3种蛋白源替代鱼粉对褐点石斑鱼幼鱼血液指标的影响

以全鱼粉饲料为对照(D1组),用豆粕替代10%、20%的鱼粉(D2、D3组),玉米蛋白粉替代10%的鱼粉(D4组),啤酒酵母替代10%的鱼粉(D5组),配制5组等氮等能饲料,研究不同蛋白源替代饲料中的鱼粉对褐点石斑鱼(Epinephelus fuscoguttatus)幼鱼血液生理生化指标的影响.结果发现:投喂56 d后,D5组终末体质量显著小于D1组和D2组(p<0.05),其他各组间差异不显著(p>0.05);D1组和D2组实验鱼体质量与D5组差异显著(p<0.05).D3组血红蛋白浓度、血栓细胞密度和平均血栓细胞体积显著低于D1组(p<0.05), D4组实验鱼嗜中性粒细胞密度和巨大不成熟细胞比例显著高于D1组(p<0.05).D3组实验鱼总蛋白含量显著低于D1、D4组(p<0.05),D3组球蛋白含量显著低于D1组(p<0.05),D3组白球蛋白比显著高于其他各组(p<0.05);D3组的血糖含量显著低于D1组(p<0.05),D2、D3、D5组的甘油三酯含量显著低于D1、D4组(p<0.05);D5组肌酐含量显著低于D1组(p<0.05).以上结果表明,酵母蛋白替代饲料中10%的鱼粉对实验鱼的生长和生理状态均产生不良影响,不适于作为褐点石斑鱼幼鱼饲料的鱼粉替代物;玉米蛋白粉和豆粕蛋白可以作为褐点石斑鱼幼鱼饲料中鱼粉的替代物,但替代比例玉米蛋白粉不宜超过10%,而豆粕蛋白的替代比例应小于20%.     

Coupled horizontal–vertical stability of bearings under dynamic loading

In this study, the coupled horizontal–vertical behavior of elastomeric bearings subjected to dynamic loading is studied in detail. Under extreme dynamic loading, elastomeric bearings exhibit unstable behavior and an instantaneous loss of horizontal stiffness that is recoverable. Building on an earlier study where the authors developed an analytical model for the horizontal behavior of bearings under dynamic loads, in this study, a new analytical model for the coupled horizontal–vertical behavior of the bearings is developed. The coupled behavior of the bearing is first studied for quasi‐static loading, and later, the behavior of the bearings under dynamic loading is studied. A clear distinction is made between different types of deformation the bearing undergoes in the vertical direction. Based on experimental results, it is observed that the behavior of the bearings under dynamic loading differs markedly from that observed under static loading. A new analytical model is proposed that can account for the coupled horizontal–vertical behavior of the bearings under dynamic loading. The proposed analytical model for predicting the post‐stability vertical behavior of the bearings is verified using experimental results. The model proposed is found to successfully predict the coupled horizontal–vertical behavior of elastomeric bearings. Copyright © 2015 John Wiley & Sons, Ltd.     

Kaolinite – alkali interaction and effects on basic properties

The influence of type and amount of clays present in soils on their properties is well understood. The clays exert their influence through large specific surface area and charges on them. Their effect is mostly exhibited through inter particle bonding and subsequent particle associations. The mineralogical influence of soils in water is well documented. However, the change in soil water system because of presence some of the contaminants can greatly influence the soil behaviour. Some of the changes are due to formation of new compounds due to interactions between the soil and pollutant. The paper reports the effect of interaction of kaolinite mineral with alkali on the index properties of soils from which the geotechnical behaviour can be understood. Detailed X-ray diffraction studies have shown that sodium aluminum silicate hydroxide hydrate (NASH) is formed by clay alkali reactions. The type and amount of formation of the compound is influenced by the concentration of alkali solution. While the compound formed is in smaller quantities with 1 N NaOH solution, significantly high quantity is formed with 4 N NaOH solution. Presence of alumina is shown to play a significant role. It was observed that the formation of sodium aluminum silicate hydroxide hydrate is reduced in the presence of alumina. Specific gravity of contaminated clay soil was reduced which confirms the formation of new compounds. Water adsorption and specific surface area of soil are also influenced due to soil alkali interaction. The changes in the free swell and index properties of soil in the presence of alkali have been explained by the changes in soil fabric and the formation of new compound.     

Development of a Label-Free Colorimetric Aptasensor with Rationally Utilized Aptamer for Rapid Detection of Okadaic Acid

Okadaic acid(OA)is a typical marine toxin with strong toxicity causing diarrheic shellfish poisoning(DSP).Aptamers show great advantages in toxin detection and attract increasing attentions in the field of food analysis.In this study,a label-free col-orimetric aptasensor was constructed for visual and rapid detection of OA in shellfish.To exploit the binding capability of the anti-OA aptamer,the inherent molecular recognition mechanism of aptamer and OA was studied,based on molecular docking,fluorescent assay,and biolayer interferometry.Consistent results showed that the stem-loop near the 3’terminal of the aptamer exhibit dominate binding capacity.Based on the revealed recognition information,the aptamer was thus rationally utilized and combined with AuNPs and cationic polymer polydiallyl dimethyl ammonium chloride(PDDA)for the development of the label-free colorimetric aptasensor,in which the 3’terminal was thoroughly exposed to OA.The aptasensor provided robust performance with a linear detection range of 100-1200 nmol L-1,a limit of detection of 41.30 nmol L-1,recovery rates of 91.6%-106.2%,as well as a high selectivity towards OA in shellfish samples.The whole detection process can be completed within 1 h.To our best knowledge,this is the first time that the anti-OA aptamer was thoroughly studied,and a label-free colorimetric aptasensor was rationally designed in this way.This study not only provides a rapid detection method for highly sensitive and specific detection of OA,but also serves as a reference for the design of efficient aptasensors in the future.     

