Effects of temperature on cellular and biochemical parameters in the crab Carcinus aestuarii (Crustacea, Decapoda)

The effects of temperature on cellular and biochemical parameters of the crab Carcinus aestuarii were evaluated. Crabs were kept for 7 days at 4, 17 (reference value) and 30 °C (salinity of 35 psu), and total haemocyte count (THC), haemocyte volume, haemocyte proliferation, phenoloxidase (PO) activity in both haemocyte lysate (HL) and cell-free haemolymph (CFH), CFH total protein and glucose levels, superoxide dismutase (SOD) and catalase (CAT) activities in both gills and digestive gland were evaluated. The lowest and the highest temperature significantly decreased THC, whereas haemocyte volume and haemolymph glucose concentration did not differ significantly among experimental conditions. Haemolymph protein concentration significantly reduced in crabs maintained at 30 °C, when compared with that of animals kept at 4 and 17 °C. Haemocyte proliferation increased significantly in crabs kept at 4 and 30 °C, when compared with that of crabs held at 17 °C. Likewise, a significantly higher PO activity was recorded in CFH from crabs kept at 4 and 30 °C, than in control crabs. Conversely, PO activity did not vary significantly in HL. With regard to antioxidant enzyme activities, a significant decrease in CAT activity was observed in gills from crabs kept at 4 °C, when compared to that of crabs kept at 17 °C and 30 °C. Results obtained demonstrated that the highest and lowest temperature tested influenced crab biological responses, and indicated that C. aestuarii modulated its cellular and biochemical parameters (mainly haemocyte proliferation, CFH protein concentrations and CFH PO activity) in order to cope with temperature.     

The Non-Gravitational Perturbations impact on the BepiColombo Radio Science Experiment and the key rôle of the ISA accelerometer: direct solar radiation and albedo effects

The paper is focused on the estimate of the impact of the non-gravitational perturbations on the orbit of the Mercury Planetary Orbiter (MPO), one of the two spacecrafts that will be placed in orbit around the innermost planet of the solar system by the BepiColombo space mission. The key rôle of the Italian Spring Accelerometer (ISA), that has been selected by the European Space Agency (ESA) to fly on-board the MPO, is outlined. In the first part of the paper, through a numerical simulation and analysis we have estimated, over a time span of several years, the long-period behaviours of the disturbing accelerations produced by the incoming direct solar radiation pressure, and the indirect effects produced by Mercury’s albedo. The variations in the orbital parameters of the spacecraft, together with their spectral contents, have been estimated over the analysed period. The direct solar radiation pressure represents the strongest non-gravitational perturbation on the MPO in the very complex radiation environment of Mercury. The order-of-magnitude of this acceleration is quite high, about 10^?6 m/s², because of the proximity to the Sun and the large area-to-mass ratio of the spacecraft. In the second part of the paper, we concentrated upon the short-period effects of direct solar radiation pressure and Mercury’s albedo. In particular, the disturbing accelerations have been compared with the ISA measurement error and the advantages of an on-board accelerometer are highlighted with respect to the best modelling of the non-gravitational perturbations in the strong radiation environment of Mercury. The readings from ISA, with an intrinsic noise level of about $10^{-9}\,m/s^{2}/\sqrt{Hz}The paper is focused on the estimate of the impact of the non-gravitational perturbations on the orbit of the Mercury Planetary Orbiter (MPO), one of the two spacecrafts that will be placed in orbit around the innermost planet of the solar system by the BepiColombo space mission. The key r?le of the Italian Spring Accelerometer (ISA), that has been selected by the European Space Agency (ESA) to fly on-board the MPO, is outlined. In the first part of the paper, through a numerical simulation and analysis we have estimated, over a time span of several years, the long-period behaviours of the disturbing accelerations produced by the incoming direct solar radiation pressure, and the indirect effects produced by Mercury’s albedo. The variations in the orbital parameters of the spacecraft, together with their spectral contents, have been estimated over the analysed period. The direct solar radiation pressure represents the strongest non-gravitational perturbation on the MPO in the very complex radiation environment of Mercury. The order-of-magnitude of this acceleration is quite high, about 10⁻⁶ m/s², because of the proximity to the Sun and the large area-to-mass ratio of the spacecraft. In the second part of the paper, we concentrated upon the short-period effects of direct solar radiation pressure and Mercury’s albedo. In particular, the disturbing accelerations have been compared with the ISA measurement error and the advantages of an on-board accelerometer are highlighted with respect to the best modelling of the non-gravitational perturbations in the strong radiation environment of Mercury. The readings from ISA, with an intrinsic noise level of about in the frequency band of 3·10⁻⁵–10⁻¹ Hz, guarantees a very significant reduction of the non-gravitational accelerations impact on the space mission accuracy, especially of the dominant direct solar radiation pressure.     

Probabilistic seismic hazard model for Cairo,Egypt: estimates and uncertainties

A new seismic hazard model for Cairo, the capital city of Egypt is developed herein based on comprehensive consideration of uncertainties in various components of the probabilistic seismic hazard analysis. The proposed seismic hazard model is developed from an updated catalogue of historical and instrumental seismicity, geodetic strain rates derived from GPS-based velocity-field of the crust, and the geologic slip rates of active faults. The seismic source model consists of area sources and active faults characterised to forecast the seismic productivity in the region. Ground motion prediction models are selected to describe the expected ground motion at the sites of interest. The model accounts for inherent epistemic uncertainties of statistical earthquake recurrence; maximum magnitude; ground motion prediction models, and their propagation toward the obtained results. The proposed model is applied to a site-specific hazard analysis for Kottamiya, Rehab City and Zahraa-Madinat-Nasr (hereinafter referred to as Zahraa) to the East of Cairo (Egypt). The site-specific analysis accounts for the site response, through the parameterization of the sites in terms of average 30-m shear-wave velocity (Vs30). The present seismic hazard model can be considered as a reference model for earthquake risk mitigation and proper resilience planning.     

