分泌量已稳定；1％的葡萄糖、蔗糖、甘油均能显著地提高菌体及ECP蛋白产量，却抑制了蛋白酶的产生。     

锶诱导的氧化胁迫对叉鞭金藻(Dicrateria inornata)的影响

采用不同浓度的锶胁迫方法研究了叉鞭金藻生长、叶绿素、MDA含量及三种抗氧化酶SOD、CAT、GPX活性的变化。结果表明，在实验设计的各种Sr^2 浓度内叉鞭金藻均能生长，但Sr^2 浓度较高时生长受到不同程度的抑制，细胞数量比对照组分别减少15．9％和51．0％；叉鞭金藻中叶绿素含量随着Sr^2 浓度的升高而降低，呈负相关关系；叉鞭金藻中MDA含量在较低Sr^2 浓度下稍呈下降趋势，但与对照组相比并无显著差异，而在23．04mmol／L Sr^2 浓度时是对照的1．25倍；低Sr^2 浓度时，叉鞭金藻中蛋白质含量没有大的变化，当浓度超过5．76mmol／L时则引起蛋白质含量下降；叉鞭金藻中抗氧化酶SOD在低Sr^2 时分别降低12．5％、7．5％和9．5％，高Sr^2 时显著升高，为对照组的85．7％；CAT、GPX活性在低Sr^2 浓度时差别不大，但当浓度为23．04mmol／L时升高显著，比对照组分别增加了19．8％和74．4％。     

A Note on Soil Structure Resistance of Natural Marine Deposits

It has been well documented that natural normally-consolidated marine soils are generally subjected to the effects of soil structure. The interpretation of the resistance of soil structure is an important issue in the theory study and engineering practice of ocean engineering and geotechnical engineering. It is traditionally considered that the resistance of soil structure gradually disappears with increasing stress level when the applied stress is beyond the consolidation yield stress. In this study, however, it is found that this traditional interpretation of the resistance of soil structure can not explain the strength behavior of natural marine deposits with a normally-consolidated stress history A new interpretation of the resistance of soil structure is proposed based on the strength behavior. In the preyield state, the undrained strength of natural marine deposits is composed of two components: one developed by the applied stress and the other developed by the resistance of soil structure. When the applied stress is beyond the consolidation yield stress, the strength behavior is independent of the resistance of soil structure.     

The influence of 30~60 d oscillation on the development of the South China Sea summer monsoon

Using the National Center for Enviromental Prediction/National Center for Atmospheric Research (NCEP/NCAR) reanalysis data and NOAA satellite-obser ved outgoing long-wave radiation (OLR) data, the development of the South China Sea (SCS) summer monsoon and intraseasonal (30-60 d) oscillation (ISO) have been examined. The results show that there exists obvious interannual variability of intraseasonal oscillaiton. Using the 16 a time series of filtered OLR averaged over the SCS, an index is defined to define ““onset events““ over the SCS on the ISO time scales. Of the 16 a examined here, 10 shows a strong ISO signal in the onset of monsoon convection over the SCS. In these cases, the ISO initially suppresses the seasonal development of southwesterly and cyclonic circulation over the SCS before the ISO onset. As the ISO propagates northeastward, the low frequency cyclonic circulation anomaly occurs in the SCS and the low frequency southwesterly wind and convection over here dramatically intensify. The northeast progression of the ISO anomaleis plays a role in the initial suppression and then acceleration of the seasonal cycle of the SCS summer monsoon.     

人造海洋涌升流和海洋渔场

针对渔业资源日趋减少和鱼产品需求不断增加的矛盾，探讨了建造海底山脉、人工制造海洋涌升流、从而人工制造优良海洋渔场的可行性，分析了我国建造海底山脉的水深、海底和海流条件。介绍了建造海底山脉的建筑方法和国际上建造海底山脉的最新动态。论述了我国在200n mile以内建造海底山脉的有利条件。     

中国多金属结核开辟区间隙水中铜、锰、镍的分布规律

通过对东北太平洋海域中国多金属结核开辟区沉积物间隙水中铜、锰、镍等微量元素的详细研究表明,锰主要受沉积环境的影响,其含量的变化范围在0.16～8.61μg/dm3之间;铜和镍则主要与表层海水的初级生产力有关,研究区内间隙水中铜和镍含量的变化范围分别为0.16～20.8和0.80～3.12μg/dm3,且这些元素在沉积物—水界面处均存在最大浓度梯度.利用“Fick扩散定律”计算表明,锰在研究区主要是从上覆海水向沉积物扩散,是沉积物中锰的主要来源之一;而铜和镍则是从沉积物向上覆海水扩散,是底层海水中铜和镍的主要来源。与表层海水中铜和镍向底层海水的输送通量计算结果相比,底层海水中铜和镍的含量主要受沉积物的控制.     

