Genetic diversity and differentiation of three populations of Penaeus monodon Fabricus

Genetic diversity of two wild Penaeus monodon populations sampled from the coastal waters of Qinglan （ Hainan Province of China, HN） and Malaysia （KD）, and the F1 generation of a Thailand broodstock population （CP） were examined by vertical polyacrylamide gel electrophoresis. Of 21 loci encoded by ten enzymes, 11 were polymorphic. The mean proportions of polymorphic loci of HN, KD and CP were 36. 36%, 45.45% and 50.00% , with the average heterozygosities of 0.135, 0.181 and 0.191, and the effective numbers of alleles per loci were 1. 300, 1. 330 and 1. 329, respectively. The divergent indexes of HN, KD and CP were0.023, 0.124, and 0.117, respectively. The genetic distance between the two wild populations was 0.005, and the gene differentiation coefficient was also very low （0.014）. The results indicated that the F1 generation population had a higher genetic diversity than the two wild populations, and that the HN population had the lowest one. There was no significant differentiation between HN and KD populations and all populations existed with a slight heterozygote excess.     

基于GIS技术的耕地坡度分级方法

分析传统耕地坡度分级方法的缺陷,提出利用GIS技术的耕地坡度分级新方法。以枝江市董市镇为例实证,研究制定定量融合指标和工作流程,不仅有效地控制了耕地图斑的数量,而且确保利用耕地坡度分级数据统计汇总的各耕地坡度级面积数据与真实值基本一致,提高了汇总面积数据的精度。     

东北季风期海南岛三亚湾初级生产力分布特征及其影响因素

根据2006年10月和2007年1月的监测资料,对三亚湾在东北季风初始期和盛行期初级生产力的分布特征及其受水体环境的影响因素进行了分析。三亚湾秋、冬季表层潜在初级生产力平均值分别为6.81mgC·m-3·h-1和7.47mgC·m-3·h-1;水柱初级生产力平均值分别为504.96mgC·m-2·d-1和495.33mgC·m-2·d-1。东北季风期间,三亚湾水体垂直混合作用加强,层化现象减弱,水动力过程和营养盐等环境因子是该海湾初级生产力分布特征和季节变化的重要调控因素;其中冬季较高的DIN分布对表层初级生产力有潜在促进作用,而水体扰动增强、真光层深度下降则导致水柱初级生产力低于秋季。三亚河口近岸水域秋、冬季均为营养盐、叶绿素a及初级生产力的高值区,其中表层潜在初级生产力在秋季和冬季分别达到了45.80mgC·m-3·h-1和39.27mgC·m-3·h-1,表明调查期间三亚河营养盐输入对近岸水体浮游植物初级生产过程的显著影响。     

雨生红球藻八氢番茄红素脱氢酶pds基因上游序列的分离和分析

在某些蓝藻、绿藻和高等植物中,八氢番茄红素脱氢酶是β-胡萝卜素合成过程中的一个关键酶之一,应用巢式PCR方法从雨生红球藻中克隆了八氢番茄红素脱氢酶基因约1kb的5’上游序列。通过生物信息学方法进行序列分析,发现pds基因上游序列中包含了一些可能的顺式元件,如ABRE调控元件、C-repeat/DRE调控元件等。同时,构建了由pds-启动子控制报告基因的重组载体,并转化到雨生红球藻细胞中,进行了报告基因瞬间表达的检测。结果表明,克隆的pds启动子区域具有启动子活性,可以驱使报告基因进行瞬间表达。     

Establishment and application of an intelligent treating method for oil spill identification

In the identifying process of an oil spill accident, manual integral and artificial visual comparison are commonly used at present to determine the oil spill sources, these methods are time-consuming and easily affected by human factors. Therefore, it is difficult to achieve the purpose of rapid identification of an oil spill accident. In this paper, an intelligent method of automatic recognition, integration and calculation of diagnostic ratio of Gas Chromatography-Mass Spectrometer (GC/MS) spectrum are established. Firstly, four hundreds of samples collected around the world were analyzed using a standard method and Retention time locking technology (RTL) was applied to reduce the change of retention time of GC/MS spectrum. Secondly, the automatic identification, integration of n-alkanes, biomarker compounds, polycyclic aromatic hydrocarbons and calculation of the diagnostic ratios were realized by MATLAB software. Finally, a database of oil fingerprints were established and applied successfully in a spill oil accident. Based on the new method and database, we could acquire the diagnostic ratios of an oil sample and find out the suspected oil within a few minutes. This method and database can improve the efficiency in spilled oil identification.     

