基于SLAF-seq技术的黑棘鲷微卫星标记开发及其在鲷科鱼类中的通用性研究

黑棘鲷(Acanthopagrusschlegelii)是广泛分布于西北太平洋的暖温性中下层经济鱼类,也是我国沿海重要的海洋捕捞鱼类和增养殖对象。然而,目前有关黑棘鲷的微卫星标记研究报道较少,难以对其种质资源状况作出精确评估。本研究采用SLAF-seq技术测序共获得22489个二至六碱基重复的黑棘鲷微卫星序列,短重复序列(二、三碱基)占总微卫星序列的90.8%,长重复序列(四至六碱基)占有9.2%。经过157对随机合成引物的多态性筛选,开发出49个高多态性的黑棘鲷微卫星标记,其中短重复序列位点有25个,长重复序列位点有24个。每个位点的等位基因数(Na)为2—20(均值为8.3),观测杂合度(Ho)和期望杂合度(He)分别为0.097—0.938和0.122—0.922(均值分别为0.663和0.701),多态信息含量(PIC)为0.118—0.897(均值为0.655)。经Bonferroni校正后,有47个位点符合哈迪-温伯格平衡(HWE),各位点间未检测到连锁不平衡现象,仅2个位点偏离HWE。结果表明,所开发的大部分微卫星标记具有高多态性,蕴含的遗传信息含量较为丰富,能够为黑棘鲷的种群遗传资源评估提供数量充足、类型多样的有效分子标记。跨物种扩增结果显示,有43个黑棘鲷微卫星标记可在9种鲷科鱼类中成功扩增,其中28个标记在太平洋棘鲷(Acanthopagruspacificus)、黄鳍棘鲷(Acanthopagruslatus)和澳洲棘鲷(Acanthopagrusaustralis)中具有较好的通用性,2个标记在平鲷(Rhabdosargussarba)、蓝点赤鲷(Pagruscaeruleostictus)、真赤鲷(Pagrusmajor)、二长棘犁齿鲷(Evynnis cardinalis)及黄牙鲷(Dentex hypselosomus)中具有通用性。这些通用性标记可为阐明鲷科属、种间的系统进化关系和棘鲷属鱼类的群体遗传学分析提供新的标记来源和研究角度。     

空间分辨率对中国夏季降水时空演变特征的影响

本文使用1979—2021年国家气候中心160站(R₁₆₀)和国家级地面气象观测站2 314站(R₂₃₁₄)逐月降水观测资料,利用EOF (Empirical Orthogonal Function)分解和相关分析等方法,研究两类资料夏季降水时空变化特征及其与海气因素之间物理联系的表征水平差异,并分析了差异的可能原因。结果表明,R₁₆₀在我国西北、青藏高原等地区站点极为稀疏,导致其各EOF模态对上述地区降水的时空变化特征描述失真,中东部地区偏低的空间分辨率会使局地强降水的变化特征信号损失,造成降水的年际变率降低。而R₂₃₁₄主模态能够更为真实地反映我国降水的时空演变特征,特别是在极端降水频发的江淮地区以及降水受局地地形影响较大的山区,其EOF主模态的空间分布和时间系数演变与ENSO (El Niño-Southern Oscillation)、西太平洋副热带高压、青藏高原雪盖等气候强迫信号之间的相关性更为显著。     

Photometric Follow-up Transit (Primary Eclipse) Observations of WASP-43 b and TrES-3b and A Study on Their Transit Timing Variations

Two photometric follow-up transit (primary eclipse) observations on WASP-43 b and four observations on TrES-3 b are performed using the Xuyi Near-Earth Object Survey Telescope. After differential photometry and light curve analysis, the physical parameters of the two systems are obtained and are in good match with the literature. Combining with transit data from a lot of literature, the residuals (O ? C) of transit observations of both systems are fitted with the linear and quadratic functions. With the linear fitting, the periods and transit timing variations (TTVs) of the planets are obtained, and no obvious periodic TTV signal is found in both systems after an analysis. The maximum mass of a perturbing planet located at the 1:2 mean motion resonance (MMR) for WASP-43 b and TrES-3 b is estimated to be 1.826 and 1.504 Earth mass, respectively. By quadratic fitting, it is confirmed that WASP-43 b may have a long-term TTV which means an orbital decay. The decay rate is shown to be P? = (?0.005248 ± 0.001714) s·yr^?1, and compared with the previous results. Based on this, the lower limit of the stellar tidal quality parameter of WASP-43 is calculated to be ${Q^{\prime }}_{*}\ge 1.5\times {10}^5$, and the remaining lifetimes of the planets are presented for the different ${Q^{\prime }}_{*}$ values of the two systems, correspondingly.     

