Effects of 17beta-estradiol exposure in the mussel Mytilus galloprovincialis

Mussels Mytilus galloprovincialis were exposed to different concentrations of estradiol (20, 200, and 2000 ng/l) in a semi-static regime (1-day dosing intervals) for up to 7 days in an attempt to see how mussels dealt with exogenous estrogenic compounds. Sex hormone levels were determined in whole tissue. Free-estradiol was only significantly elevated at the highest exposure dose (up to 10-fold). Most of the estradiol was in the tissues as fatty acid esters (> 78%), which sharply increased in a dose-dependent manner (from 4 ng/g in controls to 258 ng/g at the high exposure group). In contrast, neither free nor esterified testosterone levels showed significant differences between control and exposure groups. The results suggest the existence of mechanisms that allow mussels to maintain their hormonal status, and the important role that fatty acid esterification may play within those mechanisms. Synthesis and conjugation rates of estradiol were further investigated by measuring the activity of P450 aromatase, and palmitoyl-CoA:estradiol acyltransferase, in digestive gland microsomal fractions. Overall, the study contributes to the better knowledge of molluscan endocrinology, and defines new mechanisms of regulation of free steroid-levels in mussels.     

Effects of 17β-estradiol exposure in the mussel Mytilus galloprovincialis

Mussels Mytilus galloprovincialis were exposed to different concentrations of estradiol (20, 200, and 2000 ng/l) in a semi-static regime (1-day dosing intervals) for up to 7 days in an attempt to see how mussels dealt with exogenous estrogenic compounds. Sex hormone levels were determined in whole tissue. Free-estradiol was only significantly elevated at the highest exposure dose (up to 10-fold). Most of the estradiol was in the tissues as fatty acid esters (>78%), which sharply increased in a dose-dependent manner (from 4 ng/g in controls to 258 ng/g at the high exposure group). In contrast, neither free nor esterified testosterone levels showed significant differences between control and exposure groups. The results suggest the existence of mechanisms that allow mussels to maintain their hormonal status, and the important role that fatty acid esterification may play within those mechanisms. Synthesis and conjugation rates of estradiol were further investigated by measuring the activity of P450 aromatase, and palmitoyl-CoA:estradiol acyltransferase, in digestive gland microsomal fractions. Overall, the study contributes to the better knowledge of molluscan endocrinology, and defines new mechanisms of regulation of free steroid-levels in mussels.     

Residues of C-4n-nonylphenol in mosquitofish (Gambusia holbrooki) oocytes and embryos during dietary exposure of mature females to this xenohormone

In order to assess in fish the maternal transfer of alkylphenolic compounds to the progeny, the identification and quantification of the labelled compounds present in oocytes and embryos was conducted after dietary exposure of mature female mosquitofish to ¹⁴C-4n-nonylphenol during vitellogenesis and embryogenesis respectively. Radioactivity found in bile and liver extracts accounted for 0.9–0.6 and 0.2–0.1% of ingested radioactivity for females exposed during vitellogenesis and embryogenesis respectively. The amount of extractable radioactivity present in oocytes and embryos was 0.19 and 0.07% of the ingested dose respectively. The radio-HPLC profiles obtained from bile, liver, oocyte and embryo extracts were similar. They showed the presence of 4n-NP-glucuronide as the major metabolite and traces of unchanged 4n-NP. The other metabolites corresponded to 8-hydroxynonylphenol, 9-(4-hydroxyphenyl)-nonanoic acid and para-hydroxybenzoic acid which is the final product of the alkyl chain oxidation. Our results indicate that exposure of ovoviviparous female fish to 4-NP during vitellogenesis and embryogenesis leads to the contamination of the progeny by 4-NP and its metabolites.     

