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81.
82.
One-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and high resolution two-dimensional electrophoresis (2-DE) were applied to separate protein molecules in dissolved organic matter (DOM) from oceanic waters. Results were: (1) The 2-DE distinguished a total of 412 protein spots in 10 samples from five water columns over the Pacific, although fewer than 30 proteins were resolved as bands from the identical samples by SDS-PAGE. (2) Major and ubiquitous protein bands (34 and 39 kDa proteins) on the SDS-PAGE gel were resolved into horizontally spread arrays (trains) of spots on the 2-DE gels, indicating that these bands were a mixture of protein species that have the same molecular weight (MW) but different isoelectric points (pIs). (3) Proteins that exhibited such electrophoretic patterns on the 2-DE gels were glycosylated with variable linkages between the sugar and polypeptide chains. (4) N-terminal amino-acid sequencing demonstrated that individual spots within each train of spots had identical N-terminal amino-acid sequences.The N-terminal amino-acid sequences of the 39 and 34 kDa glycoprotein spots in samples collected at different sites were also identical. Protein isoforms with the same amino-acid sequence but different glycosylation profiles, termed glycoforms, were often observed on the 2-DE gel. Thirty-one and 24 spots on the 2-DE gels were glycoforms of two glycoproteins with MWs of 39 and 34 kDa, respectively; they were one protein species. The glycoforms of the 39 kDa protein were identified as a low molecular weight alkaline phosphatase (L-AP) of Pseudomonas aeruginosa PAO1 by a homology search through five amino-acid sequence databases. The present and earlier work indicates that all identified source organisms of dissolved proteins belong to the Pseudomonas group. We propose the hypothesis that proteins associated with membrane vesicles liberated from a minor member of the bacterioplankton assemblage, the marine Pseudomonas group, are one of the important sources of dissolved proteins in oceanic waters. This hypothesis may apply to the source pathway and survival not only of proteins and also to the universally occurring bacterial peptidoglycan and lipopolysaccharide components in DOM. 相似文献
83.
Seasonal changes in nano/micro-zooplankton grazing on pico-, nano- and micro-size phytoplankton and heterotrophic nano-flagellates
(HNF) feeding on heterotrophic bacteria were quantified by the dilution technique in the surface layer off Cape Esan, southwestern
Hokkaido, Japan. Pico- and nano-size phytoplankton were major components throughout the year except in spring when a diatom
bloom was observed. Although there was little seasonal variation in bacteria and HNF biomass throughout the year, the micro-zooplankton
biomass varied appreciably with a peak in spring. Nano/micro-zooplankton grazing or feeding on pico-size chl-a and bacteria were well balanced throughout the year. However, nano-size and micro-size chl-a growth were much greater than grazing in summer. Nano/micro-zooplankton ingestion of phytoplankton was greater than their
ingestion of bacteria almost throughout the year, which suggests phytoplankton are more important as food sources of nano/micro-zooplankton
in microbial food webs off Cape Esan than bacteria off Cape Esan.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
84.
The Algal Growth Potential (AGP) of water samples collected off Gamagori in Mikawa Bay was measured from May 1978 through February 1979, and the limiting nutrient was determined using regression analysis and enrichment bioassays. The surface and bottom water samples had AGP that produced increments of chemical oxygen demand (COD) of 2.1 mg l–1 and 3.1 mg l–1, respectively, on average. These values ofCOD correspond to 46% and 97% of the average COD values of the raw water samples at the surface and bottom, respectively. Seasonal changes of AGP showed a close correlation with those of dissolved inorganic nitrogen (DIN) concentration. Enrichment bioassays showed that DIN was the most deficient nutrient. The DIN:phosphate-phosphorus (PO4
3–-P) ratios and DIN: dissolved phosphorus (DP) ratios in the water samples were below the cellular N:P ratios of the natural algal populations. These results suggest that AGP was mainly limited by DIN concentration. 相似文献
85.
Mean circulation induced over bottom topography by mesoscale variabilities in the Kuroshio Extension
Circulation could be generated over bottom topography by vertical shrinking or stretching of a water parcel, in which potential
vorticity is conserved. The water parcel moves up or down over the bottom topography yielding shrinking or stretching. In
addition to a prevailing current which advects the water parcel in one direction, an oscillatory motion can also induce shrinking
and stretching, and circulation is consequently generated over the bottom topography, once it is averaged in time. A two-layer
quasi-geostrophic model has been used to reproduce mesoscale variabilities both in and under the Kuroshio Extension around
the Shatsky Rise. A combination of TOPEX and ERS altimeters provided information on an eddy field near the sea surface, while
a data assimilation method was used to reconstruct the flow field below the main thermocline. Among various mesoscale processes
associated with the Kuroshio, it is remarkable that topographic Rossby waves trapped over the Shatsky Rise are generated by
the upper-layer mesoscale variability. A persistent anticyclonic circulation is produced on the Shatsky Rise through a water
parcel moving up and down over the bottom slope, and is consistent with the observed density anomaly in the WOA94. 相似文献
86.
