Influence of capture stress, salinity and reproductive status on zinc associated with metallothionein-like proteins in the livers of three marine teleost species

The influence of capture stress, salinity and reproductive status on zinc associated with the metallothionein-like (MT-like) proteins in the livers of three marine teleost species was investigated. In striped mullet, Mugil cephalus L., the zinc associated with the MT-like protein, as determined by gel-permeation HPLC, increased significantly from approximately 102 to 1162 nmol zinc bound/g wet wt 7 days following capture in the field, handling, transportation to the laboratory and transfer to holding tanks. Significant increases were also observed following exposure to reduced salinity for 7 days. Smaller increases were observed in cultured red drum, Sciaenops ocellatus, livers following exposure to reduced salinity and physical trauma for 7 days. Finally, there was approximately a 2·5-fold increase in the zinc associated with the MT-like protein in livers of female spotted seatrout, Cynoscion nebulosus, collected during the reproductive season compared to regressed animals. These findings emphasize the need for establishing the natural fluctuations in MT levels as well as the response to common physical environmental variables before measurement of MT can be used reliably as a biochemical indicator of environmental contamination.     

Hydrocarbons in bivalve mollusks of Port Valdez, Alaska: Consequences of five years' permitted discharge

Petroleum hydrocarbons were investigated in Mytilus edulis and Macoma balthica at six intertidal sites in Port Valdez, Alaska after three to five years of oil terminal operation. Information about the concentrations of total hydrocarbons, unresolved complex mixture, normal alkanes, isoprenoids, olefins, hopanes and polycyclic aromatic hyrocarbons shows that petroleum accumulation in these bivalve mollusks is greatest at the Valdez municipal boat harbor and a crude oil terminal which has been permitted to discharge 170 kg oil day⁻¹. Accumulation two- to ten-fold less were observed at 3 km from the terminal and boat harbor. The temporal trends in petroleum hydrocarbon concentrations in M. edulis and M. balthica reflect the effects of selective retention and depuration of different hydrocarbon classes as well as variation in hydrocarbon sources. It is not clear whether the petroleum concentrations in Port Valdez bivalves had reached steady state by 1982, the final sampling time reported here.     

Winter-spring phytoplankton blooms in Dabob Bay, Washington

Scientific investigations in Dabob Bay, Washington State, USA, have been extensive since the early 1960s, but phytoplankton blooms have been studied mostly with regard to chlorophyll concentrations and little is known about the phytoplankton species themselves. Here we provide information on the species present, their abundances during blooms, their contribution to organic carbon concentrations and the ability of some phytoplankton species to produce toxic aldehydes that may impact metazoan grazers.Multiple blooms of phytoplankton, dominated by diatoms, occurred in the late winter-early spring period, with depth-integrated chlorophyll levels ranging from <20 to 230 mg m⁻² and peaks in February and April. The major bloom species included Skeletonema costatum, Thalassiosira spp. and Chaetoceros spp; Phaeocystis cf. pouchetii occurred in 2002 and 2004. Other taxa or groups of organisms that were sometimes abundant included unidentified small flagellates <10 μm in size and unidentified heterotrophic dinoflagellates. Large diatoms usually comprised most of the cell carbon, but a large, heterotrophic dinoflagellate, identified only as Gyrodinium “tear” because of its shape, was a major contributor to the microplankton carbon when present even in small numbers. Five Thalassiosira species and S. costatum were found to produce polyunsaturated aldehydes (PUA) that are known to affect copepod reproduction and hatching success. Our findings are similar to the few previous studies in the last four decades that included phytoplankton species and suggest long-term similarities and relative stability in the phytoplankton species present and their timing in Dabob Bay.     

Automatic Registration of TOBI Side-Scan Sonar and Multi-Beam Bathymetry Images for Improved Data Fusion

Deep towed side-scan sonar vehicles such as TOBI acquire high quality imagery of the seafloor with very high spatial resolution but poor locational accuracy. Fusion of the side-scan sonar data with bathymetry data from an independent source is often desirable to reduce ambiguity in geological interpretations, to aid in slant-range correction and to enhance seafloor representation. The main obstacle to fusion is accurate registration of the two datasets.The application of hierarchical chamfer matching to the registration of TOBI side-scan sonar images and multi-beam swath bathymetry is described. This matches low level features such as edges in the TOBI image, with corresponding features in a synthetic TOBI image created by simulating the flight of the TOBI vehicle through the bathymetry. The method is completely automatic, relatively fast and robust, and much easier than manual registration. It allows accurate positioning of the TOBI vehicle, enhancing its usefulness as a research tool. The method is illustrated by automatic registration of TOBI and multi-beam bathymetry data from the Mid-Atlantic Ridge.     

