A field-based population model for the sediment toxicity test organism Leptocheirus plumulosus: I. Model development

We describe the development of a stage-structured population matrix model for the estuarine amphipod Leptocheirus plumulosus, a sediment toxicity test organism, based on regular sampling of a natural population for approximately 2 years. Annual population dynamics were characterized by abundance peaks in the spring and fall, and low densities in the summer and winter. We use a sequence of seasonal projection matrices to capture these qualitative patterns in population dynamics. We compare two methods for estimating transition probabilities: multiple regression and truncated singular value decomposition (SVD). Overall, SVD gave more realistic parameter estimates than regression, although fecundity estimation was problematic. There were predictable trends in growth, survival and fecundity within years. The SVD-derived model provides a framework for assessing the population-level effects of contaminants on Leptocheirus.     

Growth of Five Species of Gorgonians (Sub-Class Octocorallia) in the Sedimented Waters of Singapore

Abstract. Annual growth rates of gorgonians in the Indo-Pacific region are reported ror the first tinie. A method of measuring gorgonian growth using periodic photographic monitoring that is sensitive to horizontal growth and changes in growth foci but that does not require tedious underwater measurements is applied. Growth parameters of five species of gorgonians from three Families ( Plexauridae. Melithaeidae . and Subergorgiidae ) were monitored over a period of 18 months using this method. Growth rates ranged from 2.30 cm a^-1 to 7.88 cm. a ^-1 in the highly sedimented waters of Singapore. The maximum number of branches added per year ranged from 1.7 to 14.5.     

Seasonal and spatial variability of the content of suspended organic substances in the active layer of the Black Sea

On the basis of the generalized data of multiannual observations (1985–1994), we analyze the seasonal variability of the vertical and spatial distributions and composition (C_sos, N_sos, C_sos/cha, and C/N) of suspended organic substances (SOS) in the shelf zone and in the upper active layer of the abyssal part of the Black Sea. The results of our analysis enable us to conclude that only a narrow coastal band of the shelf in the northwest and west parts of the sea suffers to an extremely pronounced anthropogenic impact, which manifests itself in a significant increase in the mass of suspended organic substances. The formation of new organic substances and, hence, the mass of suspended organic substances in these regions attain the level of eutrophic waters in the late-spring and summer periods. In the open-sea region, the anthropogenic impact is less pronounced and the spatial distribution of suspended organic substances is determined by the general dynamics of waters and the intensity of phytoplankton production. Translated by Peter V. Malyshev and Dmitry V. Malyshev     

Physical and hydrological characteristics of climatic frontal zones in the Tropical Atlantic

Climatic frontal zones are selected in the thermohaline fields of the Tropical Atlantic by analyzing the many-year-average seasonal database reduced to the nodes of a one-degree grid. We determine physical characteristics of the frontal zones, study their spatial and temporal variability, and reveal basic regularities of the appearance of frontal zones in the fields of thermohaline characteristics. Translated by Peter V. Malyshev and Dmitry V. Malyshev     

Turbulent diffusion of impurities in the ocean in the presence of internal waves

We study the influence of baroclinic tidal waves on the diffusion of an impurity spot in a continuously stratified fluid. The equation of turbulent diffusion is solved numerically by taking into account the wave currents. We establish the dependences of the characteristics of the diffusion process on parameters of baroclinic waves and the location of the impurity spot. Translated by Peter V. Malyshev and Dmitry V. Malyshev     

Effects of PCBs sorbed to algal paste and sediments on the stress protein response (HSP70 family) in the eastern oyster, Crassostrea virginica.

This study examined the stress protein response (HSP70 family) of reproductively inactive oysters fed 0.7 g algal paste containing 0, 0.35 and 3.5 micrograms polychlorinated biphenyls (PCBs) daily. A second set of treatment groups investigated the combined effect of PCBs and sediments (0.3 g sediments daily per oyster) on HSP70 response. After 8 weeks of PCB exposure, oyster tissues (mantle and gill) were sampled and analyzed for HSP70. Preliminary results did not show a significant effect in HSP70 response in oysters fed PCB sorbed to algal paste, albeit PCBs accumulated up to 1342 ng/g dry weight in the mantle, and up to 180 ng/g dry weight in gill tissues. However, the addition of sediments caused a significant increase in HSP70 levels of gills and mantle, although the mantle was less sensitive to the sediments.     

Seasonal variation in mussel Mytilus edulis digestive gland cytochrome P4501A- and 2E-immunoidentified protein levels and DNA strand breaks (Comet assay).

Mytilus edulis digestive gland microsomes were prepared from indigenous populations sampled from a clean reference site (Port Quin) and an urban-industrial contaminated site (Blackpool) in the UK. Samples were collected in March/April, May, August and December 1998. Western blot analysis was performed using polyclonal antibodies to fish CYP1A and rat CYP2E using partially purified M. edulis CYP as a positive control, to aid identification. CYP1A- and CYP2E-immunopositive protein levels showed different site-specific seasonal variation with higher levels of CYP2E determined in May (P < 0.05). At both sites, lower levels of CYP1A-immunopositive protein but not CYP2E-immunopositive protein were observed in the samples collected in December (P < 0.05). This correlated with lower levels of nuclear DNA damage (Comet assay expressed as per cent tail DNA) observed in December compared to August (P < 0.05).     

Increased potential for NAD(P)H-dependent reactive oxygen species production of hepatic subcellular fractions of fish species with in vivo exposure to contaminants.

The present study investigated the proposed involvement of contaminant-stimulated reactive oxygen species (ROS) production in disease processes in fish. NAD(P)H-dependent ROS production of subcellular fractions was determined by the iron/EDTA-mediated oxidation of 2-keto-4-methiolbutyric acid. Hepatic cytosolic NADPH-dependent and microsomal NAD(P)H-dependent ROS production were increased 51-160% (P < 0.05) in rainbow trout (Oncorhynchus mykiss) 15 weeks after a single i.p. injection of polychlorobiphenyl (PCB) (100 mg Clophen A50 kg-1 wet wt.). Hepatic microsomal NADH-dependent ROS production was 114% higher in perch (Perca fluviatilis) from PCB-contaminated Lake J?rnsj?n compared to clean Lake V?nern, Sweden. Hepatic mitochondrial NADH-dependent, cytosolic NADH-dependent and microsomal NADPH-dependent ROS production were variously elevated up to 160% in flounder (Platichthys flesus) at various sites along two pollution transects near to the ports of Rotterdam and Amsterdam, Netherlands. Overall the data indicate increased potential for ROS production in liver of fish exposed to field pollution, and support the hypothesis of oxidative stress as a mechanism of contaminant-mediated disease in fish.