Radium isotopes in coastal and open ocean surface waters of the Western North Pacific 总被引:1,自引:0,他引:1
Using manganese-impregnated fiber extraction and high-efficiency gamma counting techniques, we measured the distribution of 228Ra and 226Ra in surface waters near the coast of Japan and in the western North Pacific. There is no evidence in our data that any significant amount of 228Ra is added to open ocean surface waters from the coastal waters around Tokyo Bay. High 228Ra concentrations (> 10 dpm/103 kg), were observed along the Kuroshio Current as compared to < 2.5 dpm/103 kg between 10° and 30°N of the central gyre, and hence the major source of 228Ra in the surface water is likely to be the East Asian continental shelf zones. A simple one-dimensional eddy diffusion and advection model is used to explain the observed decrease of 228Ra from coast to the open ocean. The model results indicate two mixing regimes across the Kuroshio Current System with apparent eddy diffusion coefficients of Ky = 4 × 105 cm2 s−1 at distance y < 200 km from the coast, and Ky = 4 × 107 cm2 s−1 at y > 200 km. Along 40°N where an eastward flow of the ‘Kuroshio Extension’ prevails, an advective flow of > 0.1 knot is consistent with the observation of nearly constant 228Ra along the track.The geographical distribution pattern of 228Ra is clearly different from that of atmospherically derived 210Pb. Thus the 228Ra in surface water serves as a useful tracer that accompanies fluvially and coastally derived elements during their subsequent lateral transport toward the central gyre. 相似文献
87.
Precipitation of authigenic uranium in suboxic continental margin sediments from the Okinawa Trough 总被引:1,自引:0,他引:1
Masatoshi Yamada Zhong-Liang Wang Yoshihisa Kato 《Estuarine, Coastal and Shelf Science》2006,66(3-4):570-579
Concentrations of U and Th isotopes in Okinawa Trough and East China Sea sediment cores were determined by isotope dilution inductively coupled plasma-mass spectrometry (ID-ICP-MS) to investigate the behavior of redox sensitive uranium in suboxic hemipelagic sediments and determine their significance in oceanic uranium balance. 238U concentrations and 238U/232Th activity ratios in the East China Sea sediments showed no remarkable variation with depth. However, 238U and 238U/232Th ratios in the Okinawa Trough sediments were low in the surface oxidizing layer but increased where the suboxic condition was encountered. The distribution profiles of 230Th and 232Th concentrations were relatively constant with depth in both the Okinawa Trough and East China Sea sediment cores. These results suggested that there has been post-depositional precipitation of authigenic uranium within the suboxic Okinawa Trough sediment column. The post-depositional precipitation rates of authigenic uranium were estimated to be 47 ± 5 to >62 ± 8 ng cm−2 yr−1; these rates were comparable to those previously reported for several anoxic sediments. A mechanism controlling precipitation of uranium may be the downward diffusion of uranium U(VI), reduction to U(IV) and finally precipitation onto the solid phase. The accumulation rate of uranium for the Okinawa Trough sediments was approximately eight times higher than the world average rate reported for suboxic sediments. This removal of uranium in the oceanic budget increases the importance of the suboxic sediment sink. 相似文献
88.
Hirotaka Ito Motoki Kino Nozomu Kawakatu Naoki Isobe Shoichi Yamada 《Astrophysics and Space Science》2007,311(1-3):335-339
We investigate the total kinetic powers (L
j) and ages (t
age) of powerful jets of four FR II radio sources (Cygnus A, 3C 223, 3C 284, and 3C 219) by the detail comparison of the dynamical
model of expanding cocoons with observed ones. It is found that these sources have quite large kinetic powers with the ratio
of L
j to the Eddington luminosity (L
Edd) resides in 0.02<L
j/L
Edd<10. Reflecting the large kinetic powers, we also find that the total energy stored in the cocoon (E
c) exceed the energy derived from the minimum energy condition (E
min ): 2<E
c/E
min <160. This implies that a large amount of kinetic power is carried by invisible components such as thermal leptons (electron
and positron) and/or protons. 相似文献
89.
Hiroyuki Yamada Futoshi Nakamura Yasuharu Watanabe Marie Murakami Takeshi Nogami 《水文研究》2008,22(17):3545-3545
90.
A. M. Swinbank A. C. Edge Ian Smail J. P. Stott M. Bremer Y. Sato C. van Breukelen M. Jarvis I. Waddington L. Clewley J. Bergeron G. Cotter S. Dye J. E. Geach E. Gonzalez-Solares P. Hirst R. J. Ivison S. Rawlings C. Simpson G. P. Smith A. Verma T. Yamada 《Monthly notices of the Royal Astronomical Society》2007,379(4):1343-1351