Open sea berths for liquid bulk carriers

The transfer of liquid bulk through flexible transfer lines can be accomplished despite relatively large ship motions. For this reason, open sea berth terminals have replaced conventional harbors in many places. The thorough understanding of moored ships dynamics when subjected to waves, wind and currents, combined with the use of flexible dolphins, flexible fenders, asymmetric constant-tension winches and nylon tails permit us to design open sea berth terminals for relatively exposed areas. The design of an integrated system of marine sensors to continuously monitor all the critical elements-ship motion and mooring and fender forces-will enhance the operational procedure whilst insuring safe operations. A berth code can then be established for insuring the maximum efficiency of the terminal on the basis of the measurement of these critical elements.     

Post-Miocene stratigraphy and depositional environments of valley-fill sequences at the mouth of Tampa Bay, Florida

Post-Miocene sea-level low stands allowed rivers and karst processes to incise the exposed carbonate platform along the Gulf Coast of Florida. Few Miocene to mid-Pleistocene deposits survived erosion along the present coast except within incised valleys. Since their formation, these valleys have been filled and incised multiple times in response to sea-level changes. The thick sedimentary sequences underlying the mouth of Tampa Bay have been recorded as a range of depositional environments and multiple sea-level incursions and excursions during pre-Holocene time and subsequent to the accumulation of the Miocene carbonate sequences. Sediment analysis of cores collected from a north–south transect across the mouth of Tampa Bay has enabled the identification of lithofacies, ranging from well-sorted, quartz sand to dense, fossiliferous, phosphatic grainstone. These facies were deposited in freshwater, estuarine, and shallow, open marine environments. As a result of channel development and migration within the paleovalley, and cut-and-fill associated with individual transgressions and regressions, correlation of the lithofacies does not extend across the entire transect. Fining-upward sequences truncated by tidal ravinement surfaces that extend throughout the paleovalley can, however, be identified. Age determinations based on 14-C analysis, amino-acid racemization, and strontium isotope analysis dating of numerous samples yield ages of Miocene, Pliocene, early Pleistocene, and late Pleistocene, as well as Holocene for sequences that accumulated and were preserved in this valley-fill complex. Numerous inconsistencies in the stratigraphic organization of the age determinations indicate that there are bad dates, considerable reworking of shells that were dated, or both. For this reason as well as the lack of detailed correlation among the three relatively complete cores, it is not possible to place these strata in a sequence stratigraphic framework.     

东海黑潮及琉球群岛以东海流研究进展

东海是西太平洋的一个边缘海,它西部有广阔的大陆架,东面以九州岛、琉球群岛(由奄美诸岛、冲绳诸岛、先岛诸岛组成)和台湾岛连线为界,具有较深的海槽。黑潮是一支高温、高盐、高流速的西边界流,它起源于菲律宾以东海域、流经台湾东岸进入东海,沿东海大陆架外缘向东北向流动，穿越吐噶喇海峡返回太平洋。通常的把自台湾东北端起至吐噶喇海峡一段称为东海黑潮。此外,在琉球群岛以东海域也常年存在一支稳定的西边界流,称为琉球海流。 对于东海黑潮和琉球海流的硏究,主要起步于20世纪80年代,通过进行中日联合调查研究、中日副热带环流调查研究等,取得了许多重要成果,苏纪兰(1994)、袁耀初(2000，Yuan,2006)、管秉贤(2002)等人都做过评述。本文基于以上研究评述,主要叙述200年以来对东海黑潮及琉球海流的硏究新进展。     

Geoid height anomalies over the Cape Verde Rise

Geoid height anomalies, as determined by satellite altimetry, suggest that the Cape Verde Rise is in local isostatic equilibrium, supported by a low-density root of altered lithosphere. A depth anomaly map shows the Cape Verde Rise to be approximately 1600 km wide and 2km high. Removal of a quadratic surface from the observed geoid heights leaves a residual positive anomaly with the same shape as the rise and an amplitude of about 8 m. The ratio of residual geoid height anomaly to depth anomaly is consistent with an isostatic root only 40 km deep on average.     

Spatial heterogeneity of epibenthos on artificial reefs: fouling communities in the early stages of colonization on an East Australian shipwreck

Artificial reefs are spatially complex habitats and serve as good model systems to study patterns of community succession and the response of epibiota to environmental clines over small spatial scales. Here, we quantified spatial heterogeneity in community composition and diversity of fouling communities across a number of environmental gradients that included water depth, surface orientation of habitats, exposure to currents, and shelter. Assemblage structure was quantified by spatially replicated photo transects on a recently scuttled large navy ship off the East Australian coast, lying in 27 m of water. A rich assemblage of epifauna had colonized the wreck within a year, dominated by barnacles, sponges and bryozoans. Community structure varied significantly over small spatial scales of meters to tens of meters. Depth, surface orientation and exposure were the major environmental drivers. Assemblages were substantially less diverse and abundant on the deepest (23 m near the seafloor) part of the hull with residual antifouling paint, on sheltered surfaces inside the wreck, and on the sediment‐laden horizontal surfaces. Overall, the wrecks’ habitat complexity corresponds with small‐scale heterogeneity in the fouling communities. This study supports the notion that wrecks enhance local diversity and biomass within the habitat mosaic of their location, and habitat complexity may be an important mechanism for this, as demonstrated by the large spatial variability in the assemblages documented here.