Effects of heavy metals (Pb2+ and Cd2+) on the ultrastructure, growth and pigment contents of the unicellular cyanobacterium Synechocystis sp. PCC 6803

The unicellular cyanobacterium Synechocystis sp. PCC 6803, a model organism known for its unique combination of highly desirable molecular genetic, physiological and morphological characteristics, was employed in the present study. The species was cultured in BG11 liquid medium contained various initial concentrations of Pb2+ and Cd2+ (0, 0.5, 1, 2, 4, 6 and 8 mg/L). The experiment was conducted for six days and the metal induced alterations in the ultrastructure, growth and pigment contents were assessed. Alterations in the ultrastructure of the Synechocystis sp. PCC 6803 ceils became evident with the increased (>4 mg/L Pb2+) metal concentration. The photosynthetic apparatus (thylakoid membranes) were found to be the worst affected. Deteriorated or completely destroyed thylakoid membranes have made large empty spaces in the cell interior. In addition, at the highest concentration (8 mg/L pb2+), the polyphosphate granules became more prominent both in size and number. Despite the initial slight stimulations (0.2, 3.8 and 6.5% respectively at 0.5, 1 and 2 mg/L Pb2+), both metals inhibited the growth in a dose-dependent manner as incubation progressed. Pigment contents (chlorophyll a, βcarotene and phycocyanin) were also decreased with increasing metal concentration. Cells exposed to 6 mg/L Pb2+, resulted in 36.56, 37.39 and 29.34% reductions of chlorophyll a, β carotene and phycocyanin respectively over the control. Corresponding reductions for the same Cd2+concentrations were 57.83, 48.94 and 56.90%. Lethal concentration (96 h LC50) values (3.47 mg/L Cd2+ and 12.11 mg/L Pb2+) indicated that Synechocystis sp. PCC 6803 is more vulnerable to Cd2+ than Pb2+.     

Impact of cropping pattern changes on the exploitation of water resources: A remote sensing and GIS approach

A remote sensing-based approach was applied to study the impact of changes in cropping system on the exploitation of water resources in two districts namely Ludhiana in central Punjab and Muktsar in South-Western Punjab. Rice-wheat remained dominant rotation in Ludhiana while cottonwheat rotation was replaced partially by rice-wheat in Muktsar within a span of over five years (1998–99 to 2003–04). The solo rice-wheat system in Ludhiana district has resulted in large-scale ground water exploitation as is evident from the faster decline in water table (up to 0.9m year⁻¹) and higher tube-wells density (440 per 1000 ha). As a result, nearly 60 per cent of the total area of Ludhiana district has the water table depth greater than 10m and in some blocks, it has reached to a depth of 22 m. In Muktsar district, corresponding rise in water table is 0.2m per year and tube well density is 114 per 1000 ha. Irrigation water associated with paddy cultivation in Ludhiana and adjoining areas moves laterally through the buried paleo-channels of Sutlaj in the deeper soil profile and gets accumulated in the basin lands of Muktsar and adjoining areas and causes an extra water flux and subsequent rise in water table, recorded at 3 to 7m depth. To minimize the hydrological imbalance of the state, it is suggested to diversify some of the area in the central districts from irrigation water intensive rice-wheat system to less water intensive cropping system.     

GPS工程控制网基线解算优化的探讨

对GPS基线解算的原理、影响因素和精度指标进行了分析,并结合Trimble Geomatic Office软件的基线解算实例阐述了具体的基线解算优化方法。     

Experimental studies on sloshing behavior due to horizontal movement of liquids in baffled tanks

A series of experiments have been carried out in a developed liquid sloshing setup to estimate the pressure developed on the tank walls and the free surface displacement of water from the mean static level. The square tank attached to a shaking table can be moved to and fro by a cam arrangement driven by a DC motor. Pressure and displacement studies are done on the basis of changing excitation frequency of the shaking table and fill level in the tank. Experiments were carried out without and with baffles, and the consequent changes in the parameters are observed.     

Application of a proportional-integral control for the estimation of static formation temperatures in oil wells

A strategy based on proportional-integral (PI) feedback control was applied to solve an inverse heat transfer problem for estimating static formation temperatures (SFTs) from logged temperatures in oil wells. The PI control feedbacks the error between logged and simulated temperatures during the shut-in time process, existing SFT proposal. Thus, mathematically speaking an inverse heat transfer problem was solved in this way, since SFT represents the initial conditions (which are unknown) to solve the partial differential equations governing the heat transfer process in the wellbore-formation system. The mathematical model considers transient convective heat transfer due to circulation losses to the rock surrounding a well. The methodology was tested analyzing two oil wells (MB-3007 and MB-3009) from the Gulf of Mexico and results were compared against two classic methods. The method presented in this work needs only one temperature measurement for each fixed depth to estimate the SFT.     

