UV-B辐射对海洋微藻抗氧化系统的影响

研究了UV－B辐射对两种海洋微藻－小新月菱形藻（Ntzschia closterlum)和亚心形扁藻（Platymonas subcordiforming)的抗氧化系统的影响。结果表明：在本实验的辐射剂量范围内，随着UV－B辐射剂量的增强，两种藻的抗氧化防御系统的非酶性组分还原型谷胱甘肽（GSH）和类胡萝卜素（CAR）的含量随之下降；抗氧化防御系统的酶性组分超氧化歧化酶（SOD）和过氧化氢酶（CAT）的活性逐渐降低。加入外源性抗氧化剂GSH可减轻UV－B辐射对藻的伤害。分析表明海洋微藻的UV－B伤害是由于抗氧化防御系统遭到破坏而导致活性氧积累的结果。     

斜向波对直立式防波堤堤头作用的试验研究

直立式防波堤堤头在斜向波浪作用下波浪力的计算在现行的技术规范中尚属空白。通过物理模型试验给出了堤头结构设计所需的波压分布图，指出了波浪入射方向和地形对堤头所受波浪力的贡献有时可以超过波高和波周期，根据现行规范按波浪正向入射计算堤头所受的波浪力偏于安全。     

Regulation of Tilapia metallothionein gene expression by heavy metal ions

Tilapia is a common fish species inhabiting inland waters and estuarine regions in Hong Kong and Southeast Asia, and useful for bio-monitoring of metal pollution. Metallothionein (MT) gene expression in fish tissues has been useful to sub-lethal risk assessment as biomarker of exposure to metal ions in fishes inhabiting metal contaminated area. To investigate metal inductions of Tilapia MT gene expression in vivo, Tilapias were injected with different concentrations of heavy metals and tissues were then removed for quantitative PCR assay using mimic PCR methods. All of the metal ions tested (Cu(2+), Cd(2+), Hg(2+), Ni(2+), Pb(2+) and Zn(2+)) were able to induce hepatic MT mRNA levels. Renal MT mRNA levels of Cd(2+) and Zn(2+) treated fish was not induced with significant fold induction, however MT mRNA levels in gills were sensitive to the administrations of these metal ions. These data indicated that Tilapia MT mRNA levels in gills and liver are sensitive biomarker of exposure to various metal ions.     

网络与计算机应用实时业务运行系统

本文主要介绍国家海洋环境预报中心网络与计算机应用的实时通信系统、实时数据处理系统、实时预报发布系统等目前实时运行的十项业务系统.     

A New Probability of Detection Model for Updating Crack Distribution of Offshore Structures

1 .IntroductionNondestructiveinspection (NDI)isveryimportantforensuringthereliabilityofoffshorestructuresintheirservicelives (Lauraetal.,1 996 ) .Itiswellknownthatdetectionofflawsinvolvesconsider ablestatisticaluncertainties.Asaresult,theprobabilityofdetection (POD)forallflawsofagivensizehasbeenusedintheliteraturetodefinethecapabilityofaparticularNDItechniqueinagivenen vironment.SincethedataofPODusuallyscatterlargely ,itisdifficulttodeterminewhichmodelfitstheavailabledatabest.Thismodelun…     

高压技术在深海沉积物兼性嗜压菌的筛选和鉴定中的应用

通过自行改进的高压培养罐及高压设备,对深海沉积物进行可培养微生物的筛选,获得6株具有较强耐受压力的细菌.16SrDNA的测序结果表明这些细菌分别属于6个不同的菌属.压力生长试验的结果表明这6株细菌在40MPa的条件下仍然具有较强的生长能力,属于兼性嗜压菌.对不同压力下生长的细菌做显微镜检,结果显示,除了一株芽孢杆菌在40MPa下的菌体形态发生了明显的变化外,其它5株细菌在压力条件下的菌体的分裂均没有受到明显的影响.     

中国对虾染色体制备及染色体形态的研究

以中国对虾原肠期胚胎为材料，采用整体低渗法和管渗法均制备了染色体。通过比较发现，管渗法制备的染色体比整体低渗法的形态好。本文对制备中国对虾染色体技术方面的问题进行了探讨。通过作者的观察，了解到中国对虾染色体徐正常特征外，还存在较特别的形态特征，如主缢痕增强，次缢痕变化，同源配对等。并对产生这些形态特征的原因进行了解释。     

Sea ice characteristics between the middle Weddell Sea and the Prydz Bay, Antarctica during the austral summer of 2003

The antarctic sea ice was investigated upon five occasions between January 4 and February 15, 2003. The investigations included: (1) estimation of sea ice distribution by ship-based observations between the middle Weddell Sea and the Prydz Bay; (2) estimation of sea ice distribution by aerial photography in the Prydz Bay; (3) direct measurements of fast ice thickness and snow cover, as well as ice core sampling in Nella Fjord; (4) estimation of melting sea ice distribution near the Zhongshan Station; and (5) observation of sea ice early freeze near the Zhongshan Station. On average, sea ice covered 14.4% of the study area. The highest sea ice concentration (80%) was observed in the Weddell Sea. First-year ice was dominant (99.7%-99.8%). Sea ice distributions in the Prydz Bay were more variable due to complex inshore topography, proximity of the Larsemann Hills, and/or grounded icebergs. The average thickness of landfast ice in NeUa Fjord was 169.5 cm. Wind-blown snow redistribution plays an important role in affecting the ice thickness in Nella Fjord. Preliminary freezing of sea ice near the Zhongshan Station follows the first two phases of the pancake cycle.     

三维斜压模式对冬季南海环流的数值计算

用一个三维、自由表面、原始方程模式对南海环流进行了计算.计算结果表明:黑潮在巴士海峡以西呈一反气旋弯曲流动路径,有一相对高温高盐的水舌从巴士海峡伸入南海,表明有部分黑潮水侵入南海.冬季南海的一些观测事实在模式结果里得到了很好的反映,像冬季逆风向东北方向流动的“南海暖流”和一些中尺度涡旋.同时还分析了巴士海峡沿120.75°E断面的流速和盐度的垂直结构,并同观测结果进行了比较.根据模式结果,我们还进一步讨论了“南海暖流”的形成和驱动机制.     

摄食水平和性别对稀有鮈鲫生长和能量收支的影响

于1995年10月采集本所自繁有ju鲫（约1.03g/ind)，在30℃水温下进行摄食－生长实验，研究了从饥饿到饱食5个摄食水平对不同性别稀有ju鲫的生长和能量收支的影响，结果表明，鱼体干物和能量含量不受摄食率和性别的影响，随着摄食率的增加，雌鱼的湿重，干重和能量特定生长率均呈双对数增加，雄鱼湿重特定生长率呈双对数增加，而干物质和能量特定生长率呈指增加，雄鱼生长速度及摄食率低于雌鱼，随着摄食率的增加，食物能损失于粪便中的比例均呈上升趋势，损失于排泄物中的比例变化不大，呈下降趋势，用于代谢的比例呈下降趋势，用于生长的比例呈上升趋势，除饥饿组外，在各个摄食水平，雌鱼食物能用于代谢的比例较雄鱼低，而用于生长的比例较雄鱼高。