An index for tropical temperate troughs over southern Africa

Strong cases of the tropical temperate troughs (TTT) that are responsible for the most of the summer rainfall over subtropical southern Africa are analyzed. An index for identifying the TTT is introduced for the first time using anomalies of outgoing longwave radiation (OLR) and the wind. The TTT is associated with a ridge-trough-ridge wave-like structure in the lower troposphere over southern Africa and the adjoining Indian Ocean. Therefore, the index considers physical processes that occur over southern Africa, adjoining the Atlantic and Indian Oceans to depict the variability of the TTT events. Unusually strong TTT events are identified when the standard deviations of the TTT indices defined by the OLR and wind anomalies in the selected regions are above 1.5 and 0.5 respectively. After applying this criterion and filtering out consecutive events, 55 TTT events are identified during the study period of December–January–February seasons from 1980–1981 to 2009–2010. From the composite analyses of those 55 events, it is found that the TTTs evolve with suppressed (enhanced) convection over the southwest Indian Ocean adjacent to Madagascar (southern Africa). The suppressed convection is, in turn, found to be associated with the enhanced convection around Sumatra in the southeast tropical Indian Ocean. This may explain why more TTT events occur in La Niña years as compared to El Niño years. Time evolution of the canonical TTT event shows that it starts 3 days prior to the mature phase of the event, suggesting possible predictability. After reaching a matured state, the system moves east toward the Indian Ocean and decays within the subsequent couple of days. In addition, the intertropical convergence zone (ITCZ) structure changes over Southern Africa/Madagascar during the TTT event and remains similar to climatology over other regions. The results indicate that the continental part of the ITCZ intensifies prior to the TTT event and then spreads southward following the mid-latitude influence during and after the event.     

Semi-empirical Model for Estimating the Small-Strain Shear Modulus of Unsaturated Non-plastic Sandy Soils

The shear modulus at small-strain, G _max is a maximum value of shear modulus for a given stress state and void ratio, and is a key parameter to evaluate the dynamic response of geotechnical structures. However, the laboratory testing procedures for determining G _max are time-consuming, cumbersome and require elaborate equipment especially for unsaturated soil samples. A semi-empirical model is proposed in this paper that can be used to estimate the variation of G _max with respect to matric suction for non-plastic sandy soils (i.e. I _p  = 0 %). The proposed model uses the Soil–Water Characteristic Curve (SWCC) and the shear modulus at saturation condition along with two fitting parameters ζ and ξ. The proposed model permits estimation of the variation of G _max with respect to matric suction over different zones of the SWCC (i.e. boundary effect, transition, and residual zones) for various non-plastic sandy soils. The fitting parameters ζ and ξ required for the proposed semi-empirical model can be estimated from simple relationships derived from the grain size distribution curve.     

Experimental investigation of fine sediment deposition using particle image velocimetry

In order to create natural and healthy water ecosystems, the impact of hydrological processes in ponds must be identified. Pond structure may affect water quality in many ways. The present study provides a novel insight into some of the hydrological processes as well as how to employ retentions to enhance storm water detention and manage discharge. In this research, particle image velocimetry is utilized to study the two-dimensional collecting efficiency of fine sediments by retention structures with a range of hydraulic features. To examine the hydrodynamic behavior of water mixed with fine particles, a sediment basin was designed. Fluorescent polymer particles (1–50 μm) were subsequently added to this fluid mix. This study demonstrates that gravitational force increases fine particle settlement by over 50% at greater depths. The optimum collecting efficiency of sediments at the water surface was found to be around 80%. It was also determined that flow rate and particle size are directly correlated and that inlets and outlets at higher levels impede fine particle dispersion in the water column. For these reasons, it seems that adjusting the hydraulic parameters may diminish siltation.     

城市地下孤石的地震波场响应特征及其在地震层析成像中的应用研究

城市浅层地质条件复杂,地下孤石是常见的地质灾害体.为降低城市地下空间开发过程中孤石造成的安全隐患,本文通过构建城市地下孤石的数值模型,分析地震波在含孤石介质中的传播特征,根据地震波的传播特征利用层析成像技术对孤石进行速度成像,并通过工程实例进行验证.研究表明:(1)地震波场的数值模拟是研究地球物理勘探的有效方法,能高效...     

中国地区低空急流研究进展

概述了近40年来中国地区低空急流的研究成果,重点就我国低空急流的结构特征、可能机制及其对强对流天气的影响等进行了分析和总结,包括对低空急流按所在高度和风向两种方式进行分类、低空急流的空间分布和形成原因等.最后指出了目前研究的局限性及有待于进一步研究的问题,在我国针对低空急流本身结构特征的研究相对较少,应加强这方面的研究.