Ubiquitination and carbonylation as markers of oxidative-stress in Ruditapes decussatus.

Environmental pollutants, such as metals, are widespread in aquatic environments and can lead to the formation of reactive oxygen species (ROS). ROS are highly toxic in marine species since they can cause serious reversible and irreversible changes in proteins including ubiquitination and modifications such as carbonylation. This study aimed to confirm the potential of ubiquitination and carbonylation as markers of oxidative stress in the clam Ruditapes decussatus (Veneroida, Veneridae) exposed to cadmium (40 microg/L). After 21 days of exposure clams were dissected into gills and digestive gland. Cytosolic proteins of both tissues were separated by two-dimensional electrophoresis (2-D SDS-PAGE) and analysed by immunobloting. Higher ubiquitination and carbonylation levels were in digestive gland of contaminated organisms. These results confirm the potential of ubiquitination and carbonylation as a sensitive and specific marker of oxidative stress in marine bivalves. In this approach, changes in protein structure provide options for affinity selection of sub-proteomes for 2D SDS-PAGE, simplifying the detection of protein biomarkers using proteomic approach.     

Changes in climate extremes, variability and signature on sub-Antarctic Marion Island

The ecological consequences of climate change are determined by many climate parameters, not just by the commonly investigated changes in mean temperature and rainfall. More comprehensive studies, including analyses of climate variability, extremes and aggregate changes in the climate system, can improve the understanding of the nature, and therefore possible consequences, of recent changes in climate. Here climate trends on the sub-Antarctic Marion Island are documented (between 1949 and 2003) in more detail than previously. Significant trends in biologically-relevant, and previously unexplored, parameters were observed, and the potential ecological consequences of these changes discussed. For example, the decline in precipitation experienced on the island comprises a trend for longer dry spells punctuated by fewer and smaller precipitation events. This more detailed understanding of the island’s drying trends enables more accurate predictions about its impacts, including, for example, particularly severe effects on plant species growing in soils with poor water-holding capacity. Therefore, in addition to changes in average conditions, more inclusive climate analyses should also examine trends in climatic variability and extremes, for individual climate parameters as well as for the climate system as a whole.     

Petrogenesis of picrite and associated basalts from the southern Mid-Atlantic Ridge

Basalts dredged from the south wall of a fracture zone transecting the southern Mid-Atlantic Ridge (SMAR) at 54° S are unusual in that they include a suite of highly olivine phyric basalts, sampled along with more normal sparsely plagioclase phyric basalts, and a highly plagioclase phyric basalt. Four basalt types (olivine phyric, sparsely plagioclase phyric, evolved sparsely plagioclase phyric and highly plagioclase phyric) are readily distinguished on the basis of petrography, mineralogy and bulk composition. They range from primitive to evolved, with the olivine phyric basalts having elevated MgO (up to 15.5%) and the plagioclase phyric basalt having elevated Al₂O₃ (19.3%) and CaO (13.1%) contents. Compositional variations are extremely consistant, with the olivine phyric basalts and the sparsely plagioclase phyric basalts defining coherent linear trends. On the basis of the ratios and covariation of the incompatible trace elements Zr, Nb, Y and Ba, distinct parental magmas for each basalt type are required. An investigation of Fe-Mg and Mg-Ni distribution coefficients between olivine and magma indicates that olivines from the olivine phyric basalts are on average too forsteritic and too Ni poor to have crystallized in a magma corresponding to the host bulk rock composition. This implies that these basalts are enriched in xenocrystic olivine. Olivines from the other basalt types are mostly of equilibrium composition, although there are some exceptions. Petrogenetic models for the formation of the different basalt types are quantitatively evaluated in terms of fractional crystallization/crystal accumulation processes. These indicate that (1) the olivine phyric basalts are the products of olivine and minor Cr-spinel accumulation and do not represent analogues of primary magma, or a liquid fractionation trend; (2) that the sparsely plagioclase phyric basalts were formed by polybaric fractional crystallization of olivine, plagioclase and clinopyroxene; and (3) that the evolved sparsely plagioclase phyric basalts are not readily related to one another. The single highly plagioclase phyric basalt is unrelated to the other basalt types and is cumulus enriched in plagioclase.The different basalt types are unrelated to one another and document the presence of at least four distinct magma types erupted in close proximity at this ridge/transform intersection on the southern end of the Mid-Atlantic Ridge.     

RARE EARTH ELEMENT CONCENTRATIONS IN USGS AND CCRMP IGNEOUS ROCK STANDARDS DETERMINED BY HIGH PERFORMANCE ION CHROMATOGRAPHY

Eleven rare earth elements (REE) have been determined in ten USGS and two CCRMP igneous rock standards using high performance ion chromatography. Duplicate analyses of two or more separately prepared aliquots of each sample were undertaken; reproducibility was better than 5% for each of the REE. In most cases, the results compare closely with published REE values for those samples for which the REE are well characterised. Where the results for individual elements are in disagreement with published data, the more erratic patterns yielded by the earlier data when plotted on chondrite-normalised REE diagrams suggest that values determined in this study are of greater accuracy.     

The main mean motion commensurabilities in the planar circular and elliptic problem

We study the motion of asteroids in the main mean motion commensurabilities in the frame of the planar restricted three-body problem. No assumption is made about the size of the eccentricity of the asteroid. At small to moderate eccentricity, we recover existing results (shape of the phase space and location of secondary resonances). We also provide global pictures of the dynamics in the region of secondary resonances. At high eccentricity, the phase space portraits of the integrable part of the Hamiltonian show new families of stable orbits for the 3:2 and 2:1 cases and the secular resonances ₅ and ₆ are located.     

