Determining the correct identity of South African Marthasterias (Echinodermata: Asteroidea)

Marthasterias glacialis are found in the cool-temperate waters of the north-eastern Atlantic Ocean, in the subtropical waters of the Mediterranean Sea and along the south-western tip of Africa. The South African Marthasterias population includes two morphotypes, a smooth, spineless rarispina form and a spiny africana form, that have been described as separate species, subspecies, or forma by various authors over the past century. To test whether these two morphotypes represent separate species, and whether either, or both, are conspecific with the North-East Atlantic species, 78 Marthasterias were collected from the Cape Peninsula of South Africa. Morphological comparisons between individuals of the two forms showed no significant clustering of samples, indicating that there is no morphological separation of the two South African forms into distinct groupings. The africana and rarispina forms were also shown to be genetically indistinguishable, using a fragment of the mitochondrial cytochrome c oxidase subunit I (COI) gene and the nuclear internal transcribed spacer 1 (ITS1). In addition, the COI sequences were also compared to those from European specimens, and phylogenetic reconstruction and intra- and interspecific levels of divergence suggested that the South African specimens form a single group that is genetically distinct from the European M. glacialis. Although the allopatric distribution, high genetic divergence (more than 3% for the COI fragment) and morphological differences suggest that the South African form should be raised to species status under the name Marthasterias africana, further work must assess an independent genetic marker (nuclear) to support raising the COI clade to species level. True M. glacialis have a spine-armament pattern of a series of three or more regular spine rows down the length of each arm, whereas M. africana are either covered in many irregularly spaced spines, or have an extraordinarily bare surface with only two spine rows per arm. Marthasterias africana may also have an actinal spine simulating the presence of a third inferomarginal spine. This work tentatively resolves the taxonomic dispute, elucidates the separation and amalgamation of the two African forms and suggests a single, uniquely South African Marthasterias species that might be distinct from the north Atlantic M. glacialis, although further analyses to test reproductive isolation between the North-East Atlantic and South African forms are required.     

Determination of sulphur-bearing components in C1 and C2 carbonaceous chondrites by stepped combustion

Abstract— Ten type 1 and 2 carbonaceous chondrites have been analysed by stepped combustion to determine the nature of their sulphur-bearing components. All samples show complex release patterns which, when combined with published petrographic observations, can be interpreted in terms of sulphur derived from a mixture of at least seven components (elemental sulphur, organic sulphur, sulphide, FESON or tochilinite and sulphates: gypsum, bloedite and epsomite). Estimated concentrations of individual components show that most of the sulphur in the meteorites exists in oxidised form. The concentrations of reduced and oxidised components can be used to derive approximate oxidised/reduced sulphur ratios for the meteorites. These ratios are indicators of the extent of aqueous alteration and increase in the order CM < CI. Formation of the different alteration products by aqueous processes active on the meteorite parent body appears to be the most likely origin.     

The Kråkenes late-glacial palaeoenvironmental project

Kråkenes is the site of a small lake on the west coast of Norway that contains a long sequence of late-glacial sediments. The Younger Dryas is well represented, as a cirque glacier developed in the catchment at this time. This site offers unique opportunities to reconstruct late-glacial environments from independent sources of evidence; physical evidence (glacial geomorphology, sedimentology, palaeomagnetism, radiocarbon dating), and biological evidence from the remains of animals and plants derived from both the terrestrial and aquatic ecosystems. This report describes the background to the site, and the international multidisciplinary project to reconstruct late-glacial and early Holocene environmental and climatic changes at Kråkenes.     

Trace metals in Chesapeake Bay oysters: Intra-sample variability and its implications for biomonitoring

Previous studies have shown that metals from the Susquehanna River and the industrial complexes around Baltimore Harbor and Hampton Roads are bioaccumulated in the oysters of Chesapeake Bay. This paper reports two programs, one an intensive study of oysters from mid-bay sites, the other at Swan Point. Individual variability of metals in oyster tissue is reported and the implications of such variability in respect of sample size for monitoring programs discussed.The results suggest that a reasonable sample size for the mid-bay area is 15 individuals and, for Swan Point, 20, where both tissue metal concentration and sample variability apparently increased.     

Succession in Gulf of Mexico Cold Seep Vestimentiferan Aggregations: The Importance of Spatial Variability

Abstract. The slow rate of change in hydrocarbon seep communities on the upper ­Louisiana slope prevents the use of direct observation in studying successional trends. We used a chronosequence consisting of three presumed stages – juvenile, adult and senescent – to test a previous model which proposed that sulfide availability and vestimentiferan growth and physiological health decline over the lifespan of a vestimenti­feran aggregation. We replicated the chronosequence at two sites to simultaneously ­explore the influence of spatial heterogeneity on the characteristics of these communities. We determined environmental sulfide concentrations and vestimentiferan growth and condition in at least two vestimentiferan aggregations representative of each stage at each of these two sites. Hydrogen sulfide concentrations were highly variable both above and below the sediment's surface, and sulfide was present in high concentrations to sediment depths of 70  cm. Vestimentiferan growth and condition varied significantly on multiple spatial scales from sites separated by tens of kilometers, to aggregations separated by tens to hundreds of meters within a site, to individual vestimentiferans ­separated by tens of centimeters within an aggregation. The striking variability in both environmental sulfide and vestimentiferan growth and condition within individual ­aggregations suggests a crucial role for microhabitat variability in the persistence of vestimentiferan aggregations at these sites. Few significant successional trends in ­environmental sulfide or vestimentiferan growth and condition were found over the three stages tested.