Seasonal variation in mussel Mytilus edulis digestive gland cytochrome P4501A- and 2E-immunoidentified protein levels and DNA strand breaks (Comet assay).

Mytilus edulis digestive gland microsomes were prepared from indigenous populations sampled from a clean reference site (Port Quin) and an urban-industrial contaminated site (Blackpool) in the UK. Samples were collected in March/April, May, August and December 1998. Western blot analysis was performed using polyclonal antibodies to fish CYP1A and rat CYP2E using partially purified M. edulis CYP as a positive control, to aid identification. CYP1A- and CYP2E-immunopositive protein levels showed different site-specific seasonal variation with higher levels of CYP2E determined in May (P < 0.05). At both sites, lower levels of CYP1A-immunopositive protein but not CYP2E-immunopositive protein were observed in the samples collected in December (P < 0.05). This correlated with lower levels of nuclear DNA damage (Comet assay expressed as per cent tail DNA) observed in December compared to August (P < 0.05).     

Phytoplankton, nutrients and hydrography in the frontal zone between the Southwest Indian Subtropical gyre and the Southern Ocean

A survey was made of the Southwest Indian Ocean frontal region between 30 and 50°E containing the Agulhas Return, Subtropical and Subantarctic Fronts. From CTD, SeaSoar and extracted samples the distribution of nitrate, silicate and chlorophyll a is shown to be strongly linked to the front and water mass structure, varying zonally and meridionally. Surface chlorophyll a concentrations were low to the north and south leaving a band of elevated chlorophyll between the Subtropical and Subantarctic Fronts. The low concentration of chlorophyll a to the north, in Subtropical Water, was clearly due to nitrate limitation. Between the Subtropical and Subantarctic Fronts, where the chlorophyll a concentrations were highest, the surface layer showed silicate depletion limiting diatom growth. South of the Subantarctic Front there were deep extending, low concentrations of chlorophyll a, but despite plentiful supplies of macro-nutrients and a well-stratified surface layer, high concentrations of chlorophyll a were absent. Changes from west to east were associated with the meandering of the Southern Ocean Fronts, especially the Subtropical Front, and their strength and proximity to each other. Concentrations of chlorophyll a peaked where the Agulhas Return, Subtropical and Subantarctic Fronts were in close proximity. Combined frontal structures appear to have particularly pronounced vertical stability and are associated with enhanced upwelling of nutrients and leakage of nutrients across the front. Light levels are high within the shallow stable layer. Such conditions are clearly favourable for biological growth and support the development of larger-celled phytoplankton communities.     

Description of possible causes of the formation of anomalies of the cloud field over the Atlantic-European sector in the spring of 1996

We describe one of possible mechanisms of the formation of anomalies of the cloud field over the North Atlantic and Europe by using, as an example, the processes developed in the Atlantic-European sector during the natural synoptic season in the spring of 1996. It is shown that the anomalies of the cloud field can be classified with the use of the index of the North Atlantic oscillation and the latitude of the center of the Azorean maximum. Translated by Peter V. Malyshev and Dmitry V. Malyshev     

Basalt from Louisiana continental shelf

A basalt outcrop was discovered on Alderdice Bank on the outer Louisiana continental shelf. The basalt shows an age of 76.8 ± 3.3 × 10⁶ years. Textural, mineralogical, and chemical characteristics indicate that it is an alkali basalt of shallow intrusive origin. It was probably brought to the seafloor by salt tectonics and exposed due to salt dissolution.An accurate account of the Mesozoic geologic history of the Gulf of Mexico must consider the apparent consanguinity of all magmatic rocks of the region, including the Alderdice Bank basalt, and the apparent basinward decrease in age of magmatic activities.     

Factors governing abundance of hydrolyzable amino acids in the sediments from the N.W. European Continental Margin (47–50°N)

Fifty-six samples representing 6 sediment cores taken along the N.W. European Continental Margin from the shelf, slope and abyssal plain of the Goban Spur and Meriadzek Terrace were quantitatively analysed for total hydrolyzable amino acids (THAA) and clay minerals. In descending order, the five most abundant amino acids making up more than 70% of the total were: aspartic acid, glycine, serine, alanine and glutamic acid. Clay mineral proportions were typical for the N.E. Atlantic, in order of descending abundance: illite, kaolinite, chlorite, smectite and mixed layers.The Meriadzek Terrace area is characterised by fine grain suspension sedimentation with a low pelagic carbonate input and the lowest content of THAA. In contrast, the Goban Spur transect is characterised by much higher carbonate inputs and more vigorous hydrodynamics as judged from granulometry and the high abundance of minerals of shelf and continental origin and a generally higher THAA content. The pelagic portion of THAA deposited at the sea floor is more readily mineralised during early diagenesis than the more ‘refractory', clay mineral-associated continental portion. Along this margin the average mineralization of THAA down to 25 cm in the sediment is about 54%. There is a significant affinity between chlorites and amino acids which we suggest may involve the formation of ionic bonds between the octahedral layers of the clay and the amino acids.     

Benthic Nutrient Recycling in Port Phillip Bay, Australia

Benthic chamber measurements of the reactants and products involved with biogenic matter remineralization (oxygen, ammonium, nitrate, nitrite, phosphate, silicate, TCO₂and alkalinity) were used to define solute exchange rates between the sediment and overlying water column of Port Phillip Bay, Australia. Measurements at various sites throughout the bay, conducted during the summers of 1994 and 1995, indicate that the variability in flux values within a site is comparable to year-to-year variability (±50%). Four regions of the bay were distinguished by sediment properties and the northern region was identified as having 3–30 times greater nutrient regeneration rates than the other regions. Benthic recycling accounted for 63 and 72% of the annualized N and P input, respectively, to the entire bay as determined by summing benthic, dissolved riverine, atmospheric and dissolved effluent sources. However, bay-wide sedimentary denitrification accounted for a loss of 63% of the potentially recyclable N. This fraction is higher than many other coastal regions with comparable carbon loading. Denitrification efficiency is apparently not enhanced by benthic productivity nor by bio-irrigation. The rate of bio-irrigation is negatively correlated with denitrification efficiency. Bio-irrigation was studied using radon-222 and CsCl spike injection chamber measurements. Radon fluxes from sediments in Port Phillip Bay were enhanced over the diffusive flux by 3–16 times. The modelled rate of loss of Cs from chamber water was positively correlated with radon flux enhancement results. Both methods identify regions within Port Phillip Bay that have particularly high rates of non-diffusive pore-water overlying water solute exchange.     

Treatment of Hydrodynamic Loading for Random Sea State

- In order to employ cost effective frequency domain analysis for off-shore structures treatment of hydrodynamic loading is essential. Drag and inertia dominated, resonating and antiresonating cases under random sea states are analyzed to highlight the implications and relative merits of four salient linearization techniques.     

Change in biochemical and morphological characteristics of Lonicera caerulea in tectonically active zone of the Dzhazator River Valley (Altai Mountains)

Local geophysical and geochemical anomalies affect the polymorphism of taste variations, berry shape, and content of some biologically active substances in Lonicera caerulea leaves in the tectonically active Altai Mountains (Dzhazator River basin).