Jason-1 Altimeter Ground Processing Look-Up Correction Tables

Poseidon-2 is the dual frequency radar altimeter embarked on the CNES/NASA oceanographic satellite Jason-1 that was launched on 7 December 2001. The primary objective of the Jason-1 mission is to continue the high accuracy time series of altimeter measurements that began with TOPEX in 1992. To achieve this goal, it is necessary to improve each component of the ground processing continually. Among these components are the look-up correction tables that are used to correct the estimations (range, significant waveheight, and sigma naught) issued from the retracking algorithms (on-board and ground). Look-up tables were first computed taking into account the prelaunch characteristics of the altimeter. They have to be updated to take into account better all the in-flight characteristics of the altimeter and all the updated ground algorithms that can impact the estimation process. The aim of this article is to describe the radar altimeter simulator of performances that has been used to compute look-up tables, to display the freshly computed look-up tables, and to discuss the consequences of these new corrections on the products provided to the users. The updated look-up correction tables allow improvement of SWH estimation, in particular with respect to TOPEX SWH data. It is also shown that no range dependency on SWH has to be looked for in these tables, and that the on-board TOPEX and Poseidon-2 tracking systems may contain the differences explaining the relative sea state bias between both altimeters.     

Helium–carbon relationships in geothermal fluids of western Anatolia,Turkey

We investigate the helium, carbon and oxygen–hydrogen isotopic systematics and CO₂/³He ratios of 8 water and 6 gas samples collected from 12 geothermal fields in western Anatolia (Turkey). ³He/⁴He ratios of the samples (R) normalized to the atmospheric ³He/⁴He ratio (R_A = 1.39 × 10^? 6) range from 0.27 to 1.67 and are significantly higher than the crustal production value of 0.05. Fluids with relatively high R / R_A values are generally found in areas of significant heat potential (K?z?ldere and Tuzla fields). CO₂/³He ratios of the samples, ranging from 1.6 × 10⁹ to 2.3 × 10¹⁴, display significant variation and are mostly higher than values typical of an upper mantle source (2 × 10⁹). The δ¹³C (CO₂) and δ¹³C (CH₄) values of all fluids vary from ? 8.04 to + 0.35‰ and ? 25.80 to ? 23.92‰ (vs. PDB), respectively. Stable isotope values (δ¹⁸O–δD) of the geothermal waters are conformable with the Mediterranean Meteoric Water Line and indicate a meteoric origin. The temperatures calculated by gas geothermometry are significantly higher than estimates from chemical geothermometers, implying that either equilibrium has not been attained for the isotope exchange reaction or that isotopic equilibration was disturbed due to gas additions en route to the surface.Evaluation of He–CO₂ abundances indicates that hydrothermal degassing and calcite precipitation (controlled probably by adiabatic cooling due to degassing) significantly fractionate the elemental ratio (CO₂/³He) in geothermal waters. Such processes do not affect gas phase samples to anywhere near the same extent. For the gas samples, mixing between mantle and various crustal sources appears to be the main control on the observed He–C systematics: however, crustal inputs dominate the CO₂ inventory. Considering that limestone is the main source of carbon (～ 70 to 97% of the total carbon inventory), the carbon flux from the crust is found to be at least 20 times that from the mantle. As to the He-inventory, the mantle-derived component is found to vary up to 21% of the total He content and is probably transferred to the crust by fluids degassed from deep mantle melts generated in association with the elevated geotherm and adiabatic melting accompanying current extension. The range of ³He/enthalpy ratios (0.000032 to 0.19 × 10^? 12 cm³ STP/J) of fluids in western Anatolia is consistent with the release of both helium and heat from contemporary additions of mantle-derived magmas to the crust. The deep faults appear to have facilitated the deep circulation of the fluids and the transport of mantle volatiles and heat to the surface.     

Controlled source electromagnetic three‐dimensional grid‐modelling based on a complex resistivity structure of the seafloor: effects of acquisition parameters and geometry of multi‐layered resistors

A comprehensive controlled source electromagnetic (CSEM) modelling study, based on complex resistivity structures in a deep marine geological setting, is conducted. The study demonstrates the effects of acquisition parameters and multi‐layered resistors on CSEM responses. Three‐dimensional (3D) finite difference time domain (FDTD) grid‐modelling is used for CSEM sensitivity analysis. Interpolation of normalized CSEM responses provides attributes representing relative sensitivity of the modelled structures. Modelling results show that fine grid, 1 × 1 km receiver spacing, provides good correlations between CSEM responses and the modelled structures, irrespective of source orientation. The resolution of CSEM attributes decreases for receiver spacing >2 × 2 km, when using only in‐line data. Broadside data in the grid geometry increase data density by 100 – approximately 200% by filling in in‐line responses and improve the resolution of CSEM attributes. Optimized source orientation (i.e., oblique to the strike of an elongated resistor) improves the structural definition of the CSEM anomalies for coarse‐grid geometries (receiver spacing ≥3 × 3 km). The study also shows that a multi‐resistor anomaly is not simply the summation but a cumulative response with mutual interference between constituent resistors. The combined response of constituent resistors is approximately 50% higher than the cumulative response of the multi‐resistor for 0.5 Hz at 4000 m offset. A gradual inverse variation of offset and frequency allows differentiation of CSEM anomalies for multi‐layered resistors. Similar frequency‐offset variations for laterally persistent high‐resistivity facies show visual continuity with varying geometric expressions. 3D grid‐modelling is an effective and adequate tool for CSEM survey design and sensitivity analysis.