Stability of small fishing vessels in longitudinal waves

The dynamic stability of fishing vessels in longitudinal regular waves is investigated, both analytically and experimentally. In particular, the influence of stern shape on the parametric stability of fishing vessels is studied. Vessels TS and RS have very similar main characteristics, but their sterns are different. Although their linear responses are comparable, both analytical and experimental investigations indicate substantial differences in their dynamic stability in longitudinal regular waves. Strong resonances are found for the vessel with the deep transom. The analytical method takes into consideration the effects of the heave and pitch motions and wave passage and shows good agreement with experimental results. Stability limits are obtained for different conditions and are used as an aid in the discussion of the results obtained in the tests when relevant parameters are changed, such as wave amplitude and frequency, metacentric height and roll damping moment.     

Effects of 4-nonylphenol exposure in mussels (Mytilus galloprovincialis) and crabs (Carcinus aestuarii) with particular emphasis on vitellogenin induction

Since it is often difficult to estimate possible adverse effects due to contamination in selected ecosystems, multi-species biomonitoring may provide more information, taking into account different routes of exposure, ecological roles and metabolic capabilities of animals. In this context, we exposed for 7 days the mussel Mytilus galloprovincialis and the crab Carcinus aestuarii to 4-nonylphenol (NP), a well-known xenoestrogen. In mussels (0-0.2 mg NP l(-1)), we measured NP bioaccumulation in soft tissues and vitellogenin (Vg)-like protein levels in digestive glands from both males and females by the alkali-labile phosphate assay (ALP). As no reference data were available for crab exposure, the NP 96-h LC(50) value was previously determined. Then, in sublethally exposed (0-1.0 mg NP l(-1)) male crabs, NP bioaccumulation and Vg levels were measured in hemolymph, gonads and digestive gland. Bioaccumulation of NP increased from 43 to 371 microg g(-1) d.w. in mussels, and from 3.6 to 37 microg g(-1) d.w. in crabs, depending on the NP concentration in water. Dose-dependent Vg-like protein induction was observed in both species, appearing to be related to NP bioaccumulation, although a partial decrease was recorded at the highest concentration tested. A similar trend was observed in both digestive gland and gonad of exposed crabs; Vg increased to a lesser extent, although significantly, in hemolymph. Results demonstrated that NP induces Vg synthesis both in male and female mussels, as well as in male crabs. On the basis of the responsiveness of both species investigated, a multi-species approach is indicated in biomonitoring programmes.     

Polycyclic aromatic hydrocarbon pollution in native and caged mussels.

During 1999, a biological monitoring study was conducted at four sites along the Ligurian coast (Cornigliano, Voltri, Vado Ligure and Sanremo). At each site the concentration and composition of polycyclic aromatic hydrocarbons (PAH) were investigated in native and caged mussels. The mussels (Mytilus galloprovincialis), sampled in the Spring and the Autumn, showed different accumulation patterns according to the source of pollution they were exposed to. The PAH concentrations were higher in the native than in the caged mussels. The coastal sites were classified according to PAH concentrations found in mussel tissue samples: Native mussels: Vado Ligure < Voltri < San-remo < = Cornigliano, Caged mussels: Vado Ligure = Voltri = San-remo < Cornigliano. The different classification is explained by the different location of the organisms: native mussels were located near the air-water interface, while caged mussels were situated at -3 m from the water surface. The PAH concentrations in the native and caged mussels showed a similar seasonal variability, and can provide the same information about the sources of PAHs.     

An ordered probit model for seismic intensity data

Seismic intensity, measured through the Mercalli–Cancani–Sieberg (MCS) scale, provides an assessment of ground shaking level deduced from building damages, any natural environment changes and from any observed effects or feelings. Generally, moving away from the earthquake epicentre, the effects are lower but intensities may vary in space, as there could be areas that amplify or reduce the shaking depending on the earthquake source geometry, geological features and local factors. Currently, the Istituto Nazionale di Geofisica e Vulcanologia analyzes, for each seismic event, intensity data collected through the online macroseismic questionnaire available at the web-page www.haisentitoilterremoto.it. Questionnaire responses are aggregated at the municipality level and analyzed to obtain an intensity defined on an ordinal categorical scale. The main aim of this work is to model macroseismic attenuation and obtain an intensity prediction equation which describes the decay of macroseismic intensity as a function of the magnitude and distance from the hypocentre. To do this we employ an ordered probit model, assuming that the intensity response variable is related through the link probit function to some predictors. Differently from what it is commonly done in the macroseismic literature, this approach takes properly into account the qualitative and ordinal nature of the macroseismic intensity as defined on the MCS scale. Using Markov chain Monte Carlo methods, we estimate the posterior probability of the intensity at each site. Moreover, by comparing observed and estimated intensities we are able to detect anomalous areas in terms of residuals. This kind of information can be useful for a better assessment of seismic risk and for promoting effective policies to reduce major damages.     

Hydrological regimes in different slope environments and implications on rainfall thresholds triggering shallow landslides

Natural Hazards - Assessing hazard of rainfall-induced shallow landslides represents a challenge for the risk management of urbanized areas for which the setting up of early warning systems, based...