The aqueous geochemistry of Zr and the solubility of some Zr-bearing minerals

Literature data on the thermodynamics of complexation of Zr with inorganic species, at 25°C, have been critically reviewed. The preponderance of published complexation constants deal with F⁻ and OH⁻ ions. Stability constants for the complexation reactions are relatively independent of ionic strength and thus recomended values for each ligand type are averages of the most reliable data. Complexation constants under elevated conditions (T 250°C andP_v = P_H2O) have been predicted for various Zr complexes (F⁻, Cl⁻, SO₄²⁻ and OH⁻) using Helgeson's electrostatic approach. Predominance diagrams (calculated for simple systems with these constants) suggest that, over a wide range of pH conditions, Zr(OH)_4(aq) will dominate the aqueous geochemistry of Zr except under very high activities of competing ligands (e.g., F⁻, SO₄²⁻).The solubilities of vlasovite [Na₂ZrSi₄O₁₁] and weloganite [Sr₃Na₂Zr(CO₃)₆·3H₂O have been measured in KCI solutions (0.5–1.0 M) at 50°C. Weloganite dissolution is complicated by the predictable precipitation of strontianite (SrCO₃) whereas vlasovite dissolves incongruently. Solubility products for the dissolution of welonganite and vlasovite are determined to be −28.96±0.14 and −20.40±1.18, respectively. Concentrations of Zr up to 10⁻³ m were present in the experimental solutions; the presence of large amounts of Zr in aqueous solutions support the possibility of extensive remobilization of Zr during hydrothermal mineralization.     

塔里木盆地塔北隆起碳酸盐岩油气成藏特点

塔北隆起自早古生代以来经历了长期的挤压隆升剥蚀过程,结合寒武系膏盐的塑性上拱,形成了两种基本的碳酸盐岩圈闭类型,即风化壳潜山和内幕背斜圈闭。塔北隆起南临古生代满加尔凹陷、北临中新生代库车坳陷,具有捕获南北两侧海陆两相油气来源的条件,经历了海西期、燕山期和喜马拉雅山期3期成藏。储集空间主要靠溶蚀孔洞和裂缝。石炭系中—上泥岩段和白垩系卡普沙良群泥岩两套区域性盖层对碳酸盐岩风化壳潜山油气聚集起着至关重要的作用。不整合面和断裂是控制塔北隆起油气成藏的两个最关键的因素,不整合面既控制着岩溶储层的发育分布又是油气侧向运移的优势通道;断裂活动形成破碎带、裂缝带进而改善储层性能,同时断裂也是油气垂向运移的优势通道。     

Response of a coupled ocean-ice-atmosphere model to data assimilation in the tropical zone of the Pacific Ocean

This paper deals with numerical experiments based on the coupled ECHAM-HOPE model. The results of experiments are analyzed. The initial fields for the calculations over time periods from one month to one year are constructed based on the results of the data assimilation of temperature profiles from TOGA-TAO moorings. The perturbations of the initial fields and the propagation of these perturbations with specific computational time intervals are analyzed on the basis of the results of these experiments. It is shown that the strongest impacts of the perturbations are localized in specific regions of the World Ocean corresponding to the energetically active zones of the Earth. The mechanism of the transition of these perturbations is also studied. Different statistical properties of the ensemble of experiments are presented.     

Trophic position of Calanus finmarchicus (Copepoda, Calanoida) in the Trondheim Fjord

The trophic position of Calanus finmarchicus in the Trondheim Fjord in 2004 was determined through stable isotope analyses. Wild specimens were sampled monthly in the fjord and δ¹³C and δ¹⁵N signatures of the developmental stages from CIII to adults were measured. There were statistically significant differences in the δ¹³C and δ¹⁵N signatures of three identified groups: overwintered parental generation, developing new generation and new generation preparing for overwintering. C. finmarchicus individuals raised in a laboratory on a pure algal diet (Dunaliella tertiolecta and Isochrysis galbana) provided stable isotope signatures for purely herbivorous copepods. With these signatures as comparison, the trophic position of C. finmarchicus in the Trondheim Fjord in 2004 was determined as trophic level 2.4, thus indicating omnivory under natural conditions. Additionally, our data suggest that seasonal differences in the δ¹³C signatures of C. finmarchicus are due to the varying lipid content of the different developmental stages.