基于机器学习的格点气温预报订正方法

使用2017年9月至2021年3月国家级业务化运行的智能网格实况分析产品和欧洲中期天气预报中心全球模式(EC)产品,根据湖北省的地理分布特征构建6个分区,采用基于LightGBM机器学习算法建立的气温预报方法,生成湖北省0.05°×0.05°格点气温预报产品。利用2021年4—9月的预报产品和格点实况资料进行检验,结果表明:基于机器学习的气温预报方法(MLT)取得了较好的预报效果,其在0～72 h时效内优于中央气象台下发的气温精细化指导预报(SCMOC)和EC产品;MLT在山区的误差较平原大,但山区的订正幅度大于平原,日最高气温的订正幅度大于日最低气温的订正幅度;4—9月MLT、SCMOC、EC产品的平均绝对误差(MAE)日变化都呈现了白天偏高、夜间偏低、午后凸起的单峰特征,MLT的MAE值较SCMOC和EC产品的更低,并且在转折性天气中仍具有优势;站点检验与格点检验结论一致,基于格点建模的气温预报产品对站点预报同样得到了订正。机器学习在格点气温的模式订正方面可以作为一个行之有效的手段。     

土壤墒情观测常见问题及排除经验

针对土壤墒情观测编发报及应用中常遇到的问题,寻找问题的排除方法。     

区域产业升级理论评价和升级目标层次论建构

产业升级相关文献综述表明,区域产业升级具有丰富的理论含义.产业结构升级研究寻求对产业宏观结构比例的统计学意义上的优化;产业地域分工思路则考察产业分布演进的空间格局及其升级含义;企业升级思路则细化到产业内部,指出企业创新以获得更高附加值是产业升级的根本;集群研究则注意到区域产业发展的整体特征,并指出了推动产业集聚、产业联系与创新等升级含义.中国集群实践更进一步验证了集群层面集体效率的提升以及企业发展环境的和谐共进(区域发展目标)也是影响产业升级的重要方面.在讨论产业升级的经济维度与空间维度、个体层面与系统层面的基础上重新构建区域产业发展的理论框架,认为企业升级、集群升级和区域和谐3个层次构成了区域产业升级的3个目标层次,其相互衔接与互动对于区域产业发展具有重要意义.     

Crust-Mantle Electrical Structure in the Area along the Yadong-Burn Co Profile

1.IntroductionUntilnowTibetisstillthearchetypalregionwithcontinentalcollisiongoingoninit,henceitisanidealnaturallaboratoryforstudyingthegeologicevolutionofintracontinentalorogenicbeltandthecrust-mantledeepprocesses,aswellastheinteractionbetweenthemandthesupracrustalmovements.Forthisreason,forseveraldecadesgeologistsandgeophysicistsfromvariouscountrieshavemadeunremittingeffortsinthisregiontoprobeintotheoriginandprocessoftheplateauformation,withavarietyofcognitionsproposed.TheCMPresultsoftheSi…     

高速铁路大断面黄土隧道二次衬砌施作时机研究

隧道工程二次衬砌施作时机是隧道界普遍关注的问题。依托正在修建的郑州-西安铁路客运专线大断面黄土隧道工程,以围岩及初期支护基本稳定速率作为二次衬砌施作时机判定指标,采用位移断面监测数据回归分析和数值分析相结合,建立基本稳定速率的理论求解方法,从而确定二次衬砌施作时机;同时,选取贺家庄隧道试验段作为工程实例,对高速铁路大断面黄土隧道二次衬砌施作时机进行研究。研究结果表明：当拱顶下沉变形速率值小于0.55 mm/d,边墙水平绝对收敛速率值小于0.11 mm/d时,即可进行二次衬砌施作。此次研究为类似工程的设计和施工提供了参考依据。