冲绳海槽北部表层沉积物中底栖有孔虫分布及其与海洋环境的关系

对1999年6月采自冲绳海槽北部的62个表层沉积物样品进行了底栖有孔虫分析。研究表明，该区属种多为中外陆架和上部陆坡常见底栖有孔虫，其中Cibicides pseudoungerianus在研究区域内广泛分布。底栖有孔虫群落明显受研究区水团的制约，该区底栖有孔虫Q型因子分析反映的4个组合分别对应不同的水团影响：组合1以Cibicides pseudoungerianus、Uvigerina sp.、Ammonia ketienziensis、Textularia pseudocarinata为主，代表陆架混合水团影响环境；组合2以Uvigerina peregrina、Beregrina、Bulimina mexicana、Bulimina submarginata、Globocassidulina subglobosa为主，代表黑潮暖流中层水影响下的环境；组合3以喜营养分子Bulimina marginata和Bolivina robusta为主，代表冷涡沉积和上升流沉积区的环境；组合4以Cibicides pseudoungerianus、Globocassidulina subglobosa为主，代表了对马暖流水团影响下的环境，其中水团的氧含量水平和营养状况是影响底栖有孔虫组合变化的主要因素。底栖有孔虫的丰度从西往东依次出现两个丰度高值带，一个位于研究区西部100—140m水深的陆架区，另一个位于400—600m水深的上陆坡，后者可能反映了冲绳海槽大陆坡普遍发育的陆坡上升流现象。     

Practical implementation of Hilbert-Huang Transform algorithm

Hilbert-Huang Transform (HHT) is a newly developed powerful method for nonlinear and non-stationary time series analysis. The empirical mode decomposition is the key part of HHT, while its algorithm was protected by NASA as a US patent, which limits the wide application among the scientific community. Two approaches, mirror periodic and extrema extending methods, have been developed for handling the end effects of empirical mode decomposition. The implementation of the HHT is realized in detail to widen the application. The detailed comparison of the results from two methods with that from Huang et al. (1998, 1999), and the comparison between two methods are presented. Generally, both methods reproduce faithful results as those of Huang et al. For mirror periodic method (MPM), the data are extended once forever. Ideally, it is a way for handling the end effects of the HHT, especially for the signal that has symmetric waveform. The extrema extending method (EEM) behaves as good as MPM, and it is better t     

Molecular identification of bloom-forming species Phaeocystis globosa (Pryninesiophyta) and its dispersal based on rDNA ITS sequence analysis

The colony-forming Phaeocystis species are causative agents of dense bloom occurrences in coastal waters worldwide. It is difficult to separate them because of the different morphologies associated with their colonial stages. In this study we applied molecular approaches to analyze the genetic variation of Phaeocystis globosa and Phaeocystis pouchetii from several geographic regions, and to assist in tracing the dispersal of bloom-forming Phaeocystis species in coastal waters of China. The sequences of the internal transcribed spacers (ITS1 and ITS2) of rDNA and the 5.8S ribosomal RNA gene of Phaeocystis strains were determined. Sequence comparison shows that P.globosa was the most divergent to P. pouchetii, exhibiting sequence divergence higher than 0.08. However, lower genetic divergences existed between strains of P.globosa. The sequence comparison of the Phaeocystis rDNA ITS clearly shows that the species isolated from the southeast coast of China is identified as P.globosa rather than P. cf. pouchetii or other species. Furthermore, the significance of rDNA variation in distinct global populations of P.globosa suggested it might have had sufficient time to accumulate detectable mutations at the rDNA locus, supporting the hypothesis of ancient dispersal of P.globosa to many areas, meaning that P.globosa blooms in the coastal waters of China are endemic rather than a newly introduced species or a foreign source. Finally, based on the high divergent region of rDNA ITS, a pair of species-specific primers for P.globosa were designed, they could be useful to detect the presence of this species in mixed plankton assemblages or flagellate stages that are recognized with diffculties by means of conventional microscopy.     

东中国海环流及其季节变化的数值模拟

关于东中国海环流的研究，国内外学者已做了大量的工作。早期科学家们主要依赖于对温盐资料和少数测流资料的分析研究对渤、黄、东海的环流结构有了较系统和深入的认识。东中国海环流是由一个气旋式的“流涡”组成，东侧主要是北上的黑潮-对马暖流-黄海暖流及其延伸部分；西侧为南下的沿岸流系。黑潮对东中国海环流的影响是如此之大，以致于除了某些局部区域外，上述海域主要流系的冬、夏季分布形式比较相似而无本质上的差异(胡敦欣等，1993)。但本文所研究海域正处于世界上最显著的季风区，冬、夏季盛行风向基本相反，过渡季节(春、秋季)风向多变，风力减弱；海洋热盐结构季节变化明显(如冬季混合强，而夏季层化明显等)，这些因素都使得东中国海环流存在着较明显的季节变化。 自20世纪80年代以来，东中国海环流的数值模拟工作逐步展开，并已成为研究环流结构及其形成机制的强有力工具。但由于数值模式本身以及计算方案的缺陷(如有些学者用固定的风场、温盐场对东中国海环流进行诊断模拟等)和观测资料的不足，数值模拟的结果难以得到验证，渤、黄、东海的环流研究中仍有大量的问题存在争议，以待澄清。例如，台湾暖流的来源、流径;对马暖流的来源;夏季黄海暖流的流径以及黄海冷水团环流等均有不同的论述。对黄、东海环流季节变化的数值模拟工作也较少，多用冬、夏典型月份的风场强迫积分至稳定态，给出冬、夏季环流，这种做法值得商榷。三维环流模式很难在1个月内达到稳定态，尤其是夏季层化明显、风力减弱的情况下，非常定风场的影响更应引起人们的重视。 本文采用比较符合实际的计算方案，用年循环风场和海面热通量场为外强迫，对渤、黄、东海的环流及其季节变化进行了模拟，并对一些争议问题进行了探讨。