Protostellar evolution during time-dependent anisotropic collapse

The formation and collapse of a protostar involves the simultaneous infall and outflow of material in the presence of magnetic fields, self-gravity and rotation. We use self-similar techniques to self-consistently model the anisotropic collapse and outflow by using a set of angle-separated self-similar equations. The outflow is quite strong in our model, with the velocity increasing in proportion to radius, and material formally escaping to infinity in the finite time is required for the central singularity to develop.
Analytically tractable collapse models have been limited mainly to spherically symmetric collapse, with neither magnetic field nor rotation. Other analyses usually employ extensive numerical simulations, or either perturbative or quasistatic techniques. Our model is unique as an exact solution to the non-stationary equations of self-gravitating magnetohydrodynamics (MHD), which features co-existing regions of infall and outflow.
The velocity and magnetic topology of our model is quadrupolar, although dipolar solutions may also exist. We provide a qualitative model for the origin and subsequent evolution of such a state. However, a central singularity forms at late times, and we expect the late-time behaviour to be dominated by the singularity, rather than depend on the details of its initial state. Our solution may, therefore, have the character of an attractor among a much more general class of self-similarity.     

Long-term decline of the populations of Fucales (Cystoseira spp. and Sargassum spp.) in the Albères coast (France, North-western Mediterranean)

Only five of fourteen species of Fucales reported at the end of the XIXth century are currently present in the Albères Coast (France, NW Mediterranean). According to historical data there has been a steady decrease of all the populations since the 1940s. Seven taxa now extinct (Cystoseira crinita, Cystoseira barbata, Cystoseira foeniculacea f. tenuiramosa, Cystoseira spinosa, Cystoseira spinosa var. compressa, Sargassum hornschuchii and Sargassum vulgare) were considered frequent and some of them were the dominant and engineering species in several phytobenthic assemblages. Moreover, only one of the five species left, shows no signs of regression (Cystoseira compressa), two are considered as rare (Cystoseira caespitosa, Cystoseira zosteroides), and one is very rare (Cystoseira elegans). Cystoseira mediterranea, a species that was reported to make a continuous belt along the shores of the Albères coast, has almost disappeared from some areas. Overgrazing by sea urchins, outcompetition by mussels, habitat destruction, scientific research sampling and, probably, human trampling and chemical pollution are to be blamed for the decline of populations thriving in shallow waters. Deep-water species have been affected by an increase in water turbidity and, probably, chemical pollution and direct plant destruction attributed to net fishing. If degradation of the environmental conditions continues, the remaining Cystoseira species will face a most unwelcome prospect. Even after the removal of the causes that led to its die-off, natural restoration of extinct species seems not to be possible because the decline has also affected populations from nearby areas and zygotes are unable to disperse over long distances. Urgent management actions have to be designed in order to improve the current situation of the populations of Fucales in the Albères coast.     

Indicator-Based Geostatistical Models For Mapping Fish Survey Data

Marine research survey data on fish stocks often show a small proportion of very high-density values, as for many environmental data. This makes the estimation of second-order statistics, such as the variance and the variogram, non-robust. The high fish density values are generated by fish aggregative behaviour, which may vary greatly at small scale in time and space. The high values are thus imprecisely known, both in their spatial occurrence and order of magnitude. To map such data, three indicator-based geostatistical methods were considered, the top-cut model, min–max autocorrelation factors (MAF) of indicators, and multiple indicator kriging. In the top-cut and MAF approaches, the variable is decomposed into components and the most continuous ones (those corresponding to the low and medium values) are used to guide the mapping. The methods are proposed as alternatives to ordinary kriging when the variogram is difficult to estimate. The methods are detailed and applied on a spatial data set of anchovy densities derived from a typical fish stock acoustic survey performed in the Bay of Biscay, which show a few high-density values distributed in small spatial patches and also as solitary events. The model performances are analyzed by cross-validating the data and comparing the kriged maps. Results are compared to ordinary kriging as a base case. The top-cut model had the best cross-validation performance. The indicator-based models allowed mapping high-value areas with small spatial extent, in contrast to ordinary kriging. Practical guidelines for implementing the indicator-based methods are provided.