基于闪锌矿晶体中受激电磁耦子散射产生可调谐太赫兹波的理论研究

基于受激电磁耦子散射原理,采用已报道的利用非线性光学参量振荡方法产生可调谐太赫兹波的实验条件作为理论分析的实验模型,以GaAs,GaP, InP,ZnTe晶体为代表,计算分析了在闪锌矿晶体中参量振荡产生太赫兹波的吸收、增益特性,对输出THz波的调谐特性给出了详尽分析.分析太赫兹波高效耦合输出的腔型结构,并与掺氧化镁铌酸锂晶体组成的太赫兹波参量振荡器做对比.     

非均一相互作用能对超薄膜生长影响的Monte Carlo模拟研究

利用Monte Carlo (MC)模拟技术研究了非均一的吸附原子与基底相互作用能在一定的生长条件下对超薄膜生长过程的影响.非均一相互作用能是由基底表面原子在垂直和水平方向上实际位置与理想晶格原子位置的偏差所造成.本文用高斯分布来表示这种非均一相互作用能.模拟结果表明:非均一相互作用能对超薄膜的生长过程及薄膜的形貌有显著的影响.这种影响同时受到生长条件的限制,在中等温度时相互作用能的非均一性对岛的个数、平均大小的影响最显著;温度的增加在一定程度上可抵御相互作用能的非均一性对薄膜生长的影响.     

不同模式模拟的降水稳定同位素效应的比较和评估

利用引入水稳定同位素循环的ECHAM4、GISS E、HadCM3、MUGCM以及iAWBM的模拟数据,分析了全球降水中稳定同位素效应的空间分布特征,对不同模式的模拟结果之间以及模拟结果与GNIP（Global Network of Isotopes in Precipitation）的实际监测结果之间进行了比较,旨在对稳定同位素大气环流模式的模拟有效性进行评价,改善对水循环中水稳定同位素效应的理解和认识。结果显示,5个模式均很好地再现了全球降水中平均δ18O和平均δ18O季节差的空间分布特征,降水中稳定同位素的温度效应、降水量效应的分布特点以及全球大气水线GMWL（Global Meteoric Water Line）均被很好地模拟出。比较而言,ECHAM4模拟的降水中的平均δ18O以及δ18O平均季节差的空间分布与GNIP的实际分布最接近,拟合水平也最高;ECHAM4、GISS E、MUGCM和iAWBM再现全球温度效应空间分布的能力较强,拟合水平大致相当;由iAWBM模拟的降水量效应空间分布与实际分布之间的相关性最强,5个模式模拟的与实测的δ18O/P相关系数符号相同的站点数大致位于同一水平;GISS E和iAWBM模拟的全球大气水线与实测的GMWL最接近。     

A method for aligning the plastic scintillator detector on DAMPE

The Plastic Scintillator Detector(PSD) onboard the DArk Matter Particle Explorer(DAMPE)is designed to measure cosmic ray charge(Z) and to act as a veto detector for gamma ray identification.To fully exploit the charge identification potential of PSD and to enhance its capability to identify gamma ray events, we develop an alignment method for the PSD. The path length of a given track in the volume of a PSD bar is derived taking into account the shift and rotation alignment corrections. By examining energy spectra of corner-passing events and fully contained events, position shifts and rotations of all PSD bars are obtained, and are found to be on average about 1 mm and 0.0015 radian respectively. To validate the alignment method, we introduce artificial shifts and rotations of PSD bars into the detector simulation.These shift and rotation parameters can be recovered successfully by the alignment procedure. As a result of the PSD alignment procedure, the charge resolution of the PSD is improved from 4% to 8%, depending on the nuclei.     

Analysis of 1989/1990 main physical fields for air-sea coupled system in the tropical western Pacific

In this paper, the marine ship observation data sets in the seventh (October 16-December 4. 1989) and eighth (June 1-July 16, 1990) cruises of PRC -US tropical ocean and global atmosphere (TOGA) joint scientific investigation in the tropical western Pacific are used to analyze the elements such as sea surface temperature (SST), surface wind field, fluxes and net heat budget, which are important physical parameters of underlying earth's surface influencing the global mean circulation evolution on seasonal and interannual time scales. These diagnostic analyses are very beneficial to the understanding of the regional climate characteristics and the air-sea interaction mechanism, and the improving of surface flux parameterizations and regional or global climate model.     

烟台芝罘湾流场特征的分析

基于1982年4～6月海流观测资料,本文分析了芝罘湾流场的基本特征。结果表明,流场以潮流为主。整个海湾,湾口流速最大,湾底流速较小。除湾中央区外,潮流基本属于往复流型。与潮流相比,余流较小,量值大多为7cm/s左右。     

EEA——一种新的、重要的海洋生态学参数及其应用

细菌胞外酶作为海洋环境中有机物与细菌相互作用的中介，其活性高低反映了细菌对相关有机组分的改造程度及该有机组分对细菌生长繁殖的营养贡献。胞外酶活性（extracellular enzyme activity,EEA)研究对于深入了解海洋生态系有着重要意义。本文概述了胞外酶活性特性、影响因素及调控机制；台湾海峡胞外酶活性研究原理，现场测定方法及影响因素，分析了台湾海峡β-葡萄糖苷酶与细菌产量、生物量的关系。