Trace metal composition of colloidal organic material in marine environments

Marine colloidal material (1 kDa–0.2 μm) was isolated by cross-flow ultrafiltration followed by diafiltration and freeze-drying from surface waters of the Gulf of Mexico and the Middle Atlantic Bight (MAB), as well as from estuarine waters of Galveston Bay. Elemental characterization of isolated colloidal material included organic carbon (OC) and selected trace metal (Cu, Pb, Zn, Cd, Co, Ni, Cr, Be, Fe, Al, Mn, V, Ba, and Ti) determinations. It was found that levels of these metals in marine colloids ranged from <0.1 to 50 μg/g colloidal matter, except for Fe which generally had a concentration >120 μg/g. Most metals (Cu, Pb, Zn, Ni, Al, Mn, V, and Ti) had an average concentration >1 μg/g while concentrations of Cd, Co and Be were usually <1 μg/g. Metal concentrations (μg/g) in isolated colloids were, in general, higher in Galveston Bay than in the Gulf of Mexico, suggesting either high abundance of trace metals in estuarine waters or differences in organic matter composition. Higher colloidal metal concentrations in the MAB than in the Gulf of Mexico might be due to higher terrestrial inputs in the MAB. Colloidal metal concentrations (μg/g) were generally lower than those in average soils, continental crust and suspended particles. However, metal/aluminum ratios (Me/Al) in isolated marine colloids were significantly higher than those for average soils and continental crust. Most importantly, colloids had a metal composition and metal/OC ratio (Me/C) similar to humic substances and marine plankton, suggesting that marine colloids largely originate from planktonic sources and are composed of predominately organic components. The Me/C ratios of Galveston Bay colloids followed the sequence of Cu>Ni, Cr, Zn>Mn>Co>Pb, Cd, which is similar to the Irving–Williams order except for Mn, suggesting that the interaction of metals with marine colloids is determined by the affinity of metals for specific organic ligands.     

An inventory of factors that affect polysaccharide production by Phaeocystis globosa

Phaeocystis material contains polysaccharides that are built from at least eight different monosaccharides. Differences have been reported between the carbohydrate composition of different Phaeocystis species, and also between samples taken from Phaeocystis globosa blooms in different areas. In order to elucidate factors that could play a role in determining variation in carbohydrate composition and production, a number of Phaeocystis globosa strains were studied under laboratory conditions. Although there was a clear distinction of a northern and a southern cluster in the Phaeocystis globosa strains based on RAPD analysis, the differences in the composition of the mucopolysaccharides were relatively small. The contribution of glucose, however, ranged from 7–85% of total sugars. A strain that was cultured in seawaters of diverse origin produced polysaccharides of a different composition, suggesting the effect of environmental factors. The presence of bacteria affected neither the amount, nor the composition of the carbohydrates that were produced by Phaeocystis globosa. Glucose is part of both the intracellular polysaccharide pool and of the mucopolysaccharides in the colony matrix. Using specific digestion of the intracellular chrysolaminaran by laminarinase, the distribution of polysaccharides over different pools could be assessed. During growth of an axenic, mucus-producing strain, the portion of glucose present as chrysolaminaran appeared to increase. The polyglucose that was not digested by laminarinase remains unidentified. This study shows that environmental factors rather than strain differences determine differences in the sugar composition of Phaeocystis globosa, especially with respect to the glucose content of the material. A difference in the contribution of glucose could be correlated to the portion of cells in the culture that are not in the colonies. Our study emphasises that for studying polysaccharide dynamics in Phaeocystis globosa it is important to be able to discriminate between the different polysaccharide pools. Preliminary results of an enzymatic approach were promising     

Null broadening with snapshot-deficient covariance matrices in passive sonar

Adaptive-array beamforming achieves high resolution and sidelobe suppression by producing sharp s in the adaptive beampattern. Large-aperture sonar arrays with many elements have small resolution cells; interferers may move through many resolution cells in the time required for accumulating a full-rank sample covariance matrix. This leads to "snapshot-deficient" processing. In this paper, the -broadening technique originally developed for an ideal stationary problem is extended to the snapshot-deficient problem combined with white-noise constraint (WNC) adaptive processing. Null broadening allows the strong interferers to move through resolution cells and increases the number of degrees of freedom, thereby improving the detection of weak stationary signals.     

Eastern and Western Poor Cod (Trisopterus minutus capelanus) Populations in the Mediterranean Sea: Evidence from Allozyme and Minisatellite Loci

Abstract. Nine allozyme and two minisatellite loci were used to investigate potential genetic differentiation among three samples of Mediterranean poor cod, Trisopterus minutus capelanus, from the Gulf of Lion, the Tuscan Archipelago and the Aegean Sea. Both types of markers showed consistent results, with F_ST values of 0.0262 and 0.0296 (P < 0.0015, after Bonferroni correction for multiple tests) for allozymes and minisatellites, respectively. Allele frequency heterogeneity tests between pairs of samples showed a clear separation between the two western Mediterranean samples (Gulf of Lion, Tuscan Archipelago) and the eastern one (Aegean Sea). The results indicate that at least two reproductively isolated populations of poor cod occur in the Mediterranean.     

Modelling of flow around a near-bed pipeline with a spoiler

Flow around a pipeline with and without a spoiler near a smooth wall is simulated by solving the Navier–Stokes equations. Finite-difference formulation with a second-order upwind scheme in a curvilinear coordinate system is employed. The influences of the spoiler on hydrodynamic forces, pressure distribution, vortex shedding frequency, velocity profile under the pipe, as well as shear stress on the wall are investigated. The attachment of a spoiler significantly increases drag, root-mean-square (RMS) lift, flow through the gap between the pipe and the wall and shear stress on the seabed around the pipe. The spoiler also generates a non-zero mean downward force on the pipeline, which may enhance the self-burial of the pipeline.     

Continuous Plankton Recorder sampling off the east coast of North America: history and status

The Continuous Plankton Recorder (CPR) survey has sampled plankton on 14 routes off the coasts of the northeast United States and Canada between 1959 and 2000. Six of these routes are still operating and are sampled on a monthly basis. Some 2047 CPR tows have been made to the end of 2000 and the resulting database represents the most extensive time series of marine plankton available anywhere in the northwest Atlantic. The location and time span of coverage of each route is presented. In addition selected information is presented on:

1. zooplankton abundance as departures from baselines for the northeast US continental shelf;

2. interannual variation in seasonality of Gulf of Maine phytoplankton;

3. zooplankton relationships to local hydrography of the Gulf of Maine and to the North Atlantic Oscillation;

4. time-space matrices of zooplankton abundance and anomalies southeast of New York City;

5. time series of phyto- and zooplankton on the Scotian Shelf;

6. seasonal cycles of Phytoplankton Colour and of zooplankton on the Scotian Shelf and Georges Bank, and in the Gulf of Maine; and

7. monthly abundance of zooplankton in Narragansett Bay, Rhode Island.

     

Phytoplankton and iron: validation of a global three-dimensional ocean biogeochemical model

The JGOFS program and NASA ocean-color satellites have provided a wealth of data that can be used to test and validate models of ocean biogeochemistry. A coupled three-dimensional general circulation, biogeochemical, and radiative model of the global oceans was validated using these in situ data sources and satellite data sets. Biogeochemical processes in the model were determined from the influences of circulation and turbulence dynamics, irradiance availability, and the interactions among four phytoplankton functional groups (diatoms, chlorophytes, cyanobacteria, and coccolithophores) and four nutrients (nitrate, ammonium, silica, and dissolved iron).Annual mean log-transformed dissolved iron concentrations in the model were statistically positively correlated on basin scale with observations (P<0.05) over the eight (out of 12) major oceanographic basins where data were available. The model tended to overestimate in situ observations, except in the Antarctic where a large underestimate occurred. Inadequate scavenging and excessive remineralization and/or regeneration were possible reasons for the overestimation.Basin scale model chlorophyll seasonal distributions were positively correlated with SeaWiFS chlorophyll in each of the 12 oceanographic basins (P<0.05). The global mean difference was 3.9% (model higher than SeaWiFS).The four phytoplankton groups were initialized as homogeneous and equal distributions throughout the model domain. After 26 years of simulation, they arrived at reasonable distributions throughout the global oceans: diatoms predominated high latitudes, coastal, and equatorial upwelling areas, cyanobacteria predominated the mid-ocean gyres, and chlorophytes and coccolithophores represented transitional assemblages. Seasonal patterns exhibited a range of relative responses: from a seasonal succession in the North Atlantic with coccolithophores replacing diatoms as the dominant group in mid-summer, to successional patterns with cyanobacteria replacing diatoms in mid-summer in the central North Pacific. Diatoms were associated with regions where nutrient availability was high. Cyanobacteria predominated in quiescent regions with low nutrients.While the overall patterns of phytoplankton functional group distributions exhibited broad qualitative agreement with in situ data, quantitative comparisons were mixed. Three of the four phytoplankton groups exhibited statistically significant correspondence across basins. Diatoms did not. Some basins exhibited excellent correspondence, while most showed moderate agreement, with two functional groups in agreement with data and the other two in disagreement. The results are encouraging for a first attempt at simulating functional groups in a global coupled three-dimensional model but many issues remain.     

Factors affecting spatial and temporal variability in material exchange between the Southern Everglades wetlands and Florida Bay (USA)

Physical and biological processes controlling spatial and temporal variations in material concentration and exchange between the Southern Everglades wetlands and Florida Bay were studied for 2.5 years in three of the five major creek systems draining the watershed. Daily total nitrogen (TN), and total phosphorus (TP) fluxes were measured for 2 years in Taylor River, and ten 10-day intensive studies were conducted in this creek to estimate the seasonal flux of dissolved inorganic nitrogen (N), phosphorus (P), total organic carbon (TOC), and suspended matter. Four 10-day studies were conducted simultaneously in Taylor, McCormick, and Trout Creeks to study the spatial variation in concentration and flux. The annual fluxes of TOC, TN, and TP from the Southern Everglades were estimated from regression equations. The Southern Everglades watershed, a 460-km² area that includes Taylor Slough and the area south of the C-111 canal, exported 7.1 g C m⁻², 0.46 g N m⁻², and 0.007 g P m⁻², annually. Everglades P flux is three to four orders of magnitude lower than published flux estimates from wetlands influenced by terrigenous sedimentary inputs. These low P flux values reflect both the inherently low P content of Everglades surface water and the efficiency of Everglades carbonate sediments and biota in conserving and recycling this limiting nutrient. The seasonal variation of freshwater input to the watershed was responsible for major temporal variations in N, P, and C export to Florida Bay; approximately 99% of the export occurred during the rainy season. Wind-driven forcing was most important during the later stages of the dry season when low freshwater head coincided with southerly winds, resulting in a net import of water and materials into the wetlands. We also observed an east to west decrease in TN:TP ratio from 212:1 to 127:1. Major spatial gradients in N:P ratios and nutrient concentration and flux among the creek were consistent with the westward decrease in surface water runoff from the P-limited Everglades and increased advection of relatively P-rich Gulf of Mexico (GOM) waters into Florida Bay. Comparison of measured nutrient flux from Everglades surface water inputs from this study with published estimates of other sources of nutrients to Florida Bay (i.e. atmospheric deposition, anthropogenic inputs from the Florida Keys, advection from the GOM) show that Everglades runoff represents only 2% of N inputs and 0.5% of P input to Florida Bay.     

Inter-annual variability in larval supply to populations of three invertebrate taxa in the northern California Current

We investigated sources of inter-annual variability in larval supply to crab and sea urchin populations at Bodega Head and Point Reyes in northern California. During the spring and summer upwelling seasons of the years 1992 through 1997 we monitored the weekly settlement rates of nine species of crabs and two species of sea urchins. As observed in previous studies, daily values of alongshore windstress, temperature and salinity provided evidence for the poleward flow of relatively warm, low salinity water from south of Point Reyes, an apparent retention zone, during upwelling relaxation events. In years dominated by these events (1992, 1993, 1995 and 1996) we observed that alongshore windstress, temperature and salinity were coherent and temperature was significantly correlated with cancrid crab settlement. During these years the magnitude of cancrid crab settlement and the fraction of cancrid crabs relative to other crab species settling were high. Over four years of concurrent sampling there was consistently greater cancrid crab settlement at the Point Reyes site, within the retention zone, than at Bodega Head. Settlement of non-cancrid crabs (porcellanids, grapsids, pagurids and majids) was not as closely linked to intra-annual patterns of upwelling and relaxation, possibly due to the shorter seasonal availability of larvae allowing for the influence of fewer relaxation events. Settlement of this group among years was positively correlated with environmental indicators of strong seasonal upwelling; high salinity, Bakun upwelling index and low temperature. Sea urchin settlement events were observed in June and July of 1992, 1994 and 1997 during warming periods when salinity and temperature were increasing and alongshore windstress was low. Across the six years of the study, we found that cancrid crab larvae had a more even seasonal availability than larvae of non-cancrid species, which settled in greatest numbers during the early portion of the upwelling season. Sea urchins settled in greatest numbers during the later part of the upwelling season. Together these patterns demonstrate the taxon-specific way that inter-annual variability in larval supply is forced by the coincidence of larval availability with favorable